(Circular) (Six-base) MAPSORT of: pFN21AB5616.seq Check: 6926 from: 1 to: 7069 pFN21AB5616 7069 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2389 2639 3209 3788 3899 2389 Size: 250 570 579 111 5559 Fragments arranged by size: 5559 579 570 250 111 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4879 278 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4337 4412 1937 Size: 2400 75 4594 Fragments arranged by size: 4594 2400 75 Acc65I G'GTAC_C Cuts at: 2486 4390 2486 Size: 1904 5165 AclI AA'CG_TT Cuts at: 1563 5196 5569 1563 Size: 3633 373 3063 Fragments arranged by size: 3633 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 2237 3192 3256 5132 6516 6784 1632 Size: 590 15 955 64 1876 1384 268 1917 Fragments arranged by size: 1917 1876 1384 955 590 268 64 15 AflII C'TTAA_G Cuts at: 820 1017 4016 820 Size: 197 2999 3873 Fragments arranged by size: 3873 2999 197 AflIII A'CryG_T Cuts at: 1203 2574 2656 3767 5968 6945 1203 Size: 1371 82 1111 2201 977 1327 Fragments arranged by size: 2201 1371 1327 1111 977 82 AgeI A'CCGG_T Cuts at: 5900 5900 Size: 7069 AhdI GACnn_n'nnGTC Cuts at: 5798 5798 Size: 7069 AleI CACnn'nnGTG Cuts at: 1166 3612 1166 Size: 2446 4623 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3815 1184 Size: 2631 4438 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3121 3153 3121 Size: 32 7037 AlwNI CAG_nnn'CTG Cuts at: 2131 2482 3014 4812 6384 2131 Size: 351 532 1798 1572 2816 Fragments arranged by size: 2816 1798 1572 532 351 ApaI G_GGCC'C Cuts at: 2088 4154 2088 Size: 2066 5003 ApaLI G'TGCA_C Cuts at: 5126 6282 5126 Size: 1156 5913 ApoI r'AATT_y Cuts at: 1493 2377 4259 4268 4378 4604 4756 6865 1493 Size: 884 1882 9 110 226 152 2109 1697 Fragments arranged by size: 2109 1882 1697 884 226 152 110 9 AseI AT'TA_AT Cuts at: 160 1032 5623 160 Size: 872 4591 1606 Fragments arranged by size: 4591 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 AvaI C'yCGr_G Cuts at: 1870 1943 2265 3546 4394 1870 Size: 73 322 1281 848 4545 Fragments arranged by size: 4545 1281 848 322 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2080 2113 2470 2503 2080 Size: 33 357 33 6646 Fragments arranged by size: 6646 357 33 33 BamHI G'GATC_C Cuts at: 3485 4399 4766 3485 Size: 914 367 5788 Fragments arranged by size: 5788 914 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2486 4390 5845 618 Size: 325 217 627 699 1904 1455 1842 Fragments arranged by size: 1904 1842 1455 699 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2117 2144 2186 2303 3054 3860 3955 Size: 697 29 27 42 117 751 806 95 Cuts at: 3955 4154 4388 1391 Size: 199 234 4072 Fragments arranged by size: 4072 806 751 697 234 199 117 95 42 29 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2630 928 Size: 971 731 5367 Fragments arranged by size: 5367 971 731 BbvCI CC'TCA_GC Cuts at: 2288 2288 Size: 7069 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5260 5294 1903 Size: 34 50 34 3239 34 3678 Fragments arranged by size: 3678 3239 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 4961 6171 3857 Size: 1104 1210 4755 Fragments arranged by size: 4755 1210 1104 BclI T'GATC_A Cuts at: 1548 1548 Size: 7069 BfrBI ATG'CAT Cuts at: 2807 2807 Size: 7069 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3395 3478 3576 Size: 107 122 71 631 605 1723 83 98 Cuts at: 3576 5680 136 Size: 2104 1525 Fragments arranged by size: 2104 1723 1525 631 605 122 107 98 83 71 BglII A'GATC_T Cuts at: 7064 7064 Size: 7069 BlpI GC'TnA_GC Cuts at: 4486 4486 Size: 7069 Bme1580I G_kGCm'C Cuts at: 1790 2088 2233 3887 4154 4367 5130 6286 1790 Size: 298 145 1654 267 213 763 1156 2573 Fragments arranged by size: 2573 1654 1156 763 298 267 213 145 BmgBI CAC'GTC Cuts at: 1318 4335 1318 Size: 3017 4052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2246 3484 4800 Size: 416 53 83 399 24 829 1238 1316 Cuts at: 4800 5758 442 Size: 958 1753 Fragments arranged by size: 1753 1316 1238 958 829 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7069 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3706 3706 Size: 7069 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2815 3089 5729 1239 Size: 1086 490 274 2640 2579 Fragments arranged by size: 2640 2579 1086 490 274 Bpu10I CC'TnA_GC Cuts at: 2288 3691 2288 Size: 1403 5666 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2310 3215 3233 5194 6059 6357 2310 Size: 905 18 1961 865 298 3022 Fragments arranged by size: 3022 1961 905 865 298 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5732 882 Size: 617 4233 2219 Fragments arranged by size: 4233 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2659 4356 4860 493 Size: 711 1455 1697 504 2702 Fragments arranged by size: 2702 1697 1455 711 504 BsaBI GATnn'nnATC Cuts at: 7063 7063 Size: 7069 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4876 5258 5893 275 Size: 53 83 186 936 3343 382 635 1451 Fragments arranged by size: 3343 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5502 5900 6174 6321 6732 5502 Size: 398 274 147 411 5839 Fragments arranged by size: 5839 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2340 2370 2915 2945 3249 Size: 30 165 30 937 30 545 30 304 Cuts at: 3249 3279 3644 3674 1178 Size: 30 365 30 4573 Fragments arranged by size: 4573 937 545 365 304 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3521 4223 1186 Size: 2335 702 4032 Fragments arranged by size: 4032 2335 702 BseYI C'CCAG_C Cuts at: 2053 3481 3525 6272 6768 2053 Size: 1428 44 2747 496 2354 Fragments arranged by size: 2747 2354 1428 496 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3727 3826 3889 1163 Size: 573 1991 99 63 4343 Fragments arranged by size: 4343 1991 573 99 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 2030 3814 4252 4519 5280 Size: 275 186 17 12 1784 438 267 761 Cuts at: 5280 6308 1540 Size: 1028 2301 Fragments arranged by size: 2301 1784 1028 761 438 275 267 186 17 12 BsiHKAI G_wGCw'C Cuts at: 2117 2162 3054 3503 3692 5130 5215 6286 2117 Size: 45 892 449 189 1438 85 1071 2900 Fragments arranged by size: 2900 1438 1071 892 449 189 85 45 BsmI GAATG_Cn' Cuts at: 3542 4580 4673 3542 Size: 1038 93 5938 Fragments arranged by size: 5938 1038 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2117 2144 2162 2186 2233 2303 Size: 399 298 29 27 18 24 47 70 Cuts at: 2303 3054 3503 3692 3860 3887 3955 4154 4367 Size: 751 449 189 168 27 68 199 213 Cuts at: 4367 4388 5130 5215 6286 1391 Size: 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 751 742 449 399 298 213 199 189 168 85 70 68 47 29 27 27 24 21 18 BspEI T'CCGG_A Cuts at: 6732 6732 Size: 7069 BspHI T'CATG_A Cuts at: 4958 6688 4958 Size: 1730 5339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2389 2535 2639 3209 3788 3899 3980 4424 Size: 1545 146 104 570 579 111 81 444 Cuts at: 4424 844 Size: 3489 Fragments arranged by size: 3489 1545 579 570 444 146 111 104 81 BsrBI CCG'CTC Cuts at: 1394 1823 2032 4956 1394 Size: 429 209 2924 3507 Fragments arranged by size: 3507 2924 429 209 BsrDI GCAATG_nn' Cuts at: 65 1248 3103 3442 3799 5564 5738 65 Size: 1183 1855 339 357 1765 174 1396 Fragments arranged by size: 1855 1765 1396 1183 357 339 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3743 5713 5900 1700 Size: 110 1933 1970 187 2869 Fragments arranged by size: 2869 1970 1933 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7069 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7069 BssSI C'ACGA_G Cuts at: 5129 6141 5129 Size: 1012 6057 BstAPI GCAn_nnn'nTGC Cuts at: 794 3642 794 Size: 2848 4221 BstBI TT'CG_AA Cuts at: 2372 5873 2372 Size: 3501 3568 BstEII G'GTnAC_C Cuts at: 1184 2680 3367 1184 Size: 1496 687 4886 Fragments arranged by size: 4886 1496 687 BstXI CCAn_nnnn'nTGG Cuts at: 2941 2941 Size: 7069 BstYI r'GATC_y Cuts at: 1972 3485 4399 4766 5151 5168 6620 6706 6718 Size: 1513 914 367 385 17 1452 86 12 Cuts at: 6718 7064 1972 Size: 346 1977 Fragments arranged by size: 1977 1513 1452 914 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2018 2940 3084 3336 3697 3814 513 Size: 552 953 922 144 252 361 117 3768 Fragments arranged by size: 3768 953 922 552 361 252 144 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3404 487 Size: 683 56 2178 4152 Fragments arranged by size: 4152 2178 683 56 BtsI GCAGTG_nn' Cuts at: 2132 2903 3015 3619 4044 4585 5379 5399 2132 Size: 771 112 604 425 541 794 20 3802 Fragments arranged by size: 3802 794 771 604 541 425 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7034 DraI TTT'AAA Cuts at: 4373 4373 Size: 7069 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4073 6076 809 Size: 507 2757 2003 1802 Fragments arranged by size: 2757 2003 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2027 2360 Size: 54 1425 50 275 18 185 12 333 Cuts at: 2360 2496 3700 3811 4516 5405 7034 8 Size: 136 1204 111 705 889 1629 43 Fragments arranged by size: 1629 1425 1204 889 705 333 275 185 136 111 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2015 2027 3811 4516 1537 Size: 275 203 12 1784 705 4090 Fragments arranged by size: 4090 1784 705 275 203 12 EarI CTCTTCn'nnn_ Cuts at: 1353 3168 4125 4999 1353 Size: 1815 957 874 3423 Fragments arranged by size: 3423 1815 957 874 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5656 6030 6176 387 Size: 1064 138 4067 374 146 1280 Fragments arranged by size: 4067 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2115 3052 2115 Size: 937 6132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2237 2325 2815 3089 3192 3256 Size: 393 590 15 88 490 274 103 64 Cuts at: 3256 5132 5729 6516 6784 1239 Size: 1876 597 787 268 1524 Fragments arranged by size: 1876 1524 787 597 590 490 393 274 268 103 88 64 15 EcoO109I rG'GnC_Cy Cuts at: 2084 2170 2283 2596 3441 4150 4151 2084 Size: 86 113 313 845 709 1 5002 Fragments arranged by size: 5002 845 709 313 113 86 1 EcoRI G'AATT_C Cuts at: 4259 4378 4756 4259 Size: 119 378 6572 Fragments arranged by size: 6572 378 119 FspI TGC'GCA Cuts at: 5575 5575 Size: 7069 HaeII r_GCGC'y Cuts at: 1678 2054 2063 6216 1678 Size: 376 9 4153 2531 Fragments arranged by size: 4153 2531 376 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2936 2963 2968 Size: 32 135 32 713 32 619 27 5 Cuts at: 2968 2995 3644 3676 3986 4018 4319 4351 5710 Size: 27 649 32 310 32 301 32 1359 Cuts at: 5710 5742 5784 5816 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1359 713 649 619 310 301 135 42 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 4413 4659 669 Size: 988 281 2475 246 3079 Fragments arranged by size: 3079 2475 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7069 HpaI GTT'AAC Cuts at: 4659 4659 Size: 7069 KpnI G_GTAC'C Cuts at: 2490 4394 2490 Size: 1904 5165 MfeI C'AATT_G Cuts at: 4668 4668 Size: 7069 MluI A'CGCG_T Cuts at: 3767 6945 3767 Size: 3178 3891 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3759 6183 6367 1305 Size: 2454 2424 184 2007 Fragments arranged by size: 2454 2424 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 3702 10 Size: 54 1425 343 666 1204 3377 Fragments arranged by size: 3377 1425 1204 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 2504 2939 3612 3782 4163 5027 Size: 648 1224 114 435 673 170 381 864 Cuts at: 5027 5386 5545 518 Size: 359 159 2042 Fragments arranged by size: 2042 1224 864 673 648 435 381 359 170 159 114 MspA1I CmG'CkG Cuts at: 1606 2020 3574 3711 3810 3816 3837 4485 5162 Size: 414 1554 137 99 6 21 648 677 Cuts at: 5162 6310 6555 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1554 1148 677 648 414 245 137 99 21 6 NcoI C'CATG_G Cuts at: 513 1065 2940 3084 3697 513 Size: 552 1875 144 613 3885 Fragments arranged by size: 3885 1875 613 552 144 NdeI CA'TA_TG Cuts at: 387 387 Size: 7069 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7069 NotI GC'GGCC_GC Cuts at: 2015 3811 4516 2015 Size: 1796 705 4568 Fragments arranged by size: 4568 1796 705 NsiI A_TGCA'T Cuts at: 2809 2809 Size: 7069 NspI r_CATG'y Cuts at: 1132 1229 2578 3787 4427 5972 1132 Size: 97 1349 1209 640 1545 2229 Fragments arranged by size: 2229 1545 1349 1209 640 97 PasI CC'CwG_GG Cuts at: 1348 3059 1348 Size: 1711 5358 PciI A'CATG_T Cuts at: 2574 5968 2574 Size: 3394 3675 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 2744 2818 1108 Size: 1374 262 74 5359 Fragments arranged by size: 5359 1374 262 74 PmeI GTTT'AAAC Cuts at: 4373 4373 Size: 7069 PmlI CAC'GTG Cuts at: 2659 2659 Size: 7069 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5138 5170 6651 6683 5138 Size: 32 1481 32 5524 Fragments arranged by size: 5524 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3441 3441 Size: 7069 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7069 PsiI TTA'TAA Cuts at: 4639 4639 Size: 7069 PspOMI G'GGCC_C Cuts at: 2084 4150 2084 Size: 2066 5003 PstI C_TGCA'G Cuts at: 830 3569 4421 830 Size: 2739 852 3478 Fragments arranged by size: 3478 2739 852 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7069 PvuII CAG'CTG Cuts at: 3711 3837 3711 Size: 126 6943 SacI G_AGCT'C Cuts at: 2117 3054 2117 Size: 937 6132 SacII CC_GC'GG Cuts at: 2021 3817 2021 Size: 1796 5273 SalI G'TCGA_C Cuts at: 1936 4411 1936 Size: 2475 4594 SapI GCTCTTCn'nnn_ Cuts at: 4125 4125 Size: 7069 SbfI CC_TGCA'GG Cuts at: 4421 4421 Size: 7069 ScaI AGT'ACT Cuts at: 2271 3641 4088 5317 7029 2271 Size: 1370 447 1229 1712 2311 Fragments arranged by size: 2311 1712 1370 1229 447 SexAI A'CCwGG_T Cuts at: 2676 2676 Size: 7069 SfcI C'TryA_G Cuts at: 826 1045 2769 3043 3565 4417 5552 6233 6424 Size: 219 1724 274 522 852 1135 681 191 Cuts at: 6424 826 Size: 1471 Fragments arranged by size: 1724 1471 1135 852 681 522 274 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7069 SmaI CCC'GGG Cuts at: 1872 3548 4396 1872 Size: 1676 848 4545 Fragments arranged by size: 4545 1676 848 SmlI C'TyrA_G Cuts at: 820 1017 1943 2325 3230 3248 4016 5173 6074 Size: 197 926 382 905 18 768 1157 901 Cuts at: 6074 6336 820 Size: 262 1553 Fragments arranged by size: 1553 1157 926 905 901 768 382 262 197 18 SnaBI TAC'GTA Cuts at: 493 4860 493 Size: 4367 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7069 SphI G_CATG'C Cuts at: 1132 4427 1132 Size: 3295 3774 SspI AAT'ATT Cuts at: 5 52 4993 5 Size: 47 4941 2081 Fragments arranged by size: 4941 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7069 StyI C'CwwG_G Cuts at: 513 1065 2940 3084 3285 3697 4146 4508 513 Size: 552 1875 144 201 412 449 362 3074 Fragments arranged by size: 3074 1875 552 449 412 362 201 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2516 4266 5096 5113 5266 5451 5896 Size: 1255 936 1750 830 17 153 185 445 Cuts at: 5896 325 Size: 1498 Fragments arranged by size: 1750 1498 1255 936 830 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2269 3639 4086 Size: 274 80 33 51 1443 292 1370 447 Cuts at: 4086 5315 7027 96 Size: 1229 1712 138 Fragments arranged by size: 1712 1443 1370 1229 447 292 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2340 2372 2450 2482 3249 3281 3710 3742 7029 Size: 32 78 32 767 32 429 32 3287 Cuts at: 7029 7061 2340 Size: 32 2348 Fragments arranged by size: 3287 2348 767 429 78 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4070 1316 Size: 2754 4315 XbaI T'CTAG_A Cuts at: 4405 4405 Size: 7069 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3334 3710 4120 3334 Size: 376 410 6283 Fragments arranged by size: 6283 410 376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7069 XmaI C'CCGG_G Cuts at: 1870 3546 4394 1870 Size: 1676 848 4545 Fragments arranged by size: 4545 1676 848 XmnI GAAnn'nnTTC Cuts at: 2378 5198 2378 Size: 2820 4249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4877 276 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI