Restriction map for
pFN21AE0053
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# NO CUT ENZYMES ( 66 enzymes) #
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AclI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AvrII BbrPI
BbvCI BclI BlnI BsiWI BspDI
BspHI BspLU11I BssSI Bst1107I BstAPI
BstZ17I Bsu36I ClaI CpoI DrdI
Eam1105I Eco47III Eco81I FbaI HpaI
MfeI MluI MunI NdeI NheI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SbfI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 524 GGTACC G^GTACC
Alw44I 1440 GTGCAC G^TGCAC
ApaLI 1440 GTGCAC G^TGCAC
Asp718I 524 GGTACC G^GTACC
BcgI 3365 CGAAACTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2700 AGATCT A^GATCT
BlpI 2989 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2989 GCTGAGC GC^T[ACGT]AGC
BsaBI 2237 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstBI 1535 TTCGAA TT^CGAA
BstEII 2892 GGTGACC G^GT[ACGT]ACC
BstPI 2892 GGTGACC G^GT[ACGT]ACC
CelII 2989 GCTGAGC GC^T[ACGT]AGC
DraI 2436 TTTAAA TTT^AAA
DraIII 697 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2892 GGTGACC G^GT[ACGT]ACC
FseI 348 GGCCGGCC GGCCGG^CC
KpnI 524 GGTACC GGTAC^C
KspI 587 CCGCGG CCGC^GG
MamI 2237 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2646 CCATGG C^CATGG
NotI 934 GCGGCCGC GC^GGCCGC
NspV 1535 TTCGAA TT^CGAA
PflMI 773 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3247 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 587 CCGCGG CCGC^GG
SalI 4065 GTCGAC G^TCGAC
SapI 2225 GCTCTTC GCTCTTC(1/4)
SfuI 1535 TTCGAA TT^CGAA
SphI 1798 GCATGC GCATG^C
Van91I 773 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AatII 910 GACGTC GACGT^C
4210 GACGTC
AccI 3298 GTCTAC GT^[AC][GT]AC
4065 GTCGAC
AccIII 2963 TCCGGA T^CCGGA
3579 TCCGGA
AflII 1198 CTTAAG C^TTAAG
3723 CTTAAG
AviII 440 TGCGCA TGC^GCA
2453 TGCGCA
BamHI 2236 GGATCC G^GATCC
3889 GGATCC
BciVI 1844 GTATCC GTATCC(6/5)
2201 GTATCC
BfrI 1198 CTTAAG C^TTAAG
3723 CTTAAG
BglI 111 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
142 GCCATCGCGGC
BsaAI 547 TACGTG [CT]AC^GT[GA]
2975 TACGTG
BsaI 6 GGTCTC GGTCTC(1/5)
270 GGTCTC
BseAI 2963 TCCGGA T^CCGGA
3579 TCCGGA
Bsp1407I 1461 TGTACA T^GTACA
3542 TGTACA
BspEI 2963 TCCGGA T^CCGGA
3579 TCCGGA
BspMI 1663 ACCTGC ACCTGC(4/8)
3573 GCAGGT
BsrGI 1461 TGTACA T^GTACA
3542 TGTACA
BstXI 890 CCAGCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2205 CCAGTTTGATGG
EcoNI 894 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3082 CCTGAGTCAGG
EcoRI 1346 GAATTC G^AATTC
3583 GAATTC
EcoRV 3879 GATATC GAT^ATC
4420 GATATC
EcoT22I 3121 ATGCAT ATGCA^T
4352 ATGCAT
FspI 440 TGCGCA TGC^GCA
2453 TGCGCA
MroI 2963 TCCGGA T^CCGGA
3579 TCCGGA
NaeI 349 GCCGGC GCC^GGC
487 GCCGGC
NgoMIV 349 GCCGGC G^CCGGC
487 GCCGGC
NsiI 3121 ATGCAT ATGCA^T
4352 ATGCAT
PpuMI 2525 AGGACCC [GA]G^G[AT]CC[CT]
3510 GGGTCCC
PstI 2512 CTGCAG CTGCA^G
2995 CTGCAG
ScaI 3589 AGTACT AGT^ACT
3941 AGTACT
SmaI 301 CCCGGG CCC^GGG
2063 CCCGGG
SspBI 1461 TGTACA T^GTACA
3542 TGTACA
XmaCI 301 CCCGGG C^CCGGG
2063 CCCGGG
XmaI 301 CCCGGG C^CCGGG
2063 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII