(Circular) (Six-base) MAPSORT of: pFN21AE0075.seq Check: 7576 from: 1 to: 9046 pFN21AE0075 9046 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3315 6856 278 Size: 53 83 186 936 1779 3541 2468 Fragments arranged by size: 3541 2468 1779 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 6389 1937 Size: 637 3815 4594 Fragments arranged by size: 4594 3815 637 Acc65I G'GTAC_C Cuts at: 6367 6367 Size: 9046 AclI AA'CG_TT Cuts at: 1563 6345 7173 7546 1563 Size: 4782 828 373 3063 Fragments arranged by size: 4782 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3553 3647 4531 4544 4553 6006 7109 8493 Size: 1921 94 884 13 9 1453 1103 1384 Cuts at: 8493 8761 1632 Size: 268 1917 Fragments arranged by size: 1921 1917 1453 1384 1103 884 268 94 13 9 AfeI AGC'GCT Cuts at: 2526 4756 2526 Size: 2230 6816 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8849 AflIII A'CryG_T Cuts at: 1203 2577 4023 4410 5367 7945 8922 1203 Size: 1374 1446 387 957 2578 977 1327 Fragments arranged by size: 2578 1446 1374 1327 977 957 387 AgeI A'CCGG_T Cuts at: 7877 7877 Size: 9046 AhdI GACnn_n'nnGTC Cuts at: 5875 7775 5875 Size: 1900 7146 AleI CACnn'nnGTG Cuts at: 1166 4022 4313 6113 1166 Size: 2856 291 1800 4099 Fragments arranged by size: 4099 2856 1800 291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5366 5388 6101 1184 Size: 4182 22 713 4129 Fragments arranged by size: 4182 4129 713 22 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6047 6079 6047 Size: 32 9014 AlwNI CAG_nnn'CTG Cuts at: 2061 2169 3449 4554 4590 4723 6789 8361 2061 Size: 108 1280 1105 36 133 2066 1572 2746 Fragments arranged by size: 2746 2066 1572 1280 1105 133 108 36 ApaI G_GGCC'C Cuts at: 6016 6026 6037 6016 Size: 10 11 9025 Fragments arranged by size: 9025 11 10 ApaLI G'TGCA_C Cuts at: 6146 7103 8259 6146 Size: 957 1156 6933 Fragments arranged by size: 6933 1156 957 ApoI r'AATT_y Cuts at: 1493 2792 2938 3480 5976 6355 6581 6733 8842 Size: 1299 146 542 2496 379 226 152 2109 Cuts at: 8842 1493 Size: 1697 Fragments arranged by size: 2496 2109 1697 1299 542 379 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 4746 7600 160 Size: 872 3714 2854 1606 Fragments arranged by size: 3714 2854 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9046 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2366 4172 4311 4350 5848 6183 Size: 73 131 292 1806 139 39 1498 335 Cuts at: 6183 6327 6371 1870 Size: 144 44 4545 Fragments arranged by size: 4545 1806 1498 335 292 144 139 131 73 44 39 AvrII C'CTAG_G Cuts at: 2749 2749 Size: 9046 BamHI G'GATC_C Cuts at: 6376 6743 6376 Size: 367 8679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 3404 4304 5390 6367 Size: 325 217 627 282 1335 900 1086 977 Cuts at: 6367 7822 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1335 1086 977 900 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 2034 4109 4179 4908 6016 6026 6037 6365 Size: 643 2075 70 729 1108 10 11 328 Cuts at: 6365 1391 Size: 4072 Fragments arranged by size: 4072 2075 1108 729 643 328 70 11 10 BbeI G_GCGC'C Cuts at: 5394 5394 Size: 9046 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5131 5785 928 Size: 971 3232 654 4189 Fragments arranged by size: 4189 3232 971 654 BbvCI CC'TCA_GC Cuts at: 2859 4094 5705 2859 Size: 1235 1611 6200 Fragments arranged by size: 6200 1611 1235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5107 5141 7237 7271 1903 Size: 34 3170 34 2096 34 3678 Fragments arranged by size: 3678 3170 2096 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6938 8148 6938 Size: 1210 7836 BclI T'GATC_A Cuts at: 1548 1548 Size: 9046 BfrBI ATG'CAT Cuts at: 4577 4577 Size: 9046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2236 3647 4302 Size: 