Restriction map for
pFN21AB6949
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AvaII
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HapII Hin1I HincII
HindII HindIII HpaI HpaII KasI
MaeII MamI MfeI MflI MluI
MroI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Van91I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 581 GGTACC G^GTACC
AlwI 456 GATCC GGATC(4/5)
Asp718I 581 GGTACC G^GTACC
AspI 157 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 239 CTCGAG C^[CT]CG[GA]G
BanI 581 GGTACC G^G[CT][GA]CC
BbvI 188 GCTGC GCAGC(8/12)
BcgI 231 GCAGGCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 520 CTAG C^TAG
BglI 226 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 255 GGTCTC GGTCTC(1/5)
BsgI 69 CTGCAC GTGCAG(16/14)
BsmBI 326 GAGACG CGTCTC(1/5)
BsoBI 239 CTCGAG C^[CT]CG[GA]G
Bsp1407I 137 TGTACA T^GTACA
BspMI 385 GCAGGT ACCTGC(4/8)
BsrBI 594 CCGCTC CCGCTC(-3/-3)
BsrGI 137 TGTACA T^GTACA
BstXI 281 CCATGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 315 GCGC GCG^C
DdeI 605 CTAAG C^T[ACGT]AG
DpnII 456 GATC ^GATC
EarI 615 CTCTTC CTCTTC(1/4)
HgaI 161 GCGTC GACGC(5/10)
HhaI 315 GCGC GCG^C
HinP1I 315 GCGC G^CGC
HinfI 202 GACTC G^A[ACGT]TC
HphI 109 GGTGA GGTGA(8/7)
KpnI 581 GGTACC GGTAC^C
Ksp632I 615 CTCTTC CTCTTC(1/4)
KspI 221 CCGCGG CCGC^GG
MaeI 520 CTAG C^TAG
MboI 456 GATC ^GATC
MseI 470 TTAA T^TAA
MspA1I 221 CCGCGG C[AC]G^C[GT]G
NdeII 456 GATC ^GATC
NlaIV 581 GGTACC GG[ACGT]^[ACGT]CC
PaeR7I 239 CTCGAG C^TCGAG
PflFI 157 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 202 GACTC GAGTC(4/5)
PstI 16 CTGCAG CTGCA^G
SacI 96 GAGCTC GAGCT^C
SacII 221 CCGCGG CCGC^GG
Sau3AI 456 GATC ^GATC
SfcI 16 CTGCAG C^T[GA][CT]AG
SfiI 225 GGCCTTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 239 CTCGAG C^T[CT][GA]AG
SspBI 137 TGTACA T^GTACA
TaqI 240 TCGA T^CGA
Tru9I 470 TTAA T^TAA
TseI 188 GCTGC G^C[AT]GC
Tth111I 157 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 240 TCGA T^CGA
XcmI 35 CCATGGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 239 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AccII 222 CGCG CG^CG
314 CGCG
AlwNI 19 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
509 CAGGGTCTG
BanII 96 GAGCTC G[GA]GC[CT]^C
242 GAGCCC
BsmAI 326 GAGAC GTCTC(1/5)
256 GTCTC
BstUI 222 CGCG CG^CG
314 CGCG
MboII 339 GAAGA GAAGA(8/7)
616 TCTTC
MnlI 365 GAGG CCTC(7/6)
533 GAGG
MslI 174 CACGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
282 CATGTTTGTG
MvnI 222 CGCG CG^CG
314 CGCG
NcoI 35 CCATGG C^CATGG
356 CCATGG
Tsp509I 7 AATT ^AATT
372 AATT
TspRI 85 CAGTG CA[GC]TG[ACGT][ACGT]^
539 CACTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI Alw44I ApaLI
AspHI BalI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII ItaI MaeIII MluNI
MscI MvaI MwoI NlaIII RsaI
Sau96I ScrFI StyI Tsp45I