(Circular) (Six-base) MAPSORT of: pFN21AB6950.seq Check: 9356 from: 1 to: 5407 pFN21AB6950 5407 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 3470 4854 5122 1632 Size: 1065 773 1384 268 1917 Fragments arranged by size: 1917 1384 1065 773 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5407 AlwNI CAG_nnn'CTG Cuts at: 3150 4722 3150 Size: 1572 3835 ApaI G_GGCC'C Cuts at: 2246 2246 Size: 5407 ApaLI G'TGCA_C Cuts at: 2264 3464 4620 2264 Size: 1200 1156 3051 Fragments arranged by size: 3051 1200 1156 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2732 1870 Size: 73 789 4545 Fragments arranged by size: 4545 789 73 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2728 4183 618 Size: 325 217 627 941 1455 1842 Fragments arranged by size: 1842 1455 941 627 325 217 BanII G_rGCy'C Cuts at: 1391 2104 2246 2275 2726 1391 Size: 713 142 29 451 4072 Fragments arranged by size: 4072 713 451 142 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2522 928 Size: 971 623 3813 Fragments arranged by size: 3813 971 623 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2255 2289 3598 3632 1903 Size: 34 318 34 1309 34 3678 Fragments arranged by size: 3678 1309 318 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4018 136 Size: 107 122 71 631 605 2346 1525 Fragments arranged by size: 2346 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2545 5402 2545 Size: 2857 2550 BlpI GC'TnA_GC Cuts at: 2328 2824 2328 Size: 496 4911 Bme1580I G_kGCm'C Cuts at: 1790 2246 2268 3468 4624 1790 Size: 456 22 1200 1156 2573 Fragments arranged by size: 2573 1200 1156 456 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 4096 442 Size: 416 53 83 399 24 1721 958 1753 Fragments arranged by size: 1753 1721 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2685 2685 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2481 4067 1239 Size: 876 366 1586 2579 Fragments arranged by size: 2579 1586 876 366 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2546 3532 4397 4695 2546 Size: 986 865 298 3258 Fragments arranged by size: 3258 986 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4070 882 Size: 617 2571 2219 Fragments arranged by size: 2571 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3214 3596 4231 275 Size: 53 83 186 936 1681 382 635 1451 Fragments arranged by size: 1681 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3840 4238 4512 4659 5070 3840 Size: 398 274 147 411 4177 Fragments arranged by size: 4177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 1178 Size: 30 165 30 600 30 4552 Fragments arranged by size: 4552 600 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2112 1186 Size: 926 4481 BseYI C'CCAG_C Cuts at: 4610 5106 4610 Size: 496 4911 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4834 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2857 3618 4646 1540 Size: 275 186 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 2275 3468 3553 4624 2268 Size: 7 1193 85 1071 3051 Fragments arranged by size: 3051 1193 1071 85 7 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2246 2268 2275 2726 3468 3553 Size: 399 314 142 22 7 451 742 85 Cuts at: 3553 4624 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 451 399 314 142 85 22 7 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2762 844 Size: 1918 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3294 1394 Size: 429 1471 3507 Fragments arranged by size: 3507 1471 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2496 2650 3902 4076 65 Size: 1183 1248 154 1252 174 1396 Fragments arranged by size: 1396 1252 1248 1183 174 154 BsrFI r'CCGG_y Cuts at: 1700 1810 4051 4238 1700 Size: 110 2241 187 2869 Fragments arranged by size: 2869 2241 187 110 BsrGI T'GTAC_A Cuts at: 96 2188 2229 96 Size: 2092 41 3274 Fragments arranged by size: 3274 2092 41 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5407 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5407 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2452 2545 2737 3104 3489 3506 4958 5044 Size: 480 93 192 367 385 17 1452 86 Cuts at: 5044 5056 5402 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 480 385 367 346 192 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 513 Size: 552 939 105 3811 Fragments arranged by size: 3811 939 552 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3717 3737 2923 Size: 794 20 4593 Fragments arranged by size: 4593 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2115 2150 529 Size: 35 1551 35 3786 Fragments arranged by size: 3786 1551 35 35 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4414 809 Size: 507 3098 1802 Fragments arranged by size: 3098 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2854 3743 5372 Size: 54 1425 50 275 18 1024 889 1629 Cuts at: 5372 8 Size: 43 Fragments arranged by size: 1629 1425 1024 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2854 1537 Size: 275 1042 4090 Fragments arranged by size: 4090 1042 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3337 1353 Size: 1984 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3994 4368 4514 387 Size: 1064 138 435 1970 374 146 1280 Fragments arranged by size: 1970 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2273 2273 Size: 5407 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2481 2697 3470 4067 4854 5122 Size: 393 483 366 216 773 597 787 268 Cuts at: 5122 1239 Size: 1524 Fragments arranged by size: 1524 787 773 597 483 393 366 268 216 EcoO109I rG'GnC_Cy Cuts at: 2243 2243 Size: 5407 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2707 2614 Size: 93 5314 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 4554 1678 Size: 2876 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373 Size: 32 135 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 2997 669 Size: 988 281 813 246 3079 Fragments arranged by size: 3079 988 813 281 246 HindIII A'AGCT_T Cuts at: 748 2596 748 Size: 1848 3559 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2399 2614 2696 4521 4705 1305 Size: 1094 215 82 1825 184 2007 Fragments arranged by size: 2007 1825 1094 215 184 82 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3585 Fragments arranged by size: 3585 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3365 3724 3883 518 Size: 648 2199 359 159 2042 Fragments arranged by size: 2199 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2332 2823 3500 4648 4893 1606 Size: 726 491 677 1148 245 2120 Fragments arranged by size: 2120 1148 726 677 491 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3916 Fragments arranged by size: 3916 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2765 4310 1132 Size: 97 1536 1545 2229 Fragments arranged by size: 2229 1545 1536 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5407 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5407 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4989 5021 3476 Size: 32 1481 32 3862 Fragments arranged by size: 3862 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PspOMI G'GGCC_C Cuts at: 2242 2242 Size: 5407 PstI C_TGCA'G Cuts at: 830 2759 830 Size: 1929 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 PvuII CAG'CTG Cuts at: 2332 2332 Size: 5407 SacI G_AGCT'C Cuts at: 2275 2275 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2088 2322 2484 2755 3890 4571 4762 Size: 219 1043 234 162 271 1135 681 191 Cuts at: 4762 826 Size: 1471 Fragments arranged by size: 1471 1135 1043 681 271 234 219 191 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2734 1872 Size: 862 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2525 3511 4412 4674 820 Size: 197 926 582 986 901 262 1553 Fragments arranged by size: 1553 986 926 901 582 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5407 StyI C'CwwG_G Cuts at: 513 1065 2004 2846 513 Size: 552 939 842 3074 Fragments arranged by size: 3074 939 842 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3434 3451 3604 3789 4234 325 Size: 1255 1854 17 153 185 445 1498 Fragments arranged by size: 1854 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2188 2229 3653 Size: 274 80 33 51 1443 211 41 1424 Cuts at: 3653 5365 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1424 274 211 138 80 51 41 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2732 1870 Size: 862 4545 XmnI GAAnn'nnTTC Cuts at: 2316 2519 3536 2316 Size: 203 1017 4187 Fragments arranged by size: 4187 1017 203 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI