################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraII DraIII DrdI EarI Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MboI MfeI MflI MluI MroI MseI MunI NarI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AhdI 30 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 361 AGCGCT AGC^GCT AspEI 30 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 147 CCCGGG C^[CT]CG[GA]G BalI 413 TGGCCA TGG^CCA BanI 223 GGCACC G^G[CT][GA]CC BbsI 478 GAAGAC GAAGAC(2/6) BciVI 294 GTATCC GTATCC(6/5) BglI 433 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 357 GCTGAGC GC^T[ACGT]AGC Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC BpuAI 478 GAAGAC GAAGAC(2/6) BsaI 37 GGTCTC GGTCTC(1/5) BsiEI 70 CGGCCG CG[GA][CT]^CG BsoBI 147 CCCGGG C^[CT]CG[GA]G BspHI 207 TCATGA T^CATGA BssHII 16 GCGCGC G^CGCGC BstF5I 266 CATCC GGATG(2/0) CelII 357 GCTGAGC GC^T[ACGT]AGC EagI 70 CGGCCG C^GGCCG Eam1105I 30 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 70 CGGCCG C^GGCCG Eco47III 361 AGCGCT AGC^GCT Eco52I 70 CGGCCG C^GGCCG EcoNI 178 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 434 CCATGG C^C[AT][AT]GG FokI 266 CATCC GGATG(9/13) HgaI 81 GACGC GACGC(5/10) HinfI 211 GAGTC G^A[ACGT]TC KspI 7 CCGCGG CCGC^GG MaeII 334 ACGT A^CGT MaeIII 451 GTGAC ^GT[ACGT]AC MboII 478 GAAGA GAAGA(8/7) MluNI 413 TGGCCA TGG^CCA MscI 413 TGGCCA TGG^CCA MslI 315 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 75 GCCGGC GCC^GGC NcoI 434 CCATGG C^CATGG NgoMIV 75 GCCGGC G^CCGGC NspI 379 GCATGC [GA]CATG^[CT] PflMI 245 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 211 GAGTC GAGTC(4/5) PvuII 157 CAGCTG CAG^CTG RcaI 207 TCATGA T^CATGA SacII 7 CCGCGG CCGC^GG SgrAI 74 CGCCGGCG C[GA]^CCGG[CT]G SmaI 147 CCCGGG CCC^GGG SmlI 388 CTCAAG C^T[CT][GA]AG SphI 379 GCATGC GCATG^C StyI 434 CCATGG C^C[AT][AT]GG Tsp45I 451 GTGAC ^GT[GC]AC Van91I 245 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 416 CCAGCGTTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 147 CCCGGG C^CCGGG XmaI 147 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AvaII 29 GGACC G^G[AT]CC 461 GGACC BanII 88 GAGCCC G[GA]GC[CT]^C 150 GGGCTC BmyI 88 GAGCCC G[AGT]GC[ACT]^C 150 GGGCTC BsmAI 38 GTCTC GTCTC(1/5) 369 GTCTC BsmFI 460 GGGAC GGGAC(10/14) 145 GTCCC Bsp1286I 88 GAGCCC G[AGT]GC[ACT]^C 150 GGGCTC BsrFI 75 GCCGGC [GA]^CCGG[CT] 447 GCCGGT Cfr10I 75 GCCGGC [GA]^CCGG[CT] 447 GCCGGT DdeI 233 CTCAG C^T[ACGT]AG 358 CTGAG HaeII 45 GGCGCT [GA]GCGC^[CT] 361 AGCGCT SfaNI 162 GATGC GCATC(5/9) 265 GCATC TspRI 318 CACTG CA[GC]TG[ACGT][ACGT]^ 339 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BsaJI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI