Restriction map for
pFN21AE0070
(Linear) (Six-base) MAPSORT of: KIEE0070.seq Check: 1280 from: 1 to: 1680
KIEE0070.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 560 1680
Size: 560 1120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 264 535 1680
Size: 192 72 271 1145
Fragments arranged by size:
1145 271 192 72
AflIII A'CryG_T
Cuts at: 0 419 1214 1680
Size: 419 795 466
Fragments arranged by size:
795 466 419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 919 1138 1680
Size: 919 219 542
Fragments arranged by size:
919 542 219
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 655 687 1680
Size: 655 32 993
Fragments arranged by size:
993 655 32
AlwNI CAG_nnn'CTG
Cuts at: 0 432 882 1360 1680
Size: 432 450 478 320
Fragments arranged by size:
478 450 432 320
ApaI G_GGCC'C
Cuts at: 0 1292 1392 1423 1432 1521 1680
Size: 1292 100 31 9 89 159
Fragments arranged by size:
1292 159 100 89 31 9
AseI AT'TA_AT
Cuts at: 0 1657 1680
Size: 1657 23
AvaI C'yCGr_G
Cuts at: 0 31 105 586 703 843 1416 1424 1680
Size: 31 74 481 117 140 573 8 256
Fragments arranged by size:
573 481 256 140 117 74 31 8
AvrII C'CTAG_G
Cuts at: 0 1317 1680
Size: 1317 363
BanI G'GyrC_C
Cuts at: 0 274 652 997 1680
Size: 274 378 345 683
Fragments arranged by size:
683 378 345 274
BanII G_rGCy'C
Cuts at: 0 452 842 973 1022 1227 1292 1343 1392
Size: 452 390 131 49 205 65 51 49
Cuts at: 1392 1423 1432 1521 1674 1680
Size: 31 9 89 153 6
Fragments arranged by size:
452 390 205 153 131 89 65 51
49 49 31 9 6
BbeI G_GCGC'C
Cuts at: 0 656 1001 1680
Size: 656 345 679
Fragments arranged by size:
679 656 345
BbsI GAAGACnn'nnnn_
Cuts at: 0 75 1680
Size: 75 1605
BbvCI CC'TCA_GC
Cuts at: 0 4 1680
Size: 4 1676
BclI T'GATC_A
Cuts at: 0 1601 1610 1680
Size: 1601 9 70
Fragments arranged by size:
1601 70 9
BglI GCCn_nnn'nGGC
Cuts at: 0 1328 1427 1680
Size: 1328 99 253
Fragments arranged by size:
1328 253 99
BlpI GC'TnA_GC
Cuts at: 0 581 979 1680
Size: 581 398 701
Fragments arranged by size:
701 581 398
Bme1580I G_kGCm'C
Cuts at: 0 1143 1259 1292 1392 1417 1423 1432 1521
Size: 1143 116 33 100 25 6 9 89
Cuts at: 1521 1680
Size: 159
Fragments arranged by size:
1143 159 116 100 89 33 25 9
6
BmgBI CAC'GTC
Cuts at: 0 117 1215 1680
Size: 117 1098 465
Fragments arranged by size:
1098 465 117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 29 1680
Size: 29 1651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 526 1006 1186 1216 1680
Size: 391 135 480 180 30 464
Fragments arranged by size:
480 464 391 180 135 30
Bpu10I CC'TnA_GC
Cuts at: 0 4 400 937 1680
Size: 4 396 537 743
Fragments arranged by size:
743 537 396 4
BsaI GGTCTCn'nnnn_
Cuts at: 0 1192 1680
Size: 1192 488
BsaHI Gr'CG_yC
Cuts at: 0 92 188 653 998 1680
Size: 92 96 465 345 682
Fragments arranged by size:
682 465 345 96 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 584 614 655 685 1234 1264
Size: 364 30 190 30 41 30 549 30
Cuts at: 1264 1680
Size: 416
Fragments arranged by size:
549 416 364 190 41 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1449 1680
Size: 1449 231
BseYI C'CCAG_C
Cuts at: 0 47 899 1479 1520 1680
Size: 47 852 580 41 160
Fragments arranged by size:
852 580 160 47 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1680
Size: 1021 659
BsiEI CG_ry'CG
Cuts at: 0 717 1680
Size: 717 963
BsiHKAI G_wGCw'C
Cuts at: 0 32 106 368 452 576 842 854 1022
Size: 32 74 262 84 124 266 12 168
Cuts at: 1022 1031 1680
Size: 9 649
Fragments arranged by size:
649 266 262 168 124 84 74 32
12 9
Bsp1286I G_dGCh'C
Cuts at: 0 32 106 368 452 576 842 854 973
Size: 32 74 262 84 124 266 12 119
Cuts at: 973 1022 1031 1143 1227 1259 1292 1343 1392
Size: 49 9 112 84 32 33 51 49
Cuts at: 1392 1417 1423 1432 1521 1674 1680
Size: 25 6 9 89 153 6
Fragments arranged by size:
266 262 153 124 119 112 89 84
84 74 51 49 49 33 32 32
25 12 9 9 6 6
BspHI T'CATG_A
Cuts at: 0 236 1680
Size: 236 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 560 1680
Size: 449 111 1120
Fragments arranged by size:
1120 449 111
BsrBI CCG'CTC
Cuts at: 0 1383 1680
Size: 1383 297
BsrFI r'CCGG_y
Cuts at: 0 716 1372 1524 1680
Size: 716 656 152 156
Fragments arranged by size:
716 656 156 152
BssHII G'CGCG_C
Cuts at: 0 42 121 983 1125 1680
Size: 42 79 862 142 555
Fragments arranged by size:
862 555 142 79 42
BssSI C'ACGA_G
Cuts at: 0 1324 1680
Size: 1324 356
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 1680
Size: 165 1515
BstBI TT'CG_AA
Cuts at: 0 1650 1680
Size: 1650 30
BstEII G'GTnAC_C
Cuts at: 0 1527 1680
Size: 1527 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1012 1680
Size: 1012 668
Bsu36I CC'TnA_GG
Cuts at: 0 307 1680
Size: 307 1373
BtgI C'CryG_G
Cuts at: 0 183 973 1345 1532 1680
Size: 183 790 372 187 148
Fragments arranged by size:
790 372 187 183 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 681 1680
Size: 681 999
BtsI GCAGTG_nn'
Cuts at: 0 106 1680
Size: 106 1574
EaeI y'GGCC_r
Cuts at: 0 1638 1680
Size: 1638 42
EarI CTCTTCn'nnn_
Cuts at: 0 175 499 859 1150 1331 1680
Size: 175 324 360 291 181 349
Fragments arranged by size:
360 349 324 291 181 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 53 249 1680
Size: 53 196 1431
Fragments arranged by size:
1431 196 53
EcoICRI GAG'CTC
Cuts at: 0 450 840 1020 1680
Size: 450 390 180 660
Fragments arranged by size:
660 450 390 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 264 391 526 535 1006 1186 1216
Size: 192 72 127 135 9 471 180 30
Cuts at: 1216 1680
Size: 464
Fragments arranged by size:
471 464 192 180 135 127 72 30
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 1680
Size: 556 1124
EcoO109I rG'GnC_Cy
Cuts at: 0 707 870 1008 1245 1288 1289 1357 1389
Size: 707 163 138 237 43 1 68 32
Cuts at: 1389 1420 1428 1680
Size: 31 8 252
Fragments arranged by size:
707 252 237 163 138 68 43 32
31 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 99 1680
Size: 99 1581
FspI TGC'GCA
Cuts at: 0 907 1353 1680
Size: 907 446 327
Fragments arranged by size:
907 446 327
HaeII r_GCGC'y
Cuts at: 0 234 656 1001 1272 1680
Size: 234 422 345 271 408
Fragments arranged by size:
422 408 345 271 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 584 616 1444 1476 1680
Size: 584 32 828 32 204
Fragments arranged by size:
828 584 204 32 32
HincII GTy'rAC
Cuts at: 0 438 812 1680
Size: 438 374 868
Fragments arranged by size:
868 438 374
HpaI GTT'AAC
Cuts at: 0 438 1680
Size: 438 1242
KasI G'GCGC_C
Cuts at: 0 652 997 1680
Size: 652 345 683
Fragments arranged by size:
683 652 345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 503 1067 1680
Size: 269 234 564 613
Fragments arranged by size:
613 564 269 234
MscI TGG'CCA
Cuts at: 0 1640 1680
Size: 1640 40
MspA1I CmG'CkG
Cuts at: 0 185 212 831 903 975 1194 1447 1680
Size: 185 27 619 72 72 219 253 233
Fragments arranged by size:
619 253 233 219 185 72 72 27
NaeI GCC'GGC
Cuts at: 0 1374 1680
Size: 1374 306
NarI GG'CG_CC
Cuts at: 0 653 998 1680
Size: 653 345 682
Fragments arranged by size:
682 653 345
NgoMIV G'CCGG_C
Cuts at: 0 1372 1680
Size: 1372 308
NspI r_CATG'y
Cuts at: 0 423 1680
Size: 423 1257
PasI CC'CwG_GG
Cuts at: 0 1360 1508 1680
Size: 1360 148 172
Fragments arranged by size:
1360 172 148
PciI A'CATG_T
Cuts at: 0 419 1680
Size: 419 1261
PfoI T'CCnGG_A
Cuts at: 0 86 368 1680
Size: 86 282 1312
Fragments arranged by size:
1312 282 86
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 1680
Size: 655 32 993
Fragments arranged by size:
993 655 32
PpuMI rG'GwC_Cy
Cuts at: 0 1245 1680
Size: 1245 435
PshAI GACnn'nnGTC
Cuts at: 0 1243 1680
Size: 1243 437
PspOMI G'GGCC_C
Cuts at: 0 1288 1388 1419 1428 1517 1680
Size: 1288 100 31 9 89 163
Fragments arranged by size:
1288 163 100 89 31 9
PstI C_TGCA'G
Cuts at: 0 197 230 446 671 878 1680
Size: 197 33 216 225 207 802
Fragments arranged by size:
802 225 216 207 197 33
PvuII CAG'CTG
Cuts at: 0 903 1194 1680
Size: 903 291 486
Fragments arranged by size:
903 486 291
SacI G_AGCT'C
Cuts at: 0 452 842 1022 1680
Size: 452 390 180 658
Fragments arranged by size:
658 452 390 180
SacII CC_GC'GG
Cuts at: 0 186 976 1680
Size: 186 790 704
Fragments arranged by size:
790 704 186
SapI GCTCTTCn'nnn_
Cuts at: 0 175 859 1331 1680
Size: 175 684 472 349
Fragments arranged by size:
684 472 349 175
SbfI CC_TGCA'GG
Cuts at: 0 446 878 1680
Size: 446 432 802
Fragments arranged by size:
802 446 432
SfcI C'TryA_G
Cuts at: 0 193 226 442 667 874 1680
Size: 193 33 216 225 207 806
Fragments arranged by size:
806 225 216 207 193 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1427 1680
Size: 1427 253
SfoI GGC'GCC
Cuts at: 0 654 999 1680
Size: 654 345 681
Fragments arranged by size:
681 654 345
SmaI CCC'GGG
Cuts at: 0 705 845 1418 1426 1680
Size: 705 140 573 8 254
Fragments arranged by size:
705 573 254 140 8
SmlI C'TyrA_G
Cuts at: 0 586 1680
Size: 586 1094
SrfI GCCC'GGGC
Cuts at: 0 1418 1680
Size: 1418 262
StuI AGG'CCT
Cuts at: 0 140 1322 1680
Size: 140 1182 358
Fragments arranged by size:
1182 358 140
StyI C'CwwG_G
Cuts at: 0 557 1011 1317 1484 1674 1680
Size: 557 454 306 167 190 6
Fragments arranged by size:
557 454 306 190 167 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 913 1493 1500 1680
Size: 913 580 7 180
Fragments arranged by size:
913 580 180 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 321 353 676 708 1680
Size: 321 32 323 32 972
Fragments arranged by size:
972 323 321 32 32
XhoI C'TCGA_G
Cuts at: 0 586 1680
Size: 586 1094
XmaI C'CCGG_G
Cuts at: 0 703 843 1416 1424 1680
Size: 703 140 573 8 256
Fragments arranged by size:
703 573 256 140 8
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApaI AseI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BclI
BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI Bsu36I BtgI BtgZI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII HpaI KasI MmeI MscI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SapI SbfI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaLI ApoI AscI AsiSI BaeI BamHI BcgI
BciVI BfrBI BglII BmrI BmtI BpuEI BsaAI BsaBI
BsaWI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BstYI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoRI
EcoRV FseI FspAI HindIII KpnI MfeI MluI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI
SanDI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TatI Tth111I XbaI XcmI XmnI ZraI