(Linear) (Six-base) MAPSORT of: KIBB9591.seq Check: 20 from: 1 to: 1767 KIBB9591.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AatII G_ACGT'C Cuts at: 0 143 1767 Size: 143 1624 AccI GT'mk_AC Cuts at: 0 938 1767 Size: 938 829 Acc65I G'GTAC_C Cuts at: 0 1351 1767 Size: 1351 416 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 1177 1767 Size: 685 492 590 Fragments arranged by size: 685 590 492 AfeI AGC'GCT Cuts at: 0 673 1767 Size: 673 1094 AflII C'TTAA_G Cuts at: 0 1330 1767 Size: 1330 437 AflIII A'CryG_T Cuts at: 0 1688 1767 Size: 1688 79 AleI CACnn'nnGTG Cuts at: 0 477 1767 Size: 477 1290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 475 495 1218 1767 Size: 475 20 723 549 Fragments arranged by size: 723 549 475 20 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 13 45 1767 Size: 13 32 1722 Fragments arranged by size: 1722 32 13 AlwNI CAG_nnn'CTG Cuts at: 0 857 1462 1748 1767 Size: 857 605 286 19 Fragments arranged by size: 857 605 286 19 ApaLI G'TGCA_C Cuts at: 0 480 1767 Size: 480 1287 ApoI r'AATT_y Cuts at: 0 1504 1528 1767 Size: 1504 24 239 Fragments arranged by size: 1504 239 24 AvaI C'yCGr_G Cuts at: 0 323 1767 Size: 323 1444 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1343 1376 1767 Size: 1343 33 391 Fragments arranged by size: 1343 391 33 BanI G'GyrC_C Cuts at: 0 1351 1767 Size: 1351 416 BciVI GTATCCnnnnn_n' Cuts at: 0 1627 1767 Size: 1627 140 BfrBI ATG'CAT Cuts at: 0 403 1767 Size: 403 1364 Bme1580I G_kGCm'C Cuts at: 0 484 864 1187 1767 Size: 484 380 323 580 Fragments arranged by size: 580 484 380 323 BmgBI CAC'GTC Cuts at: 0 1689 1767 Size: 1689 78 BmrI ACTGGGnnnn_n' Cuts at: 0 1043 1767 Size: 1043 724 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 541 625 1767 Size: 400 141 84 1142 Fragments arranged by size: 1142 400 141 84 BsaI GGTCTCn'nnnn_ Cuts at: 0 1065 1767 Size: 1065 702 BsaAI yAC'GTr Cuts at: 0 1623 1767 Size: 1623 144 BsaHI Gr'CG_yC Cuts at: 0 140 1767 Size: 140 1627 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 61 91 498 528 1767 Size: 61 30 407 30 1239 Fragments arranged by size: 1239 407 61 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 309 1111 1767 Size: 309 802 656 Fragments arranged by size: 802 656 309 BseYI C'CCAG_C Cuts at: 0 979 1436 1467 1767 Size: 979 457 31 300 Fragments arranged by size: 979 457 300 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 428 1075 1767 Size: 428 647 692 Fragments arranged by size: 692 647 428 BsiEI CG_ry'CG Cuts at: 0 1193 1767 Size: 1193 574 BsiHKAI G_wGCw'C Cuts at: 0 484 1767 Size: 484 1283 BsmI GAATG_Cn' Cuts at: 0 1643 1767 Size: 1643 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 133 1767 Size: 133 1634 Bsp1286I G_dGCh'C Cuts at: 0 484 864 1187 1767 Size: 484 380 323 580 Fragments arranged by size: 580 484 380 323 BspHI T'CATG_A Cuts at: 0 1365 1767 Size: 1365 402 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 437 1227 1767 Size: 437 790 540 Fragments arranged by size: 790 540 437 BsrFI r'CCGG_y Cuts at: 0 1422 1767 Size: 1422 345 BsrGI T'GTAC_A Cuts at: 0 1025 1767 Size: 1025 742 BssSI C'ACGA_G Cuts at: 0 1093 1767 Size: 1093 674 BstAPI GCAn_nnn'nTGC Cuts at: 0 1589 1767 Size: 1589 178 BstEII G'GTnAC_C Cuts at: 0 846 1767 Size: 846 921 BstXI CCAn_nnnn'nTGG Cuts at: 0 447 1125 1767 Size: 447 678 642 Fragments arranged by size: 678 642 447 BtgI C'CryG_G Cuts at: 0 752 1140 1274 1767 Size: 752 388 134 493 Fragments arranged by size: 752 493 388 134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1665 1767 Size: 1665 102 BtsI GCAGTG_nn' Cuts at: 0 191 1161 1767 Size: 191 970 606 Fragments arranged by size: 970 606 191 DrdI GACnn_nn'nnGTC Cuts at: 0 256 1767 Size: 256 1511 EaeI y'GGCC_r Cuts at: 0 749 879 1127 1137 1190 1424 1767 Size: 749 130 248 10 53 234 343 Fragments arranged by size: 749 343 248 234 130 53 10 EagI C'GGCC_G Cuts at: 0 1190 1767 Size: 1190 577 EarI CTCTTCn'nnn_ Cuts at: 0 136 593 794 895 1767 Size: 136 457 201 101 872 Fragments arranged by size: 872 457 201 136 101 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 541 625 685 1177 1767 Size: 400 141 84 60 492 590 Fragments arranged by size: 590 492 400 141 84 60 EcoNI CCTnn'n_nnAGG Cuts at: 0 1093 1767 Size: 1093 674 EcoO109I rG'GnC_Cy Cuts at: 0 164 1767 Size: 164 1603 EcoRI G'AATT_C Cuts at: 0 1504 1767 Size: 1504 263 HaeII r_GCGC'y Cuts at: 0 675 1767 Size: 675 1092 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 498 530 1087 1119 1767 Size: 498 32 557 32 648 Fragments arranged by size: 648 557 498 32 32 HincII GTy'rAC Cuts at: 0 261 1767 Size: 261 1506 KpnI G_GTAC'C Cuts at: 0 1355 1767 Size: 1355 412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1608 1702 1767 Size: 1608 94 65 Fragments arranged by size: 1608 94 65 MscI TGG'CCA Cuts at: 0 751 881 1129 1139 1767 Size: 751 130 248 10 628 Fragments arranged by size: 751 628 248 130 10 MslI CAynn'nnrTG Cuts at: 0 477 577 1123 1767 Size: 477 100 546 644 Fragments arranged by size: 644 546 477 100 MspA1I CmG'CkG Cuts at: 0 44 561 1462 1767 Size: 44 517 901 305 Fragments arranged by size: 901 517 305 44 NcoI C'CATG_G Cuts at: 0 752 1140 1274 1767 Size: 752 388 134 493 Fragments arranged by size: 752 493 388 134 NsiI A_TGCA'T Cuts at: 0 405 1767 Size: 405 1362 NspI r_CATG'y Cuts at: 0 200 378 407 456 1007 1767 Size: 200 178 29 49 551 760 Fragments arranged by size: 760 551 200 178 49 29 PflMI CCAn_nnn'nTGG Cuts at: 0 573 1599 1767 Size: 573 1026 168 Fragments arranged by size: 1026 573 168 PfoI T'CCnGG_A Cuts at: 0 359 395 416 1767 Size: 359 36 21 1351 Fragments arranged by size: 1351 359 36 21 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 205 237 1767 Size: 205 32 1530 Fragments arranged by size: 1530 205 32 PpuMI rG'GwC_Cy Cuts at: 0 164 1767 Size: 164 1603 PsiI TTA'TAA Cuts at: 0 1526 1767 Size: 1526 241 PstI C_TGCA'G Cuts at: 0 833 1767 Size: 833 934 PvuII CAG'CTG Cuts at: 0 561 1462 1767 Size: 561 901 305 Fragments arranged by size: 901 561 305 SapI GCTCTTCn'nnn_ Cuts at: 0 593 1767 Size: 593 1174 SfcI C'TryA_G Cuts at: 0 829 1767 Size: 829 938 SmaI CCC'GGG Cuts at: 0 325 1767 Size: 325 1442 SmlI C'TyrA_G Cuts at: 0 1330 1767 Size: 1330 437 SphI G_CATG'C Cuts at: 0 200 456 1767 Size: 200 256 1311 Fragments arranged by size: 1311 256 200 StyI C'CwwG_G Cuts at: 0 752 965 1106 1140 1274 1556 1767 Size: 752 213 141 34 134 282 211 Fragments arranged by size: 752 282 213 211 141 134 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 296 1767 Size: 296 1471 TatI w'GTAC_w Cuts at: 0 1025 1767 Size: 1025 742 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 61 93 1767 Size: 61 32 1674 Fragments arranged by size: 1674 61 32 Tth111I GACn'n_nGTC Cuts at: 0 91 1767 Size: 91 1676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 444 600 735 1767 Size: 444 156 135 1032 Fragments arranged by size: 1032 444 156 135 XmaI C'CCGG_G Cuts at: 0 323 1767 Size: 323 1444 XmnI GAAnn'nnTTC Cuts at: 0 959 1767 Size: 959 808 ZraI GAC'GTC Cuts at: 0 141 1767 Size: 141 1626 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI ApaLI ApoI AvaI BaeI BanI BciVI BfrBI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstAPI BstEII BstXI BtgI BtgZI BtsI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI PpiI PpuMI PsiI PstI PvuII SapI SfcI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AgeI AhdI ApaI AscI AseI AsiSI AvrII BamHI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsiWI BspEI BsrBI BsrDI BssHII BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EciI EcoICRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI