(Circular) (Six-base) MAPSORT of: pFN21AE0006.seq Check: 5196 from: 1 to: 6268 pFN21AE0006 6268 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2076 3456 3611 1937 Size: 139 1380 155 4594 Fragments arranged by size: 4594 1380 155 139 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4331 5715 5983 1632 Size: 2699 1384 268 1917 Fragments arranged by size: 2699 1917 1384 268 AfeI AGC'GCT Cuts at: 2221 2221 Size: 6268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6071 AflIII A'CryG_T Cuts at: 1203 5167 6144 1203 Size: 3964 977 1327 Fragments arranged by size: 3964 1327 977 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 3107 1166 Size: 1941 4327 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2598 2826 1184 Size: 1414 228 4626 Fragments arranged by size: 4626 1414 228 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 2823 2855 2714 Size: 32 77 32 6127 Fragments arranged by size: 6127 77 32 32 AlwNI CAG_nnn'CTG Cuts at: 2944 4011 5583 2944 Size: 1067 1572 3629 Fragments arranged by size: 3629 1572 1067 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 2111 2162 3146 3250 3577 3803 3955 6064 Size: 618 51 984 104 327 226 152 2109 Cuts at: 6064 1493 Size: 1697 Fragments arranged by size: 2109 1697 984 618 327 226 152 104 51 AseI AT'TA_AT Cuts at: 160 1032 2469 4822 160 Size: 872 1437 2353 1606 Fragments arranged by size: 2353 1606 1437 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 2659 3593 1870 Size: 73 716 934 4545 Fragments arranged by size: 4545 934 716 73 BamHI G'GATC_C Cuts at: 2838 3598 3965 2838 Size: 760 367 5141 Fragments arranged by size: 5141 760 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3589 5044 618 Size: 325 217 627 1802 1455 1842 Fragments arranged by size: 1842 1802 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3587 1391 Size: 2196 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3053 3087 4459 4493 1903 Size: 34 1116 34 1372 34 3678 Fragments arranged by size: 3678 1372 1116 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 1548 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4879 136 Size: 107 122 71 631 605 3207 1525 Fragments arranged by size: 3207 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 3685 3685 Size: 6268 Bme1580I G_kGCm'C Cuts at: 1790 4329 5485 1790 Size: 2539 1156 2573 Fragments arranged by size: 2573 2539 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3999 4957 442 Size: 416 53 83 399 24 2582 958 1753 Fragments arranged by size: 2582 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2238 2238 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 4928 1239 Size: 827 2862 2579 Fragments arranged by size: 2862 2579 827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2702 2996 4393 5258 5556 2702 Size: 294 1397 865 298 3414 Fragments arranged by size: 3414 1397 865 298 294 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2589 2671 4931 882 Size: 617 1090 82 2260 2219 Fragments arranged by size: 2260 2219 1090 617 82 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 3279 6262 3279 Size: 2983 3285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4075 4457 5092 275 Size: 53 83 186 936 2542 382 635 1451 Fragments arranged by size: 2542 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4701 5099 5373 5520 5931 4701 Size: 398 274 147 411 5038 Fragments arranged by size: 5038 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2991 3021 1178 Size: 30 165 30 1588 30 4425 Fragments arranged by size: 4425 1588 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2230 2999 1186 Size: 877 167 769 4455 Fragments arranged by size: 4455 877 769 167 BseYI C'CCAG_C Cuts at: 2199 2571 5471 5967 2199 Size: 372 2900 496 2500 Fragments arranged by size: 2900 2500 496 372 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5695 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4329 4414 5485 4329 Size: 85 1071 5112 Fragments arranged by size: 5112 1071 85 BsmI GAATG_Cn' Cuts at: 3779 3872 3779 Size: 93 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3587 4329 4414 5485 1391 Size: 399 1797 742 85 1071 2174 Fragments arranged by size: 2174 1797 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 4157 5887 4157 Size: 1730 4538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3623 844 Size: 2779 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2659 3189 4763 4937 65 Size: 1183 1411 530 1574 174 1396 Fragments arranged by size: 1574 1411 1396 1183 530 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4912 5099 1700 Size: 110 3102 187 2869 Fragments arranged by size: 3102 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3421 96 Size: 3325 2943 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6268 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstYI r'GATC_y Cuts at: 1972 2779 2838 3003 3598 3965 4350 4367 5819 Size: 807 59 165 595 367 385 17 1452 Cuts at: 5819 5905 5917 6263 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 807 595 385 367 346 165 86 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2395 513 Size: 552 939 186 205 4386 Fragments arranged by size: 4386 939 552 205 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5529 Fragments arranged by size: 5529 683 56 BtsI GCAGTG_nn' Cuts at: 2710 3031 3784 4578 4598 2710 Size: 321 753 794 20 4380 Fragments arranged by size: 4380 794 753 321 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 3572 3572 Size: 6268 DraIII CAC_nnn'GTG Cuts at: 3217 3217 Size: 6268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 3715 4604 Size: 54 1425 50 275 18 498 1387 889 Cuts at: 4604 6233 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1387 889 498 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2816 3033 4198 1353 Size: 1463 217 1165 3423 Fragments arranged by size: 3423 1463 1165 217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2843 4855 5229 5375 387 Size: 1064 138 1254 2012 374 146 1280 Fragments arranged by size: 2012 1280 1254 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 4331 4928 5715 5983 1239 Size: 393 434 2265 597 787 268 1524 Fragments arranged by size: 2265 1524 787 597 434 393 268 EcoO109I rG'GnC_Cy Cuts at: 2195 2195 Size: 6268 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 EcoRV GAT'ATC Cuts at: 2527 2527 Size: 6268 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 2223 5415 1678 Size: 545 3192 2531 Fragments arranged by size: 3192 2531 545 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2764 2796 2846 2878 3152 Size: 32 135 32 1192 32 50 32 274 Cuts at: 3152 3184 4909 4941 4983 5015 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 1192 274 135 50 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2634 3612 3858 669 Size: 988 281 696 978 246 3079 Fragments arranged by size: 3079 988 978 696 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6268 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 3867 3867 Size: 6268 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2943 5382 5566 1305 Size: 1638 2439 184 2007 Fragments arranged by size: 2439 2007 1638 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 10 Size: 54 1425 343 498 3948 Fragments arranged by size: 3948 1425 498 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 3065 3107 4226 4585 4744 518 Size: 648 1122 777 42 1119 359 159 2042 Fragments arranged by size: 2042 1122 1119 777 648 359 159 42 MspA1I CmG'CkG Cuts at: 1606 2203 2629 3684 4361 5509 5754 1606 Size: 597 426 1055 677 1148 245 2120 Fragments arranged by size: 2120 1148 1055 677 597 426 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4777 Fragments arranged by size: 4777 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 2338 2483 2878 3626 5171 1132 Size: 97 1109 145 395 748 1545 2229 Fragments arranged by size: 2229 1545 1109 748 395 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6268 PciI A'CATG_T Cuts at: 5167 5167 Size: 6268 PflMI CCAn_nnn'nTGG Cuts at: 1108 2760 3319 1108 Size: 1652 559 4057 Fragments arranged by size: 4057 1652 559 PfoI T'CCnGG_A Cuts at: 3255 3312 3255 Size: 57 6211 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2195 2195 Size: 6268 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 2495 3838 2495 Size: 1343 4925 PstI C_TGCA'G Cuts at: 830 2940 3079 3274 3620 830 Size: 2110 139 195 346 3478 Fragments arranged by size: 3478 2110 346 195 139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SanDI GG'GwC_CC Cuts at: 2195 2195 Size: 6268 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 2976 4516 6228 2976 Size: 1540 1712 3016 Fragments arranged by size: 3016 1712 1540 SfcI C'TryA_G Cuts at: 826 1045 2073 2419 2512 2602 2936 3075 3270 Size: 219 1028 346 93 90 334 139 195 Cuts at: 3270 3616 4751 5432 5623 826 Size: 346 1135 681 191 1471 Fragments arranged by size: 1471 1135 1028 681 346 346 334 219 195 191 139 93 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 3011 4372 5273 5535 820 Size: 197 926 774 294 1361 901 262 1553 Fragments arranged by size: 1553 1361 926 901 774 294 262 197 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 2483 3626 1132 Size: 1351 1143 3774 Fragments arranged by size: 3774 1351 1143 SspI AAT'ATT Cuts at: 5 52 3503 4192 5 Size: 47 3451 689 2081 Fragments arranged by size: 3451 2081 689 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6268 StyI C'CwwG_G Cuts at: 513 1065 2004 3707 513 Size: 552 939 1703 3074 Fragments arranged by size: 3074 1703 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4295 4312 4465 4650 5095 325 Size: 1255 2715 17 153 185 445 1498 Fragments arranged by size: 2715 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2924 2974 3421 Size: 274 80 33 51 1443 947 50 447 Cuts at: 3421 4514 6226 96 Size: 1093 1712 138 Fragments arranged by size: 1712 1443 1093 947 447 274 138 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6228 6260 6228 Size: 32 6236 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6268 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 2895 4397 2895 Size: 1502 4766 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI