Restriction map for
pFN21AB9870
(Linear) (Six-base) MAPSORT of: KIBB9870.seq Check: 9232 from: 1 to: 1431
KIBB9870.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 218 1431
Size: 218 1213
AatII G_ACGT'C
Cuts at: 0 344 1028 1330 1431
Size: 344 684 302 101
Fragments arranged by size:
684 344 302 101
AleI CACnn'nnGTG
Cuts at: 0 1147 1431
Size: 1147 284
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 1431
Size: 637 32 762
Fragments arranged by size:
762 637 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 1116 1431
Size: 60 1056 315
Fragments arranged by size:
1056 315 60
ApaI G_GGCC'C
Cuts at: 0 113 1431
Size: 113 1318
AvaI C'yCGr_G
Cuts at: 0 33 1431
Size: 33 1398
BanI G'GyrC_C
Cuts at: 0 22 96 1431
Size: 22 74 1335
Fragments arranged by size:
1335 74 22
BanII G_rGCy'C
Cuts at: 0 88 113 629 1178 1431
Size: 88 25 516 549 253
Fragments arranged by size:
549 516 253 88 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 225 903 1020 1194 1431
Size: 225 678 117 174 237
Fragments arranged by size:
678 237 225 174 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 619 653 1431
Size: 619 34 778
Fragments arranged by size:
778 619 34
BclI T'GATC_A
Cuts at: 0 899 1431
Size: 899 532
BglI GCCn_nnn'nGGC
Cuts at: 0 108 307 1431
Size: 108 199 1124
Fragments arranged by size:
1124 199 108
BglII A'GATC_T
Cuts at: 0 1316 1431
Size: 1316 115
Bme1580I G_kGCm'C
Cuts at: 0 99 113 1431
Size: 99 14 1318
Fragments arranged by size:
1318 99 14
BmgBI CAC'GTC
Cuts at: 0 633 1431
Size: 633 798
BmrI ACTGGGnnnn_n'
Cuts at: 0 115 426 1431
Size: 115 311 1005
Fragments arranged by size:
1005 311 115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1197 1431
Size: 1197 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 639 805 1431
Size: 411 228 166 626
Fragments arranged by size:
626 411 228 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1431
Size: 868 563
BsaI GGTCTCn'nnnn_
Cuts at: 0 865 1006 1431
Size: 865 141 425
Fragments arranged by size:
865 425 141
BsaHI Gr'CG_yC
Cuts at: 0 341 601 1025 1327 1431
Size: 341 260 424 302 104
Fragments arranged by size:
424 341 302 260 104
BsaWI w'CCGG_w
Cuts at: 0 522 1431
Size: 522 909
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 1120 1150 1431
Size: 637 30 453 30 281
Fragments arranged by size:
637 453 281 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 1044 1300 1303 1431
Size: 625 419 256 3 128
Fragments arranged by size:
625 419 256 128 3
BseYI C'CCAG_C
Cuts at: 0 165 1043 1109 1116 1431
Size: 165 878 66 7 315
Fragments arranged by size:
878 315 165 66 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1143 1431
Size: 781 362 288
Fragments arranged by size:
781 362 288
BsiEI CG_ry'CG
Cuts at: 0 992 1305 1431
Size: 992 313 126
Fragments arranged by size:
992 313 126
BsiHKAI G_wGCw'C
Cuts at: 0 370 803 1178 1431
Size: 370 433 375 253
Fragments arranged by size:
433 375 370 253
BsmI GAATG_Cn'
Cuts at: 0 642 1431
Size: 642 789
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1147 1431
Size: 1147 284
Bsp1286I G_dGCh'C
Cuts at: 0 88 99 113 370 629 803 1178 1431
Size: 88 11 14 257 259 174 375 253
Fragments arranged by size:
375 259 257 253 174 88 14 11
BspHI T'CATG_A
Cuts at: 0 719 1431
Size: 719 712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 584 1431
Size: 218 366 847
Fragments arranged by size:
847 366 218
BsrDI GCAATG_nn'
Cuts at: 0 1176 1431
Size: 1176 255
BsrFI r'CCGG_y
Cuts at: 0 310 991 1146 1431
Size: 310 681 155 285
Fragments arranged by size:
681 310 285 155
BssHII G'CGCG_C
Cuts at: 0 213 833 852 1431
Size: 213 620 19 579
Fragments arranged by size:
620 579 213 19
BstEII G'GTnAC_C
Cuts at: 0 123 1339 1431
Size: 123 1216 92
Fragments arranged by size:
1216 123 92
BstYI r'GATC_y
Cuts at: 0 764 1316 1431
Size: 764 552 115
Fragments arranged by size:
764 552 115
BtgI C'CryG_G
Cuts at: 0 77 1431
Size: 77 1354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 735 1431
Size: 735 696
BtsI GCAGTG_nn'
Cuts at: 0 371 972 1431
Size: 371 601 459
Fragments arranged by size:
601 459 371
DrdI GACnn_nn'nnGTC
Cuts at: 0 1030 1431
Size: 1030 401
EaeI y'GGCC_r
Cuts at: 0 248 308 590 989 1431
Size: 248 60 282 399 442
Fragments arranged by size:
442 399 282 248 60
EagI C'GGCC_G
Cuts at: 0 989 1431
Size: 989 442
EarI CTCTTCn'nnn_
Cuts at: 0 687 1431
Size: 687 744
EcoICRI GAG'CTC
Cuts at: 0 1176 1431
Size: 1176 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 639 805 1431
Size: 411 228 166 626
Fragments arranged by size:
626 411 228 166
EcoO109I rG'GnC_Cy
Cuts at: 0 109 110 431 982 1294 1431
Size: 109 1 321 551 312 137
Fragments arranged by size:
551 321 312 137 109 1
FspI TGC'GCA
Cuts at: 0 186 1431
Size: 186 1245
HaeII r_GCGC'y
Cuts at: 0 274 1431
Size: 274 1157
MscI TGG'CCA
Cuts at: 0 592 1431
Size: 592 839
MslI CAynn'nnrTG
Cuts at: 0 403 724 1147 1234 1431
Size: 403 321 423 87 197
Fragments arranged by size:
423 403 321 197 87
MspA1I CmG'CkG
Cuts at: 0 60 1113 1222 1431
Size: 60 1053 109 209
Fragments arranged by size:
1053 209 109 60
NaeI GCC'GGC
Cuts at: 0 993 1431
Size: 993 438
NgoMIV G'CCGG_C
Cuts at: 0 991 1431
Size: 991 440
NspI r_CATG'y
Cuts at: 0 1311 1431
Size: 1311 120
PasI CC'CwG_GG
Cuts at: 0 605 1431
Size: 605 826
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 1116 1431
Size: 60 1056 315
Fragments arranged by size:
1056 315 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 1431
Size: 637 32 762
Fragments arranged by size:
762 637 32
PpuMI rG'GwC_Cy
Cuts at: 0 1294 1431
Size: 1294 137
PshAI GACnn'nnGTC
Cuts at: 0 229 1431
Size: 229 1202
PspOMI G'GGCC_C
Cuts at: 0 109 1431
Size: 109 1322
PstI C_TGCA'G
Cuts at: 0 967 1431
Size: 967 464
PvuI CG_AT'CG
Cuts at: 0 1305 1431
Size: 1305 126
PvuII CAG'CTG
Cuts at: 0 60 1113 1222 1431
Size: 60 1053 109 209
Fragments arranged by size:
1053 209 109 60
SacI G_AGCT'C
Cuts at: 0 1178 1431
Size: 1178 253
SfcI C'TryA_G
Cuts at: 0 963 1431
Size: 963 468
SgrAI Cr'CCGG_yG
Cuts at: 0 1146 1431
Size: 1146 285
SmaI CCC'GGG
Cuts at: 0 35 1431
Size: 35 1396
SmlI C'TyrA_G
Cuts at: 0 883 1431
Size: 883 548
StyI C'CwwG_G
Cuts at: 0 470 875 1431
Size: 470 405 556
Fragments arranged by size:
556 470 405
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 1431
Size: 336 1095
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1431
Size: 1120 32 279
Fragments arranged by size:
1120 279 32
Tth111I GACn'n_nGTC
Cuts at: 0 498 891 1198 1431
Size: 498 393 307 233
Fragments arranged by size:
498 393 307 233
XmaI C'CCGG_G
Cuts at: 0 33 1431
Size: 33 1398
XmnI GAAnn'nnTTC
Cuts at: 0 1423 1431
Size: 1423 8
ZraI GAC'GTC
Cuts at: 0 342 1026 1328 1431
Size: 342 684 302 103
Fragments arranged by size:
684 342 302 103
Enzymes that do cut:
AarI AatII AleI AloI AlwNI ApaI AvaI BanI
BanII BbsI BcgI BclI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BssHII BstEII BstYI BtgI
BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I FspI HaeII MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII SacI SfcI SgrAI SmaI SmlI StyI
TaqII TstI Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BlpI BmtI
Bpu10I BsaAI BsaBI BsiWI BspEI BsrBI BsrGI BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI
DraIII EciI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PsiI PspXI PsrI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI XbaI XcmI XhoI