(Circular) (Six-base) MAPSORT of: pFN21AE0073.seq Check: 1043 from: 1 to: 8665 pFN21AE0073 8665 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6475 278 Size: 53 83 186 936 4939 2468 Fragments arranged by size: 4939 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2688 6008 1937 Size: 751 3320 4594 Fragments arranged by size: 4594 3320 751 Acc65I G'GTAC_C Cuts at: 5986 5986 Size: 8665 AclI AA'CG_TT Cuts at: 1563 6792 7165 1563 Size: 5229 373 3063 Fragments arranged by size: 5229 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5456 5555 6728 8112 8380 1632 Size: 3824 99 1173 1384 268 1917 Fragments arranged by size: 3824 1917 1384 1173 268 99 AfeI AGC'GCT Cuts at: 4174 5677 4174 Size: 1503 7162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8468 AflIII A'CryG_T Cuts at: 1203 2739 7564 8541 1203 Size: 1536 4825 977 1327 Fragments arranged by size: 4825 1536 1327 977 AgeI A'CCGG_T Cuts at: 7496 7496 Size: 8665 AhdI GACnn_n'nnGTC Cuts at: 3562 5179 7394 3562 Size: 1617 2215 4833 Fragments arranged by size: 4833 2215 1617 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5694 5852 1184 Size: 4510 158 3997 Fragments arranged by size: 4510 3997 158 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4445 4477 4445 Size: 32 8633 AlwNI CAG_nnn'CTG Cuts at: 3328 3568 3712 5527 5610 5845 6408 7980 3328 Size: 240 144 1815 83 235 563 1572 4013 Fragments arranged by size: 4013 1815 1572 563 240 235 144 83 ApaI G_GGCC'C Cuts at: 2136 4040 4528 4666 5375 2136 Size: 1904 488 138 709 5426 Fragments arranged by size: 5426 1904 709 488 138 ApaLI G'TGCA_C Cuts at: 2267 6722 7878 2267 Size: 4455 1156 3054 Fragments arranged by size: 4455 3054 1156 ApoI r'AATT_y Cuts at: 1493 3118 5974 6200 6352 8461 1493 Size: 1625 2856 226 152 2109 1697 Fragments arranged by size: 2856 2109 1697 1625 226 152 AseI AT'TA_AT Cuts at: 160 1032 7219 160 Size: 872 6187 1606 Fragments arranged by size: 6187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8665 AvaI C'yCGr_G Cuts at: 1870 1943 2308 2372 2710 2725 2815 3098 3746 Size: 73 365 64 338 15 90 283 648 Cuts at: 3746 3952 4018 4148 4223 4520 4624 4714 5374 Size: 206 66 130 75 297 104 90 660 Cuts at: 5374 5886 5990 1870 Size: 512 104 4545 Fragments arranged by size: 4545 660 648 512 365 338 297 283 206 130 104 104 90 90 75 73 66 64 15 BamHI G'GATC_C Cuts at: 2149 5995 6362 2149 Size: 3846 367 4452 Fragments arranged by size: 4452 3846 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 2719 2819 3006 3313 Size: 325 217 627 756 176 100 187 307 Cuts at: 3313 3836 3851 3868 3994 4352 4748 4871 4993 Size: 523 15 17 126 358 396 123 122 Cuts at: 4993 5273 5296 5432 5740 5986 7441 618 Size: 280 23 136 308 246 1455 1842 Fragments arranged by size: 1842 1455 756 627 523 396 358 325 308 307 280 246 217 187 176 136 126 123 122 100 23 17 15 BanII G_rGCy'C Cuts at: 1391 2031 2136 2205 2594 2991 3002 4040 4167 Size: 640 105 69 389 397 11 1038 127 Cuts at: 4167 4430 4528 4631 4666 4868 4931 5375 5984 Size: 263 98 103 35 202 63 444 609 Cuts at: 5984 1391 Size: 4072 Fragments arranged by size: 4072 1038 640 609 444 397 389 263 202 127 105 103 98 69 63 35 11 BbeI G_GCGC'C Cuts at: 2723 2823 3317 3840 3872 3998 5277 5300 5436 Size: 100 494 523 32 126 1279 23 136 Cuts at: 5436 5744 2723 Size: 308 5644 Fragments arranged by size: 5644 1279 523 494 308 136 126 100 32 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7694 BbvCI CC'TCA_GC Cuts at: 3048 5812 3048 Size: 2764 5901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2378 2412 4042 4076 5780 5814 6856 Size: 34 441 34 1630 34 1704 34 1042 Cuts at: 6856 6890 1903 Size: 34 3678 Fragments arranged by size: 3678 1704 1630 1042 441 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3240 6557 7767 3240 Size: 3317 1210 4138 Fragments arranged by size: 4138 3317 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2406 2564 3100 Size: 107 122 71 631 605 734 158 536 Cuts at: 3100 3489 3765 3789 4236 5385 5530 5567 5576 Size: 389 276 24 447 1149 145 37 9 Cuts at: 5576 5905 7276 136 Size: 329 1371 1525 Fragments arranged by size: 1525 1371 1149 734 631 605 536 447 389 329 276 158 145 122 107 71 37 24 9 BglII A'GATC_T Cuts at: 8660 8660 Size: 8665 BlpI GC'TnA_GC Cuts at: 3732 6082 3732 Size: 2350 6315 Bme1580I G_kGCm'C Cuts at: 1790 2136 2271 3128 3167 3513 3854 4040 4083 Size: 346 135 857 39 346 341 186 43 Cuts at: 4083 4528 4666 4996 5375 5390 5901 6726 7882 Size: 445 138 330 379 15 511 825 1156 Cuts at: 7882 1790 Size: 2573 Fragments arranged by size: 2573 1156 857 825 511 445 379 346 346 341 330 186 138 135 43 39 15 BmgBI CAC'GTC Cuts at: 1318 3754 4785 5022 5869 1318 Size: 2436 1031 237 847 4114 Fragments arranged by size: 4114 2436 1031 847 237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2136 6396 7354 Size: 416 53 83 399 24 719 4260 958 Cuts at: 7354 442 Size: 1753 Fragments arranged by size: 4260 1753 958 719 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8665 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 5155 2740 Size: 2415 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2533 2614 2675 2986 3005 3266 4716 5420 Size: 1294 81 61 311 19 261 1450 704 Cuts at: 5420 5483 7325 1239 Size: 63 1842 2579 Fragments arranged by size: 2579 1842 1450 1294 704 311 261 81 63 61 19 Bpu10I CC'TnA_GC Cuts at: 3048 5812 3048 Size: 2764 5901 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6790 7655 7953 6790 Size: 865 298 7502 Fragments arranged by size: 7502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2072 3918 4589 4813 7328 882 Size: 617 573 1846 671 224 2515 2219 Fragments arranged by size: 2515 2219 1846 671 617 573 224 BsaAI yAC'GTr Cuts at: 493 1204 4078 4861 6456 493 Size: 711 2874 783 1595 2702 Fragments arranged by size: 2874 2702 1595 783 711 BsaBI GATnn'nnATC Cuts at: 8659 8659 Size: 8665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2720 2820 3314 3689 Size: 53 83 186 936 1187 100 494 375 Cuts at: 3689 3837 3844 3869 3995 5000 5274 5297 5433 Size: 148 7 25 126 1005 274 23 136 Cuts at: 5433 5741 5877 6472 6854 7489 275 Size: 308 136 595 382 635 1451 Fragments arranged by size: 1451 1187 1005 936 635 595 494 382 375 308 274 186 148 136 136 126 100 83 53 25 23 7 BsaWI w'CCGG_w Cuts at: 2081 3252 4900 7098 7496 7770 7917 8328 2081 Size: 1171 1648 2198 398 274 147 411 2418 Fragments arranged by size: 2418 2198 1648 1171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8440 Fragments arranged by size: 8440 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3291 3993 1186 Size: 2105 702 5858 Fragments arranged by size: 5858 2105 702 BseYI C'CCAG_C Cuts at: 2167 2874 2895 2913 3027 3147 3170 3206 3801 Size: 707 21 18 114 120 23 36 595 Cuts at: 3801 3986 4169 4304 4382 4423 4442 5599 5820 Size: 185 183 135 78 41 19 1157 221 Cuts at: 5820 7868 8364 2167 Size: 2048 496 2468 Fragments arranged by size: 2468 2048 1157 707 595 496 221 185 183 135 120 114 78 41 36 23 21 19 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 4272 5842 1163 Size: 573 636 1900 1570 3986 Fragments arranged by size: 3986 1900 1570 636 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2513 4261 4825 6115 6876 7904 Size: 275 186 512 1748 564 1290 761 1028 Cuts at: 7904 1540 Size: 2301 Fragments arranged by size: 2301 1748 1290 1028 761 564 512 275 186 BsiHKAI G_wGCw'C Cuts at: 2271 2469 5156 5642 5673 6726 6811 7882 2271 Size: 198 2687 486 31 1053 85 1071 3054 Fragments arranged by size: 3054 2687 1071 1053 486 198 85 31 BsmI GAATG_Cn' Cuts at: 5917 6176 6269 5917 Size: 259 93 8313 Fragments arranged by size: 8313 259 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4976 4976 Size: 8665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2136 2205 2271 2469 2594 2991 Size: 399 241 105 69 66 198 125 397 Cuts at: 2991 3002 3128 3167 3513 3854 4040 4083 4167 Size: 11 126 39 346 341 186 43 84 Cuts at: 4167 4430 4528 4631 4666 4868 4931 4996 5156 Size: 263 98 103 35 202 63 65 160 Cuts at: 5156 5375 5390 5642 5673 5901 5984 6726 6811 Size: 219 15 252 31 228 83 742 85 Cuts at: 6811 7882 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 346 341 263 252 241 228 219 202 198 186 160 126 125 105 103 98 85 84 83 69 66 65 63 43 39 35 31 15 11 BspEI T'CCGG_A Cuts at: 4900 8328 4900 Size: 3428 5237 BspHI T'CATG_A Cuts at: 3672 6554 8284 3672 Size: 2882 1730 4053 Fragments arranged by size: 4053 2882 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3936 5395 6020 844 Size: 3092 1459 625 3489 Fragments arranged by size: 3489 3092 1459 625 BsrBI CCG'CTC Cuts at: 1394 1823 6552 1394 Size: 429 4729 3507 Fragments arranged by size: 4729 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7160 7334 65 Size: 1183 5912 174 1396 Fragments arranged by size: 5912 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2563 3240 4188 4256 4260 4616 4804 Size: 110 753 677 948 68 4 356 188 Cuts at: 4804 5240 7309 7496 1700 Size: 436 2069 187 2869 Fragments arranged by size: 2869 2069 948 753 677 436 356 188 187 110 68 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8665 BssHII G'CGCG_C Cuts at: 1925 2163 2219 4413 1925 Size: 238 56 2194 6177 Fragments arranged by size: 6177 2194 238 56 BssSI C'ACGA_G Cuts at: 2380 5155 6725 7737 2380 Size: 2775 1570 1012 3308 Fragments arranged by size: 3308 2775 1570 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 3712 5610 794 Size: 1472 1446 1898 3849 Fragments arranged by size: 3849 1898 1472 1446 BstBI TT'CG_AA Cuts at: 7469 7469 Size: 8665 BstEII G'GTnAC_C Cuts at: 1184 5236 1184 Size: 4052 4613 BstXI CCAn_nnnn'nTGG Cuts at: 2801 4408 5650 2801 Size: 1607 1242 5816 Fragments arranged by size: 5816 1607 1242 BstYI r'GATC_y Cuts at: 1972 2149 5451 5466 5481 5995 6362 6747 6764 Size: 177 3302 15 15 514 367 385 17 Cuts at: 6764 8216 8302 8314 8660 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 3302 1977 1452 514 385 367 346 177 86 17 15 15 12 Bsu36I CC'TnA_GG Cuts at: 5572 5572 Size: 8665 BtgI C'CryG_G Cuts at: 513 1065 2299 2614 2660 5263 513 Size: 552 1234 315 46 2603 3915 Fragments arranged by size: 3915 2603 1234 552 315 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7926 Fragments arranged by size: 7926 683 56 BtsI GCAGTG_nn' Cuts at: 4617 5846 5854 6181 6975 6995 4617 Size: 1229 8 327 794 20 6287 Fragments arranged by size: 6287 1229 794 327 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2341 2376 529 Size: 35 1777 35 6818 Fragments arranged by size: 6818 1777 35 35 DraI TTT'AAA Cuts at: 5969 5969 Size: 8665 DraIII CAC_nnn'GTG Cuts at: 2560 2560 Size: 8665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7672 809 Size: 507 6356 1802 Fragments arranged by size: 6356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2224 2284 Size: 54 1425 50 275 18 223 171 60 Cuts at: 2284 2302 2826 3363 4258 4398 4465 4614 4822 Size: 18 524 537 895 140 67 149 208 Cuts at: 4822 6112 7001 8630 8 Size: 1290 889 1629 43 Fragments arranged by size: 1629 1425 1290 895 889 537 524 275 223 208 171 149 140 67 60 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4258 4822 6112 1537 Size: 275 2446 564 1290 4090 Fragments arranged by size: 4090 2446 1290 564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6595 1353 Size: 5242 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2489 3460 3903 3996 7252 7626 Size: 1064 138 900 971 443 93 3256 374 Cuts at: 7626 7772 387 Size: 146 1280 Fragments arranged by size: 3256 1280 1064 971 900 443 374 146 138 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2533 2614 2675 2986 3005 3266 4716 Size: 393 901 81 61 311 19 261 1450 Cuts at: 4716 5420 5456 5483 5555 6728 7325 8112 8380 Size: 704 36 27 72 1173 597 787 268 Cuts at: 8380 1239 Size: 1524 Fragments arranged by size: 1524 1450 1173 901 787 704 597 393 311 268 261 81 72 61 36 27 19 EcoNI CCTnn'n_nnAGG Cuts at: 5402 5402 Size: 8665 EcoO109I rG'GnC_Cy Cuts at: 2110 2132 2133 2376 2486 3034 3194 4036 4037 Size: 22 1 243 110 548 160 842 1 Cuts at: 4037 4524 4525 4662 4663 4670 5082 5371 5568 Size: 487 1 137 1 7 412 289 197 Cuts at: 5568 5704 2110 Size: 136 5071 Fragments arranged by size: 5071 842 548 487 412 289 243 197 160 137 136 110 22 7 1 1 1 1 EcoRI G'AATT_C Cuts at: 5974 6352 5974 Size: 378 8287 FspI TGC'GCA Cuts at: 7171 7171 Size: 8665 HaeII r_GCGC'y Cuts at: 1678 2723 2811 2823 3317 3447 3658 3840 3872 Size: 1045 88 12 494 130 211 182 32 Cuts at: 3872 3998 4176 4189 5018 5277 5300 5436 5634 Size: 126 178 13 829 259 23 136 198 Cuts at: 5634 5679 5744 7812 1678 Size: 45 65 2068 2531 Fragments arranged by size: 2531 2068 1045 829 494 259 211 198 182 178 136 130 126 88 65 45 32 23 13 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3548 3580 4874 4906 5165 Size: 32 135 32 1976 32 1294 32 259 Cuts at: 5165 5168 5197 5200 5212 5244 5639 5671 7306 Size: 3 29 3 12 32 395 32 1635 Cuts at: 7306 7338 7380 7412 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1976 1635 1294 395 259 135 42 32 32 32 32 32 32 32 32 29 12 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2038 2689 6009 6255 669 Size: 988 281 100 651 3320 246 3079 Fragments arranged by size: 3320 3079 988 651 281 246 100 HindIII A'AGCT_T Cuts at: 748 748 Size: 8665 HpaI GTT'AAC Cuts at: 6255 6255 Size: 8665 KasI G'GCGC_C Cuts at: 2719 2819 3313 3836 3868 3994 5273 5296 5432 Size: 100 494 523 32 126 1279 23 136 Cuts at: 5432 5740 2719 Size: 308 5644 Fragments arranged by size: 5644 1279 523 494 308 136 126 100 32 23 KpnI G_GTAC'C Cuts at: 5990 5990 Size: 8665 MfeI C'AATT_G Cuts at: 6264 6264 Size: 8665 MluI A'CGCG_T Cuts at: 8541 8541 Size: 8665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3136 5085 5955 7779 7963 1305 Size: 1831 1949 870 1824 184 2007 Fragments arranged by size: 2007 1949 1831 1824 870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 3365 10 Size: 54 1425 343 223 1310 5310 Fragments arranged by size: 5310 1425 1310 343 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 3359 6623 6982 7141 518 Size: 648 1029 1164 3264 359 159 2042 Fragments arranged by size: 3264 2042 1164 1029 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2581 2634 2770 2786 3174 3325 3411 3709 Size: 975 53 136 16 388 151 86 298 Cuts at: 3709 3965 4138 4281 4701 4908 5430 5607 5696 Size: 256 173 143 420 207 522 177 89 Cuts at: 5696 6081 6758 7906 8151 1606 Size: 385 677 1148 245 2120 Fragments arranged by size: 2120 1148 975 677 522 420 388 385 298 256 245 207 177 173 151 143 136 89 86 53 16 NaeI GCC'GGC Cuts at: 2565 4190 4258 4262 4618 2565 Size: 1625 68 4 356 6612 Fragments arranged by size: 6612 1625 356 68 4 NarI GG'CG_CC Cuts at: 2720 2820 3314 3837 3869 3995 5274 5297 5433 Size: 100 494 523 32 126 1279 23 136 Cuts at: 5433 5741 2720 Size: 308 5644 Fragments arranged by size: 5644 1279 523 494 308 136 126 100 32 23 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8113 NdeI CA'TA_TG Cuts at: 387 387 Size: 8665 NgoMIV G'CCGG_C Cuts at: 2563 4188 4256 4260 4616 2563 Size: 1625 68 4 356 6612 Fragments arranged by size: 6612 1625 356 68 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8665 NotI GC'GGCC_GC Cuts at: 6112 6112 Size: 8665 NspI r_CATG'y Cuts at: 1132 1229 2743 3262 3292 3436 5692 6023 7568 Size: 97 1514 519 30 144 2256 331 1545 Cuts at: 7568 1132 Size: 2229 Fragments arranged by size: 2256 2229 1545 1514 519 331 144 97 30 PasI CC'CwG_GG Cuts at: 1348 4738 4931 5745 1348 Size: 3390 193 814 4268 Fragments arranged by size: 4268 3390 814 193 PciI A'CATG_T Cuts at: 2739 7564 2739 Size: 4825 3840 PflMI CCAn_nnn'nTGG Cuts at: 1108 2653 3244 5461 5527 1108 Size: 1545 591 2217 66 4246 Fragments arranged by size: 4246 2217 1545 591 66 PfoI T'CCnGG_A Cuts at: 2523 4685 2523 Size: 2162 6503 PmeI GTTT'AAAC Cuts at: 5969 5969 Size: 8665 PmlI CAC'GTG Cuts at: 4078 4861 4078 Size: 783 7882 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6734 6766 8247 8279 6734 Size: 32 1481 32 7120 Fragments arranged by size: 7120 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4670 5082 4670 Size: 412 8253 PshAI GACnn'nnGTC Cuts at: 1613 4854 1613 Size: 3241 5424 PsiI TTA'TAA Cuts at: 6235 6235 Size: 8665 PspOMI G'GGCC_C Cuts at: 2132 4036 4524 4662 5371 2132 Size: 1904 488 138 709 5426 Fragments arranged by size: 5426 1904 709 488 138 PspXI vC'TCGA_Gb Cuts at: 5886 5886 Size: 8665 PstI C_TGCA'G Cuts at: 830 3186 3333 3543 4047 4107 4143 6017 830 Size: 2356 147 210 504 60 36 1874 3478 Fragments arranged by size: 3478 2356 1874 504 210 147 60 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8665 PvuII CAG'CTG Cuts at: 2770 4138 5607 2770 Size: 1368 1469 5828 Fragments arranged by size: 5828 1469 1368 RsrII CG'GwC_CG Cuts at: 5288 5288 Size: 8665 SalI G'TCGA_C Cuts at: 1936 2687 6007 1936 Size: 751 3320 4594 Fragments arranged by size: 4594 3320 751 SanDI GG'GwC_CC Cuts at: 5082 5082 Size: 8665 SbfI CC_TGCA'GG Cuts at: 3186 6017 3186 Size: 2831 5834 ScaI AGT'ACT Cuts at: 4889 6913 8625 4889 Size: 2024 1712 4929 Fragments arranged by size: 4929 2024 1712 SfcI C'TryA_G Cuts at: 826 1045 3182 3329 3539 4043 4103 4139 5489 Size: 219 2137 147 210 504 60 36 1350 Cuts at: 5489 6013 7148 7829 8020 826 Size: 524 1135 681 191 1471 Fragments arranged by size: 2137 1471 1350 1135 681 524 504 219 210 191 147 60 36 SfiI GGCCn_nnn'nGGCC Cuts at: 3765 3765 Size: 8665 SfoI GGC'GCC Cuts at: 2721 2821 3315 3838 3870 3996 5275 5298 5434 Size: 100 494 523 32 126 1279 23 136 Cuts at: 5434 5742 2721 Size: 308 5644 Fragments arranged by size: 5644 1279 523 494 308 136 126 100 32 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8665 SmaI CCC'GGG Cuts at: 1872 2310 2374 2712 2817 3100 3748 4150 4626 Size: 438 64 338 105 283 648 402 476 Cuts at: 4626 5376 5992 1872 Size: 750 616 4545 Fragments arranged by size: 4545 750 648 616 476 438 402 338 283 105 64 SmlI C'TyrA_G Cuts at: 820 1017 1943 5886 6769 7670 7932 820 Size: 197 926 3943 883 901 262 1553 Fragments arranged by size: 3943 1553 926 901 883 262 197 SnaBI TAC'GTA Cuts at: 493 6456 493 Size: 5963 2702 SpeI A'CTAG_T Cuts at: 152 5209 152 Size: 5057 3608 SphI G_CATG'C Cuts at: 1132 3262 3292 3436 6023 1132 Size: 2130 30 144 2587 3774 Fragments arranged by size: 3774 2587 2130 144 30 SrfI GCCC'GGGC Cuts at: 5376 5376 Size: 8665 SspI AAT'ATT Cuts at: 5 52 6589 5 Size: 47 6537 2081 Fragments arranged by size: 6537 2081 47 StuI AGG'CCT Cuts at: 1517 2430 3483 4128 5810 1517 Size: 913 1053 645 1682 4372 Fragments arranged by size: 4372 1682 1053 913 645 StyI C'CwwG_G Cuts at: 513 1065 2677 4449 5880 6104 513 Size: 552 1612 1772 1431 224 3074 Fragments arranged by size: 3074 1772 1612 1431 552 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 4613 5117 5305 5861 6692 6709 Size: 1255 534 2499 504 188 556 831 17 Cuts at: 6709 6862 7047 7492 325 Size: 153 185 445 1498 Fragments arranged by size: 2499 1498 1255 831 556 534 504 445 188 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4887 6911 8623 Size: 274 80 33 51 1443 2910 2024 1712 Cuts at: 8623 96 Size: 138 Fragments arranged by size: 2910 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3254 3286 8625 8657 3254 Size: 32 5339 32 3262 Fragments arranged by size: 5339 3262 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4833 1316 Size: 3517 5148 XbaI T'CTAG_A Cuts at: 6001 6001 Size: 8665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3241 4771 2011 Size: 1230 1530 5905 Fragments arranged by size: 5905 1530 1230 XhoI C'TCGA_G Cuts at: 1943 5886 1943 Size: 3943 4722 XmaI C'CCGG_G Cuts at: 1870 2308 2372 2710 2815 3098 3746 4148 4624 Size: 438 64 338 105 283 648 402 476 Cuts at: 4624 5374 5990 1870 Size: 750 616 4545 Fragments arranged by size: 4545 750 648 616 476 438 402 338 283 105 64 XmnI GAAnn'nnTTC Cuts at: 6794 6794 Size: 8665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6473 276 Size: 53 83 186 936 4939 2468 Fragments arranged by size: 4939 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PsrI SacI SacII SapI SexAI SwaI