Restriction map for
pFN21AB8685
(Linear) (Six-base) MAPSORT of: KIBB8685.seq Check: 9755 from: 1 to: 2151
KIBB8685.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 0 1604 2151
Size: 1604 547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 502 878 2151
Size: 494 8 376 1273
Fragments arranged by size:
1273 494 376 8
AhdI GACnn_n'nnGTC
Cuts at: 0 708 2151
Size: 708 1443
AleI CACnn'nnGTG
Cuts at: 0 554 1666 2151
Size: 554 1112 485
Fragments arranged by size:
1112 554 485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1606 2151
Size: 1606 545
AlwNI CAG_nnn'CTG
Cuts at: 0 105 399 1844 2064 2151
Size: 105 294 1445 220 87
Fragments arranged by size:
1445 294 220 105 87
ApaI G_GGCC'C
Cuts at: 0 1449 2151
Size: 1449 702
ApoI r'AATT_y
Cuts at: 0 1081 2151
Size: 1081 1070
AvaI C'yCGr_G
Cuts at: 0 118 574 787 1419 1888 1960 2151
Size: 118 456 213 632 469 72 191
Fragments arranged by size:
632 469 456 213 191 118 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1987 2020 2151
Size: 1987 33 131
Fragments arranged by size:
1987 131 33
BanI G'GyrC_C
Cuts at: 0 478 564 1264 1812 2151
Size: 478 86 700 548 339
Fragments arranged by size:
700 548 478 339 86
BanII G_rGCy'C
Cuts at: 0 341 422 575 958 1449 2151
Size: 341 81 153 383 491 702
Fragments arranged by size:
702 491 383 341 153 81
BbeI G_GCGC'C
Cuts at: 0 1268 2151
Size: 1268 883
BbsI GAAGACnn'nnnn_
Cuts at: 0 213 1690 2151
Size: 213 1477 461
Fragments arranged by size:
1477 461 213
BbvCI CC'TCA_GC
Cuts at: 0 230 299 643 2151
Size: 230 69 344 1508
Fragments arranged by size:
1508 344 230 69
BglI GCCn_nnn'nGGC
Cuts at: 0 1262 1490 2151
Size: 1262 228 661
Fragments arranged by size:
1262 661 228
Bme1580I G_kGCm'C
Cuts at: 0 483 561 905 1449 2151
Size: 483 78 344 544 702
Fragments arranged by size:
702 544 483 344 78
BmgBI CAC'GTC
Cuts at: 0 1329 2151
Size: 1329 822
BmrI ACTGGGnnnn_n'
Cuts at: 0 418 1629 2003 2151
Size: 418 1211 374 148
Fragments arranged by size:
1211 418 374 148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 77 1275 2151
Size: 77 1198 876
Fragments arranged by size:
1198 876 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 352 604 739 1319 1615 2151
Size: 125 227 252 135 580 296 536
Fragments arranged by size:
580 536 296 252 227 135 125
Bpu10I CC'TnA_GC
Cuts at: 0 23 98 230 299 569 643 952 1260
Size: 23 75 132 69 270 74 309 308
Cuts at: 1260 2151
Size: 891
Fragments arranged by size:
891 309 308 270 132 75 74 69
23
BsaHI Gr'CG_yC
Cuts at: 0 146 944 1265 2151
Size: 146 798 321 886
Fragments arranged by size:
886 798 321 146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 892 895 1111 1114 1123 1126 1129
Size: 136 756 3 216 3 9 3 3
Cuts at: 1129 1138 1180 1183 1192 1201 1264 1525 1528
Size: 9 42 3 9 9 63 261 3
Cuts at: 1528 1540 1543 1576 1705 1708 1711 1726 1756
Size: 12 3 33 129 3 3 15 30
Cuts at: 1756 2068 2116 2119 2122 2125 2134 2137 2140
Size: 312 48 3 3 3 9 3 3
Cuts at: 2140 2143 2151
Size: 3 8
Fragments arranged by size:
756 312 261 216 136 129 63 48
42 33 30 15 12 9 9 9
9 9 8 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 35 149 243 482 537 1488 1543 1940
Size: 35 114 94 239 55 951 55 397
Cuts at: 1940 2151
Size: 211
Fragments arranged by size:
951 397 239 211 114 94 55 55
35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1644 2151
Size: 616 1028 507
Fragments arranged by size:
1028 616 507
BsiEI CG_ry'CG
Cuts at: 0 1469 2151
Size: 1469 682
BsiHKAI G_wGCw'C
Cuts at: 0 341 659 958 2151
Size: 341 318 299 1193
Fragments arranged by size:
1193 341 318 299
Bsp1286I G_dGCh'C
Cuts at: 0 341 422 483 561 575 659 905 958
Size: 341 81 61 78 14 84 246 53
Cuts at: 958 1449 2151
Size: 491 702
Fragments arranged by size:
702 491 341 246 84 81 78 61
53 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 318 623 1448 1467 2151
Size: 17 301 305 825 19 684
Fragments arranged by size:
825 684 305 301 19 17
BsrBI CCG'CTC
Cuts at: 0 1259 2151
Size: 1259 892
BsrFI r'CCGG_y
Cuts at: 0 940 2151
Size: 940 1211
BstAPI GCAn_nnn'nTGC
Cuts at: 0 635 1644 1844 2151
Size: 635 1009 200 307
Fragments arranged by size:
1009 635 307 200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 2151
Size: 912 1239
BstYI r'GATC_y
Cuts at: 0 680 1040 2151
Size: 680 360 1111
Fragments arranged by size:
1111 680 360
Bsu36I CC'TnA_GG
Cuts at: 0 698 2151
Size: 698 1453
BtgI C'CryG_G
Cuts at: 0 1196 1309 1610 2151
Size: 1196 113 301 541
Fragments arranged by size:
1196 541 301 113
BtsI GCAGTG_nn'
Cuts at: 0 748 1768 2151
Size: 748 1020 383
Fragments arranged by size:
1020 748 383
DraIII CAC_nnn'GTG
Cuts at: 0 743 2151
Size: 743 1408
DrdI GACnn_nn'nnGTC
Cuts at: 0 1396 2151
Size: 1396 755
EaeI y'GGCC_r
Cuts at: 0 590 983 1070 1466 1613 1862 2151
Size: 590 393 87 396 147 249 289
Fragments arranged by size:
590 396 393 289 249 147 87
EagI C'GGCC_G
Cuts at: 0 1466 2151
Size: 1466 685
EarI CTCTTCn'nnn_
Cuts at: 0 1712 2045 2151
Size: 1712 333 106
Fragments arranged by size:
1712 333 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 829 2151
Size: 829 1322
EcoICRI GAG'CTC
Cuts at: 0 339 956 2151
Size: 339 617 1195
Fragments arranged by size:
1195 617 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 352 494 502 604 739 878 1319
Size: 125 227 142 8 102 135 139 441
Cuts at: 1319 1615 2151
Size: 296 536
Fragments arranged by size:
536 441 296 227 142 139 135 125
102 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 72 1648 1708 2151
Size: 72 1576 60 443
Fragments arranged by size:
1576 443 72 60
EcoO109I rG'GnC_Cy
Cuts at: 0 102 702 1701 1892 2151
Size: 102 600 999 191 259
Fragments arranged by size:
999 600 259 191 102
EcoRI G'AATT_C
Cuts at: 0 1081 2151
Size: 1081 1070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1389 2151
Size: 1389 762
HaeII r_GCGC'y
Cuts at: 0 30 832 1268 2151
Size: 30 802 436 883
Fragments arranged by size:
883 802 436 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 454 486 694 726 2151
Size: 247 32 175 32 208 32 1425
Fragments arranged by size:
1425 247 208 175 32 32 32
HincII GTy'rAC
Cuts at: 0 429 1605 2151
Size: 429 1176 546
Fragments arranged by size:
1176 546 429
KasI G'GCGC_C
Cuts at: 0 1264 2151
Size: 1264 887
MfeI C'AATT_G
Cuts at: 0 633 2151
Size: 633 1518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1901 2151
Size: 1901 250
MscI TGG'CCA
Cuts at: 0 985 1615 2151
Size: 985 630 536
Fragments arranged by size:
985 630 536
MslI CAynn'nnrTG
Cuts at: 0 554 1588 1666 2029 2151
Size: 554 1034 78 363 122
Fragments arranged by size:
1034 554 363 122 78
MspA1I CmG'CkG
Cuts at: 0 112 363 693 996 1488 1658 2151
Size: 112 251 330 303 492 170 493
Fragments arranged by size:
493 492 330 303 251 170 112
NaeI GCC'GGC
Cuts at: 0 942 2151
Size: 942 1209
NarI GG'CG_CC
Cuts at: 0 1265 2151
Size: 1265 886
NcoI C'CATG_G
Cuts at: 0 1309 1610 2151
Size: 1309 301 541
Fragments arranged by size:
1309 541 301
NgoMIV G'CCGG_C
Cuts at: 0 940 2151
Size: 940 1211
PasI CC'CwG_GG
Cuts at: 0 2088 2151
Size: 2088 63
PflMI CCAn_nnn'nTGG
Cuts at: 0 399 743 1883 2151
Size: 399 344 1140 268
Fragments arranged by size:
1140 399 344 268
PfoI T'CCnGG_A
Cuts at: 0 413 716 1085 2151
Size: 413 303 369 1066
Fragments arranged by size:
1066 413 369 303
PpuMI rG'GwC_Cy
Cuts at: 0 702 1701 2151
Size: 702 999 450
Fragments arranged by size:
999 702 450
PspOMI G'GGCC_C
Cuts at: 0 1445 2151
Size: 1445 706
PstI C_TGCA'G
Cuts at: 0 362 623 1840 2151
Size: 362 261 1217 311
Fragments arranged by size:
1217 362 311 261
PvuII CAG'CTG
Cuts at: 0 996 2151
Size: 996 1155
SacI G_AGCT'C
Cuts at: 0 341 958 2151
Size: 341 617 1193
Fragments arranged by size:
1193 617 341
SalI G'TCGA_C
Cuts at: 0 1603 2151
Size: 1603 548
ScaI AGT'ACT
Cuts at: 0 1855 2151
Size: 1855 296
SexAI A'CCwGG_T
Cuts at: 0 659 1064 2151
Size: 659 405 1087
Fragments arranged by size:
1087 659 405
SfcI C'TryA_G
Cuts at: 0 358 552 619 1836 2151
Size: 358 194 67 1217 315
Fragments arranged by size:
1217 358 315 194 67
SfoI GGC'GCC
Cuts at: 0 1266 2151
Size: 1266 885
SmaI CCC'GGG
Cuts at: 0 1421 1890 2151
Size: 1421 469 261
Fragments arranged by size:
1421 469 261
StuI AGG'CCT
Cuts at: 0 67 610 2151
Size: 67 543 1541
Fragments arranged by size:
1541 543 67
StyI C'CwwG_G
Cuts at: 0 434 605 1244 1309 1610 2151
Size: 434 171 639 65 301 541
Fragments arranged by size:
639 541 434 301 171 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 1611 1656 2151
Size: 235 1376 45 495
Fragments arranged by size:
1376 495 235 45
TatI w'GTAC_w
Cuts at: 0 1280 1853 1952 2151
Size: 1280 573 99 199
Fragments arranged by size:
1280 573 199 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 264 296 1625 1657 2151
Size: 264 32 1329 32 494
Fragments arranged by size:
1329 494 264 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1204 2151
Size: 1204 947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 807 993 2151
Size: 807 186 1158
Fragments arranged by size:
1158 807 186
XmaI C'CCGG_G
Cuts at: 0 1419 1888 2151
Size: 1419 469 263
Fragments arranged by size:
1419 469 263
XmnI GAAnn'nnTTC
Cuts at: 0 1372 2151
Size: 1372 779
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SalI
ScaI SexAI SfcI SfoI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MluI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SanDI SapI
SbfI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI ZraI