Restriction map for
pFN21AE0050
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmAI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EagI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HapII HgaI Hin1I
HpaI HpaII KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 292 CCGC CCGC(-3/-1)
AflIII 907 ACATGT A^C[GA][CT]GT
Aor51HI 711 AGCGCT AGC^GCT
Asp700I 419 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 9 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 162 TGGCCA TGG^CCA
BbsI 6 GAAGAC GAAGAC(2/6)
BcgI 1259 GCAAGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 219 TGATCA T^GATCA
BpuAI 6 GAAGAC GAAGAC(2/6)
BsmFI 55 GGGAC GGGAC(10/14)
BspLU11I 907 ACATGT A^CATGT
BsrDI 59 CATTGC GCAATG(2/0)
CfoI 712 GCGC GCG^C
Cfr13I 389 GGCCC G^G[ACGT]CC
EaeI 162 TGGCCA [CT]^GGCC[GA]
Eco47III 711 AGCGCT AGC^GCT
FbaI 219 TGATCA T^GATCA
HaeII 711 AGCGCT [GA]GCGC^[CT]
HhaI 712 GCGC GCG^C
HinP1I 712 GCGC G^CGC
HincII 18 GTCAAC GT[CT]^[GA]AC
HindII 18 GTCAAC GT[CT]^[GA]AC
HindIII 267 AAGCTT A^AGCTT
HphI 640 GGTGA GGTGA(8/7)
MaeII 10 ACGT A^CGT
MluNI 162 TGGCCA TGG^CCA
MscI 162 TGGCCA TGG^CCA
MwoI 285 GCCTGAACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 9 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau96I 389 GGCCC G^G[ACGT]CC
SfaNI 279 GCATC GCATC(5/9)
SphI 228 GCATGC GCATG^C
Tth111I 9 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 419 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 491 GTATAC GT^[AC][GT]AC
1372 GTATAC
AcsI 91 GAATTT [GA]^AATT[CT]
377 GAATTT
AfaI 206 GTAC GT^AC
463 GTAC
AlwI 45 GATCC GGATC(4/5)
262 GGATC
ApoI 91 GAATTT [GA]^AATT[CT]
377 GAATTT
BbvI 169 GCAGC GCAGC(8/12)
518 GCAGC
BssKI 1116 CCTGG ^CC[ACGT]GG
1200 CCTGG
Bst1107I 491 GTATAC GTA^TAC
1372 GTATAC
BstNI 1116 CCTGG CC^[AT]GG
1200 CCTGG
BstZ17I 491 GTATAC GTA^TAC
1372 GTATAC
DdeI 357 CTCAG C^T[ACGT]AG
552 CTTAG
DrdI 9 GACGTTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
68 GACAAGCAAGTC
EcoRII 1116 CCTGG ^CC[AT]GG
1200 CCTGG
Fnu4HI 169 GCAGC GC^[ACGT]GC
518 GCAGC
ItaI 169 GCAGC GC^[ACGT]GC
518 GCAGC
MvaI 1116 CCTGG CC^[AT]GG
1200 CCTGG
NspI 228 GCATGC [GA]CATG^[CT]
907 ACATGT
RsaI 206 GTAC GT^AC
463 GTAC
ScrFI 1116 CCTGG CC^[ACGT]GG
1200 CCTGG
TaqI 131 TCGA T^CGA
936 TCGA
TseI 169 GCAGC G^C[AT]GC
518 GCAGC
TthHB8I 131 TCGA T^CGA
936 TCGA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AhdI AluI AspEI BpmI BsrI
BstF5I Cac8I DpnII Eam1105I EarI
FokI HaeIII HinfI Ksp632I MaeIII
MboI MboII MnlI MseI MslI
NdeII NlaIII Sau3AI TfiI Tru9I
Tsp45I Tsp509I TspRI