################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1321 GTCTAC GT^[AC][GT]AC AcsI 1236 AAATTT [GA]^AATT[CT] AfaI 419 GTAC GT^AC ApoI 1236 AAATTT [GA]^AATT[CT] AseI 190 ATTAAT AT^TAAT AsnI 190 ATTAAT AT^TAAT BanII 281 GAGCCC G[GA]GC[CT]^C BbsI 1452 GAAGAC GAAGAC(2/6) BcgI 51 CGACTTAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 284 CCCGG CC^[GC]GG BfaI 1489 CTAG C^TAG BglI 1466 GCCACGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1421 AGATCT A^GATCT BpmI 203 CTGGAG CTGGAG(16/14) BpuAI 1452 GAAGAC GAAGAC(2/6) BseRI 1063 GAGGAG GAGGAG(10/8) BsgI 637 CTGCAC GTGCAG(16/14) BsiEI 65 CGGTCG CG[GA][CT]^CG BspHI 498 TCATGA T^CATGA BsrFI 1267 ACCGGC [GA]^CCGG[CT] Cfr10I 1267 ACCGGC [GA]^CCGG[CT] DrdI 310 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1071 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1154 ATGCAT ATGCA^T HaeII 1037 GGCGCT [GA]GCGC^[CT] HincII 877 GTCAAC GT[CT]^[GA]AC HindII 877 GTCAAC GT[CT]^[GA]AC HphI 1276 GGTGA GGTGA(8/7) MaeI 1489 CTAG C^TAG MfeI 830 CAATTG C^AATTG MunI 830 CAATTG C^AATTG NciI 284 CCCGG CC^[GC]GG NcoI 1377 CCATGG C^CATGG NdeI 1529 CATATG CA^TATG NruI 10 TCGCGA TCG^CGA NsiI 1154 ATGCAT ATGCA^T PshAI 1353 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 190 ATTAAT AT^TAAT RcaI 498 TCATGA T^CATGA RsaI 419 GTAC GT^AC SapI 223 GAAGAGC GCTCTTC(1/4) SexAI 490 ACCTGGT A^CC[AT]GGT SmlI 1058 CTCAAG C^T[CT][GA]AG SspI 187 AATATT AAT^ATT TfiI 600 GATTC G^A[AT]TC XcmI 851 CCACTGTCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 11 CGCG CG^CG 977 CGCG AlwI 1068 GATCC GGATC(4/5) 173 GGATC BanI 1012 GGCACC G^G[CT][GA]CC 1399 GGTGCC BbvI 1509 GCAGC GCAGC(8/12) 636 GCTGC BmyI 281 GAGCCC G[AGT]GC[ACT]^C 1011 GGGCAC BsaWI 63 TCCGGT [AT]^CCGG[AT] 650 TCCGGT Bsp1286I 281 GAGCCC G[AGT]GC[ACT]^C 1011 GGGCAC BspMI 1018 ACCTGC ACCTGC(4/8) 1285 ACCTGC BstAPI 630 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1013 GCACCACCTGC BstUI 11 CGCG CG^CG 977 CGCG BstXI 725 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1116 CCATCAAGATGG BstYI 1067 AGATCC [GA]^GATC[CT] 1421 AGATCT CfoI 976 GCGC GCG^C 1038 GCGC DsaI 23 CCACGG C^C[GA][CT]GG 1377 CCATGG FauI 1446 CCCGC CCCGC(4/6) 978 GCGGG HhaI 976 GCGC GCG^C 1038 GCGC HinP1I 976 GCGC G^CGC 1038 GCGC HindIII 323 AAGCTT A^AGCTT 913 AAGCTT MaeII 1002 ACGT A^CGT 1188 ACGT MflI 1067 AGATCC [GA]^GATC[CT] 1421 AGATCT MslI 1092 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1117 CATCAAGATG MspA1I 1309 CAGCGG C[AC]G^C[GT]G 1447 CCGCTG MvnI 11 CGCG CG^CG 977 CGCG PflMI 1086 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1418 CCAAGATCTGG TseI 636 GCTGC G^C[AT]GC 1509 GCAGC Van91I 1086 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1418 CCAAGATCTGG XhoII 1067 AGATCC [GA]^GATC[CT] 1421 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AvaII BalI BsaJI BsiYI BslI BsmAI BsmFI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I