107 122 71 631 605 564 1411 655 Cuts at: 4302 4817 7657 136 Size: 515 2840 1525 Fragments arranged by size: 2840 1525 1411 655 631 605 564 515 122 107 71 BglII A'GATC_T Cuts at: 4056 9041 4056 Size: 4985 4061 BlpI GC'TnA_GC Cuts at: 4041 6463 4041 Size: 2422 6624 Bme1580I G_kGCm'C Cuts at: 1790 2074 5250 6016 6026 6037 6090 6150 7107 Size: 284 3176 766 10 11 53 60 957 Cuts at: 7107 8263 1790 Size: 1156 2573 Fragments arranged by size: 3176 2573 1156 957 766 284 60 53 11 10 BmgBI CAC'GTC Cuts at: 1318 3657 6256 6307 1318 Size: 2339 2599 51 4057 Fragments arranged by size: 4057 2599 2339 51 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 3467 4138 Size: 416 53 83 399 24 865 1185 671 Cuts at: 4138 4600 4915 6777 7735 442 Size: 462 315 1862 958 1753 Fragments arranged by size: 1862 1753 1185 958 865 671 462 416 399 315 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9046 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3166 4066 6209 3166 Size: 900 2143 6003 Fragments arranged by size: 6003 2143 900 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 2210 2537 3212 3713 4141 5381 5544 Size: 870 101 327 675 501 428 1240 163 Cuts at: 5544 7706 1239 Size: 2162 2579 Fragments arranged by size: 2579 2162 1240 870 675 501 428 327 163 101 Bpu10I CC'TnA_GC Cuts at: 2859 4094 5598 5705 2859 Size: 1235 1504 107 6200 Fragments arranged by size: 6200 1504 1235 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7171 8036 8334 7171 Size: 865 298 7883 Fragments arranged by size: 7883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2363 7709 882 Size: 617 864 5346 2219 Fragments arranged by size: 5346 2219 864 617 BsaAI yAC'GTr Cuts at: 493 1204 4024 6244 6837 493 Size: 711 2820 2220 593 2702 Fragments arranged by size: 2820 2702 2220 711 593 BsaBI GATnn'nnATC Cuts at: 3804 9040 3804 Size: 5236 3810 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 3312 3423 5391 Size: 53 83 186 936 642 1137 111 1968 Cuts at: 5391 6853 7235 7870 275 Size: 1462 382 635 1451 Fragments arranged by size: 1968 1462 1451 1137 936 642 635 382 186 111 83 53 BsaWI w'CCGG_w Cuts at: 6258 7479 7877 8151 8298 8709 6258 Size: 1221 398 274 147 411 6595 Fragments arranged by size: 6595 1221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2394 2402 2424 3630 Size: 30 165 30 969 22 8 22 1206 Cuts at: 3630 3660 4124 4154 4468 4498 5354 5384 1178 Size: 30 464 30 314 30 856 30 4840 Fragments arranged by size: 4840 1206 969 856 464 314 165 30 30 30 30 30 30 22 22 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 2396 3035 5726 6283 6332 1186 Size: 1126 84 639 2691 557 49 3900 Fragments arranged by size: 3900 2691 1126 639 557 84 49 BseYI C'CCAG_C Cuts at: 2162 2835 3381 4285 4751 5407 5609 5733 6018 Size: 673 546 904 466 656 202 124 285 Cuts at: 6018 6058 8249 8745 2162 Size: 40 2191 496 2463 Fragments arranged by size: 2463 2191 904 673 656 546 496 466 285 202 124 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 4085 4365 4523 5101 1163 Size: 573 402 1947 280 158 578 5108 Fragments arranged by size: 5108 1947 578 573 402 280 158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2084 2904 5163 5199 6496 7257 Size: 275 186 83 820 2259 36 1297 761 Cuts at: 7257 8285 1540 Size: 1028 2301 Fragments arranged by size: 2301 2259 1297 1028 820 761 275 186 83 36 BsiHKAI G_wGCw'C Cuts at: 3129 3470 4118 5367 6150 7107 7192 8263 3129 Size: 341 648 1249 783 957 85 1071 3912 Fragments arranged by size: 3912 1249 1071 957 783 648 341 85 BsmI GAATG_Cn' Cuts at: 5992 6317 6557 6650 5992 Size: 325 240 93 8388 Fragments arranged by size: 8388 325 240 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4314 5958 4314 Size: 1644 7402 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2074 3129 3470 4109 4118 4179 Size: 399 244 40 1055 341 639 9 61 Cuts at: 4179 4908 5250 5367 6016 6026 6037 6090 6150 Size: 729 342 117 649 10 11 53 60 Cuts at: 6150 6365 7107 7192 8263 1391 Size: 215 742 85 1071 2174 Fragments arranged by size: 2174 1071 1055 742 729 649 639 399 342 341 244 215 117 85 61 60 53 40 11 10 9 BspEI T'CCGG_A Cuts at: 8709 8709 Size: 9046 BspHI T'CATG_A Cuts at: 2475 6935 8665 2475 Size: 4460 1730 2856 Fragments arranged by size: 4460 2856 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4143 6401 844 Size: 3299 2258 3489 Fragments arranged by size: 3489 3299 2258 BsrBI CCG'CTC Cuts at: 1394 1823 3710 6933 1394 Size: 429 1887 3223 3507 Fragments arranged by size: 3507 3223 1887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3673 7541 7715 65 Size: 1183 2425 3868 174 1396 Fragments arranged by size: 3868 2425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 2157 4673 7690 7877 1700 Size: 110 273 74 2516 3017 187 2869 Fragments arranged by size: 3017 2869 2516 273 187 110 74 BsrGI T'GTAC_A Cuts at: 96 2580 96 Size: 2484 6562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9046 BssSI C'ACGA_G Cuts at: 5126 7106 8118 5126 Size: 1980 1012 6054 Fragments arranged by size: 6054 1980 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9046 BstBI TT'CG_AA Cuts at: 6278 7850 6278 Size: 1572 7474 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9046 BstXI CCAn_nnnn'nTGG Cuts at: 2444 3283 4258 5407 6073 2444 Size: 839 975 1149 666 5417 Fragments arranged by size: 5417 1149 975 839 666 BstYI r'GATC_y Cuts at: 1972 4056 5429 6376 6743 7128 7145 8597 8683 Size: 2084 1373 947 367 385 17 1452 86 Cuts at: 8683 8695 9041 1972 Size: 12 346 1977 Fragments arranged by size: 2084 1977 1452 1373 947 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2575 2575 Size: 9046 Bsu36I CC'TnA_GG Cuts at: 3885 5550 3885 Size: 1665 7381 BtgI C'CryG_G Cuts at: 513 1065 2004 2041 3408 3762 5250 6072 6220 Size: 552 939 37 1367 354 1488 822 148 Cuts at: 6220 513 Size: 3339 Fragments arranged by size: 3339 1488 1367 939 822 552 354 148 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8307 Fragments arranged by size: 8307 683 56 BtsI GCAGTG_nn' Cuts at: 2597 4165 6066 6562 7356 7376 2597 Size: 1568 1901 496 794 20 4267 Fragments arranged by size: 4267 1901 1568 794 496 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9011 DraI TTT'AAA Cuts at: 2269 6350 2269 Size: 4081 4965 DraIII CAC_nnn'GTG Cuts at: 5764 5764 Size: 9046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2412 3317 4643 5447 5695 8053 809 Size: 507 1096 905 1326 804 248 2358 1802 Fragments arranged by size: 2358 1802 1326 1096 905 804 507 248 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2081 2218 2901 Size: 54 1425 50 275 18 251 137 683 Cuts at: 2901 3330 3687 4829 5160 5322 5638 5939 6493 Size: 429 357 1142 331 162 316 301 554 Cuts at: 6493 7382 9011 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1142 889 683 554 429 357 331 316 301 275 251 162 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2081 2901 5160 6493 1537 Size: 275 269 820 2259 1333 4090 Fragments arranged by size: 4090 2259 1333 820 275 269 EarI CTCTTCn'nnn_ Cuts at: 1353 2445 2796 5057 6976 1353 Size: 1092 351 2261 1919 3423 Fragments arranged by size: 3423 2261 1919 1092 351 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5066 6190 7633 8007 8153 387 Size: 1064 138 3477 1124 1443 374 146 1280 Fragments arranged by size: 3477 1443 1280 1124 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2210 2537 3212 3553 3647 3713 Size: 393 477 101 327 675 341 94 66 Cuts at: 3713 4141 4531 4544 4553 5381 5544 6006 7109 Size: 428 390 13 9 828 163 462 1103 Cuts at: 7109 7706 8493 8761 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1103 828 787 675 597 477 462 428 393 390 341 327 268 163 101 94 66 13 9 EcoNI CCTnn'n_nnAGG Cuts at: 3202 5708 6230 3202 Size: 2506 522 6018 Fragments arranged by size: 6018 2506 522 EcoO109I rG'GnC_Cy Cuts at: 2334 4132 5750 5877 5917 6012 6022 6023 6033 Size: 1798 1618 127 40 95 10 1 10 Cuts at: 6033 6235 6324 2334 Size: 202 89 5056 Fragments arranged by size: 5056 1798 1618 202 127 95 89 40 10 10 1 EcoRI G'AATT_C Cuts at: 2792 6355 6733 2792 Size: 3563 378 5105 Fragments arranged by size: 5105 3563 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3615 3615 Size: 9046 FspI TGC'GCA Cuts at: 7552 7552 Size: 9046 HaeII r_GCGC'y Cuts at: 1678 2231 2528 3075 4758 5189 5394 8193 1678 Size: 553 297 547 1683 431 205 2799 2531 Fragments arranged by size: 2799 2531 1683 553 547 431 297 205 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2616 2648 4124 4156 5861 Size: 32 135 32 1044 32 1476 32 1705 Cuts at: 5861 5893 7687 7719 7761 7793 1373 Size: 32 1794 32 42 32 2626 Fragments arranged by size: 2626 1794 1705 1476 1044 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4710 6390 6636 669 Size: 988 281 2772 1680 246 3079 Fragments arranged by size: 3079 2772 1680 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9046 HpaI GTT'AAC Cuts at: 6636 6636 Size: 9046 KasI G'GCGC_C Cuts at: 5390 5390 Size: 9046 KpnI G_GTAC'C Cuts at: 6371 6371 Size: 9046 MfeI C'AATT_G Cuts at: 4974 6645 4974 Size: 1671 7375 MluI A'CGCG_T Cuts at: 8922 8922 Size: 9046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2380 4176 4485 5691 6366 8160 8344 1305 Size: 1075 1796 309 1206 675 1794 184 2007 Fragments arranged by size: 2007 1796 1794 1206 1075 675 309 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3332 5941 10 Size: 54 1425 343 1500 2609 3115 Fragments arranged by size: 3115 2609 1500 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3231 3326 4022 4313 5102 5174 5282 Size: 648 2065 95 696 291 789 72 108 Cuts at: 5282 6071 6113 7004 7363 7522 518 Size: 789 42 891 359 159 2042 Fragments arranged by size: 2065 2042 891 789 789 696 648 359 291 159 108 95 72 42 MspA1I CmG'CkG Cuts at: 1606 2043 2058 2233 2835 3336 3737 4040 4098 Size: 437 15 175 602 501 401 303 58 Cuts at: 4098 5652 6018 6462 7139 8287 8532 1606 Size: 1554 366 444 677 1148 245 2120 Fragments arranged by size: 2120 1554 1148 677 602 501 444 437 401 366 303 245 175 58 15 NaeI GCC'GGC Cuts at: 2085 2085 Size: 9046 NarI GG'CG_CC Cuts at: 5391 5391 Size: 9046 NcoI C'CATG_G Cuts at: 513 1065 2004 3762 6072 513 Size: 552 939 1758 2310 3487 Fragments arranged by size: 3487 2310 1758 939 552 NdeI CA'TA_TG Cuts at: 387 3211 387 Size: 2824 6222 NgoMIV G'CCGG_C Cuts at: 2083 2083 Size: 9046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9046 NotI GC'GGCC_GC Cuts at: 6493 6493 Size: 9046 NsiI A_TGCA'T Cuts at: 4579 4579 Size: 9046 NspI r_CATG'y Cuts at: 1132 1229 2581 4414 4873 5371 6404 7949 1132 Size: 97 1352 1833 459 498 1033 1545 2229 Fragments arranged by size: 2229 1833 1545 1352 1033 498 459 97 PasI CC'CwG_GG Cuts at: 1348 3400 6164 1348 Size: 2052 2764 4230 Fragments arranged by size: 4230 2764 2052 PciI A'CATG_T Cuts at: 2577 4410 5367 7945 2577 Size: 1833 957 2578 3678 Fragments arranged by size: 3678 2578 1833 957 PflMI CCAn_nnn'nTGG Cuts at: 1108 4533 1108 Size: 3425 5621 PfoI T'CCnGG_A Cuts at: 2796 3189 5382 5887 2796 Size: 393 2193 505 5955 Fragments arranged by size: 5955 2193 505 393 PmeI GTTT'AAAC Cuts at: 6350 6350 Size: 9046 PmlI CAC'GTG Cuts at: 4024 6244 4024 Size: 2220 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7115 7147 8628 8660 7115 Size: 32 1481 32 7501 Fragments arranged by size: 7501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4132 5877 5917 6235 4132 Size: 1745 40 318 6943 Fragments arranged by size: 6943 1745 318 40 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9046 PsiI TTA'TAA Cuts at: 6616 6616 Size: 9046 PspOMI G'GGCC_C Cuts at: 6012 6022 6033 6012 Size: 10 11 9025 Fragments arranged by size: 9025 11 10 PspXI vC'TCGA_Gb Cuts at: 4172 4172 Size: 9046 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4907 4939 4907 Size: 32 9014 PstI C_TGCA'G Cuts at: 830 3087 3454 5000 6303 6398 830 Size: 2257 367 1546 1303 95 3478 Fragments arranged by size: 3478 2257 1546 1303 367 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9046 PvuII CAG'CTG Cuts at: 2835 4098 2835 Size: 1263 7783 RsrII CG'GwC_CG Cuts at: 2140 2140 Size: 9046 SacII CC_GC'GG Cuts at: 2044 2044 Size: 9046 SalI G'TCGA_C Cuts at: 1936 6388 1936 Size: 4452 4594 SanDI GG'GwC_CC Cuts at: 5877 5917 5877 Size: 40 9006 SbfI CC_TGCA'GG Cuts at: 6398 6398 Size: 9046 ScaI AGT'ACT Cuts at: 7294 9006 7294 Size: 1712 7334 SfcI C'TryA_G Cuts at: 826 1045 3083 3450 3919 3937 4996 5950 6299 Size: 219 2038 367 469 18 1059 954 349 Cuts at: 6299 6394 7529 8210 8401 826 Size: 95 1135 681 191 1471 Fragments arranged by size: 2038 1471 1135 1059 954 681 469 367 349 219 191 95 18 SfoI GGC'GCC Cuts at: 5392 5392 Size: 9046 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9046 SmaI CCC'GGG Cuts at: 1872 2076 6329 6373 1872 Size: 204 4253 44 4545 Fragments arranged by size: 4545 4253 204 44 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 4172 7150 8051 8313 820 Size: 197 926 423 1806 2978 901 262 1553 Fragments arranged by size: 2978 1806 1553 926 901 423 262 197 SnaBI TAC'GTA Cuts at: 493 6837 493 Size: 6344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9046 SphI G_CATG'C Cuts at: 1132 6404 1132 Size: 5272 3774 SspI AAT'ATT Cuts at: 5 52 6970 5 Size: 47 6918 2081 Fragments arranged by size: 6918 2081 47 StuI AGG'CCT Cuts at: 1517 3197 3890 5703 1517 Size: 1680 693 1813 4860 Fragments arranged by size: 4860 1813 1680 693 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 2749 3762 4251 6072 6485 Size: 552 939 198 547 1013 489 1821 413 Cuts at: 6485 513 Size: 3074 Fragments arranged by size: 3074 1821 1013 939 552 547 489 413 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2380 3023 4439 5850 7073 7090 7243 Size: 1255 800 643 1416 1411 1223 17 153 Cuts at: 7243 7428 7873 325 Size: 185 445 1498 Fragments arranged by size: 1498 1416 1411 1255 1223 800 643 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 4026 5064 Size: 274 80 33 51 1443 603 1446 1038 Cuts at: 5064 7292 9004 96 Size: 2228 1712 138 Fragments arranged by size: 2228 1712 1446 1443 1038 603 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 2594 2626 3630 3662 9006 9038 2372 Size: 32 190 32 1004 32 5344 32 2380 Fragments arranged by size: 5344 2380 1004 190 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4796 5695 1316 Size: 3480 899 4667 Fragments arranged by size: 4667 3480 899 XbaI T'CTAG_A Cuts at: 6382 6382 Size: 9046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3280 3280 Size: 9046 XhoI C'TCGA_G Cuts at: 1943 2366 4172 1943 Size: 423 1806 6817 Fragments arranged by size: 6817 1806 423 XmaI C'CCGG_G Cuts at: 1870 2074 6327 6371 1870 Size: 204 4253 44 4545 Fragments arranged by size: 4545 4253 204 44 XmnI GAAnn'nnTTC Cuts at: 3302 7175 3302 Size: 3873 5173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3313 6854 276 Size: 53 83 186 936 1779 3541 2468 Fragments arranged by size: 3541 2468 1779 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BsiWI EcoICRI EcoRV FseI FspAI NruI PacI SacI SapI SexAI SfiI SrfI SwaI