Restriction map for
pFN21AB3755
(Linear) (Six-base) MAPSORT of: KIBB3755.seq Check: 2140 from: 1 to: 1770
KIBB3755.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1158 1770
Size: 1158 612
AatII G_ACGT'C
Cuts at: 0 1328 1770
Size: 1328 442
Acc65I G'GTAC_C
Cuts at: 0 160 1770
Size: 160 1610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1426 1770
Size: 316 1110 344
Fragments arranged by size:
1110 344 316
AflII C'TTAA_G
Cuts at: 0 321 1770
Size: 321 1449
AhdI GACnn_n'nnGTC
Cuts at: 0 1415 1732 1770
Size: 1415 317 38
Fragments arranged by size:
1415 317 38
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 633 665 1033 1065 1770
Size: 633 32 368 32 705
Fragments arranged by size:
705 633 368 32 32
AvaI C'yCGr_G
Cuts at: 0 90 128 1756 1770
Size: 90 38 1628 14
Fragments arranged by size:
1628 90 38 14
BanI G'GyrC_C
Cuts at: 0 160 1170 1770
Size: 160 1010 600
Fragments arranged by size:
1010 600 160
BanII G_rGCy'C
Cuts at: 0 603 686 1770
Size: 603 83 1084
Fragments arranged by size:
1084 603 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 198 304 1414 1770
Size: 198 106 1110 356
Fragments arranged by size:
1110 356 198 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 690 724 1770
Size: 690 34 1046
Fragments arranged by size:
1046 690 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 53 1770
Size: 53 1717
BglI GCCn_nnn'nGGC
Cuts at: 0 1387 1770
Size: 1387 383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1418 1770
Size: 1418 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 774 1057 1770
Size: 137 637 283 713
Fragments arranged by size:
713 637 283 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1770
Size: 1090 680
BsaAI yAC'GTr
Cuts at: 0 447 524 1770
Size: 447 77 1246
Fragments arranged by size:
1246 447 77
BsaHI Gr'CG_yC
Cuts at: 0 1325 1770
Size: 1325 445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 55 64 85 633 663 1033 1057
Size: 34 21 9 21 548 30 370 24
Cuts at: 1057 1063 1087 1315 1345 1770
Size: 6 24 228 30 425
Fragments arranged by size:
548 425 370 228 34 30 30 24
24 21 21 9 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 138 1041 1075 1192 1195 1323 1770
Size: 42 96 903 34 117 3 128 447
Fragments arranged by size:
903 447 128 117 96 42 34 3
BseYI C'CCAG_C
Cuts at: 0 964 1185 1770
Size: 964 221 585
Fragments arranged by size:
964 585 221
BsiHKAI G_wGCw'C
Cuts at: 0 1106 1351 1770
Size: 1106 245 419
Fragments arranged by size:
1106 419 245
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1072 1770
Size: 1072 698
Bsp1286I G_dGCh'C
Cuts at: 0 603 686 1106 1351 1770
Size: 603 83 420 245 419
Fragments arranged by size:
603 420 419 245 83
BspHI T'CATG_A
Cuts at: 0 389 1770
Size: 389 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1158 1172 1770
Size: 1158 14 598
Fragments arranged by size:
1158 598 14
BsrBI CCG'CTC
Cuts at: 0 519 1770
Size: 519 1251
BsrDI GCAATG_nn'
Cuts at: 0 462 1770
Size: 462 1308
BsrFI r'CCGG_y
Cuts at: 0 553 1770
Size: 553 1217
BssHII G'CGCG_C
Cuts at: 0 1749 1770
Size: 1749 21
BssSI C'ACGA_G
Cuts at: 0 510 1770
Size: 510 1260
BstEII G'GTnAC_C
Cuts at: 0 240 1770
Size: 240 1530
BstYI r'GATC_y
Cuts at: 0 758 1770
Size: 758 1012
Bsu36I CC'TnA_GG
Cuts at: 0 405 1175 1770
Size: 405 770 595
Fragments arranged by size:
770 595 405
BtgI C'CryG_G
Cuts at: 0 848 1301 1770
Size: 848 453 469
Fragments arranged by size:
848 469 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 195 693 1770
Size: 195 498 1077
Fragments arranged by size:
1077 498 195
BtsI GCAGTG_nn'
Cuts at: 0 1005 1770
Size: 1005 765
ClaI AT'CG_AT
Cuts at: 0 396 1770
Size: 396 1374
EaeI y'GGCC_r
Cuts at: 0 924 1352 1388 1770
Size: 924 428 36 382
Fragments arranged by size:
924 428 382 36
EarI CTCTTCn'nnn_
Cuts at: 0 1228 1770
Size: 1228 542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1035 1770
Size: 1035 735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 316 774 1057 1426 1770
Size: 137 179 458 283 369 344
Fragments arranged by size:
458 369 344 283 179 137
EcoNI CCTnn'n_nnAGG
Cuts at: 0 179 1450 1770
Size: 179 1271 320
Fragments arranged by size:
1271 320 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 552 584 1057 1089 1315 1347 1401 1433
Size: 552 32 473 32 226 32 54 32
Cuts at: 1433 1718 1750 1770
Size: 285 32 20
Fragments arranged by size:
552 473 285 226 54 32 32 32
32 32 20
HincII GTy'rAC
Cuts at: 0 285 306 1329 1770
Size: 285 21 1023 441
Fragments arranged by size:
1023 441 285 21
HindIII A'AGCT_T
Cuts at: 0 1108 1266 1276 1770
Size: 1108 158 10 494
Fragments arranged by size:
1108 494 158 10
KpnI G_GTAC'C
Cuts at: 0 164 1770
Size: 164 1606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 932 988 1079 1770
Size: 612 320 56 91 691
Fragments arranged by size:
691 612 320 91 56
MscI TGG'CCA
Cuts at: 0 1390 1770
Size: 1390 380
MslI CAynn'nnrTG
Cuts at: 0 361 388 736 1770
Size: 361 27 348 1034
Fragments arranged by size:
1034 361 348 27
MspA1I CmG'CkG
Cuts at: 0 136 294 929 1556 1770
Size: 136 158 635 627 214
Fragments arranged by size:
635 627 214 158 136
PflMI CCAn_nnn'nTGG
Cuts at: 0 956 1770
Size: 956 814
PfoI T'CCnGG_A
Cuts at: 0 55 651 1770
Size: 55 596 1119
Fragments arranged by size:
1119 596 55
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 633 665 1033 1065 1770
Size: 87 32 514 32 368 32 705
Fragments arranged by size:
705 514 368 87 32 32 32
PstI C_TGCA'G
Cuts at: 0 871 1169 1226 1770
Size: 871 298 57 544
Fragments arranged by size:
871 544 298 57
PvuII CAG'CTG
Cuts at: 0 136 294 1556 1770
Size: 136 158 1262 214
Fragments arranged by size:
1262 214 158 136
SapI GCTCTTCn'nnn_
Cuts at: 0 1228 1770
Size: 1228 542
SbfI CC_TGCA'GG
Cuts at: 0 1169 1770
Size: 1169 601
SfcI C'TryA_G
Cuts at: 0 867 1165 1222 1770
Size: 867 298 57 548
Fragments arranged by size:
867 548 298 57
SmaI CCC'GGG
Cuts at: 0 92 1770
Size: 92 1678
SmlI C'TyrA_G
Cuts at: 0 128 321 1105 1770
Size: 128 193 784 665
Fragments arranged by size:
784 665 193 128
SnaBI TAC'GTA
Cuts at: 0 447 1770
Size: 447 1323
StuI AGG'CCT
Cuts at: 0 1765 1770
Size: 1765 5
StyI C'CwwG_G
Cuts at: 0 236 1770
Size: 236 1534
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 1722 1770
Size: 556 1166 48
Fragments arranged by size:
1166 556 48
TatI w'GTAC_w
Cuts at: 0 19 1770
Size: 19 1751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 1770
Size: 55 32 1683
Fragments arranged by size:
1683 55 32
XhoI C'TCGA_G
Cuts at: 0 128 1770
Size: 128 1642
XmaI C'CCGG_G
Cuts at: 0 90 1770
Size: 90 1680
ZraI GAC'GTC
Cuts at: 0 1326 1770
Size: 1326 444
Enzymes that do cut:
AarI AatII Acc65I AcuI AflII AhdI AloI AvaI
BanI BanII BbsI BcgI BciVI BglI BplI BpmI
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI
EarI EciI Eco57MI EcoNI Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I PflMI PfoI PpiI PstI
PvuII SapI SbfI SfcI SmaI SmlI SnaBI StuI
StyI TaqII TatI TstI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflIII AgeI AleI AlfI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaWI BsgI
BsiEI BsiWI BsmI BspEI BsrGI BstAPI BstBI BstXI
BstZ17I CspCI DraI DraIII DrdI EagI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XmnI