(Circular) (Six-base) MAPSORT of: pFN21AE0070.seq Check: 285 from: 1 to: 6385 pFN21AE0070 6385 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2565 2565 Size: 6385 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3728 1937 Size: 1791 4594 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2197 2269 2540 4448 5832 6100 1632 Size: 565 72 271 1908 1384 268 1917 Fragments arranged by size: 1917 1908 1384 565 271 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 2424 3219 5284 6261 1203 Size: 1221 795 2065 977 1327 Fragments arranged by size: 2065 1327 1221 977 795 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 5114 5114 Size: 6385 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2924 3143 1184 Size: 1740 219 4426 Fragments arranged by size: 4426 1740 219 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2660 2692 2660 Size: 32 6353 AlwNI CAG_nnn'CTG Cuts at: 2437 2887 3365 4128 5700 2437 Size: 450 478 763 1572 3122 Fragments arranged by size: 3122 1572 763 478 450 ApaI G_GGCC'C Cuts at: 3297 3397 3428 3437 3526 3297 Size: 100 31 9 89 6156 Fragments arranged by size: 6156 100 89 31 9 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 3694 3920 4072 6181 1493 Size: 2201 226 152 2109 1697 Fragments arranged by size: 2201 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3662 4939 160 Size: 872 2630 1277 1606 Fragments arranged by size: 2630 1606 1277 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2110 2591 2708 2848 3421 3429 Size: 73 93 74 481 117 140 573 8 Cuts at: 3429 3710 1870 Size: 281 4545 Fragments arranged by size: 4545 573 481 281 140 117 93 74 73 8 AvrII C'CTAG_G Cuts at: 3322 3322 Size: 6385 BamHI G'GATC_C Cuts at: 3715 4082 3715 Size: 367 6018 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2279 2657 3002 3706 5161 Size: 325 217 627 492 378 345 704 1455 Cuts at: 5161 618 Size: 1842 Fragments arranged by size: 1842 1455 704 627 492 378 345 325 217 BanII G_rGCy'C Cuts at: 1391 2457 2847 2978 3027 3232 3297 3348 3397 Size: 1066 390 131 49 205 65 51 49 Cuts at: 3397 3428 3437 3526 3679 3704 1391 Size: 31 9 89 153 25 4072 Fragments arranged by size: 4072 1066 390 205 153 131 89 65 51 49 49 31 25 9 BbeI G_GCGC'C Cuts at: 2661 3006 2661 Size: 345 6040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2080 928 Size: 971 181 5233 Fragments arranged by size: 5233 971 181 BbvCI CC'TCA_GC Cuts at: 2009 2009 Size: 6385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4576 4610 1903 Size: 34 50 34 2555 34 3678 Fragments arranged by size: 3678 2555 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 3606 3615 1548 Size: 2058 9 4318 Fragments arranged by size: 4318 2058 9 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3333 3432 4996 Size: 107 122 71 631 605 1661 99 1564 Cuts at: 4996 136 Size: 1525 Fragments arranged by size: 1661 1564 1525 631 605 122 107 99 71 BglII A'GATC_T Cuts at: 6380 6380 Size: 6385 BlpI GC'TnA_GC Cuts at: 2586 2984 3802 2586 Size: 398 818 5169 Fragments arranged by size: 5169 818 398 Bme1580I G_kGCm'C Cuts at: 1790 3148 3264 3297 3397 3422 3428 3437 3526 Size: 1358 116 33 100 25 6 9 89 Cuts at: 3526 4446 5602 1790 Size: 920 1156 2573 Fragments arranged by size: 2573 1358 1156 920 116 100 89 33 25 9 6 BmgBI CAC'GTC Cuts at: 1318 2122 3220 1318 Size: 804 1098 4483 Fragments arranged by size: 4483 1098 804 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4116 5074 442 Size: 416 53 83 399 24 2699 958 1753 Fragments arranged by size: 2699 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2034 2034 Size: 6385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2396 2531 3011 3191 3221 5045 1239 Size: 1157 135 480 180 30 1824 2579 Fragments arranged by size: 2579 1824 1157 480 180 135 30 Bpu10I CC'TnA_GC Cuts at: 2009 2405 2942 2009 Size: 396 537 5452 Fragments arranged by size: 5452 537 396 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4510 5375 5673 4510 Size: 865 298 5222 Fragments arranged by size: 5222 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3197 5048 882 Size: 617 1698 1851 2219 Fragments arranged by size: 2219 1851 1698 617 BsaAI yAC'GTr Cuts at: 493 1204 4176 493 Size: 711 2972 2702 Fragments arranged by size: 2972 2702 711 BsaBI GATnn'nnATC Cuts at: 6379 6379 Size: 6385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2193 2658 3003 Size: 53 83 186 936 564 96 465 345 Cuts at: 3003 4192 4574 5209 275 Size: 1189 382 635 1451 Fragments arranged by size: 1451 1189 936 635 564 465 382 345 186 96 83 53 BsaWI w'CCGG_w Cuts at: 4818 5216 5490 5637 6048 4818 Size: 398 274 147 411 5155 Fragments arranged by size: 5155 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2399 2589 2619 2660 Size: 30 165 30 966 30 190 30 41 Cuts at: 2660 2690 3239 3269 1178 Size: 30 549 30 4294 Fragments arranged by size: 4294 966 549 190 165 41 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3454 1186 Size: 2268 4117 BseYI C'CCAG_C Cuts at: 2052 2904 3484 3525 5588 6084 2052 Size: 852 580 41 2063 496 2353 Fragments arranged by size: 2353 2063 852 580 496 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3026 1163 Size: 573 1290 4522 Fragments arranged by size: 4522 1290 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2722 3835 4596 5624 1540 Size: 275 186 721 1113 761 1028 2301 Fragments arranged by size: 2301 1113 1028 761 721 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 2111 2373 2457 2581 2847 2859 3027 3036 Size: 74 262 84 124 266 12 168 9 Cuts at: 3036 4446 4531 5602 2037 Size: 1410 85 1071 2820 Fragments arranged by size: 2820 1410 1071 266 262 168 124 85 84 74 12 9 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2111 2373 2457 2581 2847 2859 Size: 399 247 74 262 84 124 266 12 Cuts at: 2859 2978 3027 3036 3148 3232 3264 3297 3348 Size: 119 49 9 112 84 32 33 51 Cuts at: 3348 3397 3422 3428 3437 3526 3679 3704 4446 Size: 49 25 6 9 89 153 25 742 Cuts at: 4446 4531 5602 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 266 262 247 153 124 119 112 89 85 84 84 74 51 49 49 33 32 25 25 12 9 9 6 BspEI T'CCGG_A Cuts at: 6048 6048 Size: 6385 BspHI T'CATG_A Cuts at: 2241 4274 6004 2241 Size: 2033 1730 2622 Fragments arranged by size: 2622 2033 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 2565 3740 844 Size: 1610 111 1175 3489 Fragments arranged by size: 3489 1610 1175 111 BsrBI CCG'CTC Cuts at: 1394 1823 3388 4272 1394 Size: 429 1565 884 3507 Fragments arranged by size: 3507 1565 884 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4880 5054 65 Size: 1183 3632 174 1396 Fragments arranged by size: 3632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2721 3377 3529 5029 5216 1700 Size: 110 911 656 152 1500 187 2869 Fragments arranged by size: 2869 1500 911 656 187 152 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 2047 2126 2988 3130 1925 Size: 122 79 862 142 5180 Fragments arranged by size: 5180 862 142 122 79 BssSI C'ACGA_G Cuts at: 3329 4445 5457 3329 Size: 1116 1012 4257 Fragments arranged by size: 4257 1116 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 794 Size: 1376 5009 BstBI TT'CG_AA Cuts at: 3655 5189 3655 Size: 1534 4851 BstEII G'GTnAC_C Cuts at: 1184 3532 1184 Size: 2348 4037 BstXI CCAn_nnnn'nTGG Cuts at: 3017 3017 Size: 6385 BstYI r'GATC_y Cuts at: 1972 3715 4082 4467 4484 5936 6022 6034 6380 Size: 1743 367 385 17 1452 86 12 346 Cuts at: 6380 1972 Size: 1977 Fragments arranged by size: 1977 1743 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2312 2312 Size: 6385 BtgI C'CryG_G Cuts at: 513 1065 2188 2978 3350 3537 513 Size: 552 1123 790 372 187 3361 Fragments arranged by size: 3361 1123 790 552 372 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2686 487 Size: 683 56 1460 4186 Fragments arranged by size: 4186 1460 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3901 4695 4715 2111 Size: 1790 794 20 3781 Fragments arranged by size: 3781 1790 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6350 DraI TTT'AAA Cuts at: 3689 3689 Size: 6385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5392 809 Size: 507 4076 1802 Fragments arranged by size: 4076 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3643 3832 4721 Size: 54 1425 50 275 18 1813 189 889 Cuts at: 4721 6350 8 Size: 1629 43 Fragments arranged by size: 1813 1629 1425 889 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2180 2504 2864 3155 3336 4315 1353 Size: 827 324 360 291 181 979 3423 Fragments arranged by size: 3423 979 827 360 324 291 181 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2058 2254 4972 5346 5492 387 Size: 1064 138 469 196 2718 374 146 1280 Fragments arranged by size: 2718 1280 1064 469 374 196 146 138 EcoICRI GAG'CTC Cuts at: 2455 2845 3025 2455 Size: 390 180 5815 Fragments arranged by size: 5815 390 180 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2269 2396 2531 2540 3011 3191 Size: 393 565 72 127 135 9 471 180 Cuts at: 3191 3221 4448 5045 5832 6100 1239 Size: 30 1227 597 787 268 1524 Fragments arranged by size: 1524 1227 787 597 565 471 393 268 180 135 127 72 30 9 EcoNI CCTnn'n_nnAGG Cuts at: 2561 2561 Size: 6385 EcoO109I rG'GnC_Cy Cuts at: 2712 2875 3013 3250 3293 3294 3362 3394 3425 Size: 163 138 237 43 1 68 32 31 Cuts at: 3425 3433 2712 Size: 8 5664 Fragments arranged by size: 5664 237 163 138 68 43 32 31 8 1 EcoRI G'AATT_C Cuts at: 3694 4072 3694 Size: 378 6007 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 6385 FspI TGC'GCA Cuts at: 2912 3358 4891 2912 Size: 446 1533 4406 Fragments arranged by size: 4406 1533 446 HaeII r_GCGC'y Cuts at: 1678 2239 2661 3006 3277 5532 1678 Size: 561 422 345 271 2255 2531 Fragments arranged by size: 2531 2255 561 422 345 271 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2589 2621 3449 3481 5026 Size: 32 135 32 1017 32 828 32 1545 Cuts at: 5026 5058 5100 5132 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1545 1017 828 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2443 2817 3729 3975 669 Size: 988 281 505 374 912 246 3079 Fragments arranged by size: 3079 988 912 505 374 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6385 HpaI GTT'AAC Cuts at: 2443 3975 2443 Size: 1532 4853 KasI G'GCGC_C Cuts at: 2657 3002 2657 Size: 345 6040 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 2508 3072 5499 5683 1305 Size: 969 234 564 2427 184 2007 Fragments arranged by size: 2427 2007 969 564 234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3645 10 Size: 54 1425 343 1813 2750 Fragments arranged by size: 2750 1813 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4343 4702 4861 518 Size: 648 3177 359 159 2042 Fragments arranged by size: 3177 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2190 2217 2836 2908 2980 3199 3452 3801 Size: 584 27 619 72 72 219 253 349 Cuts at: 3801 4478 5626 5871 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 619 584 349 253 245 219 72 72 27 NaeI GCC'GGC Cuts at: 3379 3379 Size: 6385 NarI GG'CG_CC Cuts at: 2658 3003 2658 Size: 345 6040 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5833 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NgoMIV G'CCGG_C Cuts at: 3377 3377 Size: 6385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 2428 3743 5288 1132 Size: 97 1199 1315 1545 2229 Fragments arranged by size: 2229 1545 1315 1199 97 PasI CC'CwG_GG Cuts at: 1348 3365 3513 1348 Size: 2017 148 4220 Fragments arranged by size: 4220 2017 148 PciI A'CATG_T Cuts at: 2424 5284 2424 Size: 2860 3525 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6385 PfoI T'CCnGG_A Cuts at: 2091 2373 2091 Size: 282 6103 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2660 2692 4454 4486 5967 5999 2660 Size: 32 1762 32 1481 32 3046 Fragments arranged by size: 3046 1762 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3250 3250 Size: 6385 PshAI GACnn'nnGTC Cuts at: 1613 3248 1613 Size: 1635 4750 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PspOMI G'GGCC_C Cuts at: 3293 3393 3424 3433 3522 3293 Size: 100 31 9 89 6156 Fragments arranged by size: 6156 100 89 31 9 PstI C_TGCA'G Cuts at: 830 2202 2235 2451 2676 2883 3737 830 Size: 1372 33 216 225 207 854 3478 Fragments arranged by size: 3478 1372 854 225 216 207 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 2908 3199 2908 Size: 291 6094 SacI G_AGCT'C Cuts at: 2457 2847 3027 2457 Size: 390 180 5815 Fragments arranged by size: 5815 390 180 SacII CC_GC'GG Cuts at: 2191 2981 2191 Size: 790 5595 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SapI GCTCTTCn'nnn_ Cuts at: 2180 2864 3336 2180 Size: 684 472 5229 Fragments arranged by size: 5229 684 472 SbfI CC_TGCA'GG Cuts at: 2451 2883 3737 2451 Size: 432 854 5099 Fragments arranged by size: 5099 854 432 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SfcI C'TryA_G Cuts at: 826 1045 2198 2231 2447 2672 2879 3733 4868 Size: 219 1153 33 216 225 207 854 1135 Cuts at: 4868 5549 5740 826 Size: 681 191 1471 Fragments arranged by size: 1471 1153 1135 854 681 225 219 216 207 191 33 SfiI GGCCn_nnn'nGGCC Cuts at: 3432 3432 Size: 6385 SfoI GGC'GCC Cuts at: 2659 3004 2659 Size: 345 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6385 SmaI CCC'GGG Cuts at: 1872 2710 2850 3423 3431 3712 1872 Size: 838 140 573 8 281 4545 Fragments arranged by size: 4545 838 573 281 140 8 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 4489 5390 5652 820 Size: 197 926 648 1898 901 262 1553 Fragments arranged by size: 1898 1553 926 901 648 262 197 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 3743 1132 Size: 2611 3774 SrfI GCCC'GGGC Cuts at: 3423 3423 Size: 6385 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 2145 3327 1517 Size: 628 1182 4575 Fragments arranged by size: 4575 1182 628 StyI C'CwwG_G Cuts at: 513 1065 2562 3016 3322 3489 3679 3824 513 Size: 552 1497 454 306 167 190 145 3074 Fragments arranged by size: 3074 1497 552 454 306 190 167 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2918 3498 3505 4412 4429 4582 4767 Size: 1255 1338 580 7 907 17 153 185 Cuts at: 4767 5212 325 Size: 445 1498 Fragments arranged by size: 1498 1338 1255 907 580 445 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4631 6343 96 Size: 274 80 33 51 1443 2654 1712 138 Fragments arranged by size: 2654 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2326 2358 2681 2713 6345 6377 2326 Size: 32 323 32 3632 32 2334 Fragments arranged by size: 3632 2334 323 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6385 XbaI T'CTAG_A Cuts at: 3721 3721 Size: 6385 XhoI C'TCGA_G Cuts at: 1943 2591 1943 Size: 648 5737 XmaI C'CCGG_G Cuts at: 1870 2708 2848 3421 3429 3710 1870 Size: 838 140 573 8 281 4545 Fragments arranged by size: 4545 838 573 281 140 8 XmnI GAAnn'nnTTC Cuts at: 4514 4514 Size: 6385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SwaI XcmI