Restriction map for
pFN21AB5884
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AlwI AlwNI Aor51HI ApoI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbeI BcgI
BclI BfaI BfrI BlnI BsaBI
BsaI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 555 GGTACC G^GTACC
AccI 1000 GTAGAC GT^[AC][GT]AC
AgeI 281 ACCGGT A^CCGGT
Alw44I 304 GTGCAC G^TGCAC
ApaLI 304 GTGCAC G^TGCAC
Asp718I 555 GGTACC G^GTACC
BalI 416 TGGCCA TGG^CCA
BbsI 328 GTCTTC GAAGAC(2/6)
BbvCI 201 GCTGAGG CCTCAGC(-5/-2)
BciVI 1214 GTATCC GTATCC(6/5)
BglII 602 AGATCT A^GATCT
BpuAI 328 GTCTTC GAAGAC(2/6)
BsaWI 281 ACCGGT [AT]^CCGG[AT]
BsiEI 651 CGGCCG CG[GA][CT]^CG
BsmAI 847 GAGAC GTCTC(1/5)
Bsp1407I 1393 TGTACA T^GTACA
BspMI 686 ACCTGC ACCTGC(4/8)
BsrDI 752 GCAATG GCAATG(2/0)
BsrGI 1393 TGTACA T^GTACA
BssHII 400 GCGCGC G^CGCGC
BstAPI 599 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 602 AGATCT [GA]^GATC[CT]
EagI 651 CGGCCG C^GGCCG
EclXI 651 CGGCCG C^GGCCG
Eco52I 651 CGGCCG C^GGCCG
KpnI 555 GGTACC GGTAC^C
MaeIII 425 GTGAC ^GT[ACGT]AC
MflI 602 AGATCT [GA]^GATC[CT]
MluNI 416 TGGCCA TGG^CCA
MscI 416 TGGCCA TGG^CCA
MslI 342 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PinAI 281 ACCGGT A^CCGGT
SacI 6 GAGCTC GAGCT^C
SapI 69 GCTCTTC GCTCTTC(1/4)
SspBI 1393 TGTACA T^GTACA
TaqI 74 TCGA T^CGA
Tsp45I 425 GTGAC ^GT[GC]AC
TthHB8I 74 TCGA T^CGA
XhoII 602 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 427 GACGCC G[GA]^CG[CT]C
1302 GGCGTC
AhdI 916 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACCTGGCGTC
AspEI 916 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACCTGGCGTC
BbrPI 250 CACGTG CAC^GTG
1161 CACGTG
BsaAI 250 CACGTG [CT]AC^GT[GA]
1161 CACGTG
BsaHI 427 GACGCC G[GA]^CG[CT]C
1302 GGCGTC
BsrBI 76 GAGCGG CCGCTC(-3/-3)
1356 GAGCGG
BssSI 438 CTCGTG CACGAG(-5/-1)
676 CTCGTG
BstXI 137 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1017 CCAGGTGCCTGG
DraIII 523 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1180 CACCCAGTG
Eam1105I 916 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACCTGGCGTC
EarI 70 CTCTTC CTCTTC(1/4)
334 CTCTTC
EcoT14I 22 CCAAGG C^C[AT][AT]GG
1452 CCTTGG
HgaI 427 GACGC GACGC(5/10)
1303 GCGTC
Hin1I 427 GACGCC G[GA]^CG[CT]C
1302 GGCGTC
HphI 1266 GGTGA GGTGA(8/7)
461 TCACC
Ksp632I 70 CTCTTC CTCTTC(1/4)
334 CTCTTC
PflMI 189 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1179 CCACCCAGTGG
PmaCI 250 CACGTG CAC^GTG
1161 CACGTG
PmlI 250 CACGTG CAC^GTG
1161 CACGTG
PstI 11 CTGCAG CTGCA^G
1045 CTGCAG
SfaNI 310 GATGC GCATC(5/9)
872 GCATC
StuI 672 AGGCCT AGG^CCT
1084 AGGCCT
StyI 22 CCAAGG C^C[AT][AT]GG
1452 CCTTGG
Van91I 189 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1179 CCACCCAGTGG
XcmI 500 CCACATTCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1017 CCAGGTGCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SmaI TseI TspRI
XmaCI XmaI