Restriction map for
pFN21AE1079
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspEI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraII
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoO109I EcoO65I FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 2390 GTCTAC GT^[AC][GT]AC
AlwNI 2618 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 133 AGCGCT AGC^GCT
Asp700I 2947 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1340 GTGCTC G[AT]GC[AT]^C
AspI 2046 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1860 GGATCC G^GATCC
BanI 3031 GGTGCC G^G[CT][GA]CC
BciVI 3135 GGATAC GTATCC(6/5)
BglII 2713 AGATCT A^GATCT
BmyI 1340 GTGCTC G[AGT]GC[ACT]^C
BpmI 1619 CTCCAG CTGGAG(16/14)
BsgI 1580 CTGCAC GTGCAG(16/14)
BsiHKAI 1340 GTGCTC G[AT]GC[AT]^C
Bsp1286I 1340 GTGCTC G[AGT]GC[ACT]^C
BspDI 2113 ATCGAT AT^CGAT
BspHI 2292 TCATGA T^CATGA
BspMI 1086 GCAGGT ACCTGC(4/8)
BstAPI 1004 GCATTCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 134 GCGC GCG^C
ClaI 2113 ATCGAT AT^CGAT
DraIII 400 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2550 GAAGAG CTCTTC(1/4)
Eco47III 133 AGCGCT AGC^GCT
EcoRI 2296 GAATTC G^AATTC
EcoRV 2137 GATATC GAT^ATC
EcoT14I 2402 CCAAGG C^C[AT][AT]GG
FauI 180 GCGGG CCCGC(4/6)
HaeII 133 AGCGCT [GA]GCGC^[CT]
HapII 1734 CCGG C^CGG
HhaI 134 GCGC GCG^C
HinP1I 134 GCGC G^CGC
HindIII 174 AAGCTT A^AGCTT
HpaII 1734 CCGG C^CGG
Ksp632I 2550 GAAGAG CTCTTC(1/4)
MspI 1734 CCGG C^CGG
NdeI 2923 CATATG CA^TATG
PflFI 2046 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2292 TCATGA T^CATGA
SexAI 27 ACCTGGT A^CC[AT]GGT
SfcI 657 CTATAG C^T[GA][CT]AG
SphI 297 GCATGC GCATG^C
SspI 1231 AATATT AAT^ATT
StuI 1998 AGGCCT AGG^CCT
StyI 2402 CCAAGG C^C[AT][AT]GG
Tth111I 2046 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2572 CCATCTTCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2947 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 1279 GGACC G^G[AT]CC
2406 GGACC
BalI 681 TGGCCA TGG^CCA
1879 TGGCCA
BseRI 2627 CTCCTC GAGGAG(10/8)
3000 CTCCTC
BsiYI 82 CCTACCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2001 CCTTGTAGAGG
BslI 82 CCTACCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2001 CCTTGTAGAGG
BsmAI 3088 GAGAC GTCTC(1/5)
994 GTCTC
Bsp1407I 138 TGTACA T^GTACA
2352 TGTACA
BsrGI 138 TGTACA T^GTACA
2352 TGTACA
DrdI 194 GACATGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1805 GACAGCATAGTC
EaeI 681 TGGCCA [CT]^GGCC[GA]
1879 TGGCCA
EcoNI 82 CCTACCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2001 CCTTGTAGAGG
EcoT22I 1002 ATGCAT ATGCA^T
3041 ATGCAT
MaeII 2205 ACGT A^CGT
2528 ACGT
MfeI 154 CAATTG C^AATTG
734 CAATTG
MluNI 681 TGGCCA TGG^CCA
1879 TGGCCA
MscI 681 TGGCCA TGG^CCA
1879 TGGCCA
MunI 154 CAATTG C^AATTG
734 CAATTG
NsiI 1002 ATGCAT ATGCA^T
3041 ATGCAT
SspBI 138 TGTACA T^GTACA
2352 TGTACA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BbsI BbvI BclI
BfaI BpuAI BsaJI BsmFI BsmI
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EcoRII FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII