Restriction map for
pFN21AB9969
(Linear) (Six-base) MAPSORT of: KIBB9969.seq Check: 3611 from: 1 to: 1656
KIBB9969.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 118 726 1656
Size: 118 608 930
Fragments arranged by size:
930 608 118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 301 379 442 1579 1656
Size: 260 41 78 63 1137 77
Fragments arranged by size:
1137 260 78 77 63 41
AflIII A'CryG_T
Cuts at: 0 533 1656
Size: 533 1123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 667 1656
Size: 667 989
AlwNI CAG_nnn'CTG
Cuts at: 0 666 1503 1656
Size: 666 837 153
Fragments arranged by size:
837 666 153
AvaI C'yCGr_G
Cuts at: 0 841 1089 1295 1424 1656
Size: 841 248 206 129 232
Fragments arranged by size:
841 248 232 206 129
BanI G'GyrC_C
Cuts at: 0 751 834 849 1215 1414 1656
Size: 751 83 15 366 199 242
Fragments arranged by size:
751 366 242 199 83 15
BanII G_rGCy'C
Cuts at: 0 205 1096 1656
Size: 205 891 560
Fragments arranged by size:
891 560 205
BbeI G_GCGC'C
Cuts at: 0 755 1418 1656
Size: 755 663 238
Fragments arranged by size:
755 663 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 1264 1656
Size: 1264 392
BglI GCCn_nnn'nGGC
Cuts at: 0 675 775 917 1426 1444 1656
Size: 675 100 142 509 18 212
Fragments arranged by size:
675 509 212 142 100 18
BlpI GC'TnA_GC
Cuts at: 0 416 997 1656
Size: 416 581 659
Fragments arranged by size:
659 581 416
Bme1580I G_kGCm'C
Cuts at: 0 839 1487 1656
Size: 839 648 169
Fragments arranged by size:
839 648 169
BmgBI CAC'GTC
Cuts at: 0 1551 1656
Size: 1551 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 241 351 1656
Size: 241 110 1305
Fragments arranged by size:
1305 241 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 334 544 1132 1656
Size: 321 13 210 588 524
Fragments arranged by size:
588 524 321 210 13
Bpu10I CC'TnA_GC
Cuts at: 0 1180 1656
Size: 1180 476
BsaAI yAC'GTr
Cuts at: 0 534 1656
Size: 534 1122
BsaHI Gr'CG_yC
Cuts at: 0 161 395 497 752 902 1415 1656
Size: 161 234 102 255 150 513 241
Fragments arranged by size:
513 255 241 234 161 150 102
BsaWI w'CCGG_w
Cuts at: 0 83 1656
Size: 83 1573
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 472 502 586 616 994 1024 1656
Size: 472 30 84 30 378 30 632
Fragments arranged by size:
632 472 378 84 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 580 965 1656
Size: 34 546 385 691
Fragments arranged by size:
691 546 385 34
BseYI C'CCAG_C
Cuts at: 0 697 763 956 1159 1656
Size: 697 66 193 203 497
Fragments arranged by size:
697 497 203 193 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1312 1656
Size: 742 570 344
Fragments arranged by size:
742 570 344
BsiHKAI G_wGCw'C
Cuts at: 0 886 1096 1622 1656
Size: 886 210 526 34
Fragments arranged by size:
886 526 210 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 212 1656
Size: 212 1444
Bsp1286I G_dGCh'C
Cuts at: 0 205 839 886 1096 1487 1622 1656
Size: 205 634 47 210 391 135 34
Fragments arranged by size:
634 391 210 205 135 47 34
BspEI T'CCGG_A
Cuts at: 0 83 1656
Size: 83 1573
BspHI T'CATG_A
Cuts at: 0 893 1656
Size: 893 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 118 726 1656
Size: 118 608 930
Fragments arranged by size:
930 608 118
BsrDI GCAATG_nn'
Cuts at: 0 1125 1656
Size: 1125 531
BsrFI r'CCGG_y
Cuts at: 0 29 831 1105 1656
Size: 29 802 274 551
Fragments arranged by size:
802 551 274 29
BssHII G'CGCG_C
Cuts at: 0 714 1656
Size: 714 942
BstAPI GCAn_nnn'nTGC
Cuts at: 0 666 729 1656
Size: 666 63 927
Fragments arranged by size:
927 666 63
BstEII G'GTnAC_C
Cuts at: 0 1195 1656
Size: 1195 461
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1159 1656
Size: 1159 497
BstYI r'GATC_y
Cuts at: 0 254 704 1656
Size: 254 450 952
Fragments arranged by size:
952 450 254
Bsu36I CC'TnA_GG
Cuts at: 0 1220 1656
Size: 1220 436
BtgI C'CryG_G
Cuts at: 0 98 608 623 934 1097 1656
Size: 98 510 15 311 163 559
Fragments arranged by size:
559 510 311 163 98 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 78 1244 1325 1656
Size: 78 1166 81 331
Fragments arranged by size:
1166 331 81 78
BtsI GCAGTG_nn'
Cuts at: 0 607 1323 1656
Size: 607 716 333
Fragments arranged by size:
716 607 333
EaeI y'GGCC_r
Cuts at: 0 185 611 740 1122 1391 1445 1478 1656
Size: 185 426 129 382 269 54 33 178
Fragments arranged by size:
426 382 269 185 178 129 54 33
EarI CTCTTCn'nnn_
Cuts at: 0 232 433 1345 1656
Size: 232 201 912 311
Fragments arranged by size:
912 311 232 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 879 1584 1656
Size: 879 705 72
Fragments arranged by size:
879 705 72
EcoICRI GAG'CTC
Cuts at: 0 1094 1656
Size: 1094 562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 301 321 334 379 442 544 1132
Size: 260 41 20 13 45 63 102 588
Cuts at: 1132 1579 1656
Size: 447 77
Fragments arranged by size:
588 447 260 102 77 63 45 41
20 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1378 1656
Size: 1378 278
EcoO109I rG'GnC_Cy
Cuts at: 0 1466 1500 1656
Size: 1466 34 156
Fragments arranged by size:
1466 156 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1161 1656
Size: 1161 495
FspI TGC'GCA
Cuts at: 0 1030 1656
Size: 1030 626
HaeII r_GCGC'y
Cuts at: 0 579 755 770 1418 1637 1656
Size: 579 176 15 648 219 19
Fragments arranged by size:
648 579 219 176 19 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 994 1026 1068 1100 1521 1553
Size: 246 32 716 32 42 32 421 32
Cuts at: 1553 1656
Size: 103
Fragments arranged by size:
716 421 246 103 42 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 427 1656
Size: 427 1229
KasI G'GCGC_C
Cuts at: 0 751 1414 1656
Size: 751 663 242
Fragments arranged by size:
751 663 242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 812 827 1037 1656
Size: 563 249 15 210 619
Fragments arranged by size:
619 563 249 210 15
MscI TGG'CCA
Cuts at: 0 187 613 742 1393 1656
Size: 187 426 129 651 263
Fragments arranged by size:
651 426 263 187 129
MslI CAynn'nnrTG
Cuts at: 0 97 1625 1656
Size: 97 1528 31
Fragments arranged by size:
1528 97 31
MspA1I CmG'CkG
Cuts at: 0 267 312 625 672 1451 1557 1656
Size: 267 45 313 47 779 106 99
Fragments arranged by size:
779 313 267 106 99 47 45
NaeI GCC'GGC
Cuts at: 0 1107 1656
Size: 1107 549
NarI GG'CG_CC
Cuts at: 0 752 1415 1656
Size: 752 663 241
Fragments arranged by size:
752 663 241
NcoI C'CATG_G
Cuts at: 0 98 1097 1656
Size: 98 999 559
Fragments arranged by size:
999 559 98
NgoMIV G'CCGG_C
Cuts at: 0 1105 1656
Size: 1105 551
PasI CC'CwG_GG
Cuts at: 0 1497 1656
Size: 1497 159
PflMI CCAn_nnn'nTGG
Cuts at: 0 1503 1656
Size: 1503 153
PfoI T'CCnGG_A
Cuts at: 0 707 1373 1656
Size: 707 666 283
Fragments arranged by size:
707 666 283
PshAI GACnn'nnGTC
Cuts at: 0 407 1656
Size: 407 1249
PspXI vC'TCGA_Gb
Cuts at: 0 1089 1656
Size: 1089 567
PstI C_TGCA'G
Cuts at: 0 488 1343 1656
Size: 488 855 313
Fragments arranged by size:
855 488 313
PvuII CAG'CTG
Cuts at: 0 267 312 1557 1656
Size: 267 45 1245 99
Fragments arranged by size:
1245 267 99 45
SacI G_AGCT'C
Cuts at: 0 1096 1656
Size: 1096 560
SacII CC_GC'GG
Cuts at: 0 626 1656
Size: 626 1030
SapI GCTCTTCn'nnn_
Cuts at: 0 433 1345 1656
Size: 433 912 311
Fragments arranged by size:
912 433 311
ScaI AGT'ACT
Cuts at: 0 469 1656
Size: 469 1187
SexAI A'CCwGG_T
Cuts at: 0 791 1656
Size: 791 865
SfcI C'TryA_G
Cuts at: 0 484 927 1339 1470 1656
Size: 484 443 412 131 186
Fragments arranged by size:
484 443 412 186 131
SfoI GGC'GCC
Cuts at: 0 753 1416 1656
Size: 753 663 240
Fragments arranged by size:
753 663 240
SmaI CCC'GGG
Cuts at: 0 1297 1426 1656
Size: 1297 129 230
Fragments arranged by size:
1297 230 129
SmlI C'TyrA_G
Cuts at: 0 1089 1656
Size: 1089 567
StuI AGG'CCT
Cuts at: 0 685 1656
Size: 685 971
StyI C'CwwG_G
Cuts at: 0 98 638 1097 1599 1656
Size: 98 540 459 502 57
Fragments arranged by size:
540 502 459 98 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 903 1656
Size: 903 753
TatI w'GTAC_w
Cuts at: 0 467 488 1656
Size: 467 21 1168
Fragments arranged by size:
1168 467 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 618 1656
Size: 586 32 1038
Fragments arranged by size:
1038 586 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 789 1656
Size: 789 867
XhoI C'TCGA_G
Cuts at: 0 1089 1656
Size: 1089 567
XmaI C'CCGG_G
Cuts at: 0 1295 1424 1656
Size: 1295 129 232
Fragments arranged by size:
1295 232 129
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI AvaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PfoI PshAI PspXI PstI PvuII SacI SacII SapI
ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BpuEI BsaI BsaBI BsiEI BsiWI
BsmI BsrBI BsrGI BssSI BstBI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI
HincII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PpuMI PsiI PspOMI PsrI PvuI RsrII SalI SanDI
SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XmnI ZraI