Restriction map for pFN21AB6989
 (Circular) (Six-base) MAPSORT of: pFN21AB6989.seq  Check: 4030  from: 1  to: 5362



pFN21AB6989  5362 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:42  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3172     278 
   Size:         53      83     186     936    1636    2468

  Fragments arranged by size: 

                2468    1636     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2705    1937 
   Size:        768    4594
 
Acc65I G'GTAC_C

Cuts at:   2683    2683 
   Size:       5362
 
AclI AA'CG_TT

Cuts at:   1563    3489    3862    1563 
   Size:       1926     373    3063

  Fragments arranged by size: 

                3063    1926     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3425    4809    5077    1632 
   Size:       1793    1384     268    1917

  Fragments arranged by size: 

                1917    1793    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5165
 
AflIII A'CryG_T

Cuts at:   1203    4261    5238    1203 
   Size:       3058     977    1327

  Fragments arranged by size: 

                3058    1327     977
 
AgeI A'CCGG_T

Cuts at:   4193    4193 
   Size:       5362
 
AhdI GACnn_n'nnGTC

Cuts at:   2164    4091    2164 
   Size:       1927    3435
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5362
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2657    1184 
   Size:       1473    3889
 
AlwNI CAG_nnn'CTG

Cuts at:   2213    3105    4677    2213 
   Size:        892    1572    2898

  Fragments arranged by size: 

                2898    1572     892
 
ApaLI G'TGCA_C

Cuts at:   2193    3419    4575    2193 
   Size:       1226    1156    2980

  Fragments arranged by size: 

                2980    1226    1156
 
ApoI r'AATT_y

Cuts at:   1493    2648    2671    2897    3049    5158    1493 
   Size:       1155      23     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1155     226     152      23
 
AseI AT'TA_AT

Cuts at:    160    1032    3916     160 
   Size:        872    2884    1606

  Fragments arranged by size: 

                2884    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5362
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2040    2118    2507    2687    1870 
   Size:         73      97      78     389     180    4545

  Fragments arranged by size: 

                4545     389     180      97      78      73
 
BamHI G'GATC_C

Cuts at:   2692    3059    2692 
   Size:        367    4995
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2683    4138     618 
   Size:        325     217     627     896    1455    1842

  Fragments arranged by size: 

                1842    1455     896     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2259    2681    1391 
   Size:        868     422    4072

  Fragments arranged by size: 

                4072     868     422
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4391
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3553    3587    1903 
   Size:         34      50      34    1532      34    3678

  Fragments arranged by size: 

                3678    1532      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3254    4464    3254 
   Size:       1210    4152
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5362
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2237    2562    3973 
   Size:        107     122      71     631     605     565     325    1411

Cuts at:   3973     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1411     631     605     565     325     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5357    5357 
   Size:       5362
 
BlpI GC'TnA_GC

Cuts at:   2779    2779 
   Size:       5362
 
Bme1580I G_kGCm'C

Cuts at:   1790    2197    3423    4579    1790 
   Size:        407    1226    1156    2573

  Fragments arranged by size: 

                2573    1226    1156     407
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5362
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3093    4051     442 
   Size:        416      53      83     399      24    1676     958    1753

  Fragments arranged by size: 

                1753    1676     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5362
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2273    2294    4022    1239 
   Size:       1034      21    1728    2579

  Fragments arranged by size: 

                2579    1728    1034      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2551    3487    4352    4650    2551 
   Size:        936     865     298    3263

  Fragments arranged by size: 

                3263     936     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4025     882 
   Size:        617    2526    2219

  Fragments arranged by size: 

                2526    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3153     493 
   Size:        711    1949    2702

  Fragments arranged by size: 

                2702    1949     711
 
BsaBI GATnn'nnATC

Cuts at:   5356    5356 
   Size:       5362
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3169    3551    4186     275 
   Size:         53      83     186     936    1636     382     635    1451

  Fragments arranged by size: 

                1636    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3795    4193    4467    4614    5025    3795 
   Size:        398     274     147     411    4132

  Fragments arranged by size: 

                4132     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2379    2409    1178 
   Size:         30     165      30     976      30    4131

  Fragments arranged by size: 

                4131     976     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2525    1186 
   Size:       1339    4023
 
BseYI C'CCAG_C

Cuts at:   2370    4565    5061    2370 
   Size:       2195     496    2671

  Fragments arranged by size: 

                2671    2195     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2159    2334    2536    1163 
   Size:        573     423     175     202    3989

  Fragments arranged by size: 

                3989     573     423     202     175
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2812    3573    4601    1540 
   Size:        275     186     811     761    1028    2301

  Fragments arranged by size: 

                2301    1028     811     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2023    2197    2259    3423    3508    4579    2023 
   Size:        174      62    1164      85    1071    2806

  Fragments arranged by size: 

                2806    1164    1071     174      85      62
 
BsmI GAATG_Cn'

Cuts at:   2421    2873    2966    2421 
   Size:        452      93    4817

  Fragments arranged by size: 

                4817     452      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2023    2197    2259    2681    3423    3508    4579 
   Size:        399     233     174      62     422     742      85    1071

Cuts at:   4579    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     422     399     233     174      85
                  62
 
BspEI T'CCGG_A

Cuts at:   5025    5025 
   Size:       5362
 
BspHI T'CATG_A

Cuts at:   3251    4981    3251 
   Size:       1730    3632
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2717     844 
   Size:       1873    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3249    1394 
   Size:        429    1426    3507

  Fragments arranged by size: 

                3507    1426     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2641    3857    4031      65 
   Size:       1183    1393    1216     174    1396

  Fragments arranged by size: 

                1396    1393    1216    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2606    4006    4193    1700 
   Size:        110     796    1400     187    2869

  Fragments arranged by size: 

                2869    1400     796     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5362
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5362
 
BssSI C'ACGA_G

Cuts at:   2177    3422    4434    2177 
   Size:       1245    1012    3105

  Fragments arranged by size: 

                3105    1245    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5362
 
BstBI TT'CG_AA

Cuts at:   4166    4166 
   Size:       5362
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5362
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2074    2074 
   Size:       5362
 
BstYI r'GATC_y

Cuts at:   1972    2692    3059    3444    3461    4913    4999    5011    5357 
   Size:        720     367     385      17    1452      86      12     346

Cuts at:   5357    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     720     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2226    2260     513 
   Size:        552    1161      34    3615

  Fragments arranged by size: 

                3615    1161     552      34
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4623

  Fragments arranged by size: 

                4623     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2085    2878    3672    3692    2085 
   Size:        793     794      20    3755

  Fragments arranged by size: 

                3755     794     793      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5362
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2274    2309     529 
   Size:         35    1710      35    3582

  Fragments arranged by size: 

                3582    1710      35      35
 
DraI TTT'AAA

Cuts at:   2666    2666 
   Size:       5362
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2621    4369     809 
   Size:        507    1305    1748    1802

  Fragments arranged by size: 

                1802    1748    1305     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2229    2238    2547 
   Size:         54    1425      50     275      18     399       9     309

Cuts at:   2547    2809    3698    5327       8 
   Size:        262     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     399     309     275     262      54
                  50      43      18       9
 
EagI C'GGCC_G

Cuts at:   1537    1812    2809    1537 
   Size:        275     997    4090

  Fragments arranged by size: 

                4090     997     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3292    1353 
   Size:       1939    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3949    4323    4469     387 
   Size:       1064     138    2360     374     146    1280

  Fragments arranged by size: 

                2360    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2257    2257 
   Size:       5362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2273    2294    3425    4022    4809    5077    1239 
   Size:        393     641      21    1131     597     787     268    1524

  Fragments arranged by size: 

                1524    1131     787     641     597     393     268      21
            
 
EcoRI G'AATT_C

Cuts at:   2671    3049    2671 
   Size:        378    4984
 
FspI TGC'GCA

Cuts at:   3868    3868 
   Size:       5362
 
HaeII r_GCGC'y

Cuts at:   1678    2524    4509    1678 
   Size:        846    1985    2531

  Fragments arranged by size: 

                2531    1985     846
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2150    2182    2330    2362    4003 
   Size:         32     135      32     578      32     148      32    1641

Cuts at:   4003    4035    4077    4109    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1641     578     148     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2706    2952     669 
   Size:        988     281     768     246    3079

  Fragments arranged by size: 

                3079     988     768     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5362
 
HpaI GTT'AAC

Cuts at:   2952    2952 
   Size:       5362
 
KpnI G_GTAC'C

Cuts at:   2687    2687 
   Size:       5362
 
MfeI C'AATT_G

Cuts at:   2961    2961 
   Size:       5362
 
MluI A'CGCG_T

Cuts at:   5238    5238 
   Size:       5362
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4476    4660    1305 
   Size:       3171     184    2007

  Fragments arranged by size: 

                3171    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2549      10 
   Size:         54    1425     343     717    2823

  Fragments arranged by size: 

                2823    1425     717     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2072    2078    3320    3679    3838     518 
   Size:        648     906       6    1242     359     159    2042

  Fragments arranged by size: 

                2042    1242     906     648     359     159       6
 
MspA1I CmG'CkG

Cuts at:   1606    2173    2210    2251    2603    2778    3455    4603    4848 
   Size:        567      37      41     352     175     677    1148     245

Cuts at:   4848    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     567     352     245     175      41
                  37
 
NcoI C'CATG_G

Cuts at:    513    1065    2260     513 
   Size:        552    1195    3615

  Fragments arranged by size: 

                3615    1195     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5362
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5362
 
NotI GC'GGCC_GC

Cuts at:   2809    2809 
   Size:       5362
 
NspI r_CATG'y

Cuts at:   1132    1229    2503    2720    4265    1132 
   Size:         97    1274     217    1545    2229

  Fragments arranged by size: 

                2229    1545    1274     217      97
 
PasI CC'CwG_GG

Cuts at:   1348    2407    1348 
   Size:       1059    4303
 
PciI A'CATG_T

Cuts at:   4261    4261 
   Size:       5362
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2227    1108 
   Size:       1119    4243
 
PmeI GTTT'AAAC

Cuts at:   2666    2666 
   Size:       5362
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3431    3463    4944    4976    3431 
   Size:         32    1481      32    3817

  Fragments arranged by size: 

                3817    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5362
 
PsiI TTA'TAA

Cuts at:   2932    2932 
   Size:       5362
 
PstI C_TGCA'G

Cuts at:    830    2131    2714     830 
   Size:       1301     583    3478

  Fragments arranged by size: 

                3478    1301     583
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5362
 
PvuII CAG'CTG

Cuts at:   2210    2251    2603    2210 
   Size:         41     352    4969

  Fragments arranged by size: 

                4969     352      41
 
SacI G_AGCT'C

Cuts at:   2259    2259 
   Size:       5362
 
SalI G'TCGA_C

Cuts at:   1936    2704    1936 
   Size:        768    4594
 
SbfI CC_TGCA'GG

Cuts at:   2714    2714 
   Size:       5362
 
ScaI AGT'ACT

Cuts at:   3610    5322    3610 
   Size:       1712    3650
 
SexAI A'CCwGG_T

Cuts at:   2586    2586 
   Size:       5362
 
SfcI C'TryA_G

Cuts at:    826    1045    2127    2710    3845    4526    4717     826 
   Size:        219    1082     583    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1082     681     583     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2237    2237 
   Size:       5362
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5362
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5362
 
SmaI CCC'GGG

Cuts at:   1872    2042    2689    1872 
   Size:        170     647    4545

  Fragments arranged by size: 

                4545     647     170
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2566    3466    4367    4629     820 
   Size:        197     926     623     900     901     262    1553

  Fragments arranged by size: 

                1553     926     901     900     623     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3153     493 
   Size:       2660    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5362
 
SphI G_CATG'C

Cuts at:   1132    2720    1132 
   Size:       1588    3774
 
SspI AAT'ATT

Cuts at:      5      52    3286       5 
   Size:         47    3234    2081

  Fragments arranged by size: 

                3234    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5362
 
StyI C'CwwG_G

Cuts at:    513    1065    2260    2801     513 
   Size:        552    1195     541    3074

  Fragments arranged by size: 

                3074    1195     552     541
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3389    3406    3559    3744    4189     325 
   Size:       1255    1809      17     153     185     445    1498

  Fragments arranged by size: 

                1809    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3608    5320      96 
   Size:        274      80      33      51    1443    1631    1712     138

  Fragments arranged by size: 

                1712    1631    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5322    5354    5322 
   Size:         32    5330
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5362
 
XbaI T'CTAG_A

Cuts at:   2698    2698 
   Size:       5362
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2077    2077 
   Size:       5362
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5362
 
XmaI C'CCGG_G

Cuts at:   1870    2040    2687    1870 
   Size:        170     647    4545

  Fragments arranged by size: 

                4545     647     170
 
XmnI GAAnn'nnTTC

Cuts at:   3491    3491 
   Size:       5362
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3170     276 
   Size:         53      83     186     936    1636    2468

  Fragments arranged by size: 

                2468    1636     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SacI     SalI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I
   DraIII    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    RsrII    SacII    SanDI     SapI
     SfoI     SrfI     SwaI
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