(Circular) (Six-base) MAPSORT of: pFN21AB6989.seq Check: 4030 from: 1 to: 5362 pFN21AB6989 5362 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3172 278 Size: 53 83 186 936 1636 2468 Fragments arranged by size: 2468 1636 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2705 1937 Size: 768 4594 Acc65I G'GTAC_C Cuts at: 2683 2683 Size: 5362 AclI AA'CG_TT Cuts at: 1563 3489 3862 1563 Size: 1926 373 3063 Fragments arranged by size: 3063 1926 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3425 4809 5077 1632 Size: 1793 1384 268 1917 Fragments arranged by size: 1917 1793 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5165 AflIII A'CryG_T Cuts at: 1203 4261 5238 1203 Size: 3058 977 1327 Fragments arranged by size: 3058 1327 977 AgeI A'CCGG_T Cuts at: 4193 4193 Size: 5362 AhdI GACnn_n'nnGTC Cuts at: 2164 4091 2164 Size: 1927 3435 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5362 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2657 1184 Size: 1473 3889 AlwNI CAG_nnn'CTG Cuts at: 2213 3105 4677 2213 Size: 892 1572 2898 Fragments arranged by size: 2898 1572 892 ApaLI G'TGCA_C Cuts at: 2193 3419 4575 2193 Size: 1226 1156 2980 Fragments arranged by size: 2980 1226 1156 ApoI r'AATT_y Cuts at: 1493 2648 2671 2897 3049 5158 1493 Size: 1155 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1155 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 3916 160 Size: 872 2884 1606 Fragments arranged by size: 2884 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5362 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2118 2507 2687 1870 Size: 73 97 78 389 180 4545 Fragments arranged by size: 4545 389 180 97 78 73 BamHI G'GATC_C Cuts at: 2692 3059 2692 Size: 367 4995 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2683 4138 618 Size: 325 217 627 896 1455 1842 Fragments arranged by size: 1842 1455 896 627 325 217 BanII G_rGCy'C Cuts at: 1391 2259 2681 1391 Size: 868 422 4072 Fragments arranged by size: 4072 868 422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3553 3587 1903 Size: 34 50 34 1532 34 3678 Fragments arranged by size: 3678 1532 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3254 4464 3254 Size: 1210 4152 BclI T'GATC_A Cuts at: 1548 1548 Size: 5362 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2237 2562 3973 Size: 107 122 71 631 605 565 325 1411 Cuts at: 3973 136 Size: 1525 Fragments arranged by size: 1525 1411 631 605 565 325 122 107 71 BglII A'GATC_T Cuts at: 5357 5357 Size: 5362 BlpI GC'TnA_GC Cuts at: 2779 2779 Size: 5362 Bme1580I G_kGCm'C Cuts at: 1790 2197 3423 4579 1790 Size: 407 1226 1156 2573 Fragments arranged by size: 2573 1226 1156 407 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5362 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3093 4051 442 Size: 416 53 83 399 24 1676 958 1753 Fragments arranged by size: 1753 1676 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5362 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2273 2294 4022 1239 Size: 1034 21 1728 2579 Fragments arranged by size: 2579 1728 1034 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2551 3487 4352 4650 2551 Size: 936 865 298 3263 Fragments arranged by size: 3263 936 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4025 882 Size: 617 2526 2219 Fragments arranged by size: 2526 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3153 493 Size: 711 1949 2702 Fragments arranged by size: 2702 1949 711 BsaBI GATnn'nnATC Cuts at: 5356 5356 Size: 5362 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3169 3551 4186 275 Size: 53 83 186 936 1636 382 635 1451 Fragments arranged by size: 1636 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3795 4193 4467 4614 5025 3795 Size: 398 274 147 411 4132 Fragments arranged by size: 4132 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2379 2409 1178 Size: 30 165 30 976 30 4131 Fragments arranged by size: 4131 976 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 1186 Size: 1339 4023 BseYI C'CCAG_C Cuts at: 2370 4565 5061 2370 Size: 2195 496 2671 Fragments arranged by size: 2671 2195 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 2334 2536 1163 Size: 573 423 175 202 3989 Fragments arranged by size: 3989 573 423 202 175 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2812 3573 4601 1540 Size: 275 186 811 761 1028 2301 Fragments arranged by size: 2301 1028 811 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2023 2197 2259 3423 3508 4579 2023 Size: 174 62 1164 85 1071 2806 Fragments arranged by size: 2806 1164 1071 174 85 62 BsmI GAATG_Cn' Cuts at: 2421 2873 2966 2421 Size: 452 93 4817 Fragments arranged by size: 4817 452 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2197 2259 2681 3423 3508 4579 Size: 399 233 174 62 422 742 85 1071 Cuts at: 4579 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 422 399 233 174 85 62 BspEI T'CCGG_A Cuts at: 5025 5025 Size: 5362 BspHI T'CATG_A Cuts at: 3251 4981 3251 Size: 1730 3632 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2717 844 Size: 1873 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3249 1394 Size: 429 1426 3507 Fragments arranged by size: 3507 1426 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2641 3857 4031 65 Size: 1183 1393 1216 174 1396 Fragments arranged by size: 1396 1393 1216 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2606 4006 4193 1700 Size: 110 796 1400 187 2869 Fragments arranged by size: 2869 1400 796 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5362 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5362 BssSI C'ACGA_G Cuts at: 2177 3422 4434 2177 Size: 1245 1012 3105 Fragments arranged by size: 3105 1245 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5362 BstBI TT'CG_AA Cuts at: 4166 4166 Size: 5362 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5362 BstXI CCAn_nnnn'nTGG Cuts at: 2074 2074 Size: 5362 BstYI r'GATC_y Cuts at: 1972 2692 3059 3444 3461 4913 4999 5011 5357 Size: 720 367 385 17 1452 86 12 346 Cuts at: 5357 1972 Size: 1977 Fragments arranged by size: 1977 1452 720 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2226 2260 513 Size: 552 1161 34 3615 Fragments arranged by size: 3615 1161 552 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4623 Fragments arranged by size: 4623 683 56 BtsI GCAGTG_nn' Cuts at: 2085 2878 3672 3692 2085 Size: 793 794 20 3755 Fragments arranged by size: 3755 794 793 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5362 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2274 2309 529 Size: 35 1710 35 3582 Fragments arranged by size: 3582 1710 35 35 DraI TTT'AAA Cuts at: 2666 2666 Size: 5362 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2621 4369 809 Size: 507 1305 1748 1802 Fragments arranged by size: 1802 1748 1305 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2238 2547 Size: 54 1425 50 275 18 399 9 309 Cuts at: 2547 2809 3698 5327 8 Size: 262 889 1629 43 Fragments arranged by size: 1629 1425 889 399 309 275 262 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2809 1537 Size: 275 997 4090 Fragments arranged by size: 4090 997 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3292 1353 Size: 1939 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3949 4323 4469 387 Size: 1064 138 2360 374 146 1280 Fragments arranged by size: 2360 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2257 2257 Size: 5362 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2294 3425 4022 4809 5077 1239 Size: 393 641 21 1131 597 787 268 1524 Fragments arranged by size: 1524 1131 787 641 597 393 268 21 EcoRI G'AATT_C Cuts at: 2671 3049 2671 Size: 378 4984 FspI TGC'GCA Cuts at: 3868 3868 Size: 5362 HaeII r_GCGC'y Cuts at: 1678 2524 4509 1678 Size: 846 1985 2531 Fragments arranged by size: 2531 1985 846 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2330 2362 4003 Size: 32 135 32 578 32 148 32 1641 Cuts at: 4003 4035 4077 4109 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1641 578 148 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2706 2952 669 Size: 988 281 768 246 3079 Fragments arranged by size: 3079 988 768 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5362 HpaI GTT'AAC Cuts at: 2952 2952 Size: 5362 KpnI G_GTAC'C Cuts at: 2687 2687 Size: 5362 MfeI C'AATT_G Cuts at: 2961 2961 Size: 5362 MluI A'CGCG_T Cuts at: 5238 5238 Size: 5362 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4476 4660 1305 Size: 3171 184 2007 Fragments arranged by size: 3171 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2549 10 Size: 54 1425 343 717 2823 Fragments arranged by size: 2823 1425 717 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2078 3320 3679 3838 518 Size: 648 906 6 1242 359 159 2042 Fragments arranged by size: 2042 1242 906 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2173 2210 2251 2603 2778 3455 4603 4848 Size: 567 37 41 352 175 677 1148 245 Cuts at: 4848 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 567 352 245 175 41 37 NcoI C'CATG_G Cuts at: 513 1065 2260 513 Size: 552 1195 3615 Fragments arranged by size: 3615 1195 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5362 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5362 NotI GC'GGCC_GC Cuts at: 2809 2809 Size: 5362 NspI r_CATG'y Cuts at: 1132 1229 2503 2720 4265 1132 Size: 97 1274 217 1545 2229 Fragments arranged by size: 2229 1545 1274 217 97 PasI CC'CwG_GG Cuts at: 1348 2407 1348 Size: 1059 4303 PciI A'CATG_T Cuts at: 4261 4261 Size: 5362 PflMI CCAn_nnn'nTGG Cuts at: 1108 2227 1108 Size: 1119 4243 PmeI GTTT'AAAC Cuts at: 2666 2666 Size: 5362 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3431 3463 4944 4976 3431 Size: 32 1481 32 3817 Fragments arranged by size: 3817 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5362 PsiI TTA'TAA Cuts at: 2932 2932 Size: 5362 PstI C_TGCA'G Cuts at: 830 2131 2714 830 Size: 1301 583 3478 Fragments arranged by size: 3478 1301 583 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5362 PvuII CAG'CTG Cuts at: 2210 2251 2603 2210 Size: 41 352 4969 Fragments arranged by size: 4969 352 41 SacI G_AGCT'C Cuts at: 2259 2259 Size: 5362 SalI G'TCGA_C Cuts at: 1936 2704 1936 Size: 768 4594 SbfI CC_TGCA'GG Cuts at: 2714 2714 Size: 5362 ScaI AGT'ACT Cuts at: 3610 5322 3610 Size: 1712 3650 SexAI A'CCwGG_T Cuts at: 2586 2586 Size: 5362 SfcI C'TryA_G Cuts at: 826 1045 2127 2710 3845 4526 4717 826 Size: 219 1082 583 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 681 583 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2237 2237 Size: 5362 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5362 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5362 SmaI CCC'GGG Cuts at: 1872 2042 2689 1872 Size: 170 647 4545 Fragments arranged by size: 4545 647 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2566 3466 4367 4629 820 Size: 197 926 623 900 901 262 1553 Fragments arranged by size: 1553 926 901 900 623 262 197 SnaBI TAC'GTA Cuts at: 493 3153 493 Size: 2660 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5362 SphI G_CATG'C Cuts at: 1132 2720 1132 Size: 1588 3774 SspI AAT'ATT Cuts at: 5 52 3286 5 Size: 47 3234 2081 Fragments arranged by size: 3234 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5362 StyI C'CwwG_G Cuts at: 513 1065 2260 2801 513 Size: 552 1195 541 3074 Fragments arranged by size: 3074 1195 552 541 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3389 3406 3559 3744 4189 325 Size: 1255 1809 17 153 185 445 1498 Fragments arranged by size: 1809 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3608 5320 96 Size: 274 80 33 51 1443 1631 1712 138 Fragments arranged by size: 1712 1631 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5322 5354 5322 Size: 32 5330 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5362 XbaI T'CTAG_A Cuts at: 2698 2698 Size: 5362 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2077 2077 Size: 5362 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5362 XmaI C'CCGG_G Cuts at: 1870 2040 2687 1870 Size: 170 647 4545 Fragments arranged by size: 4545 647 170 XmnI GAAnn'nnTTC Cuts at: 3491 3491 Size: 5362 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3170 276 Size: 53 83 186 936 1636 2468 Fragments arranged by size: 2468 1636 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI