Restriction map for pFN21AB6950
 (Circular) (Six-base) MAPSORT of: pFN21AB6950.seq  Check: 9356  from: 1  to: 5407



pFN21AB6950  5407 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:42  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3217     278 
   Size:         53      83     186     936    1681    2468

  Fragments arranged by size: 

                2468    1681     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2750    1937 
   Size:        813    4594
 
Acc65I G'GTAC_C

Cuts at:   2728    2728 
   Size:       5407
 
AclI AA'CG_TT

Cuts at:   1563    3534    3907    1563 
   Size:       1971     373    3063

  Fragments arranged by size: 

                3063    1971     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2697    3470    4854    5122    1632 
   Size:       1065     773    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1065     773     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5210
 
AflIII A'CryG_T

Cuts at:   1203    4306    5283    1203 
   Size:       3103     977    1327

  Fragments arranged by size: 

                3103    1327     977
 
AgeI A'CCGG_T

Cuts at:   4238    4238 
   Size:       5407
 
AhdI GACnn_n'nnGTC

Cuts at:   4136    4136 
   Size:       5407
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5407
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5407
 
AlwNI CAG_nnn'CTG

Cuts at:   3150    4722    3150 
   Size:       1572    3835
 
ApaI G_GGCC'C

Cuts at:   2246    2246 
   Size:       5407
 
ApaLI G'TGCA_C

Cuts at:   2264    3464    4620    2264 
   Size:       1200    1156    3051

  Fragments arranged by size: 

                3051    1200    1156
 
ApoI r'AATT_y

Cuts at:   1493    2716    2942    3094    5203    1493 
   Size:       1223     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1223     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3961     160 
   Size:        872    2929    1606

  Fragments arranged by size: 

                2929    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5407
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2732    1870 
   Size:         73     789    4545

  Fragments arranged by size: 

                4545     789      73
 
BamHI G'GATC_C

Cuts at:   2737    3104    2737 
   Size:        367    5040
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2728    4183     618 
   Size:        325     217     627     941    1455    1842

  Fragments arranged by size: 

                1842    1455     941     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2104    2246    2275    2726    1391 
   Size:        713     142      29     451    4072

  Fragments arranged by size: 

                4072     713     451     142      29
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2522     928 
   Size:        971     623    3813

  Fragments arranged by size: 

                3813     971     623
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2255    2289    3598    3632    1903 
   Size:         34     318      34    1309      34    3678

  Fragments arranged by size: 

                3678    1309     318      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3299    4509    3299 
   Size:       1210    4197
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5407
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4018     136 
   Size:        107     122      71     631     605    2346    1525

  Fragments arranged by size: 

                2346    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2545    5402    2545 
   Size:       2857    2550
 
BlpI GC'TnA_GC

Cuts at:   2328    2824    2328 
   Size:        496    4911
 
Bme1580I G_kGCm'C

Cuts at:   1790    2246    2268    3468    4624    1790 
   Size:        456      22    1200    1156    2573

  Fragments arranged by size: 

                2573    1200    1156     456      22
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5407
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3138    4096     442 
   Size:        416      53      83     399      24    1721     958    1753

  Fragments arranged by size: 

                1753    1721     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5407
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2685    2685 
   Size:       5407
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2115    2481    4067    1239 
   Size:        876     366    1586    2579

  Fragments arranged by size: 

                2579    1586     876     366
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2546    3532    4397    4695    2546 
   Size:        986     865     298    3258

  Fragments arranged by size: 

                3258     986     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4070     882 
   Size:        617    2571    2219

  Fragments arranged by size: 

                2571    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3198     493 
   Size:        711    1994    2702

  Fragments arranged by size: 

                2702    1994     711
 
BsaBI GATnn'nnATC

Cuts at:   5401    5401 
   Size:       5407
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3214    3596    4231     275 
   Size:         53      83     186     936    1681     382     635    1451

  Fragments arranged by size: 

                1681    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3840    4238    4512    4659    5070    3840 
   Size:        398     274     147     411    4177

  Fragments arranged by size: 

                4177     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2003    2033    1178 
   Size:         30     165      30     600      30    4552

  Fragments arranged by size: 

                4552     600     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2112    1186 
   Size:        926    4481
 
BseYI C'CCAG_C

Cuts at:   4610    5106    4610 
   Size:        496    4911
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4834
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2857    3618    4646    1540 
   Size:        275     186     856     761    1028    2301

  Fragments arranged by size: 

                2301    1028     856     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2268    2275    3468    3553    4624    2268 
   Size:          7    1193      85    1071    3051

  Fragments arranged by size: 

                3051    1193    1071      85       7
 
BsmI GAATG_Cn'

Cuts at:   2918    3011    2918 
   Size:         93    5314
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2104    2246    2268    2275    2726    3468    3553 
   Size:        399     314     142      22       7     451     742      85

Cuts at:   3553    4624    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     451     399     314     142      85
                  22       7
 
BspEI T'CCGG_A

Cuts at:   5070    5070 
   Size:       5407
 
BspHI T'CATG_A

Cuts at:   3296    5026    3296 
   Size:       1730    3677
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2762     844 
   Size:       1918    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3294    1394 
   Size:        429    1471    3507

  Fragments arranged by size: 

                3507    1471     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2496    2650    3902    4076      65 
   Size:       1183    1248     154    1252     174    1396

  Fragments arranged by size: 

                1396    1252    1248    1183     174     154
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4051    4238    1700 
   Size:        110    2241     187    2869

  Fragments arranged by size: 

                2869    2241     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2188    2229      96 
   Size:       2092      41    3274

  Fragments arranged by size: 

                3274    2092      41
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5407
 
BssSI C'ACGA_G

Cuts at:   3467    4479    3467 
   Size:       1012    4395
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5407
 
BstBI TT'CG_AA

Cuts at:   4211    4211 
   Size:       5407
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5407
 
BstYI r'GATC_y

Cuts at:   1972    2452    2545    2737    3104    3489    3506    4958    5044 
   Size:        480      93     192     367     385      17    1452      86

Cuts at:   5044    5056    5402    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     480     385     367     346     192      93
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2109     513 
   Size:        552     939     105    3811

  Fragments arranged by size: 

                3811     939     552     105
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4668

  Fragments arranged by size: 

                4668     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2923    3717    3737    2923 
   Size:        794      20    4593

  Fragments arranged by size: 

                4593     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5407
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2115    2150     529 
   Size:         35    1551      35    3786

  Fragments arranged by size: 

                3786    1551      35      35
 
DraI TTT'AAA

Cuts at:   2711    2711 
   Size:       5407
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4414     809 
   Size:        507    3098    1802

  Fragments arranged by size: 

                3098    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2854    3743    5372 
   Size:         54    1425      50     275      18    1024     889    1629

Cuts at:   5372       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1024     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2854    1537 
   Size:        275    1042    4090

  Fragments arranged by size: 

                4090    1042     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3337    1353 
   Size:       1984    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2024    3994    4368    4514     387 
   Size:       1064     138     435    1970     374     146    1280

  Fragments arranged by size: 

                1970    1280    1064     435     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2273    2273 
   Size:       5407
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2115    2481    2697    3470    4067    4854    5122 
   Size:        393     483     366     216     773     597     787     268

Cuts at:   5122    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     787     773     597     483     393     366     268
                 216
 
EcoO109I rG'GnC_Cy

Cuts at:   2243    2243 
   Size:       5407
 
EcoRI G'AATT_C

Cuts at:   2716    3094    2716 
   Size:        378    5029
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2614    2707    2614 
   Size:         93    5314
 
FspI TGC'GCA

Cuts at:   3913    3913 
   Size:       5407
 
HaeII r_GCGC'y

Cuts at:   1678    4554    1678 
   Size:       2876    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4048    4080    4122    4154    1373 
   Size:         32     135      32    2476      32      42      32    2626

  Fragments arranged by size: 

                2626    2476     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2751    2997     669 
   Size:        988     281     813     246    3079

  Fragments arranged by size: 

                3079     988     813     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2596     748 
   Size:       1848    3559
 
HpaI GTT'AAC

Cuts at:   2997    2997 
   Size:       5407
 
KpnI G_GTAC'C

Cuts at:   2732    2732 
   Size:       5407
 
MfeI C'AATT_G

Cuts at:   3006    3006 
   Size:       5407
 
MluI A'CGCG_T

Cuts at:   5283    5283 
   Size:       5407
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2399    2614    2696    4521    4705    1305 
   Size:       1094     215      82    1825     184    2007

  Fragments arranged by size: 

                2007    1825    1094     215     184      82
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3585

  Fragments arranged by size: 

                3585    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3365    3724    3883     518 
   Size:        648    2199     359     159    2042

  Fragments arranged by size: 

                2199    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2332    2823    3500    4648    4893    1606 
   Size:        726     491     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     726     677     491     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3916

  Fragments arranged by size: 

                3916     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5407
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5407
 
NotI GC'GGCC_GC

Cuts at:   2854    2854 
   Size:       5407
 
NspI r_CATG'y

Cuts at:   1132    1229    2765    4310    1132 
   Size:         97    1536    1545    2229

  Fragments arranged by size: 

                2229    1545    1536      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5407
 
PciI A'CATG_T

Cuts at:   4306    4306 
   Size:       5407
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5407
 
PmeI GTTT'AAAC

Cuts at:   2711    2711 
   Size:       5407
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3476    3508    4989    5021    3476 
   Size:         32    1481      32    3862

  Fragments arranged by size: 

                3862    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5407
 
PsiI TTA'TAA

Cuts at:   2977    2977 
   Size:       5407
 
PspOMI G'GGCC_C

Cuts at:   2242    2242 
   Size:       5407
 
PstI C_TGCA'G

Cuts at:    830    2759     830 
   Size:       1929    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5407
 
PvuII CAG'CTG

Cuts at:   2332    2332 
   Size:       5407
 
SacI G_AGCT'C

Cuts at:   2275    2275 
   Size:       5407
 
SalI G'TCGA_C

Cuts at:   1936    2749    1936 
   Size:        813    4594
 
SbfI CC_TGCA'GG

Cuts at:   2759    2759 
   Size:       5407
 
ScaI AGT'ACT

Cuts at:   3655    5367    3655 
   Size:       1712    3695
 
SfcI C'TryA_G

Cuts at:    826    1045    2088    2322    2484    2755    3890    4571    4762 
   Size:        219    1043     234     162     271    1135     681     191

Cuts at:   4762     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1043     681     271     234     219     191
                 162
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5407
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5407
 
SmaI CCC'GGG

Cuts at:   1872    2734    1872 
   Size:        862    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2525    3511    4412    4674     820 
   Size:        197     926     582     986     901     262    1553

  Fragments arranged by size: 

                1553     986     926     901     582     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3198     493 
   Size:       2705    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5407
 
SphI G_CATG'C

Cuts at:   1132    2765    1132 
   Size:       1633    3774
 
SspI AAT'ATT

Cuts at:      5      52    3331       5 
   Size:         47    3279    2081

  Fragments arranged by size: 

                3279    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5407
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2846     513 
   Size:        552     939     842    3074

  Fragments arranged by size: 

                3074     939     842     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3434    3451    3604    3789    4234     325 
   Size:       1255    1854      17     153     185     445    1498

  Fragments arranged by size: 

                1854    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2188    2229    3653 
   Size:        274      80      33      51    1443     211      41    1424

Cuts at:   3653    5365      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1424     274     211     138      80      51
                  41      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5367    5399    5367 
   Size:         32    5375
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5407
 
XbaI T'CTAG_A

Cuts at:   2743    2743 
   Size:       5407
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5407
 
XmaI C'CCGG_G

Cuts at:   1870    2732    1870 
   Size:        862    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2316    2519    3536    2316 
   Size:        203    1017    4187

  Fragments arranged by size: 

                4187    1017     203
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3215     276 
   Size:         53      83     186     936    1681    2468

  Fragments arranged by size: 

                2468    1681     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI
     PvuI    PvuII     SacI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI
    RsrII    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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