################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AccII AccIII AclI AgeI AhdI AocI ApaI AscI AseI AsnI AspEI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BsrDI BssHII Bst1107I BstBI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco81I EcoRV FbaI FseI FspI HindIII HpaI KasI KspI MaeI MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI TthHB8I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AatII 139 GACGTC GACGT^C Acc65I 1351 GGTACC G^GTACC AccI 937 GTAGAC GT^[AC][GT]AC AcyI 139 GACGTC G[GA]^CG[CT]C AflII 1330 CTTAAG C^TTAAG AflIII 1688 ACGTGT A^C[GA][CT]GT Alw44I 480 GTGCAC G^TGCAC Aor51HI 671 AGCGCT AGC^GCT ApaLI 480 GTGCAC G^TGCAC Asp700I 955 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1351 GGTACC G^GTACC AspHI 480 GTGCAC G[AT]GC[AT]^C AspI 88 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 323 CCCGGG C^[CT]CG[GA]G BanI 1351 GGTACC G^G[CT][GA]CC BciVI 1633 GGATAC GTATCC(6/5) BfrI 1330 CTTAAG C^TTAAG BsaAI 1621 TACGTG [CT]AC^GT[GA] BsaHI 139 GACGTC G[GA]^CG[CT]C BsaI 1059 GGTCTC GGTCTC(1/5) BsiEI 1190 CGGCCG CG[GA][CT]^CG BsiHKAI 480 GTGCAC G[AT]GC[AT]^C BsmBI 127 CGTCTC CGTCTC(1/5) BsmI 1643 GCATTC GAATGC(1/-1) BsoBI 323 CCCGGG C^[CT]CG[GA]G Bsp1407I 1025 TGTACA T^GTACA BspHI 1365 TCATGA T^CATGA BsrFI 1422 ACCGGC [GA]^CCGG[CT] BsrGI 1025 TGTACA T^GTACA BssSI 1093 CACGAG CACGAG(-5/-1) BstAPI 1583 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 846 GGTAACC G^GT[ACGT]ACC BstPI 846 GGTAACC G^GT[ACGT]ACC Cfr10I 1422 ACCGGC [GA]^CCGG[CT] DraII 163 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 250 GACAGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1190 CGGCCG C^GGCCG EclXI 1190 CGGCCG C^GGCCG Eco47III 671 AGCGCT AGC^GCT Eco52I 1190 CGGCCG C^GGCCG EcoNI 1089 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 163 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 846 GGTAACC G^GT[ACGT]ACC EcoRI 1504 GAATTC G^AATTC EcoT22I 401 ATGCAT ATGCA^T FauI 1041 GCGGG CCCGC(4/6) HaeII 671 AGCGCT [GA]GCGC^[CT] Hin1I 139 GACGTC G[GA]^CG[CT]C HincII 259 GTCAAC GT[CT]^[GA]AC HindII 259 GTCAAC GT[CT]^[GA]AC KpnI 1351 GGTACC GGTAC^C NsiI 401 ATGCAT ATGCA^T PflFI 88 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 634 GAGTC GAGTC(4/5) PpuMI 163 AGGACCT [GA]G^G[AT]CC[CT] PstI 829 CTGCAG CTGCA^G RcaI 1365 TCATGA T^CATGA SapI 598 GAAGAGC GCTCTTC(1/4) SfcI 829 CTGCAG C^T[GA][CT]AG SmaI 323 CCCGGG CCC^GGG SmlI 1330 CTTAAG C^T[CT][GA]AG SspBI 1025 TGTACA T^GTACA Tth111I 88 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 323 CCCGGG C^CCGGG XmaI 323 CCCGGG C^CCGGG XmnI 955 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 1504 GAATTC [GA]^AATT[CT] 1528 AAATTC AlwI 1675 GATCC GGATC(4/5) 650 GGATC ApoI 1504 GAATTC [GA]^AATT[CT] 1528 AAATTC BseRI 318 CTCCTC GAGGAG(10/8) 1096 GAGGAG BsgI 1090 CTGCAC GTGCAG(16/14) 407 GTGCAG BsmFI 1433 GTCCC GGGAC(10/14) 1711 GTCCC BspMI 428 ACCTGC ACCTGC(4/8) 1236 GCAGGT BstXI 440 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1118 CCATGCTGGTGG CfoI 672 GCGC GCG^C 1581 GCGC DdeI 517 CTCAG C^T[ACGT]AG 1221 CTCAG HgaI 92 GCGTC GACGC(5/10) 126 GCGTC HhaI 672 GCGC GCG^C 1581 GCGC HinP1I 672 GCGC G^CGC 1581 GCGC PflMI 567 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1593 CCATGCTGTGG PvuII 559 CAGCTG CAG^CTG 1460 CAGCTG SphI 196 GCATGC GCATG^C 452 GCATGC Van91I 567 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1593 CCATGCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BalI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI