(Circular) (Six-base) MAPSORT of: pFN21AB9438.seq Check: 5277 from: 1 to: 5254 pFN21AB9438 5254 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3064 278 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2451 2597 1937 Size: 514 146 4594 Fragments arranged by size: 4594 514 146 Acc65I G'GTAC_C Cuts at: 2575 2575 Size: 5254 AclI AA'CG_TT Cuts at: 1563 3381 3754 1563 Size: 1818 373 3063 Fragments arranged by size: 3063 1818 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2339 2411 3317 4701 4969 1632 Size: 506 201 72 906 1384 268 1917 Fragments arranged by size: 1917 1384 906 506 268 201 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5057 AflIII A'CryG_T Cuts at: 1203 4153 5130 1203 Size: 2950 977 1327 Fragments arranged by size: 2950 1327 977 AgeI A'CCGG_T Cuts at: 4085 4085 Size: 5254 AhdI GACnn_n'nnGTC Cuts at: 3983 3983 Size: 5254 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2163 1184 Size: 979 4275 AlwNI CAG_nnn'CTG Cuts at: 2104 2997 4569 2104 Size: 893 1572 2789 Fragments arranged by size: 2789 1572 893 ApaLI G'TGCA_C Cuts at: 3311 4467 3311 Size: 1156 4098 ApoI r'AATT_y Cuts at: 1493 2178 2540 2563 2789 2941 5050 1493 Size: 685 362 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 685 362 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 3808 160 Size: 872 2776 1606 Fragments arranged by size: 2776 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 AvaI C'yCGr_G Cuts at: 1870 1943 2579 1870 Size: 73 636 4545 Fragments arranged by size: 4545 636 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2153 2186 2153 Size: 33 5221 BamHI G'GATC_C Cuts at: 2584 2951 2584 Size: 367 4887 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2575 4030 618 Size: 325 217 627 788 1455 1842 Fragments arranged by size: 1842 1455 788 627 325 217 BanII G_rGCy'C Cuts at: 1391 2379 2573 1391 Size: 988 194 4072 Fragments arranged by size: 4072 988 194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4283 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3445 3479 1903 Size: 34 1508 34 3678 Fragments arranged by size: 3678 1508 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2181 3146 4356 2181 Size: 965 1210 3079 Fragments arranged by size: 3079 1210 965 BclI T'GATC_A Cuts at: 1548 1548 Size: 5254 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3865 136 Size: 107 122 71 631 605 2193 1525 Fragments arranged by size: 2193 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5249 5249 Size: 5254 BlpI GC'TnA_GC Cuts at: 2671 2671 Size: 5254 Bme1580I G_kGCm'C Cuts at: 1790 3315 4471 1790 Size: 1525 1156 2573 Fragments arranged by size: 2573 1525 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5254 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 2985 3943 Size: 416 53 83 399 24 697 871 958 Cuts at: 3943 442 Size: 1753 Fragments arranged by size: 1753 958 871 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5254 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3914 1239 Size: 2675 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3379 4244 4542 3379 Size: 865 298 4091 Fragments arranged by size: 4091 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3917 882 Size: 617 2418 2219 Fragments arranged by size: 2418 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3045 493 Size: 711 1841 2702 Fragments arranged by size: 2702 1841 711 BsaBI GATnn'nnATC Cuts at: 2519 5248 2519 Size: 2729 2525 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3061 3443 4078 275 Size: 53 83 186 936 1528 382 635 1451 Fragments arranged by size: 1528 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3687 4085 4359 4506 4917 3687 Size: 398 274 147 411 4024 Fragments arranged by size: 4024 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 1178 Size: 30 165 30 1059 30 3940 Fragments arranged by size: 3940 1059 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 1186 Size: 1201 4053 BseYI C'CCAG_C Cuts at: 4457 4953 4457 Size: 496 4758 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2288 1163 Size: 573 387 165 4129 Fragments arranged by size: 4129 573 387 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2704 3465 4493 1540 Size: 275 186 703 761 1028 2301 Fragments arranged by size: 2301 1028 761 703 275 186 BsiHKAI G_wGCw'C Cuts at: 3315 3400 4471 3315 Size: 85 1071 4098 Fragments arranged by size: 4098 1071 85 BsmI GAATG_Cn' Cuts at: 2765 2858 2765 Size: 93 5161 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2379 2573 3315 3400 4471 1391 Size: 399 589 194 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 589 399 194 85 BspEI T'CCGG_A Cuts at: 4917 4917 Size: 5254 BspHI T'CATG_A Cuts at: 2259 3143 4873 2259 Size: 884 1730 2640 Fragments arranged by size: 2640 1730 884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2609 844 Size: 1765 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3141 1394 Size: 429 1318 3507 Fragments arranged by size: 3507 1318 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3749 3923 65 Size: 1183 2501 174 1396 Fragments arranged by size: 2501 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3898 4085 1700 Size: 110 2088 187 2869 Fragments arranged by size: 2869 2088 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5254 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5254 BssSI C'ACGA_G Cuts at: 3314 4326 3314 Size: 1012 4242 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5254 BstBI TT'CG_AA Cuts at: 4058 4058 Size: 5254 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5254 BstXI CCAn_nnnn'nTGG Cuts at: 2501 2501 Size: 5254 BstYI r'GATC_y Cuts at: 1972 2584 2951 3336 3353 4805 4891 4903 5249 Size: 612 367 385 17 1452 86 12 346 Cuts at: 5249 1972 Size: 1977 Fragments arranged by size: 1977 1452 612 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3763 Fragments arranged by size: 3763 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4515 Fragments arranged by size: 4515 683 56 BtsI GCAGTG_nn' Cuts at: 2770 3564 3584 2770 Size: 794 20 4440 Fragments arranged by size: 4440 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5254 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5219 DraI TTT'AAA Cuts at: 2558 2558 Size: 5254 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4261 809 Size: 507 2945 1802 Fragments arranged by size: 2945 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2701 3590 5219 Size: 54 1425 50 275 18 871 889 1629 Cuts at: 5219 8 Size: 43 Fragments arranged by size: 1629 1425 889 871 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2701 1537 Size: 275 889 4090 Fragments arranged by size: 4090 889 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2157 2387 3184 1353 Size: 669 135 230 797 3423 Fragments arranged by size: 3423 797 669 230 135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2520 3841 4215 4361 387 Size: 1064 138 931 1321 374 146 1280 Fragments arranged by size: 1321 1280 1064 931 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2339 2411 3317 3914 4701 4969 Size: 393 506 201 72 906 597 787 268 Cuts at: 4969 1239 Size: 1524 Fragments arranged by size: 1524 906 787 597 506 393 268 201 72 EcoRI G'AATT_C Cuts at: 2178 2540 2563 2941 2178 Size: 362 23 378 4491 Fragments arranged by size: 4491 378 362 23 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2344 2344 Size: 5254 FspI TGC'GCA Cuts at: 3760 3760 Size: 5254 HaeII r_GCGC'y Cuts at: 1678 4401 1678 Size: 2723 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 3895 3927 3969 Size: 32 135 32 890 32 1401 32 42 Cuts at: 3969 4001 1373 Size: 32 2626 Fragments arranged by size: 2626 1401 890 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 2410 2598 2844 669 Size: 988 281 406 66 188 246 3079 Fragments arranged by size: 3079 988 406 281 246 188 66 HindIII A'AGCT_T Cuts at: 748 748 Size: 5254 HpaI GTT'AAC Cuts at: 2844 2844 Size: 5254 KpnI G_GTAC'C Cuts at: 2579 2579 Size: 5254 MfeI C'AATT_G Cuts at: 2853 2853 Size: 5254 MluI A'CGCG_T Cuts at: 5130 5130 Size: 5254 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2041 2410 4368 4552 1305 Size: 736 369 1958 184 2007 Fragments arranged by size: 2007 1958 736 369 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3432 Fragments arranged by size: 3432 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2499 3212 3571 3730 518 Size: 648 1098 235 713 359 159 2042 Fragments arranged by size: 2042 1098 713 648 359 235 159 MspA1I CmG'CkG Cuts at: 1606 2670 3347 4495 4740 1606 Size: 1064 677 1148 245 2120 Fragments arranged by size: 2120 1148 1064 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3763 Fragments arranged by size: 3763 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5254 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5254 NotI GC'GGCC_GC Cuts at: 2701 2701 Size: 5254 NspI r_CATG'y Cuts at: 1132 1229 2612 4157 1132 Size: 97 1383 1545 2229 Fragments arranged by size: 2229 1545 1383 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5254 PciI A'CATG_T Cuts at: 4153 4153 Size: 5254 PflMI CCAn_nnn'nTGG Cuts at: 1108 2023 1108 Size: 915 4339 PmeI GTTT'AAAC Cuts at: 2558 2558 Size: 5254 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3323 3355 4836 4868 3323 Size: 32 1481 32 3709 Fragments arranged by size: 3709 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5254 PsiI TTA'TAA Cuts at: 2824 2824 Size: 5254 PstI C_TGCA'G Cuts at: 830 2606 830 Size: 1776 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5254 SalI G'TCGA_C Cuts at: 1936 2596 1936 Size: 660 4594 SapI GCTCTTCn'nnn_ Cuts at: 2157 2157 Size: 5254 SbfI CC_TGCA'GG Cuts at: 2606 2606 Size: 5254 ScaI AGT'ACT Cuts at: 3502 5214 3502 Size: 1712 3542 SfcI C'TryA_G Cuts at: 826 1045 2602 3737 4418 4609 826 Size: 219 1557 1135 681 191 1471 Fragments arranged by size: 1557 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5254 SmaI CCC'GGG Cuts at: 1872 2581 1872 Size: 709 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3358 4259 4521 820 Size: 197 926 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3045 493 Size: 2552 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5254 SphI G_CATG'C Cuts at: 1132 2612 1132 Size: 1480 3774 SspI AAT'ATT Cuts at: 5 52 3178 5 Size: 47 3126 2081 Fragments arranged by size: 3126 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5254 StyI C'CwwG_G Cuts at: 513 1065 2004 2693 513 Size: 552 939 689 3074 Fragments arranged by size: 3074 939 689 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3281 3298 3451 3636 4081 325 Size: 1255 1701 17 153 185 445 1498 Fragments arranged by size: 1701 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2194 3500 5212 Size: 274 80 33 51 1443 217 1306 1712 Cuts at: 5212 96 Size: 138 Fragments arranged by size: 1712 1443 1306 274 217 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5214 5246 5214 Size: 32 5222 Tth111I GACn'n_nGTC Cuts at: 1316 2447 1316 Size: 1131 4123 XbaI T'CTAG_A Cuts at: 2590 2590 Size: 5254 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2531 2531 Size: 5254 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5254 XmaI C'CCGG_G Cuts at: 1870 2579 1870 Size: 709 4545 XmnI GAAnn'nnTTC Cuts at: 2225 2514 3383 2225 Size: 289 869 4096 Fragments arranged by size: 4096 869 289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3062 276 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI