(Circular) (Six-base) MAPSORT of: pFN21AB5561.seq Check: 3844 from: 1 to: 6196 pFN21AB5561 6196 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3352 3457 3539 1937 Size: 1415 105 82 4594 Fragments arranged by size: 4594 1415 105 82 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 3036 3102 4259 5643 5911 1632 Size: 800 604 66 1157 1384 268 1917 Fragments arranged by size: 1917 1384 1157 800 604 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 2037 3410 5095 6072 1203 Size: 834 1373 1685 977 1327 Fragments arranged by size: 1685 1373 1327 977 834 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 4925 4925 Size: 6196 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6196 AlwNI CAG_nnn'CTG Cuts at: 3939 5511 3939 Size: 1572 4624 ApaI G_GGCC'C Cuts at: 2995 2995 Size: 6196 ApaLI G'TGCA_C Cuts at: 2148 4253 5409 2148 Size: 2105 1156 2935 Fragments arranged by size: 2935 2105 1156 ApoI r'AATT_y Cuts at: 1493 2533 2765 2910 3505 3731 3883 5992 1493 Size: 1040 232 145 595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 595 232 226 152 145 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 3521 1870 Size: 73 1578 4545 Fragments arranged by size: 4545 1578 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2370 2403 2370 Size: 33 6163 BamHI G'GATC_C Cuts at: 3526 3893 3526 Size: 367 5829 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3517 4972 618 Size: 325 217 627 1730 1455 1842 Fragments arranged by size: 1842 1730 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2995 3515 1391 Size: 1604 520 4072 Fragments arranged by size: 4072 1604 520 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3204 928 Size: 971 1305 3920 Fragments arranged by size: 3920 1305 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 2422 1548 Size: 874 5322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6191 6191 Size: 6196 BlpI GC'TnA_GC Cuts at: 3613 3613 Size: 6196 Bme1580I G_kGCm'C Cuts at: 1790 2152 2995 4257 5413 1790 Size: 362 843 1262 1156 2573 Fragments arranged by size: 2573 1262 1156 843 362 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3443 3927 4885 Size: 416 53 83 399 24 2026 484 958 Cuts at: 4885 442 Size: 1753 Fragments arranged by size: 2026 1753 958 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4856 1239 Size: 3617 2579 Bpu10I CC'TnA_GC Cuts at: 2917 2917 Size: 6196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4321 5186 5484 4321 Size: 865 298 5033 Fragments arranged by size: 5033 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2090 4859 882 Size: 617 591 2769 2219 Fragments arranged by size: 2769 2219 617 591 BsaAI yAC'GTr Cuts at: 493 1204 3987 493 Size: 711 2783 2702 Fragments arranged by size: 2783 2702 711 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2675 4003 4385 5020 Size: 53 83 186 936 1142 1328 382 635 Cuts at: 5020 275 Size: 1451 Fragments arranged by size: 1451 1328 1142 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2686 4629 5027 5301 5448 5859 2686 Size: 1943 398 274 147 411 3023 Fragments arranged by size: 3023 1943 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5971 Fragments arranged by size: 5971 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 1186 Size: 840 5356 BseYI C'CCAG_C Cuts at: 5399 5895 5399 Size: 496 5700 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5623 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 2310 4257 4342 5413 2152 Size: 158 1947 85 1071 2935 Fragments arranged by size: 2935 1947 1071 158 85 BsmI GAATG_Cn' Cuts at: 3707 3800 3707 Size: 93 6103 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2310 2995 3515 4257 4342 5413 Size: 399 362 158 685 520 742 85 1071 Cuts at: 5413 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 685 520 399 362 158 85 BspEI T'CCGG_A Cuts at: 2686 5859 2686 Size: 3173 3023 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3551 844 Size: 2707 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4083 1394 Size: 429 2260 3507 Fragments arranged by size: 3507 2260 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2117 2953 2974 4691 4865 65 Size: 1183 869 836 21 1717 174 1396 Fragments arranged by size: 1717 1396 1183 869 836 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 4840 5027 1700 Size: 110 3030 187 2869 Fragments arranged by size: 3030 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2437 96 Size: 2341 3855 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 2943 794 Size: 2149 4047 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstYI r'GATC_y Cuts at: 1972 2689 2902 3526 3893 4278 4295 5747 5833 Size: 717 213 624 367 385 17 1452 86 Cuts at: 5833 5845 6191 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 717 624 385 367 346 213 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3376 513 Size: 552 939 1372 3333 Fragments arranged by size: 3333 1372 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 2665 3712 4506 4526 2665 Size: 1047 794 20 4335 Fragments arranged by size: 4335 1047 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6161 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 3643 4532 Size: 54 1425 50 275 18 364 1449 889 Cuts at: 4532 6161 8 Size: 1629 43 Fragments arranged by size: 1629 1449 1425 889 364 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2448 3078 3285 4126 1353 Size: 1095 630 207 841 3423 Fragments arranged by size: 3423 1095 841 630 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 3036 3102 4259 4856 5643 5911 Size: 393 800 604 66 1157 597 787 268 Cuts at: 5911 1239 Size: 1524 Fragments arranged by size: 1524 1157 800 787 604 597 393 268 66 EcoO109I rG'GnC_Cy Cuts at: 2431 2992 2431 Size: 561 5635 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2198 2198 Size: 6196 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 5343 1678 Size: 3665 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2531 2563 2808 2840 4837 Size: 32 135 32 959 32 245 32 1997 Cuts at: 4837 4869 4911 4943 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1997 959 245 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3353 3540 3786 669 Size: 988 281 1415 187 246 3079 Fragments arranged by size: 3079 1415 988 281 246 187 HindIII A'AGCT_T Cuts at: 748 2290 3174 748 Size: 1542 884 3770 Fragments arranged by size: 3770 1542 884 HpaI GTT'AAC Cuts at: 3786 3786 Size: 6196 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2709 5310 5494 1305 Size: 1404 2601 184 2007 Fragments arranged by size: 2601 2007 1404 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2196 10 Size: 54 1425 343 364 4010 Fragments arranged by size: 4010 1425 364 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2718 4154 4513 4672 518 Size: 648 1552 1436 359 159 2042 Fragments arranged by size: 2042 1552 1436 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3612 4289 5437 5682 1606 Size: 2006 677 1148 245 2120 Fragments arranged by size: 2120 2006 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3376 513 Size: 552 939 1372 3333 Fragments arranged by size: 3333 1372 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 2041 3414 3554 5099 1132 Size: 97 812 1373 140 1545 2229 Fragments arranged by size: 2229 1545 1373 812 140 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6196 PciI A'CATG_T Cuts at: 2037 3410 5095 2037 Size: 1373 1685 3138 Fragments arranged by size: 3138 1685 1373 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2431 Size: 6196 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6196 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PspOMI G'GGCC_C Cuts at: 2991 2991 Size: 6196 PstI C_TGCA'G Cuts at: 830 3548 830 Size: 2718 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3351 3538 1936 Size: 1415 187 4594 Fragments arranged by size: 4594 1415 187 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 4444 6156 4444 Size: 1712 4484 SfcI C'TryA_G Cuts at: 826 1045 2332 2667 3117 3544 4679 5360 5551 Size: 219 1287 335 450 427 1135 681 191 Cuts at: 5551 826 Size: 1471 Fragments arranged by size: 1471 1287 1135 681 450 427 335 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4300 5201 5463 820 Size: 197 926 2357 901 262 1553 Fragments arranged by size: 2357 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 2004 3376 3635 513 Size: 552 939 1372 259 3074 Fragments arranged by size: 3074 1372 939 552 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4223 4240 4393 4578 5023 325 Size: 1255 2643 17 153 185 445 1498 Fragments arranged by size: 2643 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2437 4442 6154 Size: 274 80 33 51 1443 460 2005 1712 Cuts at: 6154 96 Size: 138 Fragments arranged by size: 2005 1712 1443 460 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6156 6188 6156 Size: 32 6164 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 2483 2501 3532 2483 Size: 18 1031 5147 Fragments arranged by size: 5147 1031 18 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 4325 4325 Size: 6196 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI