Restriction map for
pFN21AB6994
(Linear) (Six-base) MAPSORT of: KIBB6994.seq Check: 7480 from: 1 to: 759
KIBB6994.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 759
Size: 48 711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 759
Size: 320 439
AlwNI CAG_nnn'CTG
Cuts at: 0 609 759
Size: 609 150
ApoI r'AATT_y
Cuts at: 0 280 393 612 710 759
Size: 280 113 219 98 49
Fragments arranged by size:
280 219 113 98 49
BamHI G'GATC_C
Cuts at: 0 543 759
Size: 543 216
BbsI GAAGACnn'nnnn_
Cuts at: 0 494 759
Size: 494 265
BbvCI CC'TCA_GC
Cuts at: 0 235 759
Size: 235 524
BclI T'GATC_A
Cuts at: 0 309 759
Size: 309 450
BglII A'GATC_T
Cuts at: 0 557 759
Size: 557 202
BlpI GC'TnA_GC
Cuts at: 0 97 759
Size: 97 662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 241 423 759
Size: 173 68 182 336
Fragments arranged by size:
336 182 173 68
Bpu10I CC'TnA_GC
Cuts at: 0 235 759
Size: 235 524
BsaI GGTCTCn'nnnn_
Cuts at: 0 397 759
Size: 397 362
BsaWI w'CCGG_w
Cuts at: 0 657 759
Size: 657 102
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 759
Size: 112 647
BseYI C'CCAG_C
Cuts at: 0 23 759
Size: 23 736
BsmI GAATG_Cn'
Cuts at: 0 531 756 759
Size: 531 225 3
Fragments arranged by size:
531 225 3
BspHI T'CATG_A
Cuts at: 0 312 759
Size: 312 447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 239 759
Size: 48 191 520
Fragments arranged by size:
520 191 48
BsrFI r'CCGG_y
Cuts at: 0 92 759
Size: 92 667
BssHII G'CGCG_C
Cuts at: 0 112 759
Size: 112 647
BstXI CCAn_nnnn'nTGG
Cuts at: 0 345 759
Size: 345 414
BstYI r'GATC_y
Cuts at: 0 543 557 759
Size: 543 14 202
Fragments arranged by size:
543 202 14
BtgI C'CryG_G
Cuts at: 0 44 179 759
Size: 44 135 580
Fragments arranged by size:
580 135 44
BtsI GCAGTG_nn'
Cuts at: 0 94 725 759
Size: 94 631 34
Fragments arranged by size:
631 94 34
EaeI y'GGCC_r
Cuts at: 0 47 164 182 759
Size: 47 117 18 577
Fragments arranged by size:
577 117 47 18
EarI CTCTTCn'nnn_
Cuts at: 0 296 759
Size: 296 463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 241 320 423 759
Size: 173 68 79 103 336
Fragments arranged by size:
336 173 103 79 68
EcoO109I rG'GnC_Cy
Cuts at: 0 606 759
Size: 606 153
EcoRI G'AATT_C
Cuts at: 0 710 759
Size: 710 49
EcoRV GAT'ATC
Cuts at: 0 321 759
Size: 321 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 729 759
Size: 729 30
HindIII A'AGCT_T
Cuts at: 0 532 759
Size: 532 227
MscI TGG'CCA
Cuts at: 0 166 184 759
Size: 166 18 575
Fragments arranged by size:
575 166 18
MspA1I CmG'CkG
Cuts at: 0 46 67 759
Size: 46 21 692
Fragments arranged by size:
692 46 21
NaeI GCC'GGC
Cuts at: 0 94 759
Size: 94 665
NgoMIV G'CCGG_C
Cuts at: 0 92 759
Size: 92 667
NspI r_CATG'y
Cuts at: 0 105 255 579 759
Size: 105 150 324 180
Fragments arranged by size:
324 180 150 105
PasI CC'CwG_GG
Cuts at: 0 10 759
Size: 10 749
PfoI T'CCnGG_A
Cuts at: 0 146 759
Size: 146 613
PsiI TTA'TAA
Cuts at: 0 668 759
Size: 668 91
PstI C_TGCA'G
Cuts at: 0 89 759
Size: 89 670
SacII CC_GC'GG
Cuts at: 0 47 759
Size: 47 712
SexAI A'CCwGG_T
Cuts at: 0 503 759
Size: 503 256
SfcI C'TryA_G
Cuts at: 0 85 759
Size: 85 674
StuI AGG'CCT
Cuts at: 0 352 759
Size: 352 407
XbaI T'CTAG_A
Cuts at: 0 276 564 759
Size: 276 288 195
Fragments arranged by size:
288 276 195
Enzymes that do cut:
AarI AcuI AlwNI ApoI BamHI BbsI BbvCI BclI
BglII BlpI BpmI Bpu10I BsaI BsaWI BseRI BseYI
BsmI BspHI BspMI BsrFI BssHII BstXI BstYI BtgI
BtsI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV Hin4I
HindIII MscI MspA1I NaeI NgoMIV NspI PasI PfoI
PsiI PstI SacII SexAI SfcI StuI XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BcgI
BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MslI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI