Restriction map for
pFN21AE1079
(Circular) (Six-base) MAPSORT of: pFN21AE1079.seq Check: 3743 from: 1 to: 7921
pFN21AE1079 7921 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5731 278
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4396 5264 1937
Size: 2459 868 4594
Fragments arranged by size:
4594 2459 868
Acc65I G'GTAC_C
Cuts at: 5242 5242
Size: 7921
AclI AA'CG_TT
Cuts at: 1563 6048 6421 1563
Size: 4485 373 3063
Fragments arranged by size:
4485 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4156 4214 4884 5984 7368 7636 1632
Size: 2524 58 670 1100 1384 268 1917
Fragments arranged by size:
2524 1917 1384 1100 670 268 58
AfeI AGC'GCT
Cuts at: 2140 2140
Size: 7921
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7724
AflIII A'CryG_T
Cuts at: 1203 2200 2430 4354 6820 7797 1203
Size: 997 230 1924 2466 977 1327
Fragments arranged by size:
2466 1924 1327 997 977 230
AgeI A'CCGG_T
Cuts at: 6752 6752
Size: 7921
AhdI GACnn_n'nnGTC
Cuts at: 6650 6650
Size: 7921
AleI CACnn'nnGTG
Cuts at: 1166 3270 1166
Size: 2104 5817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2325 1184
Size: 1141 6780
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4670 4702 4670
Size: 32 7889
AlwNI CAG_nnn'CTG
Cuts at: 4628 5664 7236 4628
Size: 1036 1572 5313
Fragments arranged by size:
5313 1572 1036
ApaLI G'TGCA_C
Cuts at: 5978 7134 5978
Size: 1156 6765
ApoI r'AATT_y
Cuts at: 1493 2018 2540 2700 3221 4273 4301 4478 4504
Size: 525 522 160 521 1052 28 177 26
Cuts at: 4504 5230 5456 5608 7717 1493
Size: 726 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 726 525 522 521 226
177 160 152 28 26
AseI AT'TA_AT
Cuts at: 160 1032 6475 160
Size: 872 5443 1606
Fragments arranged by size:
5443 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
AvaI C'yCGr_G
Cuts at: 1870 1943 5246 1870
Size: 73 3303 4545
Fragments arranged by size:
4545 3303 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2419 2452 2419
Size: 33 7888
BamHI G'GATC_C
Cuts at: 3865 5251 5618 3865
Size: 1386 367 6168
Fragments arranged by size:
6168 1386 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5036 5242 6697 618
Size: 325 217 627 3249 206 1455 1842
Fragments arranged by size:
3249 1842 1455 627 325 217 206
BanII G_rGCy'C
Cuts at: 1391 5240 1391
Size: 3849 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 3456 4371 4584 928
Size: 971 347 1210 915 213 4265
Fragments arranged by size:
4265 1210 971 915 347 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6112 6146 1903
Size: 34 4175 34 3678
Fragments arranged by size:
4175 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5134 5813 7023 5134
Size: 679 1210 6032
Fragments arranged by size:
6032 1210 679
BclI T'GATC_A
Cuts at: 1548 3193 3307 4075 1548
Size: 1645 114 768 5394
Fragments arranged by size:
5394 1645 768 114
BfrBI ATG'CAT
Cuts at: 3009 5048 3009
Size: 2039 5882
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6532 136
Size: 107 122 71 631 605 4860 1525
Fragments arranged by size:
4860 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4718 7916 4718
Size: 3198 4723
BlpI GC'TnA_GC
Cuts at: 5338 5338
Size: 7921
Bme1580I G_kGCm'C
Cuts at: 1790 5982 7138 1790
Size: 4192 1156 2573
Fragments arranged by size:
4192 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5652 6610 442
Size: 416 53 83 399 24 4235 958 1753
Fragments arranged by size:
4235 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3609 6581 1239
Size: 2370 2972 2579
Fragments arranged by size:
2972 2579 2370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6046 6911 7209 6046
Size: 865 298 6758
Fragments arranged by size:
6758 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6584 882
Size: 617 5085 2219
Fragments arranged by size:
5085 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5712 493
Size: 711 4508 2702
Fragments arranged by size:
4508 2702 711
BsaBI GATnn'nnATC
Cuts at: 7915 7915
Size: 7921
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5728 6110 6745 275
Size: 53 83 186 936 4195 382 635 1451
Fragments arranged by size:
4195 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6354 6752 7026 7173 7584 6354
Size: 398 274 147 411 6691
Fragments arranged by size:
6691 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3247 3277 4208 4238 1178
Size: 30 165 30 1844 30 931 30 4861
Fragments arranged by size:
4861 1844 931 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4623 4996 1186
Size: 3437 373 4111
Fragments arranged by size:
4111 3437 373
BseYI C'CCAG_C
Cuts at: 7124 7620 7124
Size: 496 7425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3570 1163
Size: 573 1834 5514
Fragments arranged by size:
5514 1834 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5371 6132 7160 1540
Size: 275 186 3370 761 1028 2301
Fragments arranged by size:
3370 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3349 5982 6067 7138 3349
Size: 2633 85 1071 4132
Fragments arranged by size:
4132 2633 1071 85
BsmI GAATG_Cn'
Cuts at: 2764 3009 4478 5432 5525 2764
Size: 245 1469 954 93 5160
Fragments arranged by size:
5160 1469 954 245 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3349 5240 5982 6067 7138 1391
Size: 399 1559 1891 742 85 1071 2174
Fragments arranged by size:
2174 1891 1559 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7584 7584
Size: 7921
BspHI T'CATG_A
Cuts at: 4297 5810 7540 4297
Size: 1513 1730 4678
Fragments arranged by size:
4678 1730 1513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3082 5276 844
Size: 2238 2194 3489
Fragments arranged by size:
3489 2238 2194
BsrBI CCG'CTC
Cuts at: 1394 1823 5808 1394
Size: 429 3985 3507
Fragments arranged by size:
3985 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6416 6590 65
Size: 1183 5168 174 1396
Fragments arranged by size:
5168 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6565 6752 1700
Size: 110 4755 187 2869
Fragments arranged by size:
4755 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2143 4357 96
Size: 2047 2214 3660
Fragments arranged by size:
3660 2214 2047
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7921
BssSI C'ACGA_G
Cuts at: 5981 6993 5981
Size: 1012 6909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3015 794
Size: 2221 5700
BstBI TT'CG_AA
Cuts at: 6725 6725
Size: 7921
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7921
BstYI r'GATC_y
Cuts at: 1972 2521 3865 4718 5251 5618 6003 6020 7472
Size: 549 1344 853 533 367 385 17 1452
Cuts at: 7472 7558 7570 7916 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1344 853 549 533 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6430
Fragments arranged by size:
6430 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7182
Fragments arranged by size:
7182 683 56
BtsI GCAGTG_nn'
Cuts at: 4078 5200 5437 6231 6251 4078
Size: 1122 237 794 20 5748
Fragments arranged by size:
5748 1122 794 237 20
ClaI AT'CG_AT
Cuts at: 1554 4119 1554
Size: 2565 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3018 3053 4752 4787 529
Size: 35 2454 35 1699 35 3663
Fragments arranged by size:
3663 2454 1699 35 35 35
DraI TTT'AAA
Cuts at: 2611 3174 3499 4858 5225 2611
Size: 563 325 1359 367 5307
Fragments arranged by size:
5307 1359 563 367 325
DraIII CAC_nnn'GTG
Cuts at: 2410 2410
Size: 7921
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2205 3816 6928 809
Size: 507 889 1611 3112 1802
Fragments arranged by size:
3112 1802 1611 889 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2686 3884 5368
Size: 54 1425 50 275 18 856 1198 1484
Cuts at: 5368 6257 7886 8
Size: 889 1629 43
Fragments arranged by size:
1629 1484 1425 1198 889 856 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5368 1537
Size: 275 3556 4090
Fragments arranged by size:
4090 3556 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4550 5851 1353
Size: 3197 1301 3423
Fragments arranged by size:
3423 3197 1301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6508 6882 7028 387
Size: 1064 138 4919 374 146 1280
Fragments arranged by size:
4919 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3609 4156 4214 4884 5984 6581 7368
Size: 393 1977 547 58 670 1100 597 787
Cuts at: 7368 7636 1239
Size: 268 1524
Fragments arranged by size:
1977 1524 1100 787 670 597 547 393
268 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2091 4010 2091
Size: 1919 6002
EcoRI G'AATT_C
Cuts at: 4301 5230 5608 4301
Size: 929 378 6614
Fragments arranged by size:
6614 929 378
EcoRV GAT'ATC
Cuts at: 4144 4144
Size: 7921
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4914 4914
Size: 7921
FspI TGC'GCA
Cuts at: 6427 6427
Size: 7921
HaeII r_GCGC'y
Cuts at: 1678 2142 7068 1678
Size: 464 4926 2531
Fragments arranged by size:
4926 2531 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2984 3016 3199 3231 3247
Size: 32 135 32 1412 32 183 32 16
Cuts at: 3247 3279 3607 3639 4208 4225 4240 4257 4416
Size: 32 328 32 569 17 15 17 159
Cuts at: 4416 4448 6562 6594 6636 6668 1373
Size: 32 2114 32 42 32 2626
Fragments arranged by size:
2626 2114 1412 569 328 183 159 135
42 32 32 32 32 32 32 32
32 32 17 17 16 15
HincII GTy'rAC
Cuts at: 669 1657 1938 5265 5511 669
Size: 988 281 3327 246 3079
Fragments arranged by size:
3327 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2179 748
Size: 1431 6490
HpaI GTT'AAC
Cuts at: 5511 5511
Size: 7921
KpnI G_GTAC'C
Cuts at: 5246 5246
Size: 7921
MfeI C'AATT_G
Cuts at: 2159 2739 5520 2159
Size: 580 2781 4560
Fragments arranged by size:
4560 2781 580
MluI A'CGCG_T
Cuts at: 7797 7797
Size: 7921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2197 2284 2367 2571 3251 3731 7035 7219
Size: 892 87 83 204 680 480 3304 184
Cuts at: 7219 1305
Size: 2007
Fragments arranged by size:
3304 2007 892 680 480 204 184 87
83
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2688 3886 10
Size: 54 1425 343 856 1198 4045
Fragments arranged by size:
4045 1425 1198 856 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2907 3270 4359 4932 5879 6238 6397
Size: 648 1741 363 1089 573 947 359 159
Cuts at: 6397 518
Size: 2042
Fragments arranged by size:
2042 1741 1089 947 648 573 363 359
159
MspA1I CmG'CkG
Cuts at: 1606 2124 3102 3717 5337 6014 7162 7407 1606
Size: 518 978 615 1620 677 1148 245 2120
Fragments arranged by size:
2120 1620 1148 978 677 615 518 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6430
Fragments arranged by size:
6430 939 552
NdeI CA'TA_TG
Cuts at: 387 4929 387
Size: 4542 3379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7921
NotI GC'GGCC_GC
Cuts at: 5368 5368
Size: 7921
NsiI A_TGCA'T
Cuts at: 3011 5050 3011
Size: 2039 5882
NspI r_CATG'y
Cuts at: 1132 1229 2204 2306 2434 4358 5279 6824 1132
Size: 97 975 102 128 1924 921 1545 2229
Fragments arranged by size:
2229 1924 1545 975 921 128 102 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7921
PciI A'CATG_T
Cuts at: 2200 2430 4354 6820 2200
Size: 230 1924 2466 3301
Fragments arranged by size:
3301 2466 1924 230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 7019
PfoI T'CCnGG_A
Cuts at: 3625 3625
Size: 7921
PmeI GTTT'AAAC
Cuts at: 5225 5225
Size: 7921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5990 6022 7503 7535 5990
Size: 32 1481 32 6376
Fragments arranged by size:
6376 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7921
PsiI TTA'TAA
Cuts at: 5491 5491
Size: 7921
PstI C_TGCA'G
Cuts at: 830 5273 830
Size: 4443 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7921
PvuII CAG'CTG
Cuts at: 2124 3102 3717 2124
Size: 978 615 6328
Fragments arranged by size:
6328 978 615
SalI G'TCGA_C
Cuts at: 1936 5263 1936
Size: 3327 4594
SbfI CC_TGCA'GG
Cuts at: 5273 5273
Size: 7921
ScaI AGT'ACT
Cuts at: 6169 7881 6169
Size: 1712 6209
SexAI A'CCwGG_T
Cuts at: 2032 2032
Size: 7921
SfcI C'TryA_G
Cuts at: 826 1045 2662 5269 6404 7085 7276 826
Size: 219 1617 2607 1135 681 191 1471
Fragments arranged by size:
2607 1617 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7921
SmaI CCC'GGG
Cuts at: 1872 5248 1872
Size: 3376 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6025 6926 7188 820
Size: 197 926 4082 901 262 1553
Fragments arranged by size:
4082 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5712 493
Size: 5219 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7921
SphI G_CATG'C
Cuts at: 1132 2306 5279 1132
Size: 1174 2973 3774
Fragments arranged by size:
3774 2973 1174
SspI AAT'ATT
Cuts at: 5 52 3238 5845 5
Size: 47 3186 2607 2081
Fragments arranged by size:
3186 2607 2081 47
StuI AGG'CCT
Cuts at: 1517 4005 1517
Size: 2488 5433
StyI C'CwwG_G
Cuts at: 513 1065 2004 4407 5360 513
Size: 552 939 2403 953 3074
Fragments arranged by size:
3074 2403 953 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2792 3573 4323 4695 4716 5948 5965
Size: 1255 1212 781 750 372 21 1232 17
Cuts at: 5965 6118 6303 6748 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1232 1212 781 750 445 372
185 153 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2143 4357 4892
Size: 274 80 33 51 1443 166 2214 535
Cuts at: 4892 4922 6167 7879 96
Size: 30 1245 1712 138
Fragments arranged by size:
2214 1712 1443 1245 535 274 166 138
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 7881 7913 2020
Size: 32 5829 32 2028
Fragments arranged by size:
5829 2028 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4054 1316
Size: 2738 5183
XbaI T'CTAG_A
Cuts at: 5257 5257
Size: 7921
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4584 4584
Size: 7921
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7921
XmaI C'CCGG_G
Cuts at: 1870 5246 1870
Size: 3376 4545
XmnI GAAnn'nnTTC
Cuts at: 4956 6050 4956
Size: 1094 6827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5729 276
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI