Restriction map for
pFN21AE1129
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI Tsp45I Tsp509I Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 19 GGCGCC G[GA]^CG[CT]C
AfaI 246 GTAC GT^AC
AflIII 367 ACATGT A^C[GA][CT]GT
Alw44I 254 GTGCAC G^TGCAC
ApaI 419 GGGCCC GGGCC^C
ApaLI 254 GTGCAC G^TGCAC
BbeI 19 GGCGCC GGCGC^C
BcnI 417 CCGGG CC^[GC]GG
BglI 283 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 19 GGCGCC G[GA]^CG[CT]C
BsaI 333 GGTCTC GGTCTC(1/5)
BsmAI 334 GTCTC GTCTC(1/5)
BspLU11I 367 ACATGT A^CATGT
BsrFI 431 GCCGGC [GA]^CCGG[CT]
BssSI 436 CACGAG CACGAG(-5/-1)
Cfr10I 431 GCCGGC [GA]^CCGG[CT]
DraII 419 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 257 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 281 TGGCCG [CT]^GGCC[GA]
EcoNI 453 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 419 GGGCCCT [GA]G^G[ACGT]CC[CT]
Hin1I 19 GGCGCC G[GA]^CG[CT]C
HinfI 163 GATTC G^A[ACGT]TC
HphI 317 TCACC GGTGA(8/7)
KasI 19 GGCGCC G^GCGCC
MseI 149 TTAA T^TAA
MspA1I 103 CCGCTG C[AC]G^C[GT]G
NaeI 431 GCCGGC GCC^GGC
NarI 19 GGCGCC GG^CGCC
NciI 417 CCGGG CC^[GC]GG
NcoI 186 CCATGG C^CATGG
NgoMIV 431 GCCGGC G^CCGGC
PflMI 78 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 246 GTAC GT^AC
SacI 40 GAGCTC GAGCT^C
SbfI 194 CCTGCAGG CCTGCA^GG
SfoI 19 GGCGCC GGC^GCC
SmlI 45 CTTGAG C^T[CT][GA]AG
SphI 267 GCATGC GCATG^C
Sse8387I 194 CCTGCAGG CCTGCA^GG
StuI 80 AGGCCT AGG^CCT
TaqI 26 TCGA T^CGA
TfiI 163 GATTC G^A[AT]TC
Tru9I 149 TTAA T^TAA
TthHB8I 26 TCGA T^CGA
Van91I 78 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AluI 41 AGCT AG^CT
49 AGCT
AspHI 40 GAGCTC G[AT]GC[AT]^C
254 GTGCAC
AvaII 273 GGACC G^G[AT]CC
393 GGTCC
BanI 19 GGCGCC G^G[CT][GA]CC
413 GGTGCC
BanII 40 GAGCTC G[GA]GC[CT]^C
419 GGGCCC
BsgI 110 GTGCAG GTGCAG(16/14)
208 GTGCAG
BsiHKAI 40 GAGCTC G[AT]GC[AT]^C
254 GTGCAC
BsmFI 99 GTCCC GGGAC(10/14)
141 GTCCC
BspMI 193 ACCTGC ACCTGC(4/8)
197 GCAGGT
CfoI 20 GCGC GCG^C
449 GCGC
DpnII 9 GATC ^GATC
28 GATC
FauI 102 CCCGC CCCGC(4/6)
117 CCCGC
HaeII 19 GGCGCC [GA]GCGC^[CT]
448 AGCGCC
HapII 417 CCGG C^CGG
432 CCGG
HhaI 20 GCGC GCG^C
449 GCGC
HinP1I 20 GCGC G^CGC
449 GCGC
HpaII 417 CCGG C^CGG
432 CCGG
MaeII 97 ACGT A^CGT
248 ACGT
MboI 9 GATC ^GATC
28 GATC
MboII 303 GAAGA GAAGA(8/7)
464 GAAGA
MslI 145 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
366 CACATGTGTG
MspI 417 CCGG C^CGG
432 CCGG
NdeII 9 GATC ^GATC
28 GATC
PstI 128 CTGCAG CTGCA^G
195 CTGCAG
Sau3AI 9 GATC ^GATC
28 GATC
SfcI 128 CTGCAG C^T[GA][CT]AG
195 CTGCAG
XcmI 314 CCATCACCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
338 CCATCGTGGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DsaI EcoRII EcoT14I
Fnu4HI HaeIII ItaI MnlI MvaI
MwoI NlaIII NlaIV NspI Sau96I
ScrFI StyI TseI TspRI