(Circular) (Six-base) MAPSORT of: pFN21AE0107.seq Check: 5525 from: 1 to: 5554 pFN21AE0107 5554 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2388 3364 278 Size: 53 83 186 936 852 976 2468 Fragments arranged by size: 2468 976 936 852 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 1937 Size: 960 4594 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2544 3617 5001 5269 1632 Size: 437 475 1073 1384 268 1917 Fragments arranged by size: 1917 1384 1073 475 437 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 2321 2579 4453 5430 1203 Size: 1118 258 1874 977 1327 Fragments arranged by size: 1874 1327 1118 977 258 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 2429 1166 Size: 1263 4291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5554 AlwNI CAG_nnn'CTG Cuts at: 2356 2417 2847 3297 4869 2356 Size: 61 430 450 1572 3041 Fragments arranged by size: 3041 1572 450 430 61 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2863 3089 3241 5350 1493 Size: 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2742 2879 1870 Size: 73 799 137 4545 Fragments arranged by size: 4545 799 137 73 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2875 4330 618 Size: 325 217 627 1088 1455 1842 Fragments arranged by size: 1842 1455 1088 627 325 217 BanII G_rGCy'C Cuts at: 1391 2246 2873 1391 Size: 855 627 4072 Fragments arranged by size: 4072 855 627 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 928 Size: 971 272 4311 Fragments arranged by size: 4311 971 272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2789 2823 3745 3779 1903 Size: 34 852 34 922 34 3678 Fragments arranged by size: 3678 922 852 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2690 3446 4656 2690 Size: 756 1210 3588 Fragments arranged by size: 3588 1210 756 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2073 4165 136 Size: 107 122 71 631 605 401 2092 1525 Fragments arranged by size: 2092 1525 631 605 401 122 107 71 BglII A'GATC_T Cuts at: 5549 5549 Size: 5554 BlpI GC'TnA_GC Cuts at: 2971 2971 Size: 5554 Bme1580I G_kGCm'C Cuts at: 1790 2329 3615 4771 1790 Size: 539 1286 1156 2573 Fragments arranged by size: 2573 1286 1156 539 BmgBI CAC'GTC Cuts at: 1318 2338 1318 Size: 1020 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2535 3285 4243 Size: 416 53 83 399 24 1118 750 958 Cuts at: 4243 442 Size: 1753 Fragments arranged by size: 1753 1118 958 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 4214 1239 Size: 863 2112 2579 Fragments arranged by size: 2579 2112 863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 3679 4544 4842 2054 Size: 1625 865 298 2766 Fragments arranged by size: 2766 1625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2752 4217 882 Size: 617 1253 1465 2219 Fragments arranged by size: 2219 1465 1253 617 BsaAI yAC'GTr Cuts at: 493 1204 2580 3345 493 Size: 711 1376 765 2702 Fragments arranged by size: 2702 1376 765 711 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2385 3361 3743 Size: 53 83 186 936 509 343 976 382 Cuts at: 3743 4378 275 Size: 635 1451 Fragments arranged by size: 1451 976 936 635 509 382 343 186 83 53 BsaWI w'CCGG_w Cuts at: 2529 3987 4385 4659 4806 5217 2529 Size: 1458 398 274 147 411 2866 Fragments arranged by size: 2866 1458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2459 2489 1178 Size: 30 165 30 1056 30 4243 Fragments arranged by size: 4243 1056 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2102 2245 1186 Size: 913 3 143 4495 Fragments arranged by size: 4495 913 143 3 BseYI C'CCAG_C Cuts at: 4757 5253 4757 Size: 496 5058 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2006 2087 2102 2510 1163 Size: 573 270 81 15 408 4207 Fragments arranged by size: 4207 573 408 270 81 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3004 3765 4793 1540 Size: 275 186 1003 761 1028 2301 Fragments arranged by size: 2301 1028 1003 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2246 3615 3700 4771 2246 Size: 1369 85 1071 3029 Fragments arranged by size: 3029 1369 1071 85 BsmI GAATG_Cn' Cuts at: 3065 3158 3065 Size: 93 5461 BsmBI CGTCTCn'nnnn_ Cuts at: 2027 2027 Size: 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2246 2329 2873 3615 3700 4771 1391 Size: 399 456 83 544 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 544 456 399 85 83 BspEI T'CCGG_A Cuts at: 5217 5217 Size: 5554 BspHI T'CATG_A Cuts at: 2160 3443 5173 2160 Size: 1283 1730 2541 Fragments arranged by size: 2541 1730 1283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2604 2909 844 Size: 1760 305 3489 Fragments arranged by size: 3489 1760 305 BsrBI CCG'CTC Cuts at: 1394 1823 2134 2310 2346 3441 1394 Size: 429 311 176 36 1095 3507 Fragments arranged by size: 3507 1095 429 311 176 36 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 4223 65 Size: 1183 2801 174 1396 Fragments arranged by size: 2801 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4198 4385 1700 Size: 110 2388 187 2869 Fragments arranged by size: 2869 2388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5554 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5554 BssSI C'ACGA_G Cuts at: 2024 3614 4626 2024 Size: 1590 1012 2952 Fragments arranged by size: 2952 1590 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2356 794 Size: 1562 3992 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstXI CCAn_nnnn'nTGG Cuts at: 2375 2375 Size: 5554 BstYI r'GATC_y Cuts at: 1972 2295 2884 3251 3636 3653 5105 5191 5203 Size: 323 589 367 385 17 1452 86 12 Cuts at: 5203 5549 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 589 385 367 346 323 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2188 2368 2457 513 Size: 552 939 184 180 89 3610 Fragments arranged by size: 3610 939 552 184 180 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2328 487 Size: 683 56 1102 3713 Fragments arranged by size: 3713 1102 683 56 BtsI GCAGTG_nn' Cuts at: 3070 3864 3884 3070 Size: 794 20 4740 Fragments arranged by size: 4740 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DraIII CAC_nnn'GTG Cuts at: 2110 2125 2110 Size: 15 5539 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2041 4561 809 Size: 507 725 2520 1802 Fragments arranged by size: 2520 1802 725 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 3001 3890 Size: 54 1425 50 275 18 649 522 889 Cuts at: 3890 5519 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 649 522 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 1537 Size: 275 1189 4090 Fragments arranged by size: 4090 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3484 1353 Size: 692 1439 3423 Fragments arranged by size: 3423 1439 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2144 4141 4515 4661 387 Size: 1064 138 555 1997 374 146 1280 Fragments arranged by size: 1997 1280 1064 555 374 146 138 EcoICRI GAG'CTC Cuts at: 2244 2244 Size: 5554 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2102 2544 3617 4214 5001 5269 Size: 393 437 33 442 1073 597 787 268 Cuts at: 5269 1239 Size: 1524 Fragments arranged by size: 1524 1073 787 597 442 437 393 268 33 EcoO109I rG'GnC_Cy Cuts at: 2143 2175 2739 2143 Size: 32 564 4958 Fragments arranged by size: 4958 564 32 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 FspI TGC'GCA Cuts at: 4060 4060 Size: 5554 HaeII r_GCGC'y Cuts at: 1678 4701 1678 Size: 3023 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 4195 4227 4269 Size: 32 135 32 887 32 1704 32 42 Cuts at: 4269 4301 1373 Size: 32 2626 Fragments arranged by size: 2626 1704 887 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4668 4852 1305 Size: 3363 184 2007 Fragments arranged by size: 3363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3732 Fragments arranged by size: 3732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2373 2429 3512 3871 4030 518 Size: 648 1207 56 1083 359 159 2042 Fragments arranged by size: 2042 1207 1083 648 359 159 56 MspA1I CmG'CkG Cuts at: 1606 2353 2970 3647 4795 5040 1606 Size: 747 617 677 1148 245 2120 Fragments arranged by size: 2120 1148 747 677 617 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2457 513 Size: 552 939 453 3610 Fragments arranged by size: 3610 939 552 453 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2325 2473 2912 4457 1132 Size: 97 1096 148 439 1545 2229 Fragments arranged by size: 2229 1545 1096 439 148 97 PasI CC'CwG_GG Cuts at: 1348 2146 1348 Size: 798 4756 PciI A'CATG_T Cuts at: 2321 4453 2321 Size: 2132 3422 PflMI CCAn_nnn'nTGG Cuts at: 1108 2463 1108 Size: 1355 4199 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 5136 5168 3623 Size: 32 1481 32 4009 Fragments arranged by size: 4009 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2175 2739 2175 Size: 564 4990 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5554 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PstI C_TGCA'G Cuts at: 830 2361 2906 830 Size: 1531 545 3478 Fragments arranged by size: 3478 1531 545 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 PvuII CAG'CTG Cuts at: 2353 2353 Size: 5554 SacI G_AGCT'C Cuts at: 2246 2246 Size: 5554 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SanDI GG'GwC_CC Cuts at: 2739 2739 Size: 5554 SapI GCTCTTCn'nnn_ Cuts at: 2045 2045 Size: 5554 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 3802 5514 3802 Size: 1712 3842 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 5554 SfcI C'TryA_G Cuts at: 826 1045 2357 2902 4037 4718 4909 826 Size: 219 1312 545 1135 681 191 1471 Fragments arranged by size: 1471 1312 1135 681 545 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2744 2881 1872 Size: 872 137 4545 Fragments arranged by size: 4545 872 137 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 3658 4559 4821 820 Size: 197 926 126 1589 901 262 1553 Fragments arranged by size: 1589 1553 926 901 262 197 126 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 2495 1517 Size: 978 4576 StyI C'CwwG_G Cuts at: 513 1065 2004 2457 2993 513 Size: 552 939 453 536 3074 Fragments arranged by size: 3074 939 552 536 453 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3581 3598 3751 3936 4381 325 Size: 1255 2001 17 153 185 445 1498 Fragments arranged by size: 2001 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3800 5512 96 Size: 274 80 33 51 1443 1823 1712 138 Fragments arranged by size: 1823 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5514 5546 5514 Size: 32 5522 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5554 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2742 2879 1870 Size: 872 137 4545 Fragments arranged by size: 4545 872 137 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2386 3362 276 Size: 53 83 186 936 852 976 2468 Fragments arranged by size: 2468 976 936 852 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI