(Circular) (Six-base) MAPSORT of: pFN21AB3608.seq Check: 5383 from: 1 to: 5335 pFN21AB3608 5335 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3145 278 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2678 1937 Size: 741 4594 Acc65I G'GTAC_C Cuts at: 2656 2656 Size: 5335 AclI AA'CG_TT Cuts at: 1563 3462 3835 1563 Size: 1899 373 3063 Fragments arranged by size: 3063 1899 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 3398 4782 5050 1632 Size: 1004 762 1384 268 1917 Fragments arranged by size: 1917 1384 1004 762 268 AfeI AGC'GCT Cuts at: 2297 2297 Size: 5335 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5138 AflIII A'CryG_T Cuts at: 1203 4234 5211 1203 Size: 3031 977 1327 Fragments arranged by size: 3031 1327 977 AgeI A'CCGG_T Cuts at: 4166 4166 Size: 5335 AhdI GACnn_n'nnGTC Cuts at: 4064 4064 Size: 5335 AleI CACnn'nnGTG Cuts at: 1166 2216 2345 1166 Size: 1050 129 4156 Fragments arranged by size: 4156 1050 129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5335 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2146 2178 2146 Size: 32 5303 AlwNI CAG_nnn'CTG Cuts at: 3078 4650 3078 Size: 1572 3763 ApaLI G'TGCA_C Cuts at: 3392 4548 3392 Size: 1156 4179 ApoI r'AATT_y Cuts at: 1493 2585 2644 2870 3022 5131 1493 Size: 1092 59 226 152 2109 1697 Fragments arranged by size: 2109 1697 1092 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 3889 160 Size: 872 2857 1606 Fragments arranged by size: 2857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 AvaI C'yCGr_G Cuts at: 1870 1943 2488 2529 2660 1870 Size: 73 545 41 131 4545 Fragments arranged by size: 4545 545 131 73 41 BamHI G'GATC_C Cuts at: 2665 3032 2665 Size: 367 4968 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2235 2656 4111 618 Size: 325 217 627 448 421 1455 1842 Fragments arranged by size: 1842 1455 627 448 421 325 217 BanII G_rGCy'C Cuts at: 1391 2654 1391 Size: 1263 4072 BbeI G_GCGC'C Cuts at: 2239 2239 Size: 5335 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2436 928 Size: 971 537 3827 Fragments arranged by size: 3827 971 537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3526 3560 1903 Size: 34 50 34 1505 34 3678 Fragments arranged by size: 3678 1505 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3227 4437 3227 Size: 1210 4125 BclI T'GATC_A Cuts at: 1548 1548 Size: 5335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3946 136 Size: 107 122 71 631 605 2274 1525 Fragments arranged by size: 2274 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2226 5330 2226 Size: 3104 2231 BlpI GC'TnA_GC Cuts at: 2178 2752 2178 Size: 574 4761 Bme1580I G_kGCm'C Cuts at: 1790 3396 4552 1790 Size: 1606 1156 2573 Fragments arranged by size: 2573 1606 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3066 4024 442 Size: 416 53 83 399 24 1649 958 1753 Fragments arranged by size: 1753 1649 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5335 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2157 2157 Size: 5335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2139 2150 3995 1239 Size: 900 11 1845 2579 Fragments arranged by size: 2579 1845 900 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3460 4325 4623 3460 Size: 865 298 4172 Fragments arranged by size: 4172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2055 2615 3998 882 Size: 617 556 560 1383 2219 Fragments arranged by size: 2219 1383 617 560 556 BsaAI yAC'GTr Cuts at: 493 1204 3126 493 Size: 711 1922 2702 Fragments arranged by size: 2702 1922 711 BsaBI GATnn'nnATC Cuts at: 5329 5329 Size: 5335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2236 3142 3524 4159 Size: 53 83 186 936 703 906 382 635 Cuts at: 4159 275 Size: 1451 Fragments arranged by size: 1451 936 906 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2580 3768 4166 4440 4587 4998 2580 Size: 1188 398 274 147 411 2917 Fragments arranged by size: 2917 1188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 1178 Size: 30 165 30 743 30 4337 Fragments arranged by size: 4337 743 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5335 BseYI C'CCAG_C Cuts at: 4538 5034 4538 Size: 496 4839 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 2312 2348 2369 2451 1163 Size: 573 303 273 36 21 82 4047 Fragments arranged by size: 4047 573 303 273 82 36 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2785 3546 4574 1540 Size: 275 186 784 761 1028 2301 Fragments arranged by size: 2301 1028 784 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3396 3481 4552 3396 Size: 85 1071 4179 Fragments arranged by size: 4179 1071 85 BsmI GAATG_Cn' Cuts at: 2846 2939 2846 Size: 93 5242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2654 3396 3481 4552 1391 Size: 399 864 742 85 1071 2174 Fragments arranged by size: 2174 1071 864 742 399 85 BspEI T'CCGG_A Cuts at: 4998 4998 Size: 5335 BspHI T'CATG_A Cuts at: 3224 4954 3224 Size: 1730 3605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2690 844 Size: 1846 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2028 2109 3222 1394 Size: 429 205 81 1113 3507 Fragments arranged by size: 3507 1113 429 205 81 BsrDI GCAATG_nn' Cuts at: 65 1248 2172 3830 4004 65 Size: 1183 924 1658 174 1396 Fragments arranged by size: 1658 1396 1183 924 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2182 2559 3979 4166 1700 Size: 110 372 377 1420 187 2869 Fragments arranged by size: 2869 1420 377 372 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5335 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5335 BssSI C'ACGA_G Cuts at: 2142 3395 4407 2142 Size: 1253 1012 3070 Fragments arranged by size: 3070 1253 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5335 BstBI TT'CG_AA Cuts at: 4139 4139 Size: 5335 BstEII G'GTnAC_C Cuts at: 1184 2533 1184 Size: 1349 3986 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2266 Size: 5335 BstYI r'GATC_y Cuts at: 1972 2226 2665 3032 3417 3434 4886 4972 4984 Size: 254 439 367 385 17 1452 86 12 Cuts at: 4984 5330 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 439 385 367 346 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2415 513 Size: 552 1350 3433 Fragments arranged by size: 3433 1350 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4596 Fragments arranged by size: 4596 683 56 BtsI GCAGTG_nn' Cuts at: 2336 2851 3645 3665 2336 Size: 515 794 20 4006 Fragments arranged by size: 4006 794 515 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5300 DraI TTT'AAA Cuts at: 2639 2639 Size: 5335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4342 809 Size: 507 3026 1802 Fragments arranged by size: 3026 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2273 2782 Size: 54 1425 50 275 18 354 89 509 Cuts at: 2782 3671 5300 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 509 354 275 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2782 1537 Size: 275 970 4090 Fragments arranged by size: 4090 970 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3265 1353 Size: 1912 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2546 3922 4296 4442 387 Size: 1064 138 957 1376 374 146 1280 Fragments arranged by size: 1376 1280 1064 957 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2139 2150 2636 3398 3995 4782 5050 Size: 393 507 11 486 762 597 787 268 Cuts at: 5050 1239 Size: 1524 Fragments arranged by size: 1524 787 762 597 507 486 393 268 11 EcoRI G'AATT_C Cuts at: 2644 3022 2644 Size: 378 4957 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2419 2419 Size: 5335 FspI TGC'GCA Cuts at: 3841 3841 Size: 5335 HaeII r_GCGC'y Cuts at: 1678 2239 2299 4482 1678 Size: 561 60 2183 2531 Fragments arranged by size: 2531 2183 561 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3976 4008 4050 4082 1373 Size: 32 135 32 2404 32 42 32 2626 Fragments arranged by size: 2626 2404 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2679 2925 669 Size: 988 281 741 246 3079 Fragments arranged by size: 3079 988 741 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5335 HpaI GTT'AAC Cuts at: 2925 2925 Size: 5335 KasI G'GCGC_C Cuts at: 2235 2235 Size: 5335 KpnI G_GTAC'C Cuts at: 2660 2660 Size: 5335 MfeI C'AATT_G Cuts at: 2934 2934 Size: 5335 MluI A'CGCG_T Cuts at: 5211 5211 Size: 5335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2068 2070 4449 4633 1305 Size: 763 2 2379 184 2007 Fragments arranged by size: 2379 2007 763 184 2 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3513 Fragments arranged by size: 3513 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2345 2446 2598 3293 3652 3811 Size: 648 1050 129 101 152 695 359 159 Cuts at: 3811 518 Size: 2042 Fragments arranged by size: 2042 1050 695 648 359 159 152 129 101 MspA1I CmG'CkG Cuts at: 1606 2353 2465 2751 3428 4576 4821 1606 Size: 747 112 286 677 1148 245 2120 Fragments arranged by size: 2120 1148 747 677 286 245 112 NaeI GCC'GGC Cuts at: 2184 2561 2184 Size: 377 4958 NarI GG'CG_CC Cuts at: 2236 2236 Size: 5335 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4783 NdeI CA'TA_TG Cuts at: 387 387 Size: 5335 NgoMIV G'CCGG_C Cuts at: 2182 2559 2182 Size: 377 4958 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5335 NotI GC'GGCC_GC Cuts at: 2782 2782 Size: 5335 NspI r_CATG'y Cuts at: 1132 1229 2445 2693 4238 1132 Size: 97 1216 248 1545 2229 Fragments arranged by size: 2229 1545 1216 248 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5335 PciI A'CATG_T Cuts at: 4234 4234 Size: 5335 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5335 PmeI GTTT'AAAC Cuts at: 2639 2639 Size: 5335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2146 2178 3404 3436 4917 4949 2146 Size: 32 1226 32 1481 32 2532 Fragments arranged by size: 2532 1481 1226 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5335 PsiI TTA'TAA Cuts at: 2905 2905 Size: 5335 PstI C_TGCA'G Cuts at: 830 2687 830 Size: 1857 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5335 PvuII CAG'CTG Cuts at: 2353 2465 2353 Size: 112 5223 SalI G'TCGA_C Cuts at: 1936 2677 1936 Size: 741 4594 SbfI CC_TGCA'GG Cuts at: 2687 2687 Size: 5335 ScaI AGT'ACT Cuts at: 3583 5295 3583 Size: 1712 3623 SfcI C'TryA_G Cuts at: 826 1045 2683 3818 4499 4690 826 Size: 219 1638 1135 681 191 1471 Fragments arranged by size: 1638 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2237 2237 Size: 5335 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5335 SmaI CCC'GGG Cuts at: 1872 2490 2662 1872 Size: 618 172 4545 Fragments arranged by size: 4545 618 172 SmlI C'TyrA_G Cuts at: 820 1017 1943 3439 4340 4602 820 Size: 197 926 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3126 493 Size: 2633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5335 SphI G_CATG'C Cuts at: 1132 2445 2693 1132 Size: 1313 248 3774 Fragments arranged by size: 3774 1313 248 SspI AAT'ATT Cuts at: 5 52 3259 5 Size: 47 3207 2081 Fragments arranged by size: 3207 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5335 StyI C'CwwG_G Cuts at: 513 1065 2568 2626 2774 513 Size: 552 1503 58 148 3074 Fragments arranged by size: 3074 1503 552 148 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 3362 3379 3532 3717 4162 325 Size: 1255 777 1005 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1005 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3581 5293 96 Size: 274 80 33 51 1443 1604 1712 138 Fragments arranged by size: 1712 1604 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5295 5327 5295 Size: 32 5303 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5335 XbaI T'CTAG_A Cuts at: 2671 2671 Size: 5335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2422 2422 Size: 5335 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5335 XmaI C'CCGG_G Cuts at: 1870 2488 2660 1870 Size: 618 172 4545 Fragments arranged by size: 4545 618 172 XmnI GAAnn'nnTTC Cuts at: 3464 3464 Size: 5335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3143 276 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI