(Circular) (Six-base) MAPSORT of: pFN21AB6983.seq Check: 2783 from: 1 to: 5359 pFN21AB6983 5359 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3169 278 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2702 1937 Size: 765 4594 Acc65I G'GTAC_C Cuts at: 2680 2680 Size: 5359 AclI AA'CG_TT Cuts at: 1563 3486 3859 1563 Size: 1923 373 3063 Fragments arranged by size: 3063 1923 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3422 4806 5074 1632 Size: 1790 1384 268 1917 Fragments arranged by size: 1917 1790 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5162 AflIII A'CryG_T Cuts at: 1203 4258 5235 1203 Size: 3055 977 1327 Fragments arranged by size: 3055 1327 977 AgeI A'CCGG_T Cuts at: 4190 4190 Size: 5359 AhdI GACnn_n'nnGTC Cuts at: 4088 4088 Size: 5359 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5359 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5359 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2580 2612 2580 Size: 32 5327 AlwNI CAG_nnn'CTG Cuts at: 2383 3102 4674 2383 Size: 719 1572 3068 Fragments arranged by size: 3068 1572 719 ApaI G_GGCC'C Cuts at: 2067 2067 Size: 5359 ApaLI G'TGCA_C Cuts at: 3416 4572 3416 Size: 1156 4203 ApoI r'AATT_y Cuts at: 1493 2668 2894 3046 5155 1493 Size: 1175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 226 152 AseI AT'TA_AT Cuts at: 160 1032 2334 3913 160 Size: 872 1302 1579 1606 Fragments arranged by size: 1606 1579 1302 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 AvaI C'yCGr_G Cuts at: 1870 1943 2046 2066 2684 1870 Size: 73 103 20 618 4545 Fragments arranged by size: 4545 618 103 73 20 BamHI G'GATC_C Cuts at: 2689 3056 2689 Size: 367 4992 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2486 2680 4135 618 Size: 325 217 627 699 194 1455 1842 Fragments arranged by size: 1842 1455 699 627 325 217 194 BanII G_rGCy'C Cuts at: 1391 2067 2598 2609 2678 1391 Size: 676 531 11 69 4072 Fragments arranged by size: 4072 676 531 69 11 BbeI G_GCGC'C Cuts at: 2490 2490 Size: 5359 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3550 3584 1903 Size: 34 1613 34 3678 Fragments arranged by size: 3678 1613 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3251 4461 3251 Size: 1210 4149 BclI T'GATC_A Cuts at: 1548 1548 Size: 5359 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3970 136 Size: 107 122 71 631 605 2298 1525 Fragments arranged by size: 2298 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2208 2437 5354 2208 Size: 229 2917 2213 Fragments arranged by size: 2917 2213 229 BlpI GC'TnA_GC Cuts at: 2776 2776 Size: 5359 Bme1580I G_kGCm'C Cuts at: 1790 2067 2620 3420 4576 1790 Size: 277 553 800 1156 2573 Fragments arranged by size: 2573 1156 800 553 277 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5359 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2607 3090 4048 Size: 416 53 83 399 24 1190 483 958 Cuts at: 4048 442 Size: 1753 Fragments arranged by size: 1753 1190 958 483 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 4019 1239 Size: 1223 1557 2579 Fragments arranged by size: 2579 1557 1223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3484 4349 4647 3484 Size: 865 298 4196 Fragments arranged by size: 4196 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4022 882 Size: 617 2523 2219 Fragments arranged by size: 2523 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 3150 493 Size: 711 1296 650 2702 Fragments arranged by size: 2702 1296 711 650 BsaBI GATnn'nnATC Cuts at: 5353 5353 Size: 5359 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 2487 3166 3548 Size: 53 83 186 936 488 466 679 382 Cuts at: 3548 4183 275 Size: 635 1451 Fragments arranged by size: 1451 936 679 635 488 466 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2574 3792 4190 4464 4611 5022 2574 Size: 1218 398 274 147 411 2911 Fragments arranged by size: 2911 1218 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2580 2610 1178 Size: 30 165 30 1177 30 3927 Fragments arranged by size: 3927 1177 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5359 BseYI C'CCAG_C Cuts at: 2081 2230 4562 5058 2081 Size: 149 2332 496 2382 Fragments arranged by size: 2382 2332 496 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4786 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2809 3570 4598 1540 Size: 275 186 808 761 1028 2301 Fragments arranged by size: 2301 1028 808 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2598 3420 3505 4576 2598 Size: 822 85 1071 3381 Fragments arranged by size: 3381 1071 822 85 BsmI GAATG_Cn' Cuts at: 2870 2963 2870 Size: 93 5266 BsmBI CGTCTCn'nnnn_ Cuts at: 2537 2537 Size: 5359 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2598 2609 2620 2678 3420 3505 Size: 399 277 531 11 11 58 742 85 Cuts at: 3505 4576 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 277 85 58 11 11 BspEI T'CCGG_A Cuts at: 2574 5022 2574 Size: 2448 2911 BspHI T'CATG_A Cuts at: 3248 4978 3248 Size: 1730 3629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2714 844 Size: 1870 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3246 1394 Size: 429 1423 3507 Fragments arranged by size: 3507 1423 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 4028 65 Size: 1183 2606 174 1396 Fragments arranged by size: 2606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2340 4003 4190 1700 Size: 110 530 1663 187 2869 Fragments arranged by size: 2869 1663 530 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5359 BssHII G'CGCG_C Cuts at: 1925 2133 1925 Size: 208 5151 BssSI C'ACGA_G Cuts at: 3419 4431 3419 Size: 1012 4347 BstAPI GCAn_nnn'nTGC Cuts at: 794 2296 2383 2563 794 Size: 1502 87 180 3590 Fragments arranged by size: 3590 1502 180 87 BstBI TT'CG_AA Cuts at: 4163 4163 Size: 5359 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5359 BstYI r'GATC_y Cuts at: 1972 2208 2437 2689 3056 3441 3458 4910 4996 Size: 236 229 252 367 385 17 1452 86 Cuts at: 4996 5008 5354 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 252 236 229 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2256 2308 513 Size: 552 1191 52 3564 Fragments arranged by size: 3564 1191 552 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4620 Fragments arranged by size: 4620 683 56 BtsI GCAGTG_nn' Cuts at: 2221 2875 3669 3689 2221 Size: 654 794 20 3891 Fragments arranged by size: 3891 794 654 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5359 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5324 DraI TTT'AAA Cuts at: 2663 2663 Size: 5359 DraIII CAC_nnn'GTG Cuts at: 2500 2500 Size: 5359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4366 809 Size: 507 3050 1802 Fragments arranged by size: 3050 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2305 2520 2806 Size: 54 1425 50 275 18 475 215 286 Cuts at: 2806 3695 5324 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 475 286 275 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2806 1537 Size: 275 994 4090 Fragments arranged by size: 4090 994 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2375 3289 1353 Size: 909 113 914 3423 Fragments arranged by size: 3423 914 909 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3946 4320 4466 387 Size: 1064 138 2357 374 146 1280 Fragments arranged by size: 2357 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2596 2596 Size: 5359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 3422 4019 4806 5074 1239 Size: 393 830 960 597 787 268 1524 Fragments arranged by size: 1524 960 830 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2057 2071 2458 2057 Size: 14 387 4958 Fragments arranged by size: 4958 387 14 EcoRI G'AATT_C Cuts at: 2668 3046 2668 Size: 378 4981 FspI TGC'GCA Cuts at: 3865 3865 Size: 5359 HaeII r_GCGC'y Cuts at: 1678 2455 2490 4506 1678 Size: 777 35 2016 2531 Fragments arranged by size: 2531 2016 777 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 4000 4032 4074 Size: 32 135 32 857 32 1539 32 42 Cuts at: 4074 4106 1373 Size: 32 2626 Fragments arranged by size: 2626 1539 857 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2949 669 Size: 988 281 765 246 3079 Fragments arranged by size: 3079 988 765 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5359 HpaI GTT'AAC Cuts at: 2949 2949 Size: 5359 KasI G'GCGC_C Cuts at: 2486 2486 Size: 5359 KpnI G_GTAC'C Cuts at: 2684 2684 Size: 5359 MfeI C'AATT_G Cuts at: 2958 2958 Size: 5359 MluI A'CGCG_T Cuts at: 5235 5235 Size: 5359 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4473 4657 1305 Size: 3168 184 2007 Fragments arranged by size: 3168 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2307 10 Size: 54 1425 343 475 3062 Fragments arranged by size: 3062 1425 475 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3317 3676 3835 518 Size: 648 2151 359 159 2042 Fragments arranged by size: 2151 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2258 2560 2775 3452 4600 4845 1606 Size: 407 245 302 215 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 407 302 245 245 215 NarI GG'CG_CC Cuts at: 2487 2487 Size: 5359 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4807 NdeI CA'TA_TG Cuts at: 387 387 Size: 5359 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5359 NotI GC'GGCC_GC Cuts at: 2806 2806 Size: 5359 NspI r_CATG'y Cuts at: 1132 1229 2299 2717 4262 1132 Size: 97 1070 418 1545 2229 Fragments arranged by size: 2229 1545 1070 418 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5359 PciI A'CATG_T Cuts at: 4258 4258 Size: 5359 PflMI CCAn_nnn'nTGG Cuts at: 1108 2500 1108 Size: 1392 3967 PfoI T'CCnGG_A Cuts at: 2024 2247 2024 Size: 223 5136 PmeI GTTT'AAAC Cuts at: 2663 2663 Size: 5359 PmlI CAC'GTG Cuts at: 2500 2500 Size: 5359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2580 2612 3428 3460 4941 4973 2580 Size: 32 816 32 1481 32 2966 Fragments arranged by size: 2966 1481 816 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2071 2458 2057 Size: 14 387 4958 Fragments arranged by size: 4958 387 14 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5359 PsiI TTA'TAA Cuts at: 2929 2929 Size: 5359 PspOMI G'GGCC_C Cuts at: 2063 2063 Size: 5359 PstI C_TGCA'G Cuts at: 830 2216 2711 830 Size: 1386 495 3478 Fragments arranged by size: 3478 1386 495 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5359 PvuII CAG'CTG Cuts at: 2560 2560 Size: 5359 SacI G_AGCT'C Cuts at: 2598 2598 Size: 5359 SacII CC_GC'GG Cuts at: 2259 2259 Size: 5359 SalI G'TCGA_C Cuts at: 1936 2701 1936 Size: 765 4594 SanDI GG'GwC_CC Cuts at: 2071 2071 Size: 5359 SapI GCTCTTCn'nnn_ Cuts at: 2375 2375 Size: 5359 SbfI CC_TGCA'GG Cuts at: 2711 2711 Size: 5359 ScaI AGT'ACT Cuts at: 3607 5319 3607 Size: 1712 3647 SfcI C'TryA_G Cuts at: 826 1045 2212 2707 3842 4523 4714 826 Size: 219 1167 495 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 681 495 219 191 SfoI GGC'GCC Cuts at: 2488 2488 Size: 5359 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5359 SmaI CCC'GGG Cuts at: 1872 2048 2068 2686 1872 Size: 176 20 618 4545 Fragments arranged by size: 4545 618 176 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 3463 4364 4626 820 Size: 197 926 1520 901 262 1553 Fragments arranged by size: 1553 1520 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3150 493 Size: 2657 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5359 SphI G_CATG'C Cuts at: 1132 2717 1132 Size: 1585 3774 SspI AAT'ATT Cuts at: 5 52 3283 5 Size: 47 3231 2081 Fragments arranged by size: 3231 2081 47 StuI AGG'CCT Cuts at: 1517 2516 1517 Size: 999 4360 StyI C'CwwG_G Cuts at: 513 1065 2798 513 Size: 552 1733 3074 Fragments arranged by size: 3074 1733 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 3403 3556 3741 4186 325 Size: 1255 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 3605 5317 Size: 274 80 33 51 1443 315 1313 1712 Cuts at: 5317 96 Size: 138 Fragments arranged by size: 1712 1443 1313 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5319 5351 5319 Size: 32 5327 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5359 XbaI T'CTAG_A Cuts at: 2695 2695 Size: 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5359 XmaI C'CCGG_G Cuts at: 1870 2046 2066 2684 1870 Size: 176 20 618 4545 Fragments arranged by size: 4545 618 176 20 XmnI GAAnn'nnTTC Cuts at: 3488 3488 Size: 5359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3167 276 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI