Restriction map for
pFN21AB5605
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AocI ApoI AscI AseI
AsnI Asp700I AvrII BalI BamHI
BbsI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI DraI DrdI EarI Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI HindIII HpaI Ksp632I MamI
MboII MfeI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NotI NsiI NspV PacI PaeR7I
PflMI PmeI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI SapI
ScaI SfiI SfuI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 1378 GGTACC G^GTACC
AflIII 761 ACGCGT A^C[GA][CT]GT
AgeI 962 ACCGGT A^CCGGT
AhdI 1230 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 669 GTGCAC G^TGCAC
Aor51HI 1013 AGCGCT AGC^GCT
ApaLI 669 GTGCAC G^TGCAC
Asp718I 1378 GGTACC G^GTACC
AspEI 1230 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 852 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1616 TGCGCA TGC^GCA
BbrPI 1693 CACGTG CAC^GTG
BbvCI 427 GCTGAGG CCTCAGC(-5/-2)
BglII 149 AGATCT A^GATCT
BpmI 991 CTGGAG CTGGAG(16/14)
BsaAI 1693 CACGTG [CT]AC^GT[GA]
BsaI 931 GAGACC GGTCTC(1/5)
BsaWI 962 ACCGGT [AT]^CCGG[AT]
BsmBI 564 CGTCTC CGTCTC(1/5)
BsmFI 728 GGGAC GGGAC(10/14)
BsmI 1539 GCATTC GAATGC(1/-1)
BsrDI 767 GCAATG GCAATG(2/0)
BssHII 23 GCGCGC G^CGCGC
BstYI 149 AGATCT [GA]^GATC[CT]
CpoI 1699 CGGACCG CG^G[AT]CCG
DdeI 428 CTGAG C^T[ACGT]AG
DraIII 1028 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1230 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1013 AGCGCT AGC^GCT
FseI 36 GGCCGGCC GGCCGG^CC
FspI 1616 TGCGCA TGC^GCA
HincII 1021 GTCAAC GT[CT]^[GA]AC
HindII 1021 GTCAAC GT[CT]^[GA]AC
HphI 572 TCACC GGTGA(8/7)
KpnI 1378 GGTACC GGTAC^C
MflI 149 AGATCT [GA]^GATC[CT]
MluI 761 ACGCGT A^CGCGT
NheI 1267 GCTAGC G^CTAGC
NlaIII 924 CATG CATG^
NruI 1568 TCGCGA TCG^CGA
NspI 923 GCATGT [GA]CATG^[CT]
PflFI 852 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 962 ACCGGT A^CCGGT
PmaCI 1693 CACGTG CAC^GTG
PmlI 1693 CACGTG CAC^GTG
PpuMI 8 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 271 CAGCTG CAG^CTG
RsrII 1699 CGGACCG CG^G[AT]CCG
SexAI 1067 ACCTGGT A^CC[AT]GGT
SmaI 117 CCCGGG CCC^GGG
Tsp45I 1439 GTGAC ^GT[GC]AC
Tth111I 852 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1637 CCAGGGCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 149 AGATCT [GA]^GATC[CT]
XmaCI 117 CCCGGG C^CCGGG
XmaI 117 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 774 GATCC GGATC(4/5)
260 GGATC
AlwNI 613 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1135 CAGCCCCTG
AvaI 117 CCCGGG C^[CT]CG[GA]G
304 CTCGGG
BfaI 1151 CTAG C^TAG
1268 CTAG
BsgI 837 CTGCAC GTGCAG(16/14)
1314 CTGCAC
BsiEI 950 CGGCCG CG[GA][CT]^CG
1523 CGGCCG
BsoBI 117 CCCGGG C^[CT]CG[GA]G
304 CTCGGG
BstAPI 80 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1689 GCAGCACGTGC
EagI 950 CGGCCG C^GGCCG
1523 CGGCCG
EclXI 950 CGGCCG C^GGCCG
1523 CGGCCG
Eco52I 950 CGGCCG C^GGCCG
1523 CGGCCG
EcoT14I 18 CCTTGG C^C[AT][AT]GG
1673 CCAAGG
MaeI 1151 CTAG C^TAG
1268 CTAG
MaeII 974 ACGT A^CGT
1694 ACGT
MaeIII 331 GTTAC ^GT[ACGT]AC
1439 GTGAC
SbfI 521 CCTGCAGG CCTGCA^GG
816 CCTGCAGG
SgrAI 247 CGCCGGCG C[GA]^CCGG[CT]G
961 CACCGGTG
Sse8387I 521 CCTGCAGG CCTGCA^GG
816 CCTGCAGG
StyI 18 CCTTGG C^C[AT][AT]GG
1673 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaII BanI BanII
BbeI BbvI BcgI BcnI BglI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI KspI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIV PleI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI TaqI TseI TspRI TthHB8I