Restriction map for
pFN21AB9919
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AspHI 394 GAGCAC G[AT]GC[AT]^C
BclI 500 TGATCA T^GATCA
BsaWI 660 TCCGGT [AT]^CCGG[AT]
BsgI 139 CTGCAC GTGCAG(16/14)
BsiHKAI 394 GAGCAC G[AT]GC[AT]^C
BspMI 516 ACCTGC ACCTGC(4/8)
BstAPI 578 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 579 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 822 CCATGG C^C[GA][CT]GG
FbaI 500 TGATCA T^GATCA
HaeII 198 GGCGCT [GA]GCGC^[CT]
HgaI 158 GACGC GACGC(5/10)
MaeIII 764 GTGAC ^GT[ACGT]AC
MboII 837 GAAGA GAAGA(8/7)
MseI 887 TTAA T^TAA
NcoI 822 CCATGG C^CATGG
NspI 716 GCATGT [GA]CATG^[CT]
PflMI 568 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 569 CAGCTG CAG^CTG
SexAI 281 ACCAGGT A^CC[AT]GGT
SmlI 736 CTTGAG C^T[CT][GA]AG
TfiI 751 GATTC G^A[AT]TC
Tru9I 887 TTAA T^TAA
Tsp45I 764 GTGAC ^GT[GC]AC
Van91I 568 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 853 GTCTAC GT^[AC][GT]AC
865 GTCTAC
AccII 176 CGCG CG^CG
535 CGCG
AcsI 267 GAATTC [GA]^AATT[CT]
294 GAATTC
AlwNI 209 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
569 CAGCTGCTG
ApoI 267 GAATTC [GA]^AATT[CT]
294 GAATTC
AvaII 7 GGACC G^G[AT]CC
40 GGACC
BcnI 37 CCGGG CC^[GC]GG
43 CCGGG
BmyI 204 GGGCAC G[AGT]GC[ACT]^C
394 GAGCAC
BseRI 510 CTCCTC GAGGAG(10/8)
262 GAGGAG
BsmAI 384 GAGAC GTCTC(1/5)
811 GTCTC
Bsp1286I 204 GGGCAC G[AGT]GC[ACT]^C
394 GAGCAC
BsrBI 100 GAGCGG CCGCTC(-3/-3)
170 GAGCGG
BsrI 387 ACTGG ACTGG(1/-1)
881 ACTGG
BstUI 176 CGCG CG^CG
535 CGCG
DpnII 447 GATC ^GATC
501 GATC
EaeI 34 TGGCCG [CT]^GGCC[GA]
440 CGGCCA
EcoRI 267 GAATTC G^AATTC
294 GAATTC
HphI 381 GGTGA GGTGA(8/7)
514 TCACC
MboI 447 GATC ^GATC
501 GATC
MslI 375 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
823 CATGGCTGTG
MspA1I 115 CAGCGG C[AC]G^C[GT]G
569 CAGCTG
MvnI 176 CGCG CG^CG
535 CGCG
NciI 37 CCGGG CC^[GC]GG
43 CCGGG
NdeII 447 GATC ^GATC
501 GATC
PleI 766 GACTC GAGTC(4/5)
851 GAGTC
Sau3AI 447 GATC ^GATC
501 GATC
StuI 85 AGGCCT AGG^CCT
844 AGGCCT
Tsp509I 268 AATT ^AATT
295 AATT
XcmI 306 CCACTTCTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
759 CCAGCGTGACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MnlI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI StyI TseI TspRI