(Circular) (Six-base) MAPSORT of: pFN21AB3755.seq Check: 9017 from: 1 to: 6475 pFN21AB3755 6475 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3163 3163 Size: 6475 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3333 4285 278 Size: 53 83 186 936 1797 952 2468 Fragments arranged by size: 2468 1797 952 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 2165 3796 2165 Size: 1631 4844 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 3431 4538 5922 6190 1632 Size: 689 1110 1107 1384 268 1917 Fragments arranged by size: 1917 1384 1110 1107 689 268 AflII C'TTAA_G Cuts at: 820 1017 2326 820 Size: 197 1309 4969 Fragments arranged by size: 4969 1309 197 AflIII A'CryG_T Cuts at: 1203 5374 6351 1203 Size: 4171 977 1327 Fragments arranged by size: 4171 1327 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 3420 3737 5204 3420 Size: 317 1467 4691 Fragments arranged by size: 4691 1467 317 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6475 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2638 2670 3038 3070 2638 Size: 32 368 32 6043 Fragments arranged by size: 6043 368 32 32 AlwNI CAG_nnn'CTG Cuts at: 4218 5790 4218 Size: 1572 4903 ApaLI G'TGCA_C Cuts at: 4532 5688 4532 Size: 1156 5319 ApoI r'AATT_y Cuts at: 1493 3784 4010 4162 6271 1493 Size: 2291 226 152 2109 1697 Fragments arranged by size: 2291 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2133 3761 3800 1870 Size: 73 152 38 1628 39 4545 Fragments arranged by size: 4545 1628 152 73 39 38 BamHI G'GATC_C Cuts at: 3805 4172 3805 Size: 367 6108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 3175 3796 5251 618 Size: 325 217 627 378 1010 621 1455 1842 Fragments arranged by size: 1842 1455 1010 627 621 378 325 217 BanII G_rGCy'C Cuts at: 1391 2608 2691 3794 1391 Size: 1217 83 1103 4072 Fragments arranged by size: 4072 1217 1103 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2203 2309 3419 928 Size: 971 304 106 1110 3984 Fragments arranged by size: 3984 1110 971 304 106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2695 2729 4666 4700 1903 Size: 34 758 34 1937 34 3678 Fragments arranged by size: 3678 1937 758 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2058 4367 5577 2058 Size: 2309 1210 2956 Fragments arranged by size: 2956 2309 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3392 5086 136 Size: 107 122 71 631 605 1720 1694 1525 Fragments arranged by size: 1720 1694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6470 6470 Size: 6475 BlpI GC'TnA_GC Cuts at: 3892 3892 Size: 6475 Bme1580I G_kGCm'C Cuts at: 1790 4536 5692 1790 Size: 2746 1156 2573 Fragments arranged by size: 2746 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4206 5164 442 Size: 416 53 83 399 24 2789 958 1753 Fragments arranged by size: 2789 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3423 3423 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2779 3062 5135 1239 Size: 903 637 283 2073 2579 Fragments arranged by size: 2579 2073 903 637 283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3095 4600 5465 5763 3095 Size: 1505 865 298 3807 Fragments arranged by size: 3807 1505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5138 882 Size: 617 3639 2219 Fragments arranged by size: 3639 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2452 2529 4266 493 Size: 711 1248 77 1737 2702 Fragments arranged by size: 2702 1737 1248 711 77 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3330 4282 4664 5299 Size: 53 83 186 936 1797 952 382 635 Cuts at: 5299 275 Size: 1451 Fragments arranged by size: 1797 1451 952 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4908 5306 5580 5727 6138 4908 Size: 398 274 147 411 5245 Fragments arranged by size: 5245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2060 2069 2090 2638 Size: 30 165 30 636 21 9 21 548 Cuts at: 2638 2668 3038 3062 3068 3092 3320 3350 1178 Size: 30 370 24 6 24 228 30 4303 Fragments arranged by size: 4303 636 548 370 228 165 30 30 30 30 24 24 21 21 9 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2143 3046 3080 3197 3200 3328 1186 Size: 861 96 903 34 117 3 128 4333 Fragments arranged by size: 4333 903 861 128 117 96 34 3 BseYI C'CCAG_C Cuts at: 2969 3190 5678 6174 2969 Size: 221 2488 496 3270 Fragments arranged by size: 3270 2488 496 221 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5902 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3925 4686 5714 1540 Size: 275 186 1924 761 1028 2301 Fragments arranged by size: 2301 1924 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3111 3356 4536 4621 5692 3111 Size: 245 1180 85 1071 3894 Fragments arranged by size: 3894 1180 1071 245 85 BsmI GAATG_Cn' Cuts at: 3986 4079 3986 Size: 93 6382 BsmBI CGTCTCn'nnnn_ Cuts at: 3077 3077 Size: 6475 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2608 2691 3111 3356 3794 4536 4621 Size: 399 818 83 420 245 438 742 85 Cuts at: 4621 5692 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 818 742 438 420 399 245 85 83 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 2394 4364 6094 2394 Size: 1970 1730 2775 Fragments arranged by size: 2775 1970 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3163 3177 3830 844 Size: 2319 14 653 3489 Fragments arranged by size: 3489 2319 653 14 BsrBI CCG'CTC Cuts at: 1394 1823 2524 4362 1394 Size: 429 701 1838 3507 Fragments arranged by size: 3507 1838 701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 4970 5144 65 Size: 1183 1219 2503 174 1396 Fragments arranged by size: 2503 1396 1219 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2558 5119 5306 1700 Size: 110 748 2561 187 2869 Fragments arranged by size: 2869 2561 748 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 3754 1925 Size: 1829 4646 BssSI C'ACGA_G Cuts at: 2515 4535 5547 2515 Size: 2020 1012 3443 Fragments arranged by size: 3443 2020 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 2245 1184 Size: 1061 5414 BstYI r'GATC_y Cuts at: 1972 2763 3805 4172 4557 4574 6026 6112 6124 Size: 791 1042 367 385 17 1452 86 12 Cuts at: 6124 6470 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1042 791 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2410 3180 2410 Size: 770 5705 BtgI C'CryG_G Cuts at: 513 1065 2853 3306 513 Size: 552 1788 453 3682 Fragments arranged by size: 3682 1788 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2200 2698 487 Size: 683 56 974 498 4264 Fragments arranged by size: 4264 974 683 498 56 BtsI GCAGTG_nn' Cuts at: 3010 3991 4785 4805 3010 Size: 981 794 20 4680 Fragments arranged by size: 4680 981 794 20 ClaI AT'CG_AT Cuts at: 1554 2401 1554 Size: 847 5628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5482 809 Size: 507 4166 1802 Fragments arranged by size: 4166 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2929 3357 3393 Size: 54 1425 50 275 18 1099 428 36 Cuts at: 3393 3922 4811 6440 8 Size: 529 889 1629 43 Fragments arranged by size: 1629 1425 1099 889 529 428 275 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3922 1537 Size: 275 2110 4090 Fragments arranged by size: 4090 2110 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3233 4405 1353 Size: 1880 1172 3423 Fragments arranged by size: 3423 1880 1172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3040 5062 5436 5582 387 Size: 1064 138 1451 2022 374 146 1280 Fragments arranged by size: 2022 1451 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2321 2779 3062 3431 4538 5135 Size: 393 510 179 458 283 369 1107 597 Cuts at: 5135 5922 6190 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1107 787 597 510 458 393 369 283 268 179 EcoNI CCTnn'n_nnAGG Cuts at: 2184 3455 2184 Size: 1271 5204 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 5622 1678 Size: 3944 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2557 2589 3062 3094 3320 Size: 32 135 32 985 32 473 32 226 Cuts at: 3320 3352 3406 3438 3723 3755 5116 5148 5190 Size: 32 54 32 285 32 1361 32 42 Cuts at: 5190 5222 1373 Size: 32 2626 Fragments arranged by size: 2626 1361 985 473 285 226 135 54 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2290 2311 3334 3819 4065 669 Size: 988 281 352 21 1023 485 246 3079 Fragments arranged by size: 3079 1023 988 485 352 281 246 21 HindIII A'AGCT_T Cuts at: 748 3113 3271 3281 748 Size: 2365 158 10 3942 Fragments arranged by size: 3942 2365 158 10 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KpnI G_GTAC'C Cuts at: 2169 3800 2169 Size: 1631 4844 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2617 2937 2993 3084 5589 5773 1305 Size: 1312 320 56 91 2505 184 2007 Fragments arranged by size: 2505 2007 1312 320 184 91 56 MscI TGG'CCA Cuts at: 10 64 1489 1832 3395 10 Size: 54 1425 343 1563 3090 Fragments arranged by size: 3090 1563 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2366 2393 2741 4433 4792 4951 518 Size: 648 1200 27 348 1692 359 159 2042 Fragments arranged by size: 2042 1692 1200 648 359 348 159 27 MspA1I CmG'CkG Cuts at: 1606 2141 2299 2934 3561 3891 4568 5716 5961 Size: 535 158 635 627 330 677 1148 245 Cuts at: 5961 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 635 627 535 330 245 158 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5923 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 3833 5378 1132 Size: 97 2604 1545 2229 Fragments arranged by size: 2604 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2961 1108 Size: 1853 4622 PfoI T'CCnGG_A Cuts at: 2060 2656 2060 Size: 596 5879 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2124 2638 2670 3038 3070 4544 4576 6057 Size: 32 514 32 368 32 1474 32 1481 Cuts at: 6057 6089 2092 Size: 32 2478 Fragments arranged by size: 2478 1481 1474 514 368 32 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PstI C_TGCA'G Cuts at: 830 2876 3174 3231 3827 830 Size: 2046 298 57 596 3478 Fragments arranged by size: 3478 2046 596 298 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2141 2299 3561 2141 Size: 158 1262 5055 Fragments arranged by size: 5055 1262 158 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SapI GCTCTTCn'nnn_ Cuts at: 3233 3233 Size: 6475 SbfI CC_TGCA'GG Cuts at: 3174 3827 3174 Size: 653 5822 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 2872 3170 3227 3823 4958 5639 5830 Size: 219 1827 298 57 596 1135 681 191 Cuts at: 5830 826 Size: 1471 Fragments arranged by size: 1827 1471 1135 681 596 298 219 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 2097 3802 1872 Size: 225 1705 4545 Fragments arranged by size: 4545 1705 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 2133 2326 3110 4579 5480 5742 Size: 197 926 190 193 784 1469 901 262 Cuts at: 5742 820 Size: 1553 Fragments arranged by size: 1553 1469 926 901 784 262 197 193 190 SnaBI TAC'GTA Cuts at: 493 2452 4266 493 Size: 1959 1814 2702 Fragments arranged by size: 2702 1959 1814 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 3770 1517 Size: 2253 4222 StyI C'CwwG_G Cuts at: 513 1065 2241 3771 3914 513 Size: 552 1176 1530 143 3074 Fragments arranged by size: 3074 1530 1176 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2561 3727 4502 4519 4672 4857 5302 Size: 1255 981 1166 775 17 153 185 445 Cuts at: 5302 325 Size: 1498 Fragments arranged by size: 1498 1255 1166 981 775 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2024 4721 6433 Size: 274 80 33 51 1443 47 2697 1712 Cuts at: 6433 96 Size: 138 Fragments arranged by size: 2697 1712 1443 274 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 6435 6467 2060 Size: 32 4343 32 2068 Fragments arranged by size: 4343 2068 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 2133 1943 Size: 190 6285 XmaI C'CCGG_G Cuts at: 1870 2095 3800 1870 Size: 225 1705 4545 Fragments arranged by size: 4545 1705 225 XmnI GAAnn'nnTTC Cuts at: 4604 4604 Size: 6475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3331 4283 276 Size: 53 83 186 936 1797 952 2468 Fragments arranged by size: 2468 1797 952 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI