################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII AccIII AclI AflII AflIII AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbrPI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BsrI Bst1107I BstAPI BstBI BstF5I BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfcI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 244 GGTACC G^GTACC AccI 673 GTCTAC GT^[AC][GT]AC AcsI 1147 GAATTT [GA]^AATT[CT] AgeI 241 ACCGGT A^CCGGT Aor51HI 386 AGCGCT AGC^GCT ApaI 25 GGGCCC GGGCC^C ApoI 1147 GAATTT [GA]^AATT[CT] Asp718I 244 GGTACC G^GTACC AspHI 943 GTGCTC G[AT]GC[AT]^C AspI 983 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 562 GGCGCC GGCGC^C BbsI 787 GTCTTC GAAGAC(2/6) BcgI 1065 CGATTCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 733 GCTCAGC GC^T[ACGT]AGC Bpu1102I 733 GCTCAGC GC^T[ACGT]AGC BpuAI 787 GTCTTC GAAGAC(2/6) BsaWI 241 ACCGGT [AT]^CCGG[AT] BsiHKAI 943 GTGCTC G[AT]GC[AT]^C BspHI 332 TCATGA T^CATGA BspMI 125 ACCTGC ACCTGC(4/8) BsrFI 241 ACCGGT [GA]^CCGG[CT] BssHII 152 GCGCGC G^CGCGC BssSI 1070 CTCGTG CACGAG(-5/-1) BstEII 237 GGTGACC G^GT[ACGT]ACC BstPI 237 GGTGACC G^GT[ACGT]ACC BstXI 122 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 902 AGATCC [GA]^GATC[CT] CelII 733 GCTCAGC GC^T[ACGT]AGC Cfr10I 241 ACCGGT [GA]^CCGG[CT] EagI 473 CGGCCG C^GGCCG EclXI 473 CGGCCG C^GGCCG Eco47III 386 AGCGCT AGC^GCT Eco52I 473 CGGCCG C^GGCCG EcoNI 1189 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 237 GGTGACC G^GT[ACGT]ACC EcoT14I 374 CCATGG C^C[AT][AT]GG KasI 562 GGCGCC G^GCGCC KpnI 244 GGTACC GGTAC^C MaeIII 238 GTGAC ^GT[ACGT]AC MflI 902 AGATCC [GA]^GATC[CT] NarI 562 GGCGCC GG^CGCC NcoI 374 CCATGG C^CATGG NotI 472 GCGGCCGC GC^GGCCGC NspI 1130 GCATGC [GA]CATG^[CT] PflFI 983 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 241 ACCGGT A^CCGGT PpuMI 538 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 332 TCATGA T^CATGA SfiI 369 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 562 GGCGCC GGC^GCC SphI 1130 GCATGC GCATG^C StyI 374 CCATGG C^C[AT][AT]GG TaqI 278 TCGA T^CGA TfiI 1066 GATTC G^A[AT]TC Tsp45I 238 GTGAC ^GT[GC]AC Tsp509I 1148 AATT ^AATT Tth111I 983 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 278 TCGA T^CGA Van91I 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 452 CCACCGTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 902 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwI 903 GATCC GGATC(4/5) 746 GGATC AlwNI 736 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 901 CAGATCCTG BanI 244 GGTACC G^G[CT][GA]CC 562 GGCGCC BanII 25 GGGCCC G[GA]GC[CT]^C 828 GAGCCC BbvCI 966 CCTCAGC CCTCAGC(-5/-2) 1076 CCTCAGC BglI 370 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 475 GCCGCGCTGGC BseRI 180 CTCCTC GAGGAG(10/8) 58 GAGGAG EarI 274 CTCTTC CTCTTC(1/4) 595 CTCTTC Ksp632I 274 CTCTTC CTCTTC(1/4) 595 CTCTTC MaeII 578 ACGT A^CGT 671 ACGT MslI 249 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 750 CATGGTGGTG PvuII 841 CAGCTG CAG^CTG 1091 CAGCTG SapI 273 GCTCTTC GCTCTTC(1/4) 594 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BalI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiEI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SmaI TseI TspRI XmaCI XmaI