(Circular) (Six-base) MAPSORT of: pFN21AE0050.seq Check: 5849 from: 1 to: 6112 pFN21AE0050 6112 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2497 3378 3455 1937 Size: 560 881 77 4594 Fragments arranged by size: 4594 881 560 77 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 3340 4175 5559 5827 1632 Size: 702 1006 835 1384 268 1917 Fragments arranged by size: 1917 1384 1006 835 702 268 AfeI AGC'GCT Cuts at: 2718 2718 Size: 6112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 2912 5011 5988 1203 Size: 1709 2099 977 1327 Fragments arranged by size: 2099 1709 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 2685 3273 3357 4841 2685 Size: 588 84 1484 3956 Fragments arranged by size: 3956 1484 588 84 AleI CACnn'nnGTG Cuts at: 1166 2643 1166 Size: 1477 4635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 3855 5427 3855 Size: 1572 4540 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 2096 2382 3421 3647 3799 5908 1493 Size: 603 286 1039 226 152 2109 1697 Fragments arranged by size: 2109 1697 1039 603 286 226 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 3437 1870 Size: 73 1494 4545 Fragments arranged by size: 4545 1494 73 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3433 4888 618 Size: 325 217 627 1646 1455 1842 Fragments arranged by size: 1842 1646 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3431 1391 Size: 2040 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2018 928 Size: 971 119 5022 Fragments arranged by size: 5022 971 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3253 3287 4303 4337 1903 Size: 34 1316 34 1016 34 3678 Fragments arranged by size: 3678 1316 1016 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 2224 1548 Size: 676 5436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4723 136 Size: 107 122 71 631 605 3051 1525 Fragments arranged by size: 3051 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 4173 5329 1790 Size: 2383 1156 2573 Fragments arranged by size: 2573 2383 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3843 4801 442 Size: 416 53 83 399 24 2426 958 1753 Fragments arranged by size: 2426 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2748 2832 2916 3000 3168 3252 3336 4772 Size: 1509 84 84 84 168 84 84 1436 Cuts at: 4772 1239 Size: 2579 Fragments arranged by size: 2579 1509 1436 168 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4237 5102 5400 4237 Size: 865 298 4949 Fragments arranged by size: 4949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4775 882 Size: 617 3276 2219 Fragments arranged by size: 3276 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3919 4301 4936 275 Size: 53 83 186 936 2386 382 635 1451 Fragments arranged by size: 2386 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4545 4943 5217 5364 5775 4545 Size: 398 274 147 411 4882 Fragments arranged by size: 4882 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5887 Fragments arranged by size: 5887 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6112 BseYI C'CCAG_C Cuts at: 5315 5811 5315 Size: 496 5616 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4173 4258 5329 4173 Size: 85 1071 4956 Fragments arranged by size: 4956 1071 85 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3431 4173 4258 5329 1391 Size: 399 1641 742 85 1071 2174 Fragments arranged by size: 2174 1641 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 4001 5731 4001 Size: 1730 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3467 844 Size: 2623 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2063 4607 4781 65 Size: 1183 815 2544 174 1396 Fragments arranged by size: 2544 1396 1183 815 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4756 4943 1700 Size: 110 2946 187 2869 Fragments arranged by size: 2946 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 4172 5184 4172 Size: 1012 5100 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6112 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstYI r'GATC_y Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107 Size: 1470 367 385 17 1452 86 12 346 Cuts at: 6107 1972 Size: 1977 Fragments arranged by size: 1977 1470 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2498 3379 2498 Size: 881 5231 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5560 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3270 487 Size: 683 56 2044 3329 Fragments arranged by size: 3329 2044 683 56 BtsI GCAGTG_nn' Cuts at: 3628 4422 4442 3628 Size: 794 20 5298 Fragments arranged by size: 5298 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2020 2079 5119 809 Size: 507 704 59 3040 1802 Fragments arranged by size: 3040 1802 704 507 59 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 3559 4448 Size: 54 1425 50 275 18 337 1392 889 Cuts at: 4448 6077 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1392 889 337 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2556 2708 2997 4042 1353 Size: 1203 152 289 1045 3423 Fragments arranged by size: 3423 1203 1045 289 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2748 2832 2916 3000 3168 3252 Size: 393 702 414 84 84 84 168 84 Cuts at: 3252 3336 3340 4175 4772 5559 5827 1239 Size: 84 4 835 597 787 268 1524 Fragments arranged by size: 1524 835 787 702 597 414 393 268 168 84 84 84 84 84 4 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2279 2279 Size: 6112 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 2720 5259 1678 Size: 1042 2539 2531 Fragments arranged by size: 2539 2531 1042 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2671 2703 3259 3291 3343 Size: 32 135 32 1099 32 556 32 52 Cuts at: 3343 3375 4753 4785 4827 4859 1373 Size: 32 1378 32 42 32 2626 Fragments arranged by size: 2626 1378 1099 556 135 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2025 3456 3702 669 Size: 988 281 87 1431 246 3079 Fragments arranged by size: 3079 1431 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 2272 748 Size: 1524 4588 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5226 5410 1305 Size: 3921 184 2007 Fragments arranged by size: 3921 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 10 Size: 54 1425 343 337 3953 Fragments arranged by size: 3953 1425 343 337 54 MslI CAynn'nnrTG Cuts at: 518 1166 2458 2643 2827 3163 4070 4429 4588 Size: 648 1292 185 184 336 907 359 159 Cuts at: 4588 518 Size: 2042 Fragments arranged by size: 2042 1292 907 648 359 336 185 184 159 MspA1I CmG'CkG Cuts at: 1606 3528 4205 5353 5598 1606 Size: 1922 677 1148 245 2120 Fragments arranged by size: 2120 1922 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5560 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2237 2916 3470 5015 1132 Size: 97 1008 679 554 1545 2229 Fragments arranged by size: 2229 1545 1008 679 554 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 2912 5011 2912 Size: 2099 4013 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6112 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3293 3682 3293 Size: 389 5723 PstI C_TGCA'G Cuts at: 830 3464 830 Size: 2634 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 3460 4595 5276 5467 826 Size: 219 2415 1135 681 191 1471 Fragments arranged by size: 2415 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4216 5117 5379 820 Size: 197 926 2273 901 262 1553 Fragments arranged by size: 2273 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 2237 3470 1132 Size: 1105 1233 3774 Fragments arranged by size: 3774 1233 1105 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 3551 513 Size: 552 2486 3074 Fragments arranged by size: 3074 2486 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 3263 4139 4156 4309 4494 4939 Size: 1255 614 1069 876 17 153 185 445 Cuts at: 4939 325 Size: 1498 Fragments arranged by size: 1498 1255 1069 876 614 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2210 2467 4358 Size: 274 80 33 51 1443 233 257 1891 Cuts at: 4358 6070 96 Size: 1712 138 Fragments arranged by size: 1891 1712 1443 274 257 233 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3046 3078 6072 6104 3046 Size: 32 2994 32 3054 Fragments arranged by size: 3054 2994 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2017 1316 Size: 701 5411 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 2428 4241 2428 Size: 1813 4299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI