(Circular) (Six-base) MAPSORT of: pFN21AB9955.seq Check: 5970 from: 1 to: 6316 pFN21AB9955 6316 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3427 3427 Size: 6316 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4126 278 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3659 1937 Size: 1722 4594 Acc65I G'GTAC_C Cuts at: 2085 2516 2867 3637 2085 Size: 431 351 770 4764 Fragments arranged by size: 4764 770 431 351 AclI AA'CG_TT Cuts at: 1563 4443 4816 1563 Size: 2880 373 3063 Fragments arranged by size: 3063 2880 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2660 2987 3546 4379 5763 6031 1632 Size: 1028 327 559 833 1384 268 1917 Fragments arranged by size: 1917 1384 1028 833 559 327 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6119 AflIII A'CryG_T Cuts at: 1203 3348 5215 6192 1203 Size: 2145 1867 977 1327 Fragments arranged by size: 2145 1867 1327 977 AgeI A'CCGG_T Cuts at: 5147 5147 Size: 6316 AhdI GACnn_n'nnGTC Cuts at: 2189 3352 5045 2189 Size: 1163 1693 3460 Fragments arranged by size: 3460 1693 1163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6316 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2734 1184 Size: 1550 4766 AlwNI CAG_nnn'CTG Cuts at: 4059 5631 4059 Size: 1572 4744 ApaI G_GGCC'C Cuts at: 2662 2662 Size: 6316 ApaLI G'TGCA_C Cuts at: 4373 5529 4373 Size: 1156 5160 ApoI r'AATT_y Cuts at: 1493 2327 2616 3625 3851 4003 6112 1493 Size: 834 289 1009 226 152 2109 1697 Fragments arranged by size: 2109 1697 1009 834 289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4870 160 Size: 872 3838 1606 Fragments arranged by size: 3838 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 AvaI C'yCGr_G Cuts at: 1870 1943 2655 2805 3308 3641 1870 Size: 73 712 150 503 333 4545 Fragments arranged by size: 4545 712 503 333 150 73 BamHI G'GATC_C Cuts at: 3646 4013 3646 Size: 367 5949 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 2516 2552 2867 3637 Size: 325 217 627 298 431 36 315 770 Cuts at: 3637 5092 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 770 627 431 325 315 298 217 36 BanII G_rGCy'C Cuts at: 1391 2081 2662 3015 3088 3498 3591 3635 1391 Size: 690 581 353 73 410 93 44 4072 Fragments arranged by size: 4072 690 581 410 353 93 73 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3258 3573 928 Size: 971 1359 315 3671 Fragments arranged by size: 3671 1359 971 315 BbvCI CC'TCA_GC Cuts at: 2112 2112 Size: 6316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4507 4541 1903 Size: 34 2570 34 3678 Fragments arranged by size: 3678 2570 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2633 4208 5418 2633 Size: 1575 1210 3531 Fragments arranged by size: 3531 1575 1210 BclI T'GATC_A Cuts at: 1548 2823 1548 Size: 1275 5041 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3311 4927 136 Size: 107 122 71 631 605 1639 1616 1525 Fragments arranged by size: 1639 1616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3172 6311 3172 Size: 3139 3177 BlpI GC'TnA_GC Cuts at: 2481 3733 2481 Size: 1252 5064 Bme1580I G_kGCm'C Cuts at: 1790 2662 4377 5533 1790 Size: 872 1715 1156 2573 Fragments arranged by size: 2573 1715 1156 872 BmgBI CAC'GTC Cuts at: 1318 3349 1318 Size: 2031 4285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2656 2843 3586 Size: 416 53 83 399 24 1239 187 743 Cuts at: 3586 4047 5005 442 Size: 461 958 1753 Fragments arranged by size: 1753 1239 958 743 461 416 399 187 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6316 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2276 2276 Size: 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2406 3452 4976 1239 Size: 1167 1046 1524 2579 Fragments arranged by size: 2579 1524 1167 1046 Bpu10I CC'TnA_GC Cuts at: 2112 2112 Size: 6316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2960 4441 5306 5604 2960 Size: 1481 865 298 3672 Fragments arranged by size: 3672 1481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2589 2720 4979 882 Size: 617 1090 131 2259 2219 Fragments arranged by size: 2259 2219 1090 617 131 BsaAI yAC'GTr Cuts at: 493 1204 4107 493 Size: 711 2903 2702 Fragments arranged by size: 2903 2702 711 BsaBI GATnn'nnATC Cuts at: 6310 6310 Size: 6316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4123 4505 5140 275 Size: 53 83 186 936 2590 382 635 1451 Fragments arranged by size: 2590 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4749 5147 5421 5568 5979 4749 Size: 398 274 147 411 5086 Fragments arranged by size: 5086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2586 2616 1178 Size: 30 165 30 1183 30 4878 Fragments arranged by size: 4878 1183 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2256 3023 1186 Size: 880 190 767 4479 Fragments arranged by size: 4479 880 767 190 BseYI C'CCAG_C Cuts at: 2123 2405 5519 6015 2123 Size: 282 3114 496 2424 Fragments arranged by size: 3114 2424 496 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5743 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3766 4527 5555 1540 Size: 275 186 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2081 3088 3498 4377 4462 5533 2081 Size: 1007 410 879 85 1071 2864 Fragments arranged by size: 2864 1071 1007 879 410 85 BsiWI C'GTAC_G Cuts at: 2234 2234 Size: 6316 BsmI GAATG_Cn' Cuts at: 3827 3920 3827 Size: 93 6223 BsmBI CGTCTCn'nnnn_ Cuts at: 2220 2220 Size: 6316 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2662 3015 3088 3498 3591 3635 Size: 399 291 581 353 73 410 93 44 Cuts at: 3635 4377 4462 5533 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 581 410 399 353 291 93 85 73 44 BspEI T'CCGG_A Cuts at: 5979 5979 Size: 6316 BspHI T'CATG_A Cuts at: 2955 3498 4205 5935 2955 Size: 543 707 1730 3336 Fragments arranged by size: 3336 1730 707 543 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 3427 3671 844 Size: 1858 725 244 3489 Fragments arranged by size: 3489 1858 725 244 BsrBI CCG'CTC Cuts at: 1394 1823 2373 4203 1394 Size: 429 550 1830 3507 Fragments arranged by size: 3507 1830 550 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2024 4811 4985 65 Size: 1183 776 2787 174 1396 Fragments arranged by size: 2787 1396 1183 776 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4960 5147 1700 Size: 110 3150 187 2869 Fragments arranged by size: 3150 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2686 96 Size: 2590 3726 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6316 BssSI C'ACGA_G Cuts at: 4376 5388 4376 Size: 1012 5304 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6316 BstBI TT'CG_AA Cuts at: 5120 5120 Size: 6316 BstEII G'GTnAC_C Cuts at: 1184 3382 1184 Size: 2198 4118 BstXI CCAn_nnnn'nTGG Cuts at: 2081 2081 Size: 6316 BstYI r'GATC_y Cuts at: 1972 3172 3646 4013 4398 4415 5867 5953 5965 Size: 1200 474 367 385 17 1452 86 12 Cuts at: 5965 6311 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1200 474 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4825 Fragments arranged by size: 4825 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5577 Fragments arranged by size: 5577 683 56 BtsI GCAGTG_nn' Cuts at: 3003 3505 3832 4626 4646 3003 Size: 502 327 794 20 4673 Fragments arranged by size: 4673 794 502 327 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6316 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3087 3122 529 Size: 35 2523 35 3723 Fragments arranged by size: 3723 2523 35 35 DraI TTT'AAA Cuts at: 3620 3620 Size: 6316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2981 5323 809 Size: 507 1665 2342 1802 Fragments arranged by size: 2342 1802 1665 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2467 3763 4652 Size: 54 1425 50 275 18 637 1296 889 Cuts at: 4652 6281 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1296 889 637 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3763 1537 Size: 275 1951 4090 Fragments arranged by size: 4090 1951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2215 2439 3076 3388 4246 1353 Size: 862 224 637 312 858 3423 Fragments arranged by size: 3423 862 858 637 312 224 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4903 5277 5423 387 Size: 1064 138 3314 374 146 1280 Fragments arranged by size: 3314 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2079 3086 3496 2079 Size: 1007 410 4899 Fragments arranged by size: 4899 1007 410 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2406 2660 2987 3452 3546 4379 4976 Size: 393 774 254 327 465 94 833 597 Cuts at: 4976 5763 6031 1239 Size: 787 268 1524 Fragments arranged by size: 1524 833 787 774 597 465 393 327 268 254 94 EcoO109I rG'GnC_Cy Cuts at: 2226 3520 2226 Size: 1294 5022 EcoRI G'AATT_C Cuts at: 3625 4003 3625 Size: 378 5938 FspI TGC'GCA Cuts at: 4822 4822 Size: 6316 HaeII r_GCGC'y Cuts at: 1678 5463 1678 Size: 3785 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2175 2207 2586 2618 3164 Size: 32 135 32 603 32 379 32 546 Cuts at: 3164 3196 3338 3370 4957 4989 5031 5063 1373 Size: 32 142 32 1587 32 42 32 2626 Fragments arranged by size: 2626 1587 603 546 379 142 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2428 3660 3906 669 Size: 988 281 490 1232 246 3079 Fragments arranged by size: 3079 1232 988 490 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6316 HpaI GTT'AAC Cuts at: 3906 3906 Size: 6316 KpnI G_GTAC'C Cuts at: 2089 2520 2871 3641 2089 Size: 431 351 770 4764 Fragments arranged by size: 4764 770 431 351 MfeI C'AATT_G Cuts at: 3915 3915 Size: 6316 MluI A'CGCG_T Cuts at: 6192 6192 Size: 6316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5430 5614 1305 Size: 4125 184 2007 Fragments arranged by size: 4125 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4494 Fragments arranged by size: 4494 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3194 4274 4633 4792 518 Size: 648 2028 1080 359 159 2042 Fragments arranged by size: 2042 2028 1080 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3732 4409 5557 5802 1606 Size: 2126 677 1148 245 2120 Fragments arranged by size: 2126 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4825 Fragments arranged by size: 4825 939 552 NdeI CA'TA_TG Cuts at: 387 3212 387 Size: 2825 3491 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6316 NotI GC'GGCC_GC Cuts at: 3763 3763 Size: 6316 NspI r_CATG'y Cuts at: 1132 1229 3674 5219 1132 Size: 97 2445 1545 2229 Fragments arranged by size: 2445 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6316 PciI A'CATG_T Cuts at: 5215 5215 Size: 6316 PflMI CCAn_nnn'nTGG Cuts at: 1108 3297 1108 Size: 2189 4127 PfoI T'CCnGG_A Cuts at: 3561 3561 Size: 6316 PmeI GTTT'AAAC Cuts at: 3620 3620 Size: 6316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2063 2095 4385 4417 5898 5930 2063 Size: 32 2290 32 1481 32 2449 Fragments arranged by size: 2449 2290 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2226 3520 2226 Size: 1294 5022 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6316 PsiI TTA'TAA Cuts at: 2613 3886 2613 Size: 1273 5043 PspOMI G'GGCC_C Cuts at: 2658 2658 Size: 6316 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3314 3346 3314 Size: 32 6284 PstI C_TGCA'G Cuts at: 830 2713 2820 3133 3668 830 Size: 1883 107 313 535 3478 Fragments arranged by size: 3478 1883 535 313 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6316 SacI G_AGCT'C Cuts at: 2081 3088 3498 2081 Size: 1007 410 4899 Fragments arranged by size: 4899 1007 410 SalI G'TCGA_C Cuts at: 1936 3658 1936 Size: 1722 4594 SapI GCTCTTCn'nnn_ Cuts at: 3076 3076 Size: 6316 SbfI CC_TGCA'GG Cuts at: 3668 3668 Size: 6316 ScaI AGT'ACT Cuts at: 3548 4564 6276 3548 Size: 1016 1712 3588 Fragments arranged by size: 3588 1712 1016 SexAI A'CCwGG_T Cuts at: 3601 3612 3601 Size: 11 6305 SfcI C'TryA_G Cuts at: 826 1045 2709 2816 3129 3451 3664 4799 5480 Size: 219 1664 107 313 322 213 1135 681 Cuts at: 5480 5671 826 Size: 191 1471 Fragments arranged by size: 1664 1471 1135 681 322 313 219 213 191 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6316 SmaI CCC'GGG Cuts at: 1872 2657 3643 1872 Size: 785 986 4545 Fragments arranged by size: 4545 986 785 SmlI C'TyrA_G Cuts at: 820 1017 1943 2805 2939 4420 5321 5583 820 Size: 197 926 862 134 1481 901 262 1553 Fragments arranged by size: 1553 1481 926 901 862 262 197 134 SnaBI TAC'GTA Cuts at: 493 4107 493 Size: 3614 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6316 SphI G_CATG'C Cuts at: 1132 3674 1132 Size: 2542 3774 SspI AAT'ATT Cuts at: 5 52 2337 4240 5 Size: 47 2285 1903 2081 Fragments arranged by size: 2285 2081 1903 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6316 StyI C'CwwG_G Cuts at: 513 1065 2004 2179 2756 3755 513 Size: 552 939 175 577 999 3074 Fragments arranged by size: 3074 999 939 577 552 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4343 4360 4513 4698 5143 325 Size: 1255 2763 17 153 185 445 1498 Fragments arranged by size: 2763 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2686 3261 3546 Size: 274 80 33 51 1443 709 575 285 Cuts at: 3546 4562 6274 96 Size: 1016 1712 138 Fragments arranged by size: 1712 1443 1016 709 575 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2238 2270 2553 2585 6276 6308 2238 Size: 32 283 32 3691 32 2246 Fragments arranged by size: 3691 2246 283 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2600 1316 Size: 1284 5032 XbaI T'CTAG_A Cuts at: 3652 3652 Size: 6316 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2413 3599 2413 Size: 1186 5130 XhoI C'TCGA_G Cuts at: 1943 2805 1943 Size: 862 5454 XmaI C'CCGG_G Cuts at: 1870 2655 3641 1870 Size: 785 986 4545 Fragments arranged by size: 4545 986 785 XmnI GAAnn'nnTTC Cuts at: 2328 2673 4445 2328 Size: 345 1772 4199 Fragments arranged by size: 4199 1772 345 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4124 276 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI