(Circular) (Six-base) MAPSORT of: pFN21AB6980.seq Check: 4534 from: 1 to: 5440 pFN21AB6980 5440 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2371 2371 Size: 5440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2160 2783 1937 Size: 223 623 4594 Fragments arranged by size: 4594 623 223 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2230 3503 4887 5155 1632 Size: 598 1273 1384 268 1917 Fragments arranged by size: 1917 1384 1273 598 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 2287 4169 2287 Size: 1882 3558 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 3183 4755 3183 Size: 1572 3868 ApaI G_GGCC'C Cuts at: 2215 2215 Size: 5440 ApaLI G'TGCA_C Cuts at: 2675 3497 4653 2675 Size: 822 1156 3462 Fragments arranged by size: 3462 1156 822 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AscI GG'CGCG_CC Cuts at: 2056 2056 Size: 5440 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2187 2193 2667 2765 1870 Size: 73 244 6 474 98 4545 Fragments arranged by size: 4545 474 244 98 73 6 BamHI G'GATC_C Cuts at: 2770 3137 2770 Size: 367 5073 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2761 4216 618 Size: 325 217 627 974 1455 1842 Fragments arranged by size: 1842 1455 974 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2186 2194 2200 2215 2759 1391 Size: 631 164 8 6 15 544 4072 Fragments arranged by size: 4072 631 544 164 15 8 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4469 BbvCI CC'TCA_GC Cuts at: 2522 2522 Size: 5440 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2448 3332 4542 2448 Size: 884 1210 3346 Fragments arranged by size: 3346 1210 884 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2435 4051 136 Size: 107 122 71 631 605 763 1616 1525 Fragments arranged by size: 1616 1525 763 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2485 2857 2485 Size: 372 5068 Bme1580I G_kGCm'C Cuts at: 1790 2215 2679 3501 4657 1790 Size: 425 464 822 1156 2573 Fragments arranged by size: 2573 1156 822 464 425 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2654 4100 1239 Size: 797 618 1446 2579 Fragments arranged by size: 2579 1446 797 618 Bpu10I CC'TnA_GC Cuts at: 2522 2522 Size: 5440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2621 3565 4430 4728 2621 Size: 944 865 298 3333 Fragments arranged by size: 3333 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 2369 5434 2369 Size: 3065 2375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3247 3629 4264 275 Size: 53 83 186 936 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2342 2372 2627 Size: 30 165 30 606 30 303 30 255 Cuts at: 2627 2657 1178 Size: 30 3961 Fragments arranged by size: 3961 606 303 255 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5440 BseYI C'CCAG_C Cuts at: 2553 2731 4643 5139 2553 Size: 178 1912 496 2854 Fragments arranged by size: 2854 1912 496 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 2399 2603 1163 Size: 573 597 66 204 4000 Fragments arranged by size: 4000 597 573 204 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2890 3651 4679 1540 Size: 275 186 889 761 1028 2301 Fragments arranged by size: 2301 1028 889 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2486 2679 3501 3586 4657 2022 Size: 464 193 822 85 1071 2805 Fragments arranged by size: 2805 1071 822 464 193 85 BsmI GAATG_Cn' Cuts at: 2428 2951 3044 2428 Size: 523 93 4824 Fragments arranged by size: 4824 523 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2186 2194 2200 2215 2486 2679 Size: 399 232 164 8 6 15 271 193 Cuts at: 2679 2759 3501 3586 4657 1391 Size: 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 271 232 193 164 85 80 15 8 6 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2371 2795 844 Size: 1527 424 3489 Fragments arranged by size: 3489 1527 424 BsrBI CCG'CTC Cuts at: 1394 1823 2638 3327 1394 Size: 429 815 689 3507 Fragments arranged by size: 3507 815 689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3935 4109 65 Size: 1183 2687 174 1396 Fragments arranged by size: 2687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 2056 1925 Size: 131 5309 BssSI C'ACGA_G Cuts at: 2171 2621 3500 4512 2171 Size: 450 879 1012 3099 Fragments arranged by size: 3099 1012 879 450 BstAPI GCAn_nnn'nTGC Cuts at: 794 2710 794 Size: 1916 3524 BstBI TT'CG_AA Cuts at: 2325 4244 2325 Size: 1919 3521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstXI CCAn_nnnn'nTGG Cuts at: 2149 2149 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2076 2175 2770 3137 3522 3539 4991 5077 Size: 104 99 595 367 385 17 1452 86 Cuts at: 5077 5089 5435 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 595 385 367 346 104 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2581 2581 Size: 5440 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3949 Fragments arranged by size: 3949 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2445 2956 3750 3770 2445 Size: 511 794 20 4115 Fragments arranged by size: 4115 794 511 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2270 2887 3776 Size: 54 1425 50 275 18 440 617 889 Cuts at: 3776 5405 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 617 440 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2887 1537 Size: 275 1075 4090 Fragments arranged by size: 4090 1075 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3370 1353 Size: 2017 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2303 2531 4027 4401 4547 387 Size: 1064 138 714 228 1496 374 146 1280 Fragments arranged by size: 1496 1280 1064 714 374 228 146 138 EcoICRI GAG'CTC Cuts at: 2020 2020 Size: 5440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2230 2654 3503 4100 4887 5155 Size: 393 404 194 424 849 597 787 268 Cuts at: 5155 1239 Size: 1524 Fragments arranged by size: 1524 849 787 597 424 404 393 268 194 EcoO109I rG'GnC_Cy Cuts at: 2211 2212 2403 2211 Size: 1 191 5248 Fragments arranged by size: 5248 191 1 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 EcoRV GAT'ATC Cuts at: 2371 2371 Size: 5440 FspI TGC'GCA Cuts at: 2072 3946 2072 Size: 1874 3566 HaeII r_GCGC'y Cuts at: 1678 4587 1678 Size: 2909 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2273 2305 4081 Size: 32 135 32 437 32 232 32 1776 Cuts at: 4081 4113 4155 4187 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1776 437 232 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 2625 4554 4738 1305 Size: 702 618 1929 184 2007 Fragments arranged by size: 2007 1929 702 618 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3618 Fragments arranged by size: 3618 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2712 3398 3757 3916 518 Size: 648 1546 686 359 159 2042 Fragments arranged by size: 2042 1546 686 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2347 2489 2543 2557 2856 3533 4681 4926 Size: 741 142 54 14 299 677 1148 245 Cuts at: 4926 1606 Size: 2120 Fragments arranged by size: 2120 1148 741 677 299 245 142 54 14 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3949 Fragments arranged by size: 3949 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2711 2798 4343 1132 Size: 97 1482 87 1545 2229 Fragments arranged by size: 2229 1545 1482 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5440 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2403 2403 Size: 5440 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PspOMI G'GGCC_C Cuts at: 2211 2211 Size: 5440 PspXI vC'TCGA_Gb Cuts at: 2193 2193 Size: 5440 PstI C_TGCA'G Cuts at: 830 2310 2792 830 Size: 1480 482 3478 Fragments arranged by size: 3478 1480 482 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 PvuII CAG'CTG Cuts at: 2347 2489 2543 2557 2347 Size: 142 54 14 5230 Fragments arranged by size: 5230 142 54 14 SacI G_AGCT'C Cuts at: 2022 2022 Size: 5440 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2306 2387 2788 3923 4604 4795 826 Size: 219 1261 81 401 1135 681 191 1471 Fragments arranged by size: 1471 1261 1135 681 401 219 191 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2189 2767 1872 Size: 317 578 4545 Fragments arranged by size: 4545 578 317 SmlI C'TyrA_G Cuts at: 820 1017 1943 2193 2600 3544 4445 4707 820 Size: 197 926 250 407 944 901 262 1553 Fragments arranged by size: 1553 944 926 901 407 262 250 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 2720 1517 Size: 1203 4237 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 2879 513 Size: 552 939 138 737 3074 Fragments arranged by size: 3074 939 737 552 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2367 3467 3484 3637 3822 4267 325 Size: 1255 787 1100 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1100 787 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2374 5400 5432 2342 Size: 32 3026 32 2350 Fragments arranged by size: 3026 2350 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 2193 1943 Size: 250 5190 XmaI C'CCGG_G Cuts at: 1870 2187 2765 1870 Size: 317 578 4545 Fragments arranged by size: 4545 578 317 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI