Restriction map for pFN21AE1842
 (Linear) (Six-base) MAPSORT of: KIEE1842.seq  Check: 7145  from: 1  to: 1074



KIEE1842.orf

 With 182 enzymes: SgfI * 

                        January 9, 2015 15:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      62     144     623     665    1074 
   Size:         62      82     479      42     409

  Fragments arranged by size: 

                 479     409      82      62      42
 
AatII G_ACGT'C

Cuts at:      0     181    1074 
   Size:        181     893
 
AleI CACnn'nnGTG

Cuts at:      0     691    1074 
   Size:        691     383
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      70    1074 
   Size:         70    1004
 
ApaI G_GGCC'C

Cuts at:      0     115     289     343     380     745    1074 
   Size:        115     174      54      37     365     329

  Fragments arranged by size: 

                 365     329     174     115      54      37
 
AscI GG'CGCG_CC

Cuts at:      0     329    1074 
   Size:        329     745
 
AvaI C'yCGr_G

Cuts at:      0     101     324     361    1074 
   Size:        101     223      37     713

  Fragments arranged by size: 

                 713     223     101      37
 
BanI G'GyrC_C

Cuts at:      0     349     453     724     769    1074 
   Size:        349     104     271      45     305

  Fragments arranged by size: 

                 349     305     271     104      45
 
BanII G_rGCy'C

Cuts at:      0      64     115     289     343     380     434     491     745 
   Size:         64      51     174      54      37      54      57     254

Cuts at:    745    1045    1074 
   Size:        300      29

  Fragments arranged by size: 

                 300     254     174      64      57      54      54      51
                  37      29
 
BbeI G_GCGC'C

Cuts at:      0     353     457     773    1074 
   Size:        353     104     316     301

  Fragments arranged by size: 

                 353     316     301     104
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     861     895    1074 
   Size:        861      34     179

  Fragments arranged by size: 

                 861     179      34
 
BclI T'GATC_A

Cuts at:      0     476    1074 
   Size:        476     598
 
BglI GCCn_nnn'nGGC

Cuts at:      0     277     293     347     740    1074 
   Size:        277      16      54     393     334

  Fragments arranged by size: 

                 393     334     277      54      16
 
BlpI GC'TnA_GC

Cuts at:      0     991    1074 
   Size:        991      83
 
Bme1580I G_kGCm'C

Cuts at:      0     115     289     343     380     698     745    1074 
   Size:        115     174      54      37     318      47     329

  Fragments arranged by size: 

                 329     318     174     115      54      47      37
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     964    1074 
   Size:        448     516     110

  Fragments arranged by size: 

                 516     448     110
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     742    1074 
   Size:        742     332
 
BsaHI Gr'CG_yC

Cuts at:      0     178     350     454     770    1074 
   Size:        178     172     104     316     304

  Fragments arranged by size: 

                 316     304     178     172     104
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     421     451    1074 
   Size:        421      30     623

  Fragments arranged by size: 

                 623     421      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     511    1074 
   Size:        511     563
 
BseYI C'CCAG_C

Cuts at:      0      76     153     697     765    1074 
   Size:         76      77     544      68     309

  Fragments arranged by size: 

                 544     309      77      76      68
 
BsiEI CG_ry'CG

Cuts at:      0      11     212     281     404     469    1074 
   Size:         11     201      69     123      65     605

  Fragments arranged by size: 

                 605     201     123      69      65      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     173    1045    1074 
   Size:        173     872      29

  Fragments arranged by size: 

                 872     173      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     169     919    1074 
   Size:        169     750     155

  Fragments arranged by size: 

                 750     169     155
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     115     173     289     343     380     434     491 
   Size:         64      51      58     116      54      37      54      57

Cuts at:    491     698     745    1045    1074 
   Size:        207      47     300      29

  Fragments arranged by size: 

                 300     207     116      64      58      57      54      54
                  51      47      37      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62     144     623     665    1074 
   Size:         62      82     479      42     409

  Fragments arranged by size: 

                 479     409      82      62      42
 
BsrFI r'CCGG_y

Cuts at:      0     254     406     456     526     730    1074 
   Size:        254     152      50      70     204     344

  Fragments arranged by size: 

                 344     254     204     152      70      50
 
BssHII G'CGCG_C

Cuts at:      0     329     752    1074 
   Size:        329     423     322

  Fragments arranged by size: 

                 423     329     322
 
BssSI C'ACGA_G

Cuts at:      0     172    1074 
   Size:        172     902
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     939    1074 
   Size:        939     135
 
BstEII G'GTnAC_C

Cuts at:      0     667    1074 
   Size:        667     407
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     516    1074 
   Size:        516     558
 
BtgI C'CryG_G

Cuts at:      0      31     281     290     335    1058    1074 
   Size:         31     250       9      45     723      16

  Fragments arranged by size: 

                 723     250      45      31      16       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     864    1074 
   Size:        864     210
 
BtsI GCAGTG_nn'

Cuts at:      0     633    1074 
   Size:        633     441
 
EaeI y'GGCC_r

Cuts at:      0       8     209     233     278     401     466     524     602 
   Size:          8     201      24      45     123      65      58      78

Cuts at:    602     639    1074 
   Size:         37     435

  Fragments arranged by size: 

                 435     201     123      78      65      58      45      37
                  24       8
 
EagI C'GGCC_G

Cuts at:      0       8     209     278     401     466    1074 
   Size:          8     201      69     123      65     608

  Fragments arranged by size: 

                 608     201     123      69      65       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     538    1074 
   Size:        538     536
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      40    1015    1074 
   Size:         40     975      59

  Fragments arranged by size: 

                 975      59      40
 
EcoICRI GAG'CTC

Cuts at:      0    1043    1074 
   Size:       1043      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     964    1074 
   Size:        448     516     110

  Fragments arranged by size: 

                 516     448     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112     286     340     377     742    1074 
   Size:        112     174      54      37     365     332

  Fragments arranged by size: 

                 365     332     174     112      54      37
 
FseI GG_CCGG'CC

Cuts at:      0     530    1074 
   Size:        530     544
 
FspI TGC'GCA

Cuts at:      0     889     973    1074 
   Size:        889      84     101

  Fragments arranged by size: 

                 889     101      84
 
HaeII r_GCGC'y

Cuts at:      0      86     197     353     457     463     523     773    1074 
   Size:         86     111     156     104       6      60     250     301

  Fragments arranged by size: 

                 301     250     156     111     104      86      60       6
            
 
KasI G'GCGC_C

Cuts at:      0     349     453     769    1074 
   Size:        349     104     316     305

  Fragments arranged by size: 

                 349     316     305     104
 
MslI CAynn'nnrTG

Cuts at:      0     691     883    1074 
   Size:        691     192     191

  Fragments arranged by size: 

                 691     192     191
 
MspA1I CmG'CkG

Cuts at:      0      33     153     337     471     516     806     990    1032 
   Size:         33     120     184     134      45     290     184      42

Cuts at:   1032    1074 
   Size:         42

  Fragments arranged by size: 

                 290     184     184     134     120      45      42      42
                  33
 
NaeI GCC'GGC

Cuts at:      0     256     408     458     528     732    1074 
   Size:        256     152      50      70     204     342

  Fragments arranged by size: 

                 342     256     204     152      70      50
 
NarI GG'CG_CC

Cuts at:      0     350     454     770    1074 
   Size:        350     104     316     304

  Fragments arranged by size: 

                 350     316     304     104
 
NcoI C'CATG_G

Cuts at:      0    1058    1074 
   Size:       1058      16
 
NgoMIV G'CCGG_C

Cuts at:      0     254     406     456     526     730    1074 
   Size:        254     152      50      70     204     344

  Fragments arranged by size: 

                 344     254     204     152      70      50
 
NotI GC'GGCC_GC

Cuts at:      0     401     466    1074 
   Size:        401      65     608

  Fragments arranged by size: 

                 608     401      65
 
PspOMI G'GGCC_C

Cuts at:      0     111     285     339     376     741    1074 
   Size:        111     174      54      37     365     333

  Fragments arranged by size: 

                 365     333     174     111      54      37
 
PstI C_TGCA'G

Cuts at:      0      59     620     662    1074 
   Size:         59     561      42     412

  Fragments arranged by size: 

                 561     412      59      42
 
PvuII CAG'CTG

Cuts at:      0     516     990    1032    1074 
   Size:        516     474      42      42

  Fragments arranged by size: 

                 516     474      42      42
 
SacI G_AGCT'C

Cuts at:      0    1045    1074 
   Size:       1045      29
 
SacII CC_GC'GG

Cuts at:      0      34     338    1074 
   Size:         34     304     736

  Fragments arranged by size: 

                 736     304      34
 
SfcI C'TryA_G

Cuts at:      0      55     616     658    1074 
   Size:         55     561      42     416

  Fragments arranged by size: 

                 561     416      55      42
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     277     740    1074 
   Size:        277     463     334

  Fragments arranged by size: 

                 463     334     277
 
SfoI GGC'GCC

Cuts at:      0     351     455     771    1074 
   Size:        351     104     316     303

  Fragments arranged by size: 

                 351     316     303     104
 
SgrAI Cr'CCGG_yG

Cuts at:      0     456    1074 
   Size:        456     618
 
SmaI CCC'GGG

Cuts at:      0     103     326     363    1074 
   Size:        103     223      37     711

  Fragments arranged by size: 

                 711     223     103      37
 
SmlI C'TyrA_G

Cuts at:      0     757    1074 
   Size:        757     317
 
StyI C'CwwG_G

Cuts at:      0     272    1046    1058    1074 
   Size:        272     774      12      16

  Fragments arranged by size: 

                 774     272      16      12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     163     195    1074 
   Size:        163      32     879

  Fragments arranged by size: 

                 879     163      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279     828    1074 
   Size:        279     549     246

  Fragments arranged by size: 

                 549     279     246
 
XmaI C'CCGG_G

Cuts at:      0     101     324     361    1074 
   Size:        101     223      37     713

  Fragments arranged by size: 

                 713     223     101      37
 
ZraI GAC'GTC

Cuts at:      0     179    1074 
   Size:        179     895
 

 Enzymes that do cut:

     AarI    AatII     AleI     AlfI     ApaI     AscI     AvaI     BanI
    BanII     BbeI     BcgI     BclI     BglI     BlpI Bme1580I     BpmI
    BpuEI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI
     BtgI    BtgZI     BtsI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I     FseI     FspI    HaeII     KasI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NotI   PspOMI     PstI    PvuII
     SacI    SacII     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI
     StyI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI    BciVI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
     BsgI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrGI
    BstBI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI    EcoNI    EcoRI    EcoRV     FalI    FspAI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NdeI
     NheI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmnI
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