(Circular) (Six-base) MAPSORT of: pFN21AB9591.seq Check: 2260 from: 1 to: 6472 pFN21AB9591 6472 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2148 4282 278 Size: 53 83 186 936 612 2134 2468 Fragments arranged by size: 2468 2134 936 612 186 83 53 AccI GT'mk_AC Cuts at: 1937 2943 3815 1937 Size: 1006 872 4594 Fragments arranged by size: 4594 1006 872 Acc65I G'GTAC_C Cuts at: 3356 3793 3356 Size: 437 6035 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 3182 4535 5919 6187 1632 Size: 1058 492 1353 1384 268 1917 Fragments arranged by size: 1917 1384 1353 1058 492 268 AfeI AGC'GCT Cuts at: 2678 2678 Size: 6472 AflII C'TTAA_G Cuts at: 820 1017 3335 820 Size: 197 2318 3957 Fragments arranged by size: 3957 2318 197 AflIII A'CryG_T Cuts at: 1203 3693 5371 6348 1203 Size: 2490 1678 977 1327 Fragments arranged by size: 2490 1678 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 2482 1166 Size: 1316 5156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2480 2500 3223 1184 Size: 1296 20 723 4433 Fragments arranged by size: 4433 1296 723 20 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 2018 Size: 32 6440 AlwNI CAG_nnn'CTG Cuts at: 2862 3467 3753 4215 5787 2862 Size: 605 286 462 1572 3547 Fragments arranged by size: 3547 1572 605 462 286 ApaLI G'TGCA_C Cuts at: 2485 4529 5685 2485 Size: 2044 1156 3272 Fragments arranged by size: 3272 2044 1156 ApoI r'AATT_y Cuts at: 1493 3509 3533 3781 4007 4159 6268 1493 Size: 2016 24 248 226 152 2109 1697 Fragments arranged by size: 2109 2016 1697 248 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2328 3797 1870 Size: 73 385 1469 4545 Fragments arranged by size: 4545 1469 385 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3348 3381 3348 Size: 33 6439 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3356 3793 5248 618 Size: 325 217 627 1569 437 1455 1842 Fragments arranged by size: 1842 1569 1455 627 437 325 217 BanII G_rGCy'C Cuts at: 1391 3791 1391 Size: 2400 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4364 5574 3632 Size: 732 1210 4530 Fragments arranged by size: 4530 1210 732 BclI T'GATC_A Cuts at: 1548 1548 Size: 6472 BfrBI ATG'CAT Cuts at: 2408 2408 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5083 136 Size: 107 122 71 631 605 3411 1525 Fragments arranged by size: 3411 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 2489 2869 3192 4533 5689 1790 Size: 699 380 323 1341 1156 2573 Fragments arranged by size: 2573 1341 1156 699 380 323 BmgBI CAC'GTC Cuts at: 1318 3694 1318 Size: 2376 4096 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3048 4203 5161 Size: 416 53 83 399 24 1631 1155 958 Cuts at: 5161 442 Size: 1753 Fragments arranged by size: 1753 1631 1155 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2546 2630 5132 1239 Size: 1166 141 84 2502 2579 Fragments arranged by size: 2579 2502 1166 141 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4597 5462 5760 4597 Size: 865 298 5309 Fragments arranged by size: 5309 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3070 5135 882 Size: 617 1571 2065 2219 Fragments arranged by size: 2219 2065 1571 617 BsaAI yAC'GTr Cuts at: 493 1204 3628 4263 493 Size: 711 2424 635 2702 Fragments arranged by size: 2702 2424 711 635 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 4279 4661 5296 Size: 53 83 186 936 612 2134 382 635 Cuts at: 5296 275 Size: 1451 Fragments arranged by size: 2134 1451 936 635 612 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 2503 2533 1178 Size: 30 165 30 663 30 407 30 5117 Fragments arranged by size: 5117 663 407 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 3116 1186 Size: 1128 802 4542 Fragments arranged by size: 4542 1128 802 BseYI C'CCAG_C Cuts at: 2984 3441 3472 5675 6171 2984 Size: 457 31 2203 496 3285 Fragments arranged by size: 3285 2203 496 457 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2433 3080 1163 Size: 573 697 647 4555 Fragments arranged by size: 4555 697 647 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3198 3922 4683 5711 1540 Size: 275 186 1197 724 761 1028 2301 Fragments arranged by size: 2301 1197 1028 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 2489 4533 4618 5689 2489 Size: 2044 85 1071 3272 Fragments arranged by size: 3272 2044 1071 85 BsmI GAATG_Cn' Cuts at: 3648 3983 4076 3648 Size: 335 93 6044 Fragments arranged by size: 6044 335 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2138 2138 Size: 6472 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2489 2869 3192 3791 4533 4618 5689 Size: 399 699 380 323 599 742 85 1071 Cuts at: 5689 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 699 599 399 380 323 85 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 3370 4361 6091 3370 Size: 991 1730 3751 Fragments arranged by size: 3751 1730 991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 3232 3827 844 Size: 1598 790 595 3489 Fragments arranged by size: 3489 1598 790 595 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4967 5141 65 Size: 1183 3719 174 1396 Fragments arranged by size: 3719 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3427 5116 5303 1700 Size: 110 1617 1689 187 2869 Fragments arranged by size: 2869 1689 1617 187 110 BsrGI T'GTAC_A Cuts at: 96 3030 96 Size: 2934 3538 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 3098 4532 5544 3098 Size: 1434 1012 4026 Fragments arranged by size: 4026 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3594 794 Size: 2800 3672 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 2851 1184 Size: 1667 4805 BstXI CCAn_nnnn'nTGG Cuts at: 2452 3130 2452 Size: 678 5794 BstYI r'GATC_y Cuts at: 1972 3802 4169 4554 4571 6023 6109 6121 6467 Size: 1830 367 385 17 1452 86 12 346 Cuts at: 6467 1972 Size: 1977 Fragments arranged by size: 1977 1830 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2757 3145 3279 513 Size: 552 1692 388 134 3706 Fragments arranged by size: 3706 1692 552 388 134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3670 487 Size: 683 56 2444 3289 Fragments arranged by size: 3289 2444 683 56 BtsI GCAGTG_nn' Cuts at: 2196 3166 3988 4782 4802 2196 Size: 970 822 794 20 3866 Fragments arranged by size: 3866 970 822 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2261 5479 809 Size: 507 945 3218 1802 Fragments arranged by size: 3218 1802 945 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2754 2884 3132 Size: 54 1425 50 275 18 924 130 248 Cuts at: 3132 3142 3195 3429 3919 4808 6437 8 Size: 10 53 234 490 889 1629 43 Fragments arranged by size: 1629 1425 924 889 490 275 248 234 130 54 53 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 3195 3919 1537 Size: 275 1383 724 4090 Fragments arranged by size: 4090 1383 724 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 2598 2799 2900 4402 1353 Size: 788 457 201 101 1502 3423 Fragments arranged by size: 3423 1502 788 457 201 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5059 5433 5579 387 Size: 1064 138 3470 374 146 1280 Fragments arranged by size: 3470 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2546 2630 2690 3182 4535 5132 Size: 393 773 141 84 60 492 1353 597 Cuts at: 5132 5919 6187 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1353 787 773 597 492 393 268 141 84 60 EcoNI CCTnn'n_nnAGG Cuts at: 3098 3098 Size: 6472 EcoO109I rG'GnC_Cy Cuts at: 2169 2169 Size: 6472 EcoRI G'AATT_C Cuts at: 3509 3781 4159 3509 Size: 272 378 5822 Fragments arranged by size: 5822 378 272 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 2680 5619 1678 Size: 1002 2939 2531 Fragments arranged by size: 2939 2531 1002 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2503 2535 3092 3124 5113 Size: 32 135 32 931 32 557 32 1989 Cuts at: 5113 5145 5187 5219 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1989 931 557 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 3816 4062 669 Size: 988 281 328 1550 246 3079 Fragments arranged by size: 3079 1550 988 328 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 3360 3797 3360 Size: 437 6035 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3613 3707 5586 5770 1305 Size: 2308 94 1879 184 2007 Fragments arranged by size: 2308 2007 1879 184 94 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 2886 3134 3144 10 Size: 54 1425 343 924 130 248 10 3338 Fragments arranged by size: 3338 1425 924 343 248 130 54 10 MslI CAynn'nnrTG Cuts at: 518 1166 2482 2582 3128 4430 4789 4948 518 Size: 648 1316 100 546 1302 359 159 2042 Fragments arranged by size: 2042 1316 1302 648 546 359 159 100 MspA1I CmG'CkG Cuts at: 1606 2049 2566 3467 3888 4565 5713 5958 1606 Size: 443 517 901 421 677 1148 245 2120 Fragments arranged by size: 2120 1148 901 677 517 443 421 245 NcoI C'CATG_G Cuts at: 513 1065 2757 3145 3279 513 Size: 552 1692 388 134 3706 Fragments arranged by size: 3706 1692 552 388 134 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NsiI A_TGCA'T Cuts at: 2410 2410 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 2205 2383 2412 2461 3012 3830 5375 Size: 97 976 178 29 49 551 818 1545 Cuts at: 5375 1132 Size: 2229 Fragments arranged by size: 2229 1545 976 818 551 178 97 49 29 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6472 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2578 3604 1108 Size: 1470 1026 3976 Fragments arranged by size: 3976 1470 1026 PfoI T'CCnGG_A Cuts at: 2364 2400 2421 2364 Size: 36 21 6415 Fragments arranged by size: 6415 36 21 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2210 2242 4541 4573 6054 6086 2210 Size: 32 2299 32 1481 32 2596 Fragments arranged by size: 2596 2299 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2169 2169 Size: 6472 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 3531 4042 3531 Size: 511 5961 PstI C_TGCA'G Cuts at: 830 2838 3824 830 Size: 2008 986 3478 Fragments arranged by size: 3478 2008 986 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2566 3467 2566 Size: 901 5571 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SapI GCTCTTCn'nnn_ Cuts at: 2598 2598 Size: 6472 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2834 3820 4955 5636 5827 826 Size: 219 1789 986 1135 681 191 1471 Fragments arranged by size: 1789 1471 1135 986 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 2330 3799 1872 Size: 458 1469 4545 Fragments arranged by size: 4545 1469 458 SmlI C'TyrA_G Cuts at: 820 1017 1943 3335 4576 5477 5739 820 Size: 197 926 1392 1241 901 262 1553 Fragments arranged by size: 1553 1392 1241 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 2205 2461 3830 1132 Size: 1073 256 1369 3774 Fragments arranged by size: 3774 1369 1073 256 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2757 2970 3111 3145 3279 3561 3911 Size: 552 1692 213 141 34 134 282 350 Cuts at: 3911 513 Size: 3074 Fragments arranged by size: 3074 1692 552 350 282 213 141 134 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2301 4499 4516 4669 4854 5299 325 Size: 1255 721 2198 17 153 185 445 1498 Fragments arranged by size: 2198 1498 1255 721 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3030 4718 6430 Size: 274 80 33 51 1443 1053 1688 1712 Cuts at: 6430 96 Size: 138 Fragments arranged by size: 1712 1688 1443 1053 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2066 2098 6432 6464 2066 Size: 32 4334 32 2074 Fragments arranged by size: 4334 2074 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2096 1316 Size: 780 5692 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 2605 2740 2449 Size: 156 135 6181 Fragments arranged by size: 6181 156 135 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 2328 3797 1870 Size: 458 1469 4545 Fragments arranged by size: 4545 1469 458 XmnI GAAnn'nnTTC Cuts at: 2964 4601 2964 Size: 1637 4835 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2146 4280 276 Size: 53 83 186 936 612 2134 2468 Fragments arranged by size: 2468 2134 936 612 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI