(Circular) (Six-base) MAPSORT of: pFN21AE1129.seq Check: 5373 from: 1 to: 5203 pFN21AE1129 5203 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3013 278 Size: 53 83 186 936 1477 2468 Fragments arranged by size: 2468 1477 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2546 1937 Size: 609 4594 Acc65I G'GTAC_C Cuts at: 2524 2524 Size: 5203 AclI AA'CG_TT Cuts at: 1563 3330 3703 1563 Size: 1767 373 3063 Fragments arranged by size: 3063 1767 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2483 3266 4650 4918 1632 Size: 851 783 1384 268 1917 Fragments arranged by size: 1917 1384 851 783 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5006 AflIII A'CryG_T Cuts at: 1203 2372 4102 5079 1203 Size: 1169 1730 977 1327 Fragments arranged by size: 1730 1327 1169 977 AgeI A'CCGG_T Cuts at: 4034 4034 Size: 5203 AhdI GACnn_n'nnGTC Cuts at: 3932 3932 Size: 5203 AleI CACnn'nnGTG Cuts at: 1166 2375 1166 Size: 1209 3994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2254 1184 Size: 1070 4133 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 2087 Size: 32 5171 AlwNI CAG_nnn'CTG Cuts at: 2946 4518 2946 Size: 1572 3631 ApaI G_GGCC'C Cuts at: 2428 2428 Size: 5203 ApaLI G'TGCA_C Cuts at: 2259 3260 4416 2259 Size: 1001 1156 3046 Fragments arranged by size: 3046 1156 1001 ApoI r'AATT_y Cuts at: 1493 2512 2738 2890 4999 1493 Size: 1019 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 226 152 AseI AT'TA_AT Cuts at: 160 1032 3757 160 Size: 872 2725 1606 Fragments arranged by size: 2725 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5203 AvaI C'yCGr_G Cuts at: 1870 1943 2528 1870 Size: 73 585 4545 Fragments arranged by size: 4545 585 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2242 2275 2242 Size: 33 5170 BamHI G'GATC_C Cuts at: 2533 2900 2533 Size: 367 4836 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 2418 2524 3979 618 Size: 325 217 627 237 394 106 1455 1842 Fragments arranged by size: 1842 1455 627 394 325 237 217 106 BanII G_rGCy'C Cuts at: 1391 2049 2428 2522 1391 Size: 658 379 94 4072 Fragments arranged by size: 4072 658 379 94 BbeI G_GCGC'C Cuts at: 2028 2028 Size: 5203 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3394 3428 1903 Size: 34 1457 34 3678 Fragments arranged by size: 3678 1457 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3095 4305 3095 Size: 1210 3993 BclI T'GATC_A Cuts at: 1548 1548 Size: 5203 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2294 3814 136 Size: 107 122 71 631 605 622 1520 1525 Fragments arranged by size: 1525 1520 631 622 605 122 107 71 BglII A'GATC_T Cuts at: 5198 5198 Size: 5203 BlpI GC'TnA_GC Cuts at: 2620 2620 Size: 5203 Bme1580I G_kGCm'C Cuts at: 1790 2263 2428 3264 4420 1790 Size: 473 165 836 1156 2573 Fragments arranged by size: 2573 1156 836 473 165 BmgBI CAC'GTC Cuts at: 1318 2103 1318 Size: 785 4418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2934 3892 442 Size: 416 53 83 399 24 1517 958 1753 Fragments arranged by size: 1753 1517 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5203 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2045 2045 Size: 5203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3863 1239 Size: 2624 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2071 3328 4193 4491 2071 Size: 1257 865 298 2783 Fragments arranged by size: 2783 1257 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2344 3866 882 Size: 617 845 1522 2219 Fragments arranged by size: 2219 1522 845 617 BsaAI yAC'GTr Cuts at: 493 1204 2994 493 Size: 711 1790 2702 Fragments arranged by size: 2702 1790 711 BsaBI GATnn'nnATC Cuts at: 5197 5197 Size: 5203 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2025 3010 3392 4027 Size: 53 83 186 936 492 985 382 635 Cuts at: 4027 275 Size: 1451 Fragments arranged by size: 1451 985 936 635 492 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3636 4034 4308 4455 4866 3636 Size: 398 274 147 411 3973 Fragments arranged by size: 3973 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4978 Fragments arranged by size: 4978 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5203 BseYI C'CCAG_C Cuts at: 2450 4406 4902 2450 Size: 1956 496 2751 Fragments arranged by size: 2751 1956 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2136 2234 1163 Size: 573 400 98 4132 Fragments arranged by size: 4132 573 400 98 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2653 3414 4442 1540 Size: 275 186 652 761 1028 2301 Fragments arranged by size: 2301 1028 761 652 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2263 3264 3349 4420 2049 Size: 214 1001 85 1071 2832 Fragments arranged by size: 2832 1071 1001 214 85 BsmI GAATG_Cn' Cuts at: 2714 2807 2714 Size: 93 5110 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2263 2428 2522 3264 3349 4420 Size: 399 259 214 165 94 742 85 1071 Cuts at: 4420 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 259 214 165 94 85 BspEI T'CCGG_A Cuts at: 4866 4866 Size: 5203 BspHI T'CATG_A Cuts at: 3092 4822 3092 Size: 1730 3473 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 2207 2558 844 Size: 1349 14 351 3489 Fragments arranged by size: 3489 1349 351 14 BsrBI CCG'CTC Cuts at: 1394 1823 3090 1394 Size: 429 1267 3507 Fragments arranged by size: 3507 1267 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3698 3872 65 Size: 1183 2450 174 1396 Fragments arranged by size: 2450 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2436 3847 4034 1700 Size: 110 626 1411 187 2869 Fragments arranged by size: 2869 1411 626 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5203 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5203 BssSI C'ACGA_G Cuts at: 2441 3263 4275 2441 Size: 822 1012 3369 Fragments arranged by size: 3369 1012 822 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5203 BstBI TT'CG_AA Cuts at: 4007 4007 Size: 5203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5203 BstYI r'GATC_y Cuts at: 1972 2533 2900 3285 3302 4754 4840 4852 5198 Size: 561 367 385 17 1452 86 12 346 Cuts at: 5198 1972 Size: 1977 Fragments arranged by size: 1977 1452 561 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2127 2191 2289 513 Size: 552 1062 64 98 3427 Fragments arranged by size: 3427 1062 552 98 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4464 Fragments arranged by size: 4464 683 56 BtsI GCAGTG_nn' Cuts at: 2145 2386 2719 3513 3533 2145 Size: 241 333 794 20 3815 Fragments arranged by size: 3815 794 333 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5203 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5168 DraI TTT'AAA Cuts at: 2507 2507 Size: 5203 DraIII CAC_nnn'GTG Cuts at: 2267 2267 Size: 5203 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4210 809 Size: 507 2894 1802 Fragments arranged by size: 2894 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2650 3539 Size: 54 1425 50 275 18 456 364 889 Cuts at: 3539 5168 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 456 364 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2650 1537 Size: 275 838 4090 Fragments arranged by size: 4090 838 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3133 1353 Size: 1780 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3790 4164 4310 387 Size: 1064 138 2201 374 146 1280 Fragments arranged by size: 2201 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2047 2047 Size: 5203 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2483 3266 3863 4650 4918 1239 Size: 393 851 783 597 787 268 1524 Fragments arranged by size: 1524 851 787 783 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 5203 EcoO109I rG'GnC_Cy Cuts at: 2425 2425 Size: 5203 EcoRI G'AATT_C Cuts at: 2512 2890 2512 Size: 378 4825 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2493 2493 Size: 5203 FspI TGC'GCA Cuts at: 3709 3709 Size: 5203 HaeII r_GCGC'y Cuts at: 1678 2028 2457 4350 1678 Size: 350 429 1893 2531 Fragments arranged by size: 2531 1893 429 350 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3844 3876 3918 3950 1373 Size: 32 135 32 2272 32 42 32 2626 Fragments arranged by size: 2626 2272 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2547 2793 669 Size: 988 281 609 246 3079 Fragments arranged by size: 3079 988 609 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5203 HpaI GTT'AAC Cuts at: 2793 2793 Size: 5203 KasI G'GCGC_C Cuts at: 2024 2024 Size: 5203 KpnI G_GTAC'C Cuts at: 2528 2528 Size: 5203 MfeI C'AATT_G Cuts at: 2802 2802 Size: 5203 MluI A'CGCG_T Cuts at: 5079 5079 Size: 5203 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4317 4501 1305 Size: 3012 184 2007 Fragments arranged by size: 3012 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3381 Fragments arranged by size: 3381 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2154 2375 3161 3520 3679 518 Size: 648 988 221 786 359 159 2042 Fragments arranged by size: 2042 988 786 648 359 221 159 MspA1I CmG'CkG Cuts at: 1606 2110 2619 3296 4444 4689 1606 Size: 504 509 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 509 504 245 NaeI GCC'GGC Cuts at: 2438 2438 Size: 5203 NarI GG'CG_CC Cuts at: 2025 2025 Size: 5203 NcoI C'CATG_G Cuts at: 513 1065 2191 513 Size: 552 1126 3525 Fragments arranged by size: 3525 1126 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5203 NgoMIV G'CCGG_C Cuts at: 2436 2436 Size: 5203 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5203 NotI GC'GGCC_GC Cuts at: 2650 2650 Size: 5203 NspI r_CATG'y Cuts at: 1132 1229 2235 2276 2376 2561 4106 1132 Size: 97 1006 41 100 185 1545 2229 Fragments arranged by size: 2229 1545 1006 185 100 97 41 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5203 PciI A'CATG_T Cuts at: 2372 4102 2372 Size: 1730 3473 PflMI CCAn_nnn'nTGG Cuts at: 1108 2089 1108 Size: 981 4222 PmeI GTTT'AAAC Cuts at: 2507 2507 Size: 5203 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3272 3304 4785 4817 3272 Size: 32 1481 32 3658 Fragments arranged by size: 3658 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5203 PsiI TTA'TAA Cuts at: 2773 2773 Size: 5203 PspOMI G'GGCC_C Cuts at: 2424 2424 Size: 5203 PstI C_TGCA'G Cuts at: 830 2137 2204 2555 830 Size: 1307 67 351 3478 Fragments arranged by size: 3478 1307 351 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5203 SacI G_AGCT'C Cuts at: 2049 2049 Size: 5203 SalI G'TCGA_C Cuts at: 1936 2545 1936 Size: 609 4594 SbfI CC_TGCA'GG Cuts at: 2204 2555 2204 Size: 351 4852 ScaI AGT'ACT Cuts at: 3451 5163 3451 Size: 1712 3491 SfcI C'TryA_G Cuts at: 826 1045 2133 2200 2551 3686 4367 4558 826 Size: 219 1088 67 351 1135 681 191 1471 Fragments arranged by size: 1471 1135 1088 681 351 219 191 67 SfoI GGC'GCC Cuts at: 2026 2026 Size: 5203 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5203 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5203 SmaI CCC'GGG Cuts at: 1872 2530 1872 Size: 658 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2050 3307 4208 4470 820 Size: 197 926 107 1257 901 262 1553 Fragments arranged by size: 1553 1257 926 901 262 197 107 SnaBI TAC'GTA Cuts at: 493 2994 493 Size: 2501 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5203 SphI G_CATG'C Cuts at: 1132 2276 2561 1132 Size: 1144 285 3774 Fragments arranged by size: 3774 1144 285 SspI AAT'ATT Cuts at: 5 52 3127 5 Size: 47 3075 2081 Fragments arranged by size: 3075 2081 47 StuI AGG'CCT Cuts at: 1517 2087 1517 Size: 570 4633 StyI C'CwwG_G Cuts at: 513 1065 2088 2191 2328 2642 513 Size: 552 1023 103 137 314 3074 Fragments arranged by size: 3074 1023 552 314 137 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3230 3247 3400 3585 4030 325 Size: 1255 1650 17 153 185 445 1498 Fragments arranged by size: 1650 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3449 5161 96 Size: 274 80 33 51 1443 1472 1712 138 Fragments arranged by size: 1712 1472 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5163 5195 5163 Size: 32 5171 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5203 XbaI T'CTAG_A Cuts at: 2539 2539 Size: 5203 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2350 2326 Size: 24 5179 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5203 XmaI C'CCGG_G Cuts at: 1870 2528 1870 Size: 658 4545 XmnI GAAnn'nnTTC Cuts at: 3332 3332 Size: 5203 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3011 276 Size: 53 83 186 936 1477 2468 Fragments arranged by size: 2468 1477 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI