################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MboI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 445 GTCTAC GT^[AC][GT]AC AciI 500 GCGG CCGC(-3/-1) AfaI 190 GTAC GT^AC AluI 68 AGCT AG^CT AlwNI 94 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG AspI 439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 9 GGTCC G^G[AT]CC BanII 370 GAGCCC G[GA]GC[CT]^C BciVI 165 GTATCC GTATCC(6/5) BmyI 370 GAGCCC G[AGT]GC[ACT]^C BsaBI 510 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 367 GAGGAG GAGGAG(10/8) BsmFI 96 GGGAC GGGAC(10/14) Bsp1286I 370 GAGCCC G[AGT]GC[ACT]^C BspHI 254 TCATGA T^CATGA BsrI 99 ACTGG ACTGG(1/-1) BstXI 489 CCATGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 131 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 9 GGTCC G^G[ACGT]CC DdeI 273 CTCAG C^T[ACGT]AG MaeIII 457 GTGAC ^GT[ACGT]AC MamI 510 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflFI 439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 12 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 254 TCATGA T^CATGA RsaI 190 GTAC GT^AC SapI 145 GCTCTTC GCTCTTC(1/4) Sau96I 9 GGTCC G^G[ACGT]CC Tsp45I 457 GTGAC ^GT[GC]AC TspRI 462 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 439 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 12 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 519 CCATCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 173 GAATTC [GA]^AATT[CT] 535 GAATTC ApoI 173 GAATTC [GA]^AATT[CT] 535 GAATTC Asp700I 216 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 505 GAACTGATTC BbvI 40 GCAGC GCAGC(8/12) 105 GCTGC BsgI 133 CTGCAC GTGCAG(16/14) 262 GTGCAG BsiYI 12 CCAACCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCAAAAGGAGG BslI 12 CCAACCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCAAAAGGAGG EcoRI 173 GAATTC G^AATTC 535 GAATTC Fnu4HI 40 GCAGC GC^[ACGT]GC 105 GCTGC HincII 337 GTCAAC GT[CT]^[GA]AC 403 GTCAAC HindII 337 GTCAAC GT[CT]^[GA]AC 403 GTCAAC HinfI 53 GATTC G^A[ACGT]TC 510 GATTC HphI 532 GGTGA GGTGA(8/7) 119 TCACC ItaI 40 GCAGC GC^[ACGT]GC 105 GCTGC MslI 255 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 490 CATGTGAATG NlaIII 255 CATG CATG^ 490 CATG NlaIV 8 GGGTCC GG[ACGT]^[ACGT]CC 369 GGAGCC TaqI 56 TCGA T^CGA 171 TCGA TfiI 53 GATTC G^A[AT]TC 510 GATTC TseI 40 GCAGC G^C[AT]GC 105 GCTGC TthHB8I 56 TCGA T^CGA 171 TCGA XmnI 216 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 505 GAACTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BsaJI BssKI BstF5I BstNI EarI EcoRII FokI HaeIII Ksp632I MboII MnlI MvaI ScrFI Tsp509I