Restriction map for pFN21AB5561
 (Circular) (Six-base) MAPSORT of: pFN21AB5561.seq  Check: 3844  from: 1  to: 6196



pFN21AB5561  6196 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4006     278 
   Size:         53      83     186     936    2470    2468

  Fragments arranged by size: 

                2470    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3352    3457    3539    1937 
   Size:       1415     105      82    4594

  Fragments arranged by size: 

                4594    1415     105      82
 
Acc65I G'GTAC_C

Cuts at:   3517    3517 
   Size:       6196
 
AclI AA'CG_TT

Cuts at:   1563    4323    4696    1563 
   Size:       2760     373    3063

  Fragments arranged by size: 

                3063    2760     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2432    3036    3102    4259    5643    5911    1632 
   Size:        800     604      66    1157    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1157     800     604     268      66
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5999
 
AflIII A'CryG_T

Cuts at:   1203    2037    3410    5095    6072    1203 
   Size:        834    1373    1685     977    1327

  Fragments arranged by size: 

                1685    1373    1327     977     834
 
AgeI A'CCGG_T

Cuts at:   5027    5027 
   Size:       6196
 
AhdI GACnn_n'nnGTC

Cuts at:   4925    4925 
   Size:       6196
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6196
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6196
 
AlwNI CAG_nnn'CTG

Cuts at:   3939    5511    3939 
   Size:       1572    4624
 
ApaI G_GGCC'C

Cuts at:   2995    2995 
   Size:       6196
 
ApaLI G'TGCA_C

Cuts at:   2148    4253    5409    2148 
   Size:       2105    1156    2935

  Fragments arranged by size: 

                2935    2105    1156
 
ApoI r'AATT_y

Cuts at:   1493    2533    2765    2910    3505    3731    3883    5992    1493 
   Size:       1040     232     145     595     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1040     595     232     226     152     145
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4750     160 
   Size:        872    3718    1606

  Fragments arranged by size: 

                3718    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6196
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3521    1870 
   Size:         73    1578    4545

  Fragments arranged by size: 

                4545    1578      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2370    2403    2370 
   Size:         33    6163
 
BamHI G'GATC_C

Cuts at:   3526    3893    3526 
   Size:        367    5829
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3517    4972     618 
   Size:        325     217     627    1730    1455    1842

  Fragments arranged by size: 

                1842    1730    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2995    3515    1391 
   Size:       1604     520    4072

  Fragments arranged by size: 

                4072    1604     520
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3204     928 
   Size:        971    1305    3920

  Fragments arranged by size: 

                3920    1305     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4387    4421    1903 
   Size:         34    2450      34    3678

  Fragments arranged by size: 

                3678    2450      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4088    5298    4088 
   Size:       1210    4986
 
BclI T'GATC_A

Cuts at:   1548    2422    1548 
   Size:        874    5322
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4807     136 
   Size:        107     122      71     631     605    3135    1525

  Fragments arranged by size: 

                3135    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6191    6191 
   Size:       6196
 
BlpI GC'TnA_GC

Cuts at:   3613    3613 
   Size:       6196
 
Bme1580I G_kGCm'C

Cuts at:   1790    2152    2995    4257    5413    1790 
   Size:        362     843    1262    1156    2573

  Fragments arranged by size: 

                2573    1262    1156     843     362
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6196
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3443    3927    4885 
   Size:        416      53      83     399      24    2026     484     958

Cuts at:   4885     442 
   Size:       1753

  Fragments arranged by size: 

                2026    1753     958     484     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6196
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4856    1239 
   Size:       3617    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2917    2917 
   Size:       6196
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4321    5186    5484    4321 
   Size:        865     298    5033

  Fragments arranged by size: 

                5033     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2090    4859     882 
   Size:        617     591    2769    2219

  Fragments arranged by size: 

                2769    2219     617     591
 
BsaAI yAC'GTr

Cuts at:    493    1204    3987     493 
   Size:        711    2783    2702

  Fragments arranged by size: 

                2783    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6190    6190 
   Size:       6196
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2675    4003    4385    5020 
   Size:         53      83     186     936    1142    1328     382     635

Cuts at:   5020     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1328    1142     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2686    4629    5027    5301    5448    5859    2686 
   Size:       1943     398     274     147     411    3023

  Fragments arranged by size: 

                3023    1943     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5971

  Fragments arranged by size: 

                5971     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2026    1186 
   Size:        840    5356
 
BseYI C'CCAG_C

Cuts at:   5399    5895    5399 
   Size:        496    5700
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5623
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3646    4407    5435    1540 
   Size:        275     186    1645     761    1028    2301

  Fragments arranged by size: 

                2301    1645    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2152    2310    4257    4342    5413    2152 
   Size:        158    1947      85    1071    2935

  Fragments arranged by size: 

                2935    1947    1071     158      85
 
BsmI GAATG_Cn'

Cuts at:   3707    3800    3707 
   Size:         93    6103
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2152    2310    2995    3515    4257    4342    5413 
   Size:        399     362     158     685     520     742      85    1071

Cuts at:   5413    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     685     520     399     362     158
                  85
 
BspEI T'CCGG_A

Cuts at:   2686    5859    2686 
   Size:       3173    3023
 
BspHI T'CATG_A

Cuts at:   4085    5815    4085 
   Size:       1730    4466
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3551     844 
   Size:       2707    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4083    1394 
   Size:        429    2260    3507

  Fragments arranged by size: 

                3507    2260     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2117    2953    2974    4691    4865      65 
   Size:       1183     869     836      21    1717     174    1396

  Fragments arranged by size: 

                1717    1396    1183     869     836     174      21
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4840    5027    1700 
   Size:        110    3030     187    2869

  Fragments arranged by size: 

                3030    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2437      96 
   Size:       2341    3855
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6196
 
BssSI C'ACGA_G

Cuts at:   4256    5268    4256 
   Size:       1012    5184
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2943     794 
   Size:       2149    4047
 
BstBI TT'CG_AA

Cuts at:   5000    5000 
   Size:       6196
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6196
 
BstYI r'GATC_y

Cuts at:   1972    2689    2902    3526    3893    4278    4295    5747    5833 
   Size:        717     213     624     367     385      17    1452      86

Cuts at:   5833    5845    6191    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     717     624     385     367     346     213
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    3376     513 
   Size:        552     939    1372    3333

  Fragments arranged by size: 

                3333    1372     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5457

  Fragments arranged by size: 

                5457     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2665    3712    4506    4526    2665 
   Size:       1047     794      20    4335

  Fragments arranged by size: 

                4335    1047     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6196
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6161
 
DraI TTT'AAA

Cuts at:   3500    3500 
   Size:       6196
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5203     809 
   Size:        507    3887    1802

  Fragments arranged by size: 

                3887    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2194    3643    4532 
   Size:         54    1425      50     275      18     364    1449     889

Cuts at:   4532    6161       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1449    1425     889     364     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3643    1537 
   Size:        275    1831    4090

  Fragments arranged by size: 

                4090    1831     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2448    3078    3285    4126    1353 
   Size:       1095     630     207     841    3423

  Fragments arranged by size: 

                3423    1095     841     630     207
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4783    5157    5303     387 
   Size:       1064     138    3194     374     146    1280

  Fragments arranged by size: 

                3194    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2432    3036    3102    4259    4856    5643    5911 
   Size:        393     800     604      66    1157     597     787     268

Cuts at:   5911    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1157     800     787     604     597     393     268
                  66
 
EcoO109I rG'GnC_Cy

Cuts at:   2431    2992    2431 
   Size:        561    5635
 
EcoRI G'AATT_C

Cuts at:   3505    3883    3505 
   Size:        378    5818
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2198    2198 
   Size:       6196
 
FspI TGC'GCA

Cuts at:   4702    4702 
   Size:       6196
 
HaeII r_GCGC'y

Cuts at:   1678    5343    1678 
   Size:       3665    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2531    2563    2808    2840    4837 
   Size:         32     135      32     959      32     245      32    1997

Cuts at:   4837    4869    4911    4943    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1997     959     245     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3353    3540    3786     669 
   Size:        988     281    1415     187     246    3079

  Fragments arranged by size: 

                3079    1415     988     281     246     187
 
HindIII A'AGCT_T

Cuts at:    748    2290    3174     748 
   Size:       1542     884    3770

  Fragments arranged by size: 

                3770    1542     884
 
HpaI GTT'AAC

Cuts at:   3786    3786 
   Size:       6196
 
KpnI G_GTAC'C

Cuts at:   3521    3521 
   Size:       6196
 
MfeI C'AATT_G

Cuts at:   3795    3795 
   Size:       6196
 
MluI A'CGCG_T

Cuts at:   6072    6072 
   Size:       6196
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2709    5310    5494    1305 
   Size:       1404    2601     184    2007

  Fragments arranged by size: 

                2601    2007    1404     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2196      10 
   Size:         54    1425     343     364    4010

  Fragments arranged by size: 

                4010    1425     364     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2718    4154    4513    4672     518 
   Size:        648    1552    1436     359     159    2042

  Fragments arranged by size: 

                2042    1552    1436     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3612    4289    5437    5682    1606 
   Size:       2006     677    1148     245    2120

  Fragments arranged by size: 

                2120    2006    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    3376     513 
   Size:        552     939    1372    3333

  Fragments arranged by size: 

                3333    1372     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6196
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6196
 
NotI GC'GGCC_GC

Cuts at:   3643    3643 
   Size:       6196
 
NspI r_CATG'y

Cuts at:   1132    1229    2041    3414    3554    5099    1132 
   Size:         97     812    1373     140    1545    2229

  Fragments arranged by size: 

                2229    1545    1373     812     140      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6196
 
PciI A'CATG_T

Cuts at:   2037    3410    5095    2037 
   Size:       1373    1685    3138

  Fragments arranged by size: 

                3138    1685    1373
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6196
 
PmeI GTTT'AAAC

Cuts at:   3500    3500 
   Size:       6196
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4265    4297    5778    5810    4265 
   Size:         32    1481      32    4651

  Fragments arranged by size: 

                4651    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2431    2431 
   Size:       6196
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6196
 
PsiI TTA'TAA

Cuts at:   3766    3766 
   Size:       6196
 
PspOMI G'GGCC_C

Cuts at:   2991    2991 
   Size:       6196
 
PstI C_TGCA'G

Cuts at:    830    3548     830 
   Size:       2718    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6196
 
SalI G'TCGA_C

Cuts at:   1936    3351    3538    1936 
   Size:       1415     187    4594

  Fragments arranged by size: 

                4594    1415     187
 
SbfI CC_TGCA'GG

Cuts at:   3548    3548 
   Size:       6196
 
ScaI AGT'ACT

Cuts at:   4444    6156    4444 
   Size:       1712    4484
 
SfcI C'TryA_G

Cuts at:    826    1045    2332    2667    3117    3544    4679    5360    5551 
   Size:        219    1287     335     450     427    1135     681     191

Cuts at:   5551     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1287    1135     681     450     427     335     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6196
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6196
 
SmaI CCC'GGG

Cuts at:   1872    3523    1872 
   Size:       1651    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4300    5201    5463     820 
   Size:        197     926    2357     901     262    1553

  Fragments arranged by size: 

                2357    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3987     493 
   Size:       3494    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6196
 
SphI G_CATG'C

Cuts at:   1132    3554    1132 
   Size:       2422    3774
 
SspI AAT'ATT

Cuts at:      5      52    4120       5 
   Size:         47    4068    2081

  Fragments arranged by size: 

                4068    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6196
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3376    3635     513 
   Size:        552     939    1372     259    3074

  Fragments arranged by size: 

                3074    1372     939     552     259
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4223    4240    4393    4578    5023     325 
   Size:       1255    2643      17     153     185     445    1498

  Fragments arranged by size: 

                2643    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2437    4442    6154 
   Size:        274      80      33      51    1443     460    2005    1712

Cuts at:   6154      96 
   Size:        138

  Fragments arranged by size: 

                2005    1712    1443     460     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6156    6188    6156 
   Size:         32    6164
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6196
 
XbaI T'CTAG_A

Cuts at:   2483    2501    3532    2483 
   Size:         18    1031    5147

  Fragments arranged by size: 

                5147    1031      18
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6196
 
XmaI C'CCGG_G

Cuts at:   1870    3521    1870 
   Size:       1651    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4325    4325 
   Size:       6196
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4004     276 
   Size:         53      83     186     936    2470    2468

  Fragments arranged by size: 

                2470    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BbeI    BbvCI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com