Restriction map for pFN21AE0107
 (Circular) (Six-base) MAPSORT of: pFN21AE0107.seq  Check: 5525  from: 1  to: 5554



pFN21AE0107  5554 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:44  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2388    3364     278 
   Size:         53      83     186     936     852     976    2468

  Fragments arranged by size: 

                2468     976     936     852     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2897    1937 
   Size:        960    4594
 
Acc65I G'GTAC_C

Cuts at:   2875    2875 
   Size:       5554
 
AclI AA'CG_TT

Cuts at:   1563    3681    4054    1563 
   Size:       2118     373    3063

  Fragments arranged by size: 

                3063    2118     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2069    2544    3617    5001    5269    1632 
   Size:        437     475    1073    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1073     475     437     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5357
 
AflIII A'CryG_T

Cuts at:   1203    2321    2579    4453    5430    1203 
   Size:       1118     258    1874     977    1327

  Fragments arranged by size: 

                1874    1327    1118     977     258
 
AgeI A'CCGG_T

Cuts at:   4385    4385 
   Size:       5554
 
AhdI GACnn_n'nnGTC

Cuts at:   4283    4283 
   Size:       5554
 
AleI CACnn'nnGTG

Cuts at:   1166    2429    1166 
   Size:       1263    4291
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5554
 
AlwNI CAG_nnn'CTG

Cuts at:   2356    2417    2847    3297    4869    2356 
   Size:         61     430     450    1572    3041

  Fragments arranged by size: 

                3041    1572     450     430      61
 
ApaLI G'TGCA_C

Cuts at:   3611    4767    3611 
   Size:       1156    4398
 
ApoI r'AATT_y

Cuts at:   1493    2863    3089    3241    5350    1493 
   Size:       1370     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1370     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4108     160 
   Size:        872    3076    1606

  Fragments arranged by size: 

                3076    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5554
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2742    2879    1870 
   Size:         73     799     137    4545

  Fragments arranged by size: 

                4545     799     137      73
 
BamHI G'GATC_C

Cuts at:   2884    3251    2884 
   Size:        367    5187
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2875    4330     618 
   Size:        325     217     627    1088    1455    1842

  Fragments arranged by size: 

                1842    1455    1088     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2246    2873    1391 
   Size:        855     627    4072

  Fragments arranged by size: 

                4072     855     627
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2171     928 
   Size:        971     272    4311

  Fragments arranged by size: 

                4311     971     272
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2789    2823    3745    3779    1903 
   Size:         34     852      34     922      34    3678

  Fragments arranged by size: 

                3678     922     852      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2690    3446    4656    2690 
   Size:        756    1210    3588

  Fragments arranged by size: 

                3588    1210     756
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5554
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2073    4165     136 
   Size:        107     122      71     631     605     401    2092    1525

  Fragments arranged by size: 

                2092    1525     631     605     401     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5549    5549 
   Size:       5554
 
BlpI GC'TnA_GC

Cuts at:   2971    2971 
   Size:       5554
 
Bme1580I G_kGCm'C

Cuts at:   1790    2329    3615    4771    1790 
   Size:        539    1286    1156    2573

  Fragments arranged by size: 

                2573    1286    1156     539
 
BmgBI CAC'GTC

Cuts at:   1318    2338    1318 
   Size:       1020    4534
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2535    3285    4243 
   Size:        416      53      83     399      24    1118     750     958

Cuts at:   4243     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1118     958     750     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5554
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2102    4214    1239 
   Size:        863    2112    2579

  Fragments arranged by size: 

                2579    2112     863
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2054    3679    4544    4842    2054 
   Size:       1625     865     298    2766

  Fragments arranged by size: 

                2766    1625     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2752    4217     882 
   Size:        617    1253    1465    2219

  Fragments arranged by size: 

                2219    1465    1253     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2580    3345     493 
   Size:        711    1376     765    2702

  Fragments arranged by size: 

                2702    1376     765     711
 
BsaBI GATnn'nnATC

Cuts at:   5548    5548 
   Size:       5554
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2042    2385    3361    3743 
   Size:         53      83     186     936     509     343     976     382

Cuts at:   3743    4378     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     976     936     635     509     382     343     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   2529    3987    4385    4659    4806    5217    2529 
   Size:       1458     398     274     147     411    2866

  Fragments arranged by size: 

                2866    1458     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2459    2489    1178 
   Size:         30     165      30    1056      30    4243

  Fragments arranged by size: 

                4243    1056     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2099    2102    2245    1186 
   Size:        913       3     143    4495

  Fragments arranged by size: 

                4495     913     143       3
 
BseYI C'CCAG_C

Cuts at:   4757    5253    4757 
   Size:        496    5058
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2006    2087    2102    2510    1163 
   Size:        573     270      81      15     408    4207

  Fragments arranged by size: 

                4207     573     408     270      81      15
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3004    3765    4793    1540 
   Size:        275     186    1003     761    1028    2301

  Fragments arranged by size: 

                2301    1028    1003     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2246    3615    3700    4771    2246 
   Size:       1369      85    1071    3029

  Fragments arranged by size: 

                3029    1369    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3065    3158    3065 
   Size:         93    5461
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2027    2027 
   Size:       5554
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2246    2329    2873    3615    3700    4771    1391 
   Size:        399     456      83     544     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     544     456     399      85      83
            
 
BspEI T'CCGG_A

Cuts at:   5217    5217 
   Size:       5554
 
BspHI T'CATG_A

Cuts at:   2160    3443    5173    2160 
   Size:       1283    1730    2541

  Fragments arranged by size: 

                2541    1730    1283
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2604    2909     844 
   Size:       1760     305    3489

  Fragments arranged by size: 

                3489    1760     305
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2134    2310    2346    3441    1394 
   Size:        429     311     176      36    1095    3507

  Fragments arranged by size: 

                3507    1095     429     311     176      36
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4049    4223      65 
   Size:       1183    2801     174    1396

  Fragments arranged by size: 

                2801    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4198    4385    1700 
   Size:        110    2388     187    2869

  Fragments arranged by size: 

                2869    2388     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5554
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5554
 
BssSI C'ACGA_G

Cuts at:   2024    3614    4626    2024 
   Size:       1590    1012    2952

  Fragments arranged by size: 

                2952    1590    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2356     794 
   Size:       1562    3992
 
BstBI TT'CG_AA

Cuts at:   4358    4358 
   Size:       5554
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5554
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2375    2375 
   Size:       5554
 
BstYI r'GATC_y

Cuts at:   1972    2295    2884    3251    3636    3653    5105    5191    5203 
   Size:        323     589     367     385      17    1452      86      12

Cuts at:   5203    5549    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     589     385     367     346     323      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2188    2368    2457     513 
   Size:        552     939     184     180      89    3610

  Fragments arranged by size: 

                3610     939     552     184     180      89
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2328     487 
   Size:        683      56    1102    3713

  Fragments arranged by size: 

                3713    1102     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3070    3864    3884    3070 
   Size:        794      20    4740

  Fragments arranged by size: 

                4740     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5554
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5519
 
DraI TTT'AAA

Cuts at:   2858    2858 
   Size:       5554
 
DraIII CAC_nnn'GTG

Cuts at:   2110    2125    2110 
   Size:         15    5539
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2041    4561     809 
   Size:        507     725    2520    1802

  Fragments arranged by size: 

                2520    1802     725     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2479    3001    3890 
   Size:         54    1425      50     275      18     649     522     889

Cuts at:   3890    5519       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     649     522     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3001    1537 
   Size:        275    1189    4090

  Fragments arranged by size: 

                4090    1189     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2045    3484    1353 
   Size:        692    1439    3423

  Fragments arranged by size: 

                3423    1439     692
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2144    4141    4515    4661     387 
   Size:       1064     138     555    1997     374     146    1280

  Fragments arranged by size: 

                1997    1280    1064     555     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2244    2244 
   Size:       5554
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2069    2102    2544    3617    4214    5001    5269 
   Size:        393     437      33     442    1073     597     787     268

Cuts at:   5269    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1073     787     597     442     437     393     268
                  33
 
EcoO109I rG'GnC_Cy

Cuts at:   2143    2175    2739    2143 
   Size:         32     564    4958

  Fragments arranged by size: 

                4958     564      32
 
EcoRI G'AATT_C

Cuts at:   2863    3241    2863 
   Size:        378    5176
 
FspI TGC'GCA

Cuts at:   4060    4060 
   Size:       5554
 
HaeII r_GCGC'y

Cuts at:   1678    4701    1678 
   Size:       3023    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2459    2491    4195    4227    4269 
   Size:         32     135      32     887      32    1704      32      42

Cuts at:   4269    4301    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1704     887     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2898    3144     669 
   Size:        988     281     960     246    3079

  Fragments arranged by size: 

                3079     988     960     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5554
 
HpaI GTT'AAC

Cuts at:   3144    3144 
   Size:       5554
 
KpnI G_GTAC'C

Cuts at:   2879    2879 
   Size:       5554
 
MfeI C'AATT_G

Cuts at:   3153    3153 
   Size:       5554
 
MluI A'CGCG_T

Cuts at:   5430    5430 
   Size:       5554
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4668    4852    1305 
   Size:       3363     184    2007

  Fragments arranged by size: 

                3363    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3732

  Fragments arranged by size: 

                3732    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2373    2429    3512    3871    4030     518 
   Size:        648    1207      56    1083     359     159    2042

  Fragments arranged by size: 

                2042    1207    1083     648     359     159      56
 
MspA1I CmG'CkG

Cuts at:   1606    2353    2970    3647    4795    5040    1606 
   Size:        747     617     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     747     677     617     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2457     513 
   Size:        552     939     453    3610

  Fragments arranged by size: 

                3610     939     552     453
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5554
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5554
 
NotI GC'GGCC_GC

Cuts at:   3001    3001 
   Size:       5554
 
NspI r_CATG'y

Cuts at:   1132    1229    2325    2473    2912    4457    1132 
   Size:         97    1096     148     439    1545    2229

  Fragments arranged by size: 

                2229    1545    1096     439     148      97
 
PasI CC'CwG_GG

Cuts at:   1348    2146    1348 
   Size:        798    4756
 
PciI A'CATG_T

Cuts at:   2321    4453    2321 
   Size:       2132    3422
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2463    1108 
   Size:       1355    4199
 
PmeI GTTT'AAAC

Cuts at:   2858    2858 
   Size:       5554
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3623    3655    5136    5168    3623 
   Size:         32    1481      32    4009

  Fragments arranged by size: 

                4009    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2175    2739    2175 
   Size:        564    4990
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5554
 
PsiI TTA'TAA

Cuts at:   3124    3124 
   Size:       5554
 
PstI C_TGCA'G

Cuts at:    830    2361    2906     830 
   Size:       1531     545    3478

  Fragments arranged by size: 

                3478    1531     545
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5554
 
PvuII CAG'CTG

Cuts at:   2353    2353 
   Size:       5554
 
SacI G_AGCT'C

Cuts at:   2246    2246 
   Size:       5554
 
SalI G'TCGA_C

Cuts at:   1936    2896    1936 
   Size:        960    4594
 
SanDI GG'GwC_CC

Cuts at:   2739    2739 
   Size:       5554
 
SapI GCTCTTCn'nnn_

Cuts at:   2045    2045 
   Size:       5554
 
SbfI CC_TGCA'GG

Cuts at:   2906    2906 
   Size:       5554
 
ScaI AGT'ACT

Cuts at:   3802    5514    3802 
   Size:       1712    3842
 
SexAI A'CCwGG_T

Cuts at:   2121    2121 
   Size:       5554
 
SfcI C'TryA_G

Cuts at:    826    1045    2357    2902    4037    4718    4909     826 
   Size:        219    1312     545    1135     681     191    1471

  Fragments arranged by size: 

                1471    1312    1135     681     545     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5554
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5554
 
SmaI CCC'GGG

Cuts at:   1872    2744    2881    1872 
   Size:        872     137    4545

  Fragments arranged by size: 

                4545     872     137
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2069    3658    4559    4821     820 
   Size:        197     926     126    1589     901     262    1553

  Fragments arranged by size: 

                1589    1553     926     901     262     197     126
 
SnaBI TAC'GTA

Cuts at:    493    3345     493 
   Size:       2852    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5554
 
SphI G_CATG'C

Cuts at:   1132    2912    1132 
   Size:       1780    3774
 
SspI AAT'ATT

Cuts at:      5      52    3478       5 
   Size:         47    3426    2081

  Fragments arranged by size: 

                3426    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2495    1517 
   Size:        978    4576
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2457    2993     513 
   Size:        552     939     453     536    3074

  Fragments arranged by size: 

                3074     939     552     536     453
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3581    3598    3751    3936    4381     325 
   Size:       1255    2001      17     153     185     445    1498

  Fragments arranged by size: 

                2001    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3800    5512      96 
   Size:        274      80      33      51    1443    1823    1712     138

  Fragments arranged by size: 

                1823    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5514    5546    5514 
   Size:         32    5522
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5554
 
XbaI T'CTAG_A

Cuts at:   2890    2890 
   Size:       5554
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5554
 
XmaI C'CCGG_G

Cuts at:   1870    2742    2879    1870 
   Size:        872     137    4545

  Fragments arranged by size: 

                4545     872     137
 
XmnI GAAnn'nnTTC

Cuts at:   3683    3683 
   Size:       5554
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2386    3362     276 
   Size:         53      83     186     936     852     976    2468

  Fragments arranged by size: 

                2468     976     936     852     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI    PvuII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI  BstZ17I   Bsu36I    EcoNI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI
    RsrII    SacII     SfiI     SfoI     SrfI     SwaI     XcmI
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