(Circular) (Six-base) MAPSORT of: pFN21AB5080.seq Check: 1988 from: 1 to: 6880 pFN21AB5080 6880 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4690 278 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2754 3650 4223 1937 Size: 817 896 573 4594 Fragments arranged by size: 4594 896 817 573 Acc65I G'GTAC_C Cuts at: 4201 4201 Size: 6880 AclI AA'CG_TT Cuts at: 1563 5007 5380 1563 Size: 3444 373 3063 Fragments arranged by size: 3444 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 2913 3572 4943 6327 6595 1632 Size: 965 316 659 1371 1384 268 1917 Fragments arranged by size: 1917 1384 1371 965 659 316 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6683 AflIII A'CryG_T Cuts at: 1203 5779 6756 1203 Size: 4576 977 1327 Fragments arranged by size: 4576 1327 977 AgeI A'CCGG_T Cuts at: 2055 5711 2055 Size: 3656 3224 AhdI GACnn_n'nnGTC Cuts at: 5609 5609 Size: 6880 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6880 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2213 3159 1184 Size: 1029 946 4905 Fragments arranged by size: 4905 1029 946 AlwNI CAG_nnn'CTG Cuts at: 2575 2879 2963 3062 4623 6195 2575 Size: 304 84 99 1561 1572 3260 Fragments arranged by size: 3260 1572 1561 304 99 84 ApaLI G'TGCA_C Cuts at: 3212 3455 4937 6093 3212 Size: 243 1482 1156 3999 Fragments arranged by size: 3999 1482 1156 243 ApoI r'AATT_y Cuts at: 1493 2257 2414 2672 4189 4415 4567 6676 1493 Size: 764 157 258 1517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 764 258 226 157 152 AseI AT'TA_AT Cuts at: 160 1032 5434 160 Size: 872 4402 1606 Fragments arranged by size: 4402 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 AvaI C'yCGr_G Cuts at: 1870 1943 2071 3228 4205 1870 Size: 73 128 1157 977 4545 Fragments arranged by size: 4545 1157 977 128 73 BamHI G'GATC_C Cuts at: 4210 4577 4210 Size: 367 6513 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4201 5656 618 Size: 325 217 627 2414 1455 1842 Fragments arranged by size: 2414 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3929 4199 1391 Size: 2538 270 4072 Fragments arranged by size: 4072 2538 270 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3640 928 Size: 971 1741 4168 Fragments arranged by size: 4168 1741 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5071 5105 1903 Size: 34 3134 34 3678 Fragments arranged by size: 3678 3134 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4772 5982 4772 Size: 1210 5670 BclI T'GATC_A Cuts at: 1548 2791 3051 3598 1548 Size: 1243 260 547 4830 Fragments arranged by size: 4830 1243 547 260 BfrBI ATG'CAT Cuts at: 2266 2266 Size: 6880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5491 136 Size: 107 122 71 631 605 337 3482 1525 Fragments arranged by size: 3482 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6875 6875 Size: 6880 BlpI GC'TnA_GC Cuts at: 4297 4297 Size: 6880 Bme1580I G_kGCm'C Cuts at: 1790 3216 3459 4941 6097 1790 Size: 1426 243 1482 1156 2573 Fragments arranged by size: 2573 1482 1426 1156 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2225 4611 5569 Size: 416 53 83 399 24 808 2386 958 Cuts at: 5569 442 Size: 1753 Fragments arranged by size: 2386 1753 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6880 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4040 Size: 6880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 3279 4017 5540 1239 Size: 1305 735 738 1523 2579 Fragments arranged by size: 2579 1523 1305 738 735 Bpu10I CC'TnA_GC Cuts at: 3952 4113 3952 Size: 161 6719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3539 5005 5870 6168 3539 Size: 1466 865 298 4251 Fragments arranged by size: 4251 1466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3551 5543 882 Size: 617 2052 1992 2219 Fragments arranged by size: 2219 2052 1992 617 BsaAI yAC'GTr Cuts at: 493 1204 4671 493 Size: 711 3467 2702 Fragments arranged by size: 3467 2702 711 BsaBI GATnn'nnATC Cuts at: 6874 6874 Size: 6880 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4687 5069 5704 275 Size: 53 83 186 936 3154 382 635 1451 Fragments arranged by size: 3154 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 5313 5711 5985 6132 6543 2055 Size: 3258 398 274 147 411 2392 Fragments arranged by size: 3258 2392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2517 2547 3264 3294 3990 Size: 30 165 30 1114 30 717 30 696 Cuts at: 3990 4020 1178 Size: 30 4038 Fragments arranged by size: 4038 1114 717 696 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2190 2451 2649 2879 3216 4163 1186 Size: 1004 261 198 230 337 947 3903 Fragments arranged by size: 3903 1004 947 337 261 230 198 BseYI C'CCAG_C Cuts at: 2313 2872 2908 3446 6083 6579 2313 Size: 559 36 538 2637 496 2614 Fragments arranged by size: 2637 2614 559 538 496 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2352 2858 3772 1163 Size: 573 616 506 914 4271 Fragments arranged by size: 4271 914 616 573 506 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4330 5091 6119 1540 Size: 275 186 2329 761 1028 2301 Fragments arranged by size: 2329 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2498 3216 3459 3929 4114 4941 5026 6097 2498 Size: 718 243 470 185 827 85 1071 3281 Fragments arranged by size: 3281 1071 827 718 470 243 185 85 BsmI GAATG_Cn' Cuts at: 4391 4484 4391 Size: 93 6787 BsmBI CGTCTCn'nnnn_ Cuts at: 2072 3267 2072 Size: 1195 5685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2498 3216 3459 3929 4114 4199 4941 Size: 399 708 718 243 470 185 85 742 Cuts at: 4941 5026 6097 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 718 708 470 399 243 185 85 85 BspEI T'CCGG_A Cuts at: 6543 6543 Size: 6880 BspHI T'CATG_A Cuts at: 2610 4769 6499 2610 Size: 2159 1730 2991 Fragments arranged by size: 2991 2159 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4235 844 Size: 3391 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2306 4767 1394 Size: 429 483 2461 3507 Fragments arranged by size: 3507 2461 483 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2163 2809 5375 5549 65 Size: 1183 915 646 2566 174 1396 Fragments arranged by size: 2566 1396 1183 915 646 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2055 5524 5711 1700 Size: 110 245 3469 187 2869 Fragments arranged by size: 3469 2869 245 187 110 BsrGI T'GTAC_A Cuts at: 96 2221 96 Size: 2125 4755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6880 BssSI C'ACGA_G Cuts at: 4940 5952 4940 Size: 1012 5868 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6880 BstBI TT'CG_AA Cuts at: 3150 5684 3150 Size: 2534 4346 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6880 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2786 3761 4048 2215 Size: 571 975 287 5047 Fragments arranged by size: 5047 975 571 287 BstYI r'GATC_y Cuts at: 1972 3172 4210 4577 4962 4979 6431 6517 6529 Size: 1200 1038 367 385 17 1452 86 12 Cuts at: 6529 6875 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1200 1038 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3651 3651 Size: 6880 BtgI C'CryG_G Cuts at: 513 1065 2004 2797 513 Size: 552 939 793 4596 Fragments arranged by size: 4596 939 793 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6141 Fragments arranged by size: 6141 683 56 BtsI GCAGTG_nn' Cuts at: 3631 4396 5190 5210 3631 Size: 765 794 20 5301 Fragments arranged by size: 5301 794 765 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3232 3267 3385 3420 529 Size: 35 2668 35 118 35 3989 Fragments arranged by size: 3989 2668 118 35 35 35 DraI TTT'AAA Cuts at: 4184 4184 Size: 6880 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3899 5887 809 Size: 507 2583 1988 1802 Fragments arranged by size: 2583 1988 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 3427 4327 Size: 54 1425 50 275 18 522 1075 900 Cuts at: 4327 5216 6845 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1075 900 889 522 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4327 1537 Size: 275 2515 4090 Fragments arranged by size: 4090 2515 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3591 4810 1353 Size: 2238 1219 3423 Fragments arranged by size: 3423 2238 1219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2060 5467 5841 5987 387 Size: 1064 138 471 3407 374 146 1280 Fragments arranged by size: 3407 1280 1064 471 374 146 138 EcoICRI GAG'CTC Cuts at: 3927 3927 Size: 6880 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2544 2597 2913 3279 3572 4017 4943 Size: 393 912 53 316 366 293 445 926 Cuts at: 4943 5540 6327 6595 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 926 912 787 597 445 393 366 316 293 268 53 EcoNI CCTnn'n_nnAGG Cuts at: 3198 3198 Size: 6880 EcoO109I rG'GnC_Cy Cuts at: 2158 2456 2869 2158 Size: 298 413 6169 Fragments arranged by size: 6169 413 298 EcoRI G'AATT_C Cuts at: 4189 4567 4189 Size: 378 6502 FspI TGC'GCA Cuts at: 2201 5386 2201 Size: 3185 3695 FspAI rTGC'GCAy Cuts at: 2201 2201 Size: 6880 HaeII r_GCGC'y Cuts at: 1678 2879 6027 1678 Size: 1201 3148 2531 Fragments arranged by size: 3148 2531 1201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 2833 2865 3590 Size: 32 135 32 974 32 255 32 725 Cuts at: 3590 3622 3627 3659 4142 4174 5521 5553 5595 Size: 32 5 32 483 32 1347 32 42 Cuts at: 5595 5627 1373 Size: 32 2626 Fragments arranged by size: 2626 1347 974 725 483 255 135 42 32 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 4224 4470 669 Size: 988 281 2286 246 3079 Fragments arranged by size: 3079 2286 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6880 HpaI GTT'AAC Cuts at: 4470 4470 Size: 6880 KpnI G_GTAC'C Cuts at: 4205 4205 Size: 6880 MfeI C'AATT_G Cuts at: 4479 4479 Size: 6880 MluI A'CGCG_T Cuts at: 6756 6756 Size: 6880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2932 3367 3482 5994 6178 1305 Size: 1627 435 115 2512 184 2007 Fragments arranged by size: 2512 2007 1627 435 184 115 MscI TGG'CCA Cuts at: 10 64 1489 1832 2354 3429 10 Size: 54 1425 343 522 1075 3461 Fragments arranged by size: 3461 1425 1075 522 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2101 2348 2802 3008 3194 4031 4046 Size: 648 935 247 454 206 186 837 15 Cuts at: 4046 4838 5197 5356 518 Size: 792 359 159 2042 Fragments arranged by size: 2042 935 837 792 648 454 359 247 206 186 159 15 MspA1I CmG'CkG Cuts at: 1606 4296 4973 6121 6366 1606 Size: 2690 677 1148 245 2120 Fragments arranged by size: 2690 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2797 513 Size: 552 939 793 4596 Fragments arranged by size: 4596 939 793 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6880 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6880 NotI GC'GGCC_GC Cuts at: 4327 4327 Size: 6880 NsiI A_TGCA'T Cuts at: 2268 2268 Size: 6880 NspI r_CATG'y Cuts at: 1132 1229 2270 2771 3733 4238 5783 1132 Size: 97 1041 501 962 505 1545 2229 Fragments arranged by size: 2229 1545 1041 962 505 501 97 PasI CC'CwG_GG Cuts at: 1348 3541 1348 Size: 2193 4687 PciI A'CATG_T Cuts at: 5779 5779 Size: 6880 PflMI CCAn_nnn'nTGG Cuts at: 1108 2879 4076 1108 Size: 1771 1197 3912 Fragments arranged by size: 3912 1771 1197 PfoI T'CCnGG_A Cuts at: 2383 3843 2383 Size: 1460 5420 PmeI GTTT'AAAC Cuts at: 4184 4184 Size: 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4949 4981 6462 6494 4949 Size: 32 1481 32 5335 Fragments arranged by size: 5335 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2158 Size: 6880 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6880 PsiI TTA'TAA Cuts at: 4450 4450 Size: 6880 PspXI vC'TCGA_Gb Cuts at: 3228 3228 Size: 6880 PstI C_TGCA'G Cuts at: 830 2192 2923 3138 3514 4232 830 Size: 1362 731 215 376 718 3478 Fragments arranged by size: 3478 1362 731 718 376 215 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6880 SacI G_AGCT'C Cuts at: 3929 3929 Size: 6880 SalI G'TCGA_C Cuts at: 1936 4222 1936 Size: 2286 4594 SbfI CC_TGCA'GG Cuts at: 2192 4232 2192 Size: 2040 4840 ScaI AGT'ACT Cuts at: 5128 6840 5128 Size: 1712 5168 SfcI C'TryA_G Cuts at: 826 1045 2188 2830 2919 3134 3510 4099 4228 Size: 219 1143 642 89 215 376 589 129 Cuts at: 4228 5363 6044 6235 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1143 1135 681 642 589 376 219 215 191 129 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6880 SmaI CCC'GGG Cuts at: 1872 4207 1872 Size: 2335 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3228 3554 4984 5885 6147 820 Size: 197 926 1285 326 1430 901 262 1553 Fragments arranged by size: 1553 1430 1285 926 901 326 262 197 SnaBI TAC'GTA Cuts at: 493 4671 493 Size: 4178 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6880 SphI G_CATG'C Cuts at: 1132 3733 4238 1132 Size: 2601 505 3774 Fragments arranged by size: 3774 2601 505 SspI AAT'ATT Cuts at: 5 52 3382 4804 5 Size: 47 3330 1422 2081 Fragments arranged by size: 3330 2081 1422 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6880 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 2797 3760 3870 4319 513 Size: 552 939 150 643 963 110 449 3074 Fragments arranged by size: 3074 963 939 643 552 449 150 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2089 2207 3447 3587 4907 4924 5077 Size: 1255 509 118 1240 140 1320 17 153 Cuts at: 5077 5262 5707 325 Size: 185 445 1498 Fragments arranged by size: 1498 1320 1255 1240 509 445 185 153 140 118 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2221 2811 Size: 274 80 33 51 1443 147 97 590 Cuts at: 2811 3375 5126 6838 96 Size: 564 1751 1712 138 Fragments arranged by size: 1751 1712 1443 590 564 274 147 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2361 2393 3990 4022 6840 6872 2361 Size: 32 1597 32 2818 32 2369 Fragments arranged by size: 2818 2369 1597 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6880 XbaI T'CTAG_A Cuts at: 4216 4216 Size: 6880 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 2509 Size: 6880 XhoI C'TCGA_G Cuts at: 1943 3228 1943 Size: 1285 5595 XmaI C'CCGG_G Cuts at: 1870 4205 1870 Size: 2335 4545 XmnI GAAnn'nnTTC Cuts at: 2717 4094 5009 2717 Size: 1377 915 4588 Fragments arranged by size: 4588 1377 915 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4688 276 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI Bsu36I DraIII EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI