(Circular) (Six-base) MAPSORT of: pFN21AB6970.seq Check: 6060 from: 1 to: 5428 pFN21AB6970 5428 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 3491 4875 5143 1632 Size: 876 983 1384 268 1917 Fragments arranged by size: 1917 1384 983 876 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 2685 4327 5304 1203 Size: 1482 1642 977 1327 Fragments arranged by size: 1642 1482 1327 977 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5428 AlwNI CAG_nnn'CTG Cuts at: 3171 4743 3171 Size: 1572 3856 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2677 2737 2963 3115 5224 1493 Size: 1184 60 226 152 2109 1697 Fragments arranged by size: 2109 1697 1184 226 152 60 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2505 2753 1870 Size: 73 562 248 4545 Fragments arranged by size: 4545 562 248 73 BamHI G'GATC_C Cuts at: 2121 2758 3125 2121 Size: 637 367 4424 Fragments arranged by size: 4424 637 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 2531 2749 4204 618 Size: 325 217 627 609 135 218 1455 1842 Fragments arranged by size: 1842 1455 627 609 325 218 217 135 BanII G_rGCy'C Cuts at: 1391 2187 2543 2747 1391 Size: 796 356 204 4072 Fragments arranged by size: 4072 796 356 204 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2586 928 Size: 971 687 3770 Fragments arranged by size: 3770 971 687 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3619 3653 1903 Size: 34 1682 34 3678 Fragments arranged by size: 3678 1682 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4530 3320 Size: 1210 4218 BclI T'GATC_A Cuts at: 1548 1548 Size: 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 4039 136 Size: 107 122 71 631 605 418 1949 1525 Fragments arranged by size: 1949 1525 631 605 418 122 107 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 2536 3489 4645 1790 Size: 746 953 1156 2573 Fragments arranged by size: 2573 1156 953 746 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3159 4117 442 Size: 416 53 83 399 24 1742 958 1753 Fragments arranged by size: 1753 1742 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2657 1056 Size: 1601 3827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4088 1239 Size: 2849 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3553 4418 4716 3553 Size: 865 298 4265 Fragments arranged by size: 4265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4091 882 Size: 617 2592 2219 Fragments arranged by size: 2592 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3219 493 Size: 711 2015 2702 Fragments arranged by size: 2702 2015 711 BsaBI GATnn'nnATC Cuts at: 2388 5422 2388 Size: 3034 2394 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3235 3617 4252 275 Size: 53 83 186 936 1702 382 635 1451 Fragments arranged by size: 1702 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2035 2065 1178 Size: 30 165 30 632 30 4541 Fragments arranged by size: 4541 632 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2451 2639 1186 Size: 1265 188 3975 Fragments arranged by size: 3975 1265 188 BseYI C'CCAG_C Cuts at: 2186 2457 4631 5127 2186 Size: 271 2174 496 2487 Fragments arranged by size: 2487 2174 496 271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 1163 Size: 573 948 3907 Fragments arranged by size: 3907 948 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2878 3639 4667 1540 Size: 275 186 877 761 1028 2301 Fragments arranged by size: 2301 1028 877 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2543 3489 3574 4645 2543 Size: 946 85 1071 3326 Fragments arranged by size: 3326 1071 946 85 BsmI GAATG_Cn' Cuts at: 2641 2939 3032 2641 Size: 298 93 5037 Fragments arranged by size: 5037 298 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2536 2543 2747 3489 3574 4645 Size: 399 397 349 7 204 742 85 1071 Cuts at: 4645 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 397 349 204 85 7 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2783 844 Size: 1939 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3315 1394 Size: 429 1492 3507 Fragments arranged by size: 3507 1492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3923 4097 65 Size: 1183 2675 174 1396 Fragments arranged by size: 2675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4072 4259 1700 Size: 110 2262 187 2869 Fragments arranged by size: 2869 2262 187 110 BsrGI T'GTAC_A Cuts at: 96 2491 96 Size: 2395 3033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 3488 4500 3488 Size: 1012 4416 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5428 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2121 2389 2758 3125 3510 3527 4979 5065 Size: 149 268 369 367 385 17 1452 86 Cuts at: 5065 5077 5423 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 369 367 346 268 149 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4876 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3738 3758 2944 Size: 794 20 4614 Fragments arranged by size: 4614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2310 2345 529 Size: 35 1746 35 3612 Fragments arranged by size: 3612 1746 35 35 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4435 809 Size: 507 3119 1802 Fragments arranged by size: 3119 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2671 2875 3764 Size: 54 1425 50 275 18 841 204 889 Cuts at: 3764 5393 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 841 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2875 1537 Size: 275 1063 4090 Fragments arranged by size: 4090 1063 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2703 3358 1353 Size: 800 550 655 3423 Fragments arranged by size: 3423 800 655 550 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4015 4389 4535 387 Size: 1064 138 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2541 2541 Size: 5428 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 3491 4088 4875 5143 1239 Size: 393 876 983 597 787 268 1524 Fragments arranged by size: 1524 983 876 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2453 2484 2453 Size: 31 5397 EcoRI G'AATT_C Cuts at: 2737 3115 2737 Size: 378 5050 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 4575 1678 Size: 2897 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2203 2235 4069 4101 4143 Size: 32 135 32 631 32 1834 32 42 Cuts at: 4143 4175 1373 Size: 32 2626 Fragments arranged by size: 2626 1834 631 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2772 3018 669 Size: 988 281 834 246 3079 Fragments arranged by size: 3079 988 834 281 246 HindIII A'AGCT_T Cuts at: 748 2099 748 Size: 1351 4077 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 2511 3027 2511 Size: 516 4912 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4542 4726 1305 Size: 3237 184 2007 Fragments arranged by size: 3237 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2673 10 Size: 54 1425 343 841 2765 Fragments arranged by size: 2765 1425 841 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3386 3745 3904 518 Size: 648 2220 359 159 2042 Fragments arranged by size: 2220 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2461 2844 3521 4669 4914 1606 Size: 855 383 677 1148 245 2120 Fragments arranged by size: 2120 1148 855 677 383 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4876 NdeI CA'TA_TG Cuts at: 387 2469 387 Size: 2082 3346 NheI G'CTAG_C Cuts at: 1052 2653 1052 Size: 1601 3827 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2689 2786 4331 1132 Size: 97 1460 97 1545 2229 Fragments arranged by size: 2229 1545 1460 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5428 PciI A'CATG_T Cuts at: 2685 4327 2685 Size: 1642 3786 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5428 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2453 2484 2453 Size: 31 5397 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PspXI vC'TCGA_Gb Cuts at: 2505 2505 Size: 5428 PstI C_TGCA'G Cuts at: 830 2780 830 Size: 1950 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5428 SacI G_AGCT'C Cuts at: 2543 2543 Size: 5428 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2109 2776 3911 4592 4783 826 Size: 219 1064 667 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 681 667 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2755 1872 Size: 883 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2505 3532 4433 4695 820 Size: 197 926 562 1027 901 262 1553 Fragments arranged by size: 1553 1027 926 901 562 262 197 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2786 1132 Size: 1654 3774 SspI AAT'ATT Cuts at: 5 52 3352 5 Size: 47 3300 2081 Fragments arranged by size: 3300 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5428 StyI C'CwwG_G Cuts at: 513 1065 2867 513 Size: 552 1802 3074 Fragments arranged by size: 3074 1802 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3455 3472 3625 3810 4255 325 Size: 1255 1875 17 153 185 445 1498 Fragments arranged by size: 1875 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2491 3674 5386 Size: 274 80 33 51 1443 514 1183 1712 Cuts at: 5386 96 Size: 138 Fragments arranged by size: 1712 1443 1183 514 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2589 2621 5388 5420 2589 Size: 32 2767 32 2597 Fragments arranged by size: 2767 2597 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 2505 1943 Size: 562 4866 XmaI C'CCGG_G Cuts at: 1870 2753 1870 Size: 883 4545 XmnI GAAnn'nnTTC Cuts at: 2167 3557 2167 Size: 1390 4038 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI