Restriction map for pFN21AE0075
 (Linear) (Six-base) MAPSORT of: KIEE0075.seq  Check: 702  from: 1  to: 4341



KIEE0075.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AatII G_ACGT'C

Cuts at:      0    1310    4341 
   Size:       1310    3031
 
AccI GT'mk_AC

Cuts at:      0     569    4341 
   Size:        569    3772
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1548    1642    2526    2539    2548    4001    4341 
   Size:       1548      94     884      13       9    1453     340

  Fragments arranged by size: 

                1548    1453     884     340      94      13       9
 
AfeI AGC'GCT

Cuts at:      0     521    2751    4341 
   Size:        521    2230    1590

  Fragments arranged by size: 

                2230    1590     521
 
AflIII A'CryG_T

Cuts at:      0     572    2018    2405    3362    4341 
   Size:        572    1446     387     957     979

  Fragments arranged by size: 

                1446     979     957     572     387
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3870    4341 
   Size:       3870     471
 
AleI CACnn'nnGTG

Cuts at:      0    2017    2308    4108    4341 
   Size:       2017     291    1800     233

  Fragments arranged by size: 

                2017    1800     291     233
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3361    3383    4096    4341 
   Size:       3361      22     713     245

  Fragments arranged by size: 

                3361     713     245      22
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4042    4074    4341 
   Size:       4042      32     267

  Fragments arranged by size: 

                4042     267      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      56     164    1444    2549    2585    2718    4341 
   Size:         56     108    1280    1105      36     133    1623

  Fragments arranged by size: 

                1623    1280    1105     133     108      56      36
 
ApaI G_GGCC'C

Cuts at:      0    4011    4021    4032    4341 
   Size:       4011      10      11     309

  Fragments arranged by size: 

                4011     309      11      10
 
ApaLI G'TGCA_C

Cuts at:      0    4141    4341 
   Size:       4141     200
 
ApoI r'AATT_y

Cuts at:      0     787     933    1475    3971    4341 
   Size:        787     146     542    2496     370

  Fragments arranged by size: 

                2496     787     542     370     146
 
AseI AT'TA_AT

Cuts at:      0    2741    4341 
   Size:       2741    1600
 
AvaI C'yCGr_G

Cuts at:      0      69     361    2167    2306    2345    3843    4178    4322 
   Size:         69     292    1806     139      39    1498     335     144

Cuts at:   4322    4341 
   Size:         19

  Fragments arranged by size: 

                1806    1498     335     292     144     139      69      39
                  19
 
AvrII C'CTAG_G

Cuts at:      0     744    4341 
   Size:        744    3597
 
BanI G'GyrC_C

Cuts at:      0      64    1399    2299    3385    4341 
   Size:         64    1335     900    1086     956

  Fragments arranged by size: 

                1335    1086     956     900      64
 
BanII G_rGCy'C

Cuts at:      0      29    2104    2174    2903    4011    4021    4032    4341 
   Size:         29    2075      70     729    1108      10      11     309

  Fragments arranged by size: 

                2075    1108     729     309      70      29      11      10
            
 
BbeI G_GCGC'C

Cuts at:      0    3389    4341 
   Size:       3389     952
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3126    3780    4341 
   Size:       3126     654     561

  Fragments arranged by size: 

                3126     654     561
 
BbvCI CC'TCA_GC

Cuts at:      0     854    2089    3700    4341 
   Size:        854    1235    1611     641

  Fragments arranged by size: 

                1611    1235     854     641
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3102    3136    4341 
   Size:       3102      34    1205

  Fragments arranged by size: 

                3102    1205      34
 
BfrBI ATG'CAT

Cuts at:      0    2572    4341 
   Size:       2572    1769
 
BglI GCCn_nnn'nGGC

Cuts at:      0     231    1642    2297    2812    4341 
   Size:        231    1411     655     515    1529

  Fragments arranged by size: 

                1529    1411     655     515     231
 
BglII A'GATC_T

Cuts at:      0    2051    4341 
   Size:       2051    2290
 
BlpI GC'TnA_GC

Cuts at:      0    2036    4341 
   Size:       2036    2305
 
Bme1580I G_kGCm'C

Cuts at:      0      69    3245    4011    4021    4032    4085    4145    4341 
   Size:         69    3176     766      10      11      53      60     196

  Fragments arranged by size: 

                3176     766     196      69      60      53      11      10
            
 
BmgBI CAC'GTC

Cuts at:      0    1652    4251    4302    4341 
   Size:       1652    2599      51      39

  Fragments arranged by size: 

                2599    1652      51      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     277    1462    2133    2595    2910    4341 
   Size:        277    1185     671     462     315    1431

  Fragments arranged by size: 

                1431    1185     671     462     315     277
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1161    2061    4204    4341 
   Size:       1161     900    2143     137

  Fragments arranged by size: 

                2143    1161     900     137
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     205     532    1207    1708    2136    3376    3539 
   Size:        104     101     327     675     501     428    1240     163

Cuts at:   3539    4341 
   Size:        802

  Fragments arranged by size: 

                1240     802     675     501     428     327     163     104
                 101
 
Bpu10I CC'TnA_GC

Cuts at:      0     854    2089    3593    3700    4341 
   Size:        854    1235    1504     107     641

  Fragments arranged by size: 

                1504    1235     854     641     107
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     358    4341 
   Size:        358    3983
 
BsaAI yAC'GTr

Cuts at:      0    2019    4239    4341 
   Size:       2019    2220     102

  Fragments arranged by size: 

                2220    2019     102
 
BsaBI GATnn'nnATC

Cuts at:      0    1799    4341 
   Size:       1799    2542
 
BsaHI Gr'CG_yC

Cuts at:      0     170    1307    1418    3386    4341 
   Size:        170    1137     111    1968     955

  Fragments arranged by size: 

                1968    1137     955     170     111
 
BsaWI w'CCGG_w

Cuts at:      0    4253    4341 
   Size:       4253      88
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     367     389     397     419    1625    1655    2119    2149 
   Size:        367      22       8      22    1206      30     464      30

Cuts at:   2149    2463    2493    3349    3379    4341 
   Size:        314      30     856      30     962

  Fragments arranged by size: 

                1206     962     856     464     367     314      30      30
                  30      30      22      22       8
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     391    1030    3721    4278    4327    4341 
   Size:        307      84     639    2691     557      49      14

  Fragments arranged by size: 

                2691     639     557     307      84      49      14
 
BseYI C'CCAG_C

Cuts at:      0     157     830    1376    2280    2746    3402    3604    3728 
   Size:        157     673     546     904     466     656     202     124

Cuts at:   3728    4013    4053    4341 
   Size:        285      40     288

  Fragments arranged by size: 

                 904     673     656     546     466     288     285     202
                 157     124      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    2080    2360    2518    3096    4341 
   Size:        133    1947     280     158     578    1245

  Fragments arranged by size: 

                1947    1245     578     280     158     133
 
BsiEI CG_ry'CG

Cuts at:      0      79     899    3158    3194    4341 
   Size:         79     820    2259      36    1147

  Fragments arranged by size: 

                2259    1147     820      79      36
 
BsiHKAI G_wGCw'C

Cuts at:      0    1124    1465    2113    3362    4145    4341 
   Size:       1124     341     648    1249     783     196

  Fragments arranged by size: 

                1249    1124     783     648     341     196
 
BsmI GAATG_Cn'

Cuts at:      0    3987    4312    4341 
   Size:       3987     325      29

  Fragments arranged by size: 

                3987     325      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2309    3953    4341 
   Size:       2309    1644     388

  Fragments arranged by size: 

                2309    1644     388
 
Bsp1286I G_dGCh'C

Cuts at:      0      29      69    1124    1465    2104    2113    2174    2903 
   Size:         29      40    1055     341     639       9      61     729

Cuts at:   2903    3245    3362    4011    4021    4032    4085    4145    4341 
   Size:        342     117     649      10      11      53      60     196

  Fragments arranged by size: 

                1055     729     649     639     342     341     196     117
                  61      60      53      40      29      11      10       9
            
 
BspHI T'CATG_A

Cuts at:      0     470    4341 
   Size:        470    3871
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2138    4341 
   Size:       2138    2203
 
BsrBI CCG'CTC

Cuts at:      0    1705    4341 
   Size:       1705    2636
 
BsrDI GCAATG_nn'

Cuts at:      0    1668    4341 
   Size:       1668    2673
 
BsrFI r'CCGG_y

Cuts at:      0      78     152    2668    4341 
   Size:         78      74    2516    1673

  Fragments arranged by size: 

                2516    1673      78      74
 
BsrGI T'GTAC_A

Cuts at:      0     575    4341 
   Size:        575    3766
 
BssSI C'ACGA_G

Cuts at:      0    3121    4341 
   Size:       3121    1220
 
BstBI TT'CG_AA

Cuts at:      0    4273    4341 
   Size:       4273      68
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     439    1278    2253    3402    4068    4341 
   Size:        439     839     975    1149     666     273

  Fragments arranged by size: 

                1149     975     839     666     439     273
 
BstYI r'GATC_y

Cuts at:      0    2051    3424    4341 
   Size:       2051    1373     917

  Fragments arranged by size: 

                2051    1373     917
 
BstZ17I GTA'TAC

Cuts at:      0     570    4341 
   Size:        570    3771
 
Bsu36I CC'TnA_GG

Cuts at:      0    1880    3545    4341 
   Size:       1880    1665     796

  Fragments arranged by size: 

                1880    1665     796
 
BtgI C'CryG_G

Cuts at:      0      36    1403    1757    3245    4067    4215    4341 
   Size:         36    1367     354    1488     822     148     126

  Fragments arranged by size: 

                1488    1367     822     354     148     126      36
 
BtsI GCAGTG_nn'

Cuts at:      0     592    2160    4061    4341 
   Size:        592    1568    1901     280

  Fragments arranged by size: 

                1901    1568     592     280
 
DraI TTT'AAA

Cuts at:      0     264    4341 
   Size:        264    4077
 
DraIII CAC_nnn'GTG

Cuts at:      0    3759    4341 
   Size:       3759     582
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     407    1312    2638    3442    3690    4341 
   Size:        407     905    1326     804     248     651

  Fragments arranged by size: 

                1326     905     804     651     407     248
 
EaeI y'GGCC_r

Cuts at:      0      76     213     896    1325    1682    2824    3155    3317 
   Size:         76     137     683     429     357    1142     331     162

Cuts at:   3317    3633    3934    4341 
   Size:        316     301     407

  Fragments arranged by size: 

                1142     683     429     407     357     331     316     301
                 162     137      76
 
EagI C'GGCC_G

Cuts at:      0      76     896    3155    4341 
   Size:         76     820    2259    1186

  Fragments arranged by size: 

                2259    1186     820      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     440     791    3052    4341 
   Size:        440     351    2261    1289

  Fragments arranged by size: 

                2261    1289     440     351
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3061    4185    4341 
   Size:       3061    1124     156

  Fragments arranged by size: 

                3061    1124     156
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     205     532    1207    1548    1642    1708    2136 
   Size:        104     101     327     675     341      94      66     428

Cuts at:   2136    2526    2539    2548    3376    3539    4001    4341 
   Size:        390      13       9     828     163     462     340

  Fragments arranged by size: 

                 828     675     462     428     390     341     340     327
                 163     104     101      94      66      13       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1197    3703    4225    4341 
   Size:       1197    2506     522     116

  Fragments arranged by size: 

                2506    1197     522     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0     329    2127    3745    3872    3912    4007    4017    4018 
   Size:        329    1798    1618     127      40      95      10       1

Cuts at:   4018    4028    4230    4319    4341 
   Size:         10     202      89      22

  Fragments arranged by size: 

                1798    1618     329     202     127      95      89      40
                  22      10      10       1
 
EcoRI G'AATT_C

Cuts at:      0     787    4341 
   Size:        787    3554
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1610    4341 
   Size:       1610    2731
 
HaeII r_GCGC'y

Cuts at:      0     226     523    1070    2753    3184    3389    4341 
   Size:        226     297     547    1683     431     205     952

  Fragments arranged by size: 

                1683     952     547     431     297     226     205
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     611     643    2119    2151    3856    3888    4341 
   Size:        611      32    1476      32    1705      32     453

  Fragments arranged by size: 

                1705    1476     611     453      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2705    4341 
   Size:       2705    1636
 
KasI G'GCGC_C

Cuts at:      0    3385    4341 
   Size:       3385     956
 
MfeI C'AATT_G

Cuts at:      0    2969    4341 
   Size:       2969    1372
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     375    2171    2480    3686    4341 
   Size:        375    1796     309    1206     655

  Fragments arranged by size: 

                1796    1206     655     375     309
 
MscI TGG'CCA

Cuts at:      0    1327    3936    4341 
   Size:       1327    2609     405

  Fragments arranged by size: 

                2609    1327     405
 
MslI CAynn'nnrTG

Cuts at:      0    1226    1321    2017    2308    3097    3169    3277    4066 
   Size:       1226      95     696     291     789      72     108     789

Cuts at:   4066    4108    4341 
   Size:         42     233

  Fragments arranged by size: 

                1226     789     789     696     291     233     108      95
                  72      42
 
MspA1I CmG'CkG

Cuts at:      0      38      53     228     830    1331    1732    2035    2093 
   Size:         38      15     175     602     501     401     303      58

Cuts at:   2093    3647    4013    4341 
   Size:       1554     366     328

  Fragments arranged by size: 

                1554     602     501     401     366     328     303     175
                  58      38      15
 
NaeI GCC'GGC

Cuts at:      0      80    4341 
   Size:         80    4261
 
NarI GG'CG_CC

Cuts at:      0    3386    4341 
   Size:       3386     955
 
NcoI C'CATG_G

Cuts at:      0    1757    4067    4341 
   Size:       1757    2310     274

  Fragments arranged by size: 

                2310    1757     274
 
NdeI CA'TA_TG

Cuts at:      0    1206    4341 
   Size:       1206    3135
 
NgoMIV G'CCGG_C

Cuts at:      0      78    4341 
   Size:         78    4263
 
NsiI A_TGCA'T

Cuts at:      0    2574    4341 
   Size:       2574    1767
 
NspI r_CATG'y

Cuts at:      0     576    2409    2868    3366    4341 
   Size:        576    1833     459     498     975

  Fragments arranged by size: 

                1833     975     576     498     459
 
PasI CC'CwG_GG

Cuts at:      0    1395    4159    4341 
   Size:       1395    2764     182

  Fragments arranged by size: 

                2764    1395     182
 
PciI A'CATG_T

Cuts at:      0     572    2405    3362    4341 
   Size:        572    1833     957     979

  Fragments arranged by size: 

                1833     979     957     572
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2528    4341 
   Size:       2528    1813
 
PfoI T'CCnGG_A

Cuts at:      0     791    1184    3377    3882    4341 
   Size:        791     393    2193     505     459

  Fragments arranged by size: 

                2193     791     505     459     393
 
PmlI CAC'GTG

Cuts at:      0    2019    4239    4341 
   Size:       2019    2220     102

  Fragments arranged by size: 

                2220    2019     102
 
PpuMI rG'GwC_Cy

Cuts at:      0    2127    3872    3912    4230    4341 
   Size:       2127    1745      40     318     111

  Fragments arranged by size: 

                2127    1745     318     111      40
 
PspOMI G'GGCC_C

Cuts at:      0    4007    4017    4028    4341 
   Size:       4007      10      11     313

  Fragments arranged by size: 

                4007     313      11      10
 
PspXI vC'TCGA_Gb

Cuts at:      0    2167    4341 
   Size:       2167    2174
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2902    2934    4341 
   Size:       2902      32    1407

  Fragments arranged by size: 

                2902    1407      32
 
PstI C_TGCA'G

Cuts at:      0    1082    1449    2995    4298    4341 
   Size:       1082     367    1546    1303      43

  Fragments arranged by size: 

                1546    1303    1082     367      43
 
PvuII CAG'CTG

Cuts at:      0     830    2093    4341 
   Size:        830    1263    2248

  Fragments arranged by size: 

                2248    1263     830
 
RsrII CG'GwC_CG

Cuts at:      0     135    4341 
   Size:        135    4206
 
SacII CC_GC'GG

Cuts at:      0      39    4341 
   Size:         39    4302
 
SanDI GG'GwC_CC

Cuts at:      0    3872    3912    4341 
   Size:       3872      40     429

  Fragments arranged by size: 

                3872     429      40
 
SfcI C'TryA_G

Cuts at:      0    1078    1445    1914    1932    2991    3945    4294    4341 
   Size:       1078     367     469      18    1059     954     349      47

  Fragments arranged by size: 

                1078    1059     954     469     367     349      47      18
            
 
SfoI GGC'GCC

Cuts at:      0    3387    4341 
   Size:       3387     954
 
SmaI CCC'GGG

Cuts at:      0      71    4324    4341 
   Size:         71    4253      17

  Fragments arranged by size: 

                4253      71      17
 
SmlI C'TyrA_G

Cuts at:      0     361    2167    4341 
   Size:        361    1806    2174

  Fragments arranged by size: 

                2174    1806     361
 
StuI AGG'CCT

Cuts at:      0    1192    1885    3698    4341 
   Size:       1192     693    1813     643

  Fragments arranged by size: 

                1813    1192     693     643
 
StyI C'CwwG_G

Cuts at:      0     197     744    1757    2246    4067    4341 
   Size:        197     547    1013     489    1821     274

  Fragments arranged by size: 

                1821    1013     547     489     274     197
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     375    1018    2434    3845    4341 
   Size:        375     643    1416    1411     496

  Fragments arranged by size: 

                1416    1411     643     496     375
 
TatI w'GTAC_w

Cuts at:      0     575    2021    3059    4341 
   Size:        575    1446    1038    1282

  Fragments arranged by size: 

                1446    1282    1038     575
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     367     399     589     621    1625    1657    4341 
   Size:        367      32     190      32    1004      32    2684

  Fragments arranged by size: 

                2684    1004     367     190      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2791    3690    4341 
   Size:       2791     899     651

  Fragments arranged by size: 

                2791     899     651
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1275    4341 
   Size:       1275    3066
 
XhoI C'TCGA_G

Cuts at:      0     361    2167    4341 
   Size:        361    1806    2174

  Fragments arranged by size: 

                2174    1806     361
 
XmaI C'CCGG_G

Cuts at:      0      69    4322    4341 
   Size:         69    4253      19

  Fragments arranged by size: 

                4253      69      19
 
XmnI GAAnn'nnTTC

Cuts at:      0    1297    4341 
   Size:       1297    3044
 
ZraI GAC'GTC

Cuts at:      0    1308    4341 
   Size:       1308    3033
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    AvrII
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII     KasI     MfeI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI     PstI    PvuII    RsrII    SacII    SanDI     SfcI     SfoI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII     AgeI     AscI    AsiSI     BaeI
    BamHI    BciVI     BclI     BmtI    BpuEI    BsiWI    BspEI   BssHII
   BstAPI   BstEII    BtgZI     ClaI    CspCI  EcoICRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MluI     NheI     NotI
     NruI     PacI     PmeI     PpiI    PshAI     PsiI     PvuI     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI
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