(Circular) (Six-base) MAPSORT of: pFN21AB9969.seq Check: 864 from: 1 to: 6361 pFN21AB9969 6361 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2123 2731 2123 Size: 608 5753 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4171 278 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3704 1937 Size: 1767 4594 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 4488 4861 1563 Size: 2925 373 3063 Fragments arranged by size: 3063 2925 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2306 2384 2447 3584 4424 5808 6076 Size: 633 41 78 63 1137 840 1384 268 Cuts at: 6076 1632 Size: 1917 Fragments arranged by size: 1917 1384 1137 840 633 268 78 63 41 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6164 AflIII A'CryG_T Cuts at: 1203 2538 5260 6237 1203 Size: 1335 2722 977 1327 Fragments arranged by size: 2722 1335 1327 977 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 5090 5090 Size: 6361 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2672 1184 Size: 1488 4873 AlwNI CAG_nnn'CTG Cuts at: 2671 3508 4104 5676 2671 Size: 837 596 1572 3356 Fragments arranged by size: 3356 1572 837 596 ApaLI G'TGCA_C Cuts at: 4418 5574 4418 Size: 1156 5205 ApoI r'AATT_y Cuts at: 1493 3670 3896 4048 6157 1493 Size: 2177 226 152 2109 1697 Fragments arranged by size: 2177 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 2846 3094 3300 3429 3686 1870 Size: 73 903 248 206 129 257 4545 Fragments arranged by size: 4545 903 257 248 206 129 73 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2756 2839 2854 3220 3419 Size: 325 217 627 969 83 15 366 199 Cuts at: 3419 3682 5137 618 Size: 263 1455 1842 Fragments arranged by size: 1842 1455 969 627 366 325 263 217 199 83 15 BanII G_rGCy'C Cuts at: 1391 2210 3101 3680 1391 Size: 819 891 579 4072 Fragments arranged by size: 4072 891 819 579 BbeI G_GCGC'C Cuts at: 2760 3423 2760 Size: 663 5698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3269 928 Size: 971 1370 4020 Fragments arranged by size: 4020 1370 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4552 4586 1903 Size: 34 2615 34 3678 Fragments arranged by size: 3678 2615 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4253 5463 4253 Size: 1210 5151 BclI T'GATC_A Cuts at: 1548 1548 Size: 6361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2680 2780 2922 Size: 107 122 71 631 605 1008 100 142 Cuts at: 2922 3431 3449 4972 136 Size: 509 18 1523 1525 Fragments arranged by size: 1525 1523 1008 631 605 509 142 122 107 100 71 18 BglII A'GATC_T Cuts at: 6356 6356 Size: 6361 BlpI GC'TnA_GC Cuts at: 2421 3002 3778 2421 Size: 581 776 5004 Fragments arranged by size: 5004 776 581 Bme1580I G_kGCm'C Cuts at: 1790 2844 3492 4422 5578 1790 Size: 1054 648 930 1156 2573 Fragments arranged by size: 2573 1156 1054 930 648 BmgBI CAC'GTC Cuts at: 1318 3556 1318 Size: 2238 4123 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4092 5050 442 Size: 416 53 83 399 24 2675 958 1753 Fragments arranged by size: 2675 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2246 2356 2246 Size: 110 6251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2326 2339 2549 3137 5021 1239 Size: 1087 13 210 588 1884 2579 Fragments arranged by size: 2579 1884 1087 588 210 13 Bpu10I CC'TnA_GC Cuts at: 3185 3185 Size: 6361 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4486 5351 5649 4486 Size: 865 298 5198 Fragments arranged by size: 5198 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5024 882 Size: 617 3525 2219 Fragments arranged by size: 3525 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2539 4152 493 Size: 711 1335 1613 2702 Fragments arranged by size: 2702 1613 1335 711 BsaBI GATnn'nnATC Cuts at: 6355 6355 Size: 6361 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2400 2502 2757 Size: 53 83 186 936 633 234 102 255 Cuts at: 2757 2907 3420 4168 4550 5185 275 Size: 150 513 748 382 635 1451 Fragments arranged by size: 1451 936 748 635 633 513 382 255 234 186 150 102 83 53 BsaWI w'CCGG_w Cuts at: 2088 4794 5192 5466 5613 6024 2088 Size: 2706 398 274 147 411 2425 Fragments arranged by size: 2706 2425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2477 2507 2591 2621 2999 Size: 30 165 30 1074 30 84 30 378 Cuts at: 2999 3029 1178 Size: 30 4510 Fragments arranged by size: 4510 1074 378 165 84 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2585 2970 1186 Size: 853 546 385 4577 Fragments arranged by size: 4577 853 546 385 BseYI C'CCAG_C Cuts at: 2702 2768 2961 3164 5564 6060 2702 Size: 66 193 203 2400 496 3003 Fragments arranged by size: 3003 2400 496 203 193 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2747 3317 1163 Size: 573 1011 570 4207 Fragments arranged by size: 4207 1011 573 570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3811 4572 5600 1540 Size: 275 186 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2891 3101 3627 4422 4507 5578 2891 Size: 210 526 795 85 1071 3674 Fragments arranged by size: 3674 1071 795 526 210 85 BsmI GAATG_Cn' Cuts at: 3872 3965 3872 Size: 93 6268 BsmBI CGTCTCn'nnnn_ Cuts at: 2217 2217 Size: 6361 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2844 2891 3101 3492 3627 3680 Size: 399 420 634 47 210 391 135 53 Cuts at: 3680 4422 4507 5578 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 634 420 399 391 210 135 85 53 47 BspEI T'CCGG_A Cuts at: 2088 6024 2088 Size: 3936 2425 BspHI T'CATG_A Cuts at: 2898 4250 5980 2898 Size: 1352 1730 3279 Fragments arranged by size: 3279 1730 1352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2123 2731 3716 844 Size: 1279 608 985 3489 Fragments arranged by size: 3489 1279 985 608 BsrBI CCG'CTC Cuts at: 1394 1823 4248 1394 Size: 429 2425 3507 Fragments arranged by size: 3507 2425 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3130 4856 5030 65 Size: 1183 1882 1726 174 1396 Fragments arranged by size: 1882 1726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 2836 3110 5005 5192 1700 Size: 110 224 802 274 1895 187 2869 Fragments arranged by size: 2869 1895 802 274 224 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6361 BssHII G'CGCG_C Cuts at: 1925 2719 1925 Size: 794 5567 BssSI C'ACGA_G Cuts at: 4421 5433 4421 Size: 1012 5349 BstAPI GCAn_nnn'nTGC Cuts at: 794 2671 2734 794 Size: 1877 63 4421 Fragments arranged by size: 4421 1877 63 BstBI TT'CG_AA Cuts at: 5165 5165 Size: 6361 BstEII G'GTnAC_C Cuts at: 1184 3200 1184 Size: 2016 4345 BstXI CCAn_nnnn'nTGG Cuts at: 3164 3164 Size: 6361 BstYI r'GATC_y Cuts at: 1972 2259 2709 3691 4058 4443 4460 5912 5998 Size: 287 450 982 367 385 17 1452 86 Cuts at: 5998 6010 6356 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 982 450 385 367 346 287 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3225 3225 Size: 6361 BtgI C'CryG_G Cuts at: 513 1065 2103 2613 2628 2939 3102 513 Size: 552 1038 510 15 311 163 3772 Fragments arranged by size: 3772 1038 552 510 311 163 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2083 3249 3330 487 Size: 683 56 857 1166 81 3518 Fragments arranged by size: 3518 1166 857 683 81 56 BtsI GCAGTG_nn' Cuts at: 2612 3328 3877 4671 4691 2612 Size: 716 549 794 20 4282 Fragments arranged by size: 4282 794 716 549 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6326 DraI TTT'AAA Cuts at: 3665 3665 Size: 6361 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5368 809 Size: 507 4052 1802 Fragments arranged by size: 4052 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2616 2745 Size: 54 1425 50 275 18 360 426 129 Cuts at: 2745 3127 3396 3450 3483 3808 4697 6326 8 Size: 382 269 54 33 325 889 1629 43 Fragments arranged by size: 1629 1425 889 426 382 360 325 275 269 129 54 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3808 1537 Size: 275 1996 4090 Fragments arranged by size: 4090 1996 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2438 3350 3652 4291 1353 Size: 884 201 912 302 639 3423 Fragments arranged by size: 3423 912 884 639 302 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2884 3589 4948 5322 5468 387 Size: 1064 138 1295 705 1359 374 146 1280 Fragments arranged by size: 1359 1295 1280 1064 705 374 146 138 EcoICRI GAG'CTC Cuts at: 3099 3099 Size: 6361 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2306 2326 2339 2384 2447 2549 Size: 393 633 41 20 13 45 63 102 Cuts at: 2549 3137 3584 4424 5021 5808 6076 1239 Size: 588 447 840 597 787 268 1524 Fragments arranged by size: 1524 840 787 633 597 588 447 393 268 102 63 45 41 20 13 EcoNI CCTnn'n_nnAGG Cuts at: 3383 3383 Size: 6361 EcoO109I rG'GnC_Cy Cuts at: 3471 3505 3471 Size: 34 6327 EcoRI G'AATT_C Cuts at: 3670 4048 3670 Size: 378 5983 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3166 3166 Size: 6361 FspI TGC'GCA Cuts at: 3035 4867 3035 Size: 1832 4529 HaeII r_GCGC'y Cuts at: 1678 2584 2760 2775 3423 3642 5508 1678 Size: 906 176 15 648 219 1866 2531 Fragments arranged by size: 2531 1866 906 648 219 176 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2251 2283 2999 3031 3073 Size: 32 135 32 679 32 716 32 42 Cuts at: 3073 3105 3526 3558 5002 5034 5076 5108 1373 Size: 32 421 32 1444 32 42 32 2626 Fragments arranged by size: 2626 1444 716 679 421 135 42 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3705 3951 669 Size: 988 281 1767 246 3079 Fragments arranged by size: 3079 1767 988 281 246 HindIII A'AGCT_T Cuts at: 748 2432 748 Size: 1684 4677 HpaI GTT'AAC Cuts at: 3951 3951 Size: 6361 KasI G'GCGC_C Cuts at: 2756 3419 2756 Size: 663 5698 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 3960 3960 Size: 6361 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2568 2817 2832 3042 5475 5659 1305 Size: 1263 249 15 210 2433 184 2007 Fragments arranged by size: 2433 2007 1263 249 210 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 2618 2747 3398 10 Size: 54 1425 343 360 426 129 651 2973 Fragments arranged by size: 2973 1425 651 426 360 343 129 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 3630 4319 4678 4837 518 Size: 648 936 1528 689 359 159 2042 Fragments arranged by size: 2042 1528 936 689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 2317 2630 2677 3456 3562 3777 4454 Size: 666 45 313 47 779 106 215 677 Cuts at: 4454 5602 5847 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 779 677 666 313 245 215 106 47 45 NaeI GCC'GGC Cuts at: 3112 3112 Size: 6361 NarI GG'CG_CC Cuts at: 2757 3420 2757 Size: 663 5698 NcoI C'CATG_G Cuts at: 513 1065 2103 3102 513 Size: 552 1038 999 3772 Fragments arranged by size: 3772 1038 999 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6361 NgoMIV G'CCGG_C Cuts at: 3110 3110 Size: 6361 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NspI r_CATG'y Cuts at: 1132 1229 3719 5264 1132 Size: 97 2490 1545 2229 Fragments arranged by size: 2490 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3502 1348 Size: 2154 4207 PciI A'CATG_T Cuts at: 5260 5260 Size: 6361 PflMI CCAn_nnn'nTGG Cuts at: 1108 3508 1108 Size: 2400 3961 PfoI T'CCnGG_A Cuts at: 2712 3378 2712 Size: 666 5695 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4430 4462 5943 5975 4430 Size: 32 1481 32 4816 Fragments arranged by size: 4816 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2412 1613 Size: 799 5562 PsiI TTA'TAA Cuts at: 3931 3931 Size: 6361 PspXI vC'TCGA_Gb Cuts at: 3094 3094 Size: 6361 PstI C_TGCA'G Cuts at: 830 2493 3348 3713 830 Size: 1663 855 365 3478 Fragments arranged by size: 3478 1663 855 365 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 PvuII CAG'CTG Cuts at: 2272 2317 3562 2272 Size: 45 1245 5071 Fragments arranged by size: 5071 1245 45 SacI G_AGCT'C Cuts at: 3101 3101 Size: 6361 SacII CC_GC'GG Cuts at: 2631 2631 Size: 6361 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SapI GCTCTTCn'nnn_ Cuts at: 2438 3350 2438 Size: 912 5449 SbfI CC_TGCA'GG Cuts at: 3713 3713 Size: 6361 ScaI AGT'ACT Cuts at: 2474 4609 6321 2474 Size: 2135 1712 2514 Fragments arranged by size: 2514 2135 1712 SexAI A'CCwGG_T Cuts at: 2796 2796 Size: 6361 SfcI C'TryA_G Cuts at: 826 1045 2489 2932 3344 3475 3709 4844 5525 Size: 219 1444 443 412 131 234 1135 681 Cuts at: 5525 5716 826 Size: 191 1471 Fragments arranged by size: 1471 1444 1135 681 443 412 234 219 191 131 SfoI GGC'GCC Cuts at: 2758 3421 2758 Size: 663 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 3302 3431 3688 1872 Size: 1430 129 257 4545 Fragments arranged by size: 4545 1430 257 129 SmlI C'TyrA_G Cuts at: 820 1017 1943 3094 4465 5366 5628 820 Size: 197 926 1151 1371 901 262 1553 Fragments arranged by size: 1553 1371 1151 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4152 493 Size: 3659 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6361 SphI G_CATG'C Cuts at: 1132 3719 1132 Size: 2587 3774 SspI AAT'ATT Cuts at: 5 52 4285 5 Size: 47 4233 2081 Fragments arranged by size: 4233 2081 47 StuI AGG'CCT Cuts at: 1517 2690 1517 Size: 1173 5188 StyI C'CwwG_G Cuts at: 513 1065 2103 2643 3102 3604 3800 513 Size: 552 1038 540 459 502 196 3074 Fragments arranged by size: 3074 1038 552 540 502 459 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2908 4388 4405 4558 4743 5188 325 Size: 1255 1328 1480 17 153 185 445 1498 Fragments arranged by size: 1498 1480 1328 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2493 4607 Size: 274 80 33 51 1443 495 21 2114 Cuts at: 4607 6319 96 Size: 1712 138 Fragments arranged by size: 2114 1712 1443 495 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2591 2623 6321 6353 2591 Size: 32 3698 32 2599 Fragments arranged by size: 3698 2599 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6361 XbaI T'CTAG_A Cuts at: 3697 3697 Size: 6361 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2794 2794 Size: 6361 XhoI C'TCGA_G Cuts at: 1943 3094 1943 Size: 1151 5210 XmaI C'CCGG_G Cuts at: 1870 3300 3429 3686 1870 Size: 1430 129 257 4545 Fragments arranged by size: 4545 1430 257 129 XmnI GAAnn'nnTTC Cuts at: 4490 4490 Size: 6361 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4169 276 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SanDI SfiI SrfI SwaI