(Circular) (Six-base) MAPSORT of: pFN21AB6994.seq Check: 4079 from: 1 to: 5464 pFN21AB6994 5464 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2053 Size: 5464 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2325 3527 4911 5179 1632 Size: 693 1202 1384 268 1917 Fragments arranged by size: 1917 1384 1202 693 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5464 AlwNI CAG_nnn'CTG Cuts at: 2614 3207 4779 2614 Size: 593 1572 3299 Fragments arranged by size: 3299 1572 593 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2285 2398 2617 2715 2773 2999 3151 5260 Size: 792 113 219 98 58 226 152 2109 Cuts at: 5260 1493 Size: 1697 Fragments arranged by size: 2109 1697 792 226 219 152 113 98 58 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2789 1870 Size: 73 846 4545 Fragments arranged by size: 4545 846 73 BamHI G'GATC_C Cuts at: 2548 2794 3161 2548 Size: 246 367 4851 Fragments arranged by size: 4851 367 246 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2785 4240 618 Size: 325 217 627 998 1455 1842 Fragments arranged by size: 1842 1455 998 627 325 217 BanII G_rGCy'C Cuts at: 1391 2783 1391 Size: 1392 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2499 928 Size: 971 600 3893 Fragments arranged by size: 3893 971 600 BbvCI CC'TCA_GC Cuts at: 2240 2240 Size: 5464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 2314 1548 Size: 766 4698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4075 136 Size: 107 122 71 631 605 2403 1525 Fragments arranged by size: 2403 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2562 5459 2562 Size: 2897 2567 BlpI GC'TnA_GC Cuts at: 2102 2881 2102 Size: 779 4685 Bme1580I G_kGCm'C Cuts at: 1790 3525 4681 1790 Size: 1735 1156 2573 Fragments arranged by size: 2573 1735 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3195 4153 442 Size: 416 53 83 399 24 1778 958 1753 Fragments arranged by size: 1778 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2178 2246 2428 4124 1239 Size: 939 68 182 1696 2579 Fragments arranged by size: 2579 1696 939 182 68 Bpu10I CC'TnA_GC Cuts at: 2240 2240 Size: 5464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3589 4454 4752 3589 Size: 865 298 4301 Fragments arranged by size: 4301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2402 4127 882 Size: 617 903 1725 2219 Fragments arranged by size: 2219 1725 903 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2662 3897 4295 4569 4716 5127 2662 Size: 1235 398 274 147 411 2999 Fragments arranged by size: 2999 1235 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 4533 BseYI C'CCAG_C Cuts at: 2028 4667 5163 2028 Size: 2639 496 2329 Fragments arranged by size: 2639 2329 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4891 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3525 3610 4681 3525 Size: 85 1071 4308 Fragments arranged by size: 4308 1071 85 BsmI GAATG_Cn' Cuts at: 2536 2761 2975 3068 2536 Size: 225 214 93 4932 Fragments arranged by size: 4932 225 214 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2783 3525 3610 4681 1391 Size: 399 993 742 85 1071 2174 Fragments arranged by size: 2174 1071 993 742 399 85 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 2317 3353 5083 2317 Size: 1036 1730 2698 Fragments arranged by size: 2698 1730 1036 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 2244 2819 844 Size: 1209 191 575 3489 Fragments arranged by size: 3489 1209 575 191 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3959 4133 65 Size: 1183 2711 174 1396 Fragments arranged by size: 2711 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 4108 4295 1700 Size: 110 287 2011 187 2869 Fragments arranged by size: 2869 2011 287 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 2117 1925 Size: 192 5272 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstXI CCAn_nnnn'nTGG Cuts at: 2350 2350 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2548 2562 2794 3161 3546 3563 5015 5101 Size: 576 14 232 367 385 17 1452 86 Cuts at: 5101 5113 5459 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 576 385 367 346 232 86 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2049 2184 513 Size: 552 984 135 3793 Fragments arranged by size: 3793 984 552 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2099 2730 2980 3774 3794 2099 Size: 631 250 794 20 3769 Fragments arranged by size: 3769 794 631 250 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2169 2187 Size: 54 1425 50 275 18 222 117 18 Cuts at: 2187 2911 3800 5429 8 Size: 724 889 1629 43 Fragments arranged by size: 1629 1425 889 724 275 222 117 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2301 3394 1353 Size: 948 1093 3423 Fragments arranged by size: 3423 1093 948 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2178 2246 2325 2428 3527 4124 4911 Size: 393 546 68 79 103 1099 597 787 Cuts at: 4911 5179 1239 Size: 268 1524 Fragments arranged by size: 1524 1099 787 597 546 393 268 103 79 68 EcoO109I rG'GnC_Cy Cuts at: 2611 2611 Size: 5464 EcoRI G'AATT_C Cuts at: 2715 2773 3151 2715 Size: 58 378 5028 Fragments arranged by size: 5028 378 58 EcoRV GAT'ATC Cuts at: 2326 2326 Size: 5464 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 4611 1678 Size: 2933 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2734 2766 4105 4137 4179 Size: 32 135 32 1162 32 1339 32 42 Cuts at: 4179 4211 1373 Size: 32 2626 Fragments arranged by size: 2626 1339 1162 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 2537 748 Size: 1789 3675 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 2189 10 Size: 54 1425 343 339 18 3285 Fragments arranged by size: 3285 1425 343 339 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 3422 3781 3940 518 Size: 648 2256 359 159 2042 Fragments arranged by size: 2256 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2072 2880 3557 4705 4950 1606 Size: 445 21 808 677 1148 245 2120 Fragments arranged by size: 2120 1148 808 677 445 245 21 NaeI GCC'GGC Cuts at: 2099 2099 Size: 5464 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4912 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NgoMIV G'CCGG_C Cuts at: 2097 2097 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2110 2260 2584 2822 4367 1132 Size: 97 881 150 324 238 1545 2229 Fragments arranged by size: 2229 1545 881 324 238 150 97 PasI CC'CwG_GG Cuts at: 1348 2015 1348 Size: 667 4797 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PfoI T'CCnGG_A Cuts at: 2151 2151 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 2673 3034 2673 Size: 361 5103 PstI C_TGCA'G Cuts at: 830 2094 2816 830 Size: 1264 722 3478 Fragments arranged by size: 3478 1264 722 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 SacII CC_GC'GG Cuts at: 2052 2052 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SexAI A'CCwGG_T Cuts at: 2508 2508 Size: 5464 SfcI C'TryA_G Cuts at: 826 1045 2090 2812 3947 4628 4819 826 Size: 219 1045 722 1135 681 191 1471 Fragments arranged by size: 1471 1135 1045 722 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2791 1872 Size: 919 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3568 4469 4731 820 Size: 197 926 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 2357 1517 Size: 840 4624 StyI C'CwwG_G Cuts at: 513 1065 2903 513 Size: 552 1838 3074 Fragments arranged by size: 3074 1838 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3491 3508 3661 3846 4291 325 Size: 1255 1911 17 153 185 445 1498 Fragments arranged by size: 1911 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3710 5422 96 Size: 274 80 33 51 1443 1733 1712 138 Fragments arranged by size: 1733 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2281 2569 2800 2281 Size: 288 231 4945 Fragments arranged by size: 4945 288 231 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2789 1870 Size: 919 4545 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI