(Circular) (Six-base) MAPSORT of: pFN21AB9870.seq Check: 8818 from: 1 to: 6136 pFN21AB9870 6136 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2223 2223 Size: 6136 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2349 3033 3335 3946 Size: 53 83 186 936 813 684 302 611 Cuts at: 3946 278 Size: 2468 Fragments arranged by size: 2468 936 813 684 611 302 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4199 5583 5851 1632 Size: 2567 1384 268 1917 Fragments arranged by size: 2567 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 3152 1166 Size: 1986 4150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2642 2674 2642 Size: 32 6104 AlwNI CAG_nnn'CTG Cuts at: 2065 3121 3879 5451 2065 Size: 1056 758 1572 2750 Fragments arranged by size: 2750 1572 1056 758 ApaI G_GGCC'C Cuts at: 2118 2118 Size: 6136 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 3445 3671 3823 5932 1493 Size: 1952 226 152 2109 1697 Fragments arranged by size: 2109 1952 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2038 3461 1870 Size: 73 95 1423 4545 Fragments arranged by size: 4545 1423 95 73 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2101 3457 4912 618 Size: 325 217 627 240 74 1356 1455 1842 Fragments arranged by size: 1842 1455 1356 627 325 240 217 74 BanII G_rGCy'C Cuts at: 1391 2093 2118 2634 3183 3455 1391 Size: 702 25 516 549 272 4072 Fragments arranged by size: 4072 702 549 516 272 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2230 2908 3025 3199 928 Size: 971 331 678 117 174 3865 Fragments arranged by size: 3865 971 678 331 174 117 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2624 2658 4327 4361 1903 Size: 34 50 34 603 34 1669 34 3678 Fragments arranged by size: 3678 1669 603 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 2904 1548 Size: 1356 4780 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2113 2312 4747 Size: 107 122 71 631 605 441 199 2435 Cuts at: 4747 136 Size: 1525 Fragments arranged by size: 2435 1525 631 605 441 199 122 107 71 BglII A'GATC_T Cuts at: 3321 6131 3321 Size: 2810 3326 BlpI GC'TnA_GC Cuts at: 3553 3553 Size: 6136 Bme1580I G_kGCm'C Cuts at: 1790 2104 2118 4197 5353 1790 Size: 314 14 2079 1156 2573 Fragments arranged by size: 2573 2079 1156 314 14 BmgBI CAC'GTC Cuts at: 1318 2638 1318 Size: 1320 4816 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2120 2431 3867 Size: 416 53 83 399 24 703 311 1436 Cuts at: 3867 4825 442 Size: 958 1753 Fragments arranged by size: 1753 1436 958 703 416 399 311 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3202 3202 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2416 2644 2810 4796 1239 Size: 1177 228 166 1986 2579 Fragments arranged by size: 2579 1986 1177 228 166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2873 4261 5126 5424 2873 Size: 1388 865 298 3585 Fragments arranged by size: 3585 1388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2870 3011 4799 882 Size: 617 1371 141 1788 2219 Fragments arranged by size: 2219 1788 1371 617 141 BsaAI yAC'GTr Cuts at: 493 1204 3927 493 Size: 711 2723 2702 Fragments arranged by size: 2723 2702 711 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2346 2606 3030 3332 Size: 53 83 186 936 813 260 424 302 Cuts at: 3332 3943 4325 4960 275 Size: 611 382 635 1451 Fragments arranged by size: 1451 936 813 635 611 424 382 302 260 186 83 53 BsaWI w'CCGG_w Cuts at: 2527 4569 4967 5241 5388 5799 2527 Size: 2042 398 274 147 411 2864 Fragments arranged by size: 2864 2042 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2642 2672 3125 3155 1178 Size: 30 165 30 1239 30 453 30 4159 Fragments arranged by size: 4159 1239 453 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 3049 3305 3308 1186 Size: 1444 419 256 3 4014 Fragments arranged by size: 4014 1444 419 256 3 BseYI C'CCAG_C Cuts at: 2170 3048 3114 3121 5339 5835 2170 Size: 878 66 7 2218 496 2471 Fragments arranged by size: 2471 2218 878 496 66 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2786 3148 1163 Size: 573 1050 362 4151 Fragments arranged by size: 4151 1050 573 362 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2997 3310 3586 4347 5375 1540 Size: 275 186 996 313 276 761 1028 2301 Fragments arranged by size: 2301 1028 996 761 313 276 275 186 BsiHKAI G_wGCw'C Cuts at: 2375 2808 3183 4197 4282 5353 2375 Size: 433 375 1014 85 1071 3158 Fragments arranged by size: 3158 1071 1014 433 375 85 BsmI GAATG_Cn' Cuts at: 2647 3647 3740 2647 Size: 1000 93 5043 Fragments arranged by size: 5043 1000 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3152 3152 Size: 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2104 2118 2375 2634 2808 3183 Size: 399 303 11 14 257 259 174 375 Cuts at: 3183 3455 4197 4282 5353 1391 Size: 272 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 303 272 259 257 174 85 14 11 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 2724 4025 5755 2724 Size: 1301 1730 3105 Fragments arranged by size: 3105 1730 1301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 2589 3491 844 Size: 1379 366 902 3489 Fragments arranged by size: 3489 1379 902 366 BsrBI CCG'CTC Cuts at: 1394 1823 4023 1394 Size: 429 2200 3507 Fragments arranged by size: 3507 2200 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3181 4631 4805 65 Size: 1183 1933 1450 174 1396 Fragments arranged by size: 1933 1450 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2315 2996 3151 4780 4967 1700 Size: 110 505 681 155 1629 187 2869 Fragments arranged by size: 2869 1629 681 505 187 155 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 2218 2838 2857 1925 Size: 293 620 19 5204 Fragments arranged by size: 5204 620 293 19 BssSI C'ACGA_G Cuts at: 4196 5208 4196 Size: 1012 5124 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6136 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 2128 3344 1184 Size: 944 1216 3976 Fragments arranged by size: 3976 1216 944 BstYI r'GATC_y Cuts at: 1972 2769 3321 3466 3833 4218 4235 5687 5773 Size: 797 552 145 367 385 17 1452 86 Cuts at: 5773 5785 6131 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 797 552 385 367 346 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2082 513 Size: 552 1017 4567 Fragments arranged by size: 4567 1017 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2740 487 Size: 683 56 1514 3883 Fragments arranged by size: 3883 1514 683 56 BtsI GCAGTG_nn' Cuts at: 2376 2977 3652 4446 4466 2376 Size: 601 675 794 20 4046 Fragments arranged by size: 4046 794 675 601 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3035 5143 809 Size: 507 1719 2108 1802 Fragments arranged by size: 2108 1802 1719 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2313 2595 Size: 54 1425 50 275 18 423 60 282 Cuts at: 2595 2994 3583 4472 6101 8 Size: 399 589 889 1629 43 Fragments arranged by size: 1629 1425 889 589 423 399 282 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2994 3583 1537 Size: 275 1182 589 4090 Fragments arranged by size: 4090 1182 589 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2692 4066 1353 Size: 1339 1374 3423 Fragments arranged by size: 3423 1374 1339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4723 5097 5243 387 Size: 1064 138 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3181 3181 Size: 6136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2416 2644 2810 4199 4796 5583 5851 Size: 393 784 228 166 1389 597 787 268 Cuts at: 5851 1239 Size: 1524 Fragments arranged by size: 1524 1389 787 784 597 393 268 228 166 EcoO109I rG'GnC_Cy Cuts at: 2114 2115 2436 2987 3299 2114 Size: 1 321 551 312 4951 Fragments arranged by size: 4951 551 321 312 1 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FspI TGC'GCA Cuts at: 2191 4642 2191 Size: 2451 3685 HaeII r_GCGC'y Cuts at: 1678 2279 5283 1678 Size: 601 3004 2531 Fragments arranged by size: 3004 2531 601 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4777 4809 4851 4883 1373 Size: 32 135 32 3205 32 42 32 2626 Fragments arranged by size: 3205 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6136 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5250 5434 1305 Size: 3945 184 2007 Fragments arranged by size: 3945 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2597 10 Size: 54 1425 343 765 3549 Fragments arranged by size: 3549 1425 765 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 2729 3152 3239 4094 4453 4612 Size: 648 1242 321 423 87 855 359 159 Cuts at: 4612 518 Size: 2042 Fragments arranged by size: 2042 1242 855 648 423 359 321 159 87 MspA1I CmG'CkG Cuts at: 1606 2065 3118 3227 3552 4229 5377 5622 1606 Size: 459 1053 109 325 677 1148 245 2120 Fragments arranged by size: 2120 1148 1053 677 459 325 245 109 NaeI GCC'GGC Cuts at: 2998 2998 Size: 6136 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5584 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NgoMIV G'CCGG_C Cuts at: 2996 2996 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3316 3494 5039 1132 Size: 97 2087 178 1545 2229 Fragments arranged by size: 2229 2087 1545 178 97 PasI CC'CwG_GG Cuts at: 1348 2610 1348 Size: 1262 4874 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 3121 1108 Size: 957 1056 4123 Fragments arranged by size: 4123 1056 957 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2642 2674 4205 4237 5718 5750 2642 Size: 32 1531 32 1481 32 3028 Fragments arranged by size: 3028 1531 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3299 3299 Size: 6136 PshAI GACnn'nnGTC Cuts at: 1613 2234 1613 Size: 621 5515 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PspOMI G'GGCC_C Cuts at: 2114 2114 Size: 6136 PstI C_TGCA'G Cuts at: 830 2972 3488 830 Size: 2142 516 3478 Fragments arranged by size: 3478 2142 516 PvuI CG_AT'CG Cuts at: 2001 3310 2001 Size: 1309 4827 PvuII CAG'CTG Cuts at: 2065 3118 3227 2065 Size: 1053 109 4974 Fragments arranged by size: 4974 1053 109 SacI G_AGCT'C Cuts at: 3183 3183 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 2968 3484 4619 5300 5491 826 Size: 219 1923 516 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 516 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 3151 1700 Size: 1451 4685 SmaI CCC'GGG Cuts at: 1872 2040 3463 1872 Size: 168 1423 4545 Fragments arranged by size: 4545 1423 168 SmlI C'TyrA_G Cuts at: 820 1017 1943 2888 4240 5141 5403 820 Size: 197 926 945 1352 901 262 1553 Fragments arranged by size: 1553 1352 945 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2475 2880 3575 513 Size: 552 1410 405 695 3074 Fragments arranged by size: 3074 1410 695 552 405 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 4163 4180 4333 4518 4963 325 Size: 1255 761 1822 17 153 185 445 1498 Fragments arranged by size: 1822 1498 1255 761 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4382 6094 96 Size: 274 80 33 51 1443 2405 1712 138 Fragments arranged by size: 2405 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3125 3157 6096 6128 3125 Size: 32 2939 32 3133 Fragments arranged by size: 3133 2939 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2503 2896 3203 1316 Size: 1187 393 307 4249 Fragments arranged by size: 4249 1187 393 307 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 2038 3461 1870 Size: 168 1423 4545 Fragments arranged by size: 4545 1423 168 XmnI GAAnn'nnTTC Cuts at: 3428 4265 3428 Size: 837 5299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2347 3031 3333 3944 Size: 53 83 186 936 813 684 302 611 Cuts at: 3944 276 Size: 2468 Fragments arranged by size: 2468 936 813 684 611 302 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI