Restriction map for pFN21AB6949
 (Circular) (Six-base) MAPSORT of: pFN21AB6949.seq  Check: 8553  from: 1  to: 5338



pFN21AB6949  5338 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:42  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3148     278 
   Size:         53      83     186     936    1612    2468

  Fragments arranged by size: 

                2468    1612     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2681    1937 
   Size:        744    4594
 
Acc65I G'GTAC_C

Cuts at:   2586    2659    2586 
   Size:         73    5265
 
AclI AA'CG_TT

Cuts at:   1563    3465    3838    1563 
   Size:       1902     373    3063

  Fragments arranged by size: 

                3063    1902     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3401    4785    5053    1632 
   Size:       1769    1384     268    1917

  Fragments arranged by size: 

                1917    1769    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5141
 
AflIII A'CryG_T

Cuts at:   1203    4237    5214    1203 
   Size:       3034     977    1327

  Fragments arranged by size: 

                3034    1327     977
 
AgeI A'CCGG_T

Cuts at:   4169    4169 
   Size:       5338
 
AhdI GACnn_n'nnGTC

Cuts at:   4067    4067 
   Size:       5338
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5338
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5338
 
AlwNI CAG_nnn'CTG

Cuts at:   2029    2519    3081    4653    2029 
   Size:        490     562    1572    2714

  Fragments arranged by size: 

                2714    1572     562     490
 
ApaLI G'TGCA_C

Cuts at:   2314    2541    2592    3395    4551    2314 
   Size:        227      51     803    1156    3101

  Fragments arranged by size: 

                3101    1156     803     227      51
 
ApoI r'AATT_y

Cuts at:   1493    2647    2873    3025    5134    1493 
   Size:       1154     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1154     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3892     160 
   Size:        872    2860    1606

  Fragments arranged by size: 

                2860    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5338
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2244    2663    1870 
   Size:         73     301     419    4545

  Fragments arranged by size: 

                4545     419     301      73
 
BamHI G'GATC_C

Cuts at:   2668    3035    2668 
   Size:        367    4971
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2586    2659    4114     618 
   Size:        325     217     627     799      73    1455    1842

  Fragments arranged by size: 

                1842    1455     799     627     325     217      73
 
BanII G_rGCy'C

Cuts at:   1391    2105    2251    2657    1391 
   Size:        714     146     406    4072

  Fragments arranged by size: 

                4072     714     406     146
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4367
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2225    2259    3529    3563    1903 
   Size:         34     288      34    1270      34    3678

  Fragments arranged by size: 

                3678    1270     288      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3230    4440    3230 
   Size:       1210    4128
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5338
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2237    3949     136 
   Size:        107     122      71     631     605     565    1712    1525

  Fragments arranged by size: 

                1712    1525     631     605     565     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5333    5333 
   Size:       5338
 
BlpI GC'TnA_GC

Cuts at:   2755    2755 
   Size:       5338
 
Bme1580I G_kGCm'C

Cuts at:   1790    2318    2545    2596    3399    4555    1790 
   Size:        528     227      51     803    1156    2573

  Fragments arranged by size: 

                2573    1156     803     528     227      51
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5338
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3069    4027     442 
   Size:        416      53      83     399      24    1652     958    1753

  Fragments arranged by size: 

                1753    1652     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3998    1239 
   Size:       2759    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3463    4328    4626    3463 
   Size:        865     298    4175

  Fragments arranged by size: 

                4175     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2266    4001     882 
   Size:        617     767    1735    2219

  Fragments arranged by size: 

                2219    1735     767     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3129     493 
   Size:        711    1925    2702

  Fragments arranged by size: 

                2702    1925     711
 
BsaBI GATnn'nnATC

Cuts at:   5332    5332 
   Size:       5338
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3145    3527    4162     275 
   Size:         53      83     186     936    1612     382     635    1451

  Fragments arranged by size: 

                1612    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3771    4169    4443    4590    5001    3771 
   Size:        398     274     147     411    4108

  Fragments arranged by size: 

                4108     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5113

  Fragments arranged by size: 

                5113     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5338
 
BseYI C'CCAG_C

Cuts at:   2029    2050    2055    2272    2508    2582    4541    5037    2029 
   Size:         21       5     217     236      74    1959     496    2330

  Fragments arranged by size: 

                2330    1959     496     236     217      74      21       5
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2059    1163 
   Size:        573     323    4442

  Fragments arranged by size: 

                4442     573     323
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2788    3549    4577    1540 
   Size:        275     186     787     761    1028    2301

  Fragments arranged by size: 

                2301    1028     787     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2105    2180    2217    2318    2545    2596    3399    3484    4555 
   Size:         75      37     101     227      51     803      85    1071

Cuts at:   4555    2105 
   Size:       2888

  Fragments arranged by size: 

                2888    1071     803     227     101      85      75      51
                  37
 
BsmI GAATG_Cn'

Cuts at:   2849    2942    2849 
   Size:         93    5245
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2325    2325 
   Size:       5338
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2105    2180    2217    2251    2318    2545    2596 
   Size:        399     315      75      37      34      67     227      51

Cuts at:   2596    2657    3399    3484    4555    1391 
   Size:         61     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     315     227      85      75
                  67      61      51      37      34
 
BspEI T'CCGG_A

Cuts at:   5001    5001 
   Size:       5338
 
BspHI T'CATG_A

Cuts at:   3227    4957    3227 
   Size:       1730    3608
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2381    2693     844 
   Size:       1537     312    3489

  Fragments arranged by size: 

                3489    1537     312
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2601    3225    1394 
   Size:        429     778     624    3507

  Fragments arranged by size: 

                3507     778     624     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3833    4007      65 
   Size:       1183    2585     174    1396

  Fragments arranged by size: 

                2585    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3982    4169    1700 
   Size:        110    2172     187    2869

  Fragments arranged by size: 

                2869    2172     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2142      96 
   Size:       2046    3292
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5338
 
BssSI C'ACGA_G

Cuts at:   3398    4410    3398 
   Size:       1012    4326
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2313    2396    2519     794 
   Size:       1519      83     123    3613

  Fragments arranged by size: 

                3613    1519     123      83
 
BstBI TT'CG_AA

Cuts at:   4142    4142 
   Size:       5338
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5338
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2293    2293 
   Size:       5338
 
BstYI r'GATC_y

Cuts at:   1972    2668    3035    3420    3437    4889    4975    4987    5333 
   Size:        696     367     385      17    1452      86      12     346

Cuts at:   5333    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     696     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2040    2226    2361     513 
   Size:        552     939      36     186     135    3490

  Fragments arranged by size: 

                3490     939     552     186     135      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4599

  Fragments arranged by size: 

                4599     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2096    2543    2854    3648    3668    2096 
   Size:        447     311     794      20    3766

  Fragments arranged by size: 

                3766     794     447     311      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5338
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5303
 
DraI TTT'AAA

Cuts at:   2642    2642 
   Size:       5338
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4345     809 
   Size:        507    3029    1802

  Fragments arranged by size: 

                3029    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2007    2283    2295 
   Size:         54    1425      50     275      18     177     276      12

Cuts at:   2295    2425    2785    3674    5303       8 
   Size:        130     360     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     360     276     275     177     130
                  54      50      43      18      12
 
EagI C'GGCC_G

Cuts at:   1537    1812    2785    1537 
   Size:        275     973    4090

  Fragments arranged by size: 

                4090     973     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2626    3268    1353 
   Size:       1273     642    3423

  Fragments arranged by size: 

                3423    1273     642
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3925    4299    4445     387 
   Size:       1064     138    2336     374     146    1280

  Fragments arranged by size: 

                2336    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2103    2103 
   Size:       5338
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3401    3998    4785    5053    1239 
   Size:        393    1769     597     787     268    1524

  Fragments arranged by size: 

                1769    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2647    3025    2647 
   Size:        378    4960
 
FspI TGC'GCA

Cuts at:   3844    3844 
   Size:       5338
 
HaeII r_GCGC'y

Cuts at:   1678    4485    1678 
   Size:       2807    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3979    4011    4053    4085    1373 
   Size:         32     135      32    2407      32      42      32    2626

  Fragments arranged by size: 

                2626    2407     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2682    2928     669 
   Size:        988     281     744     246    3079

  Fragments arranged by size: 

                3079     988     744     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5338
 
HpaI GTT'AAC

Cuts at:   2928    2928 
   Size:       5338
 
KpnI G_GTAC'C

Cuts at:   2590    2663    2590 
   Size:         73    5265
 
MfeI C'AATT_G

Cuts at:   2937    2937 
   Size:       5338
 
MluI A'CGCG_T

Cuts at:   5214    5214 
   Size:       5338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2640    4452    4636    1305 
   Size:       1335    1812     184    2007

  Fragments arranged by size: 

                2007    1812    1335     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2009    2285    2297    2427      10 
   Size:         54    1425     343     177     276      12     130    2921

  Fragments arranged by size: 

                2921    1425     343     276     177     130      54      12
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2183    2291    3296    3655    3814     518 
   Size:        648    1017     108    1005     359     159    2042

  Fragments arranged by size: 

                2042    1017    1005     648     359     159     108
 
MspA1I CmG'CkG

Cuts at:   1606    2228    2754    3431    4579    4824    1606 
   Size:        622     526     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     622     526     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2040    2361     513 
   Size:        552     939      36     321    3490

  Fragments arranged by size: 

                3490     939     552     321      36
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5338
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5338
 
NotI GC'GGCC_GC

Cuts at:   2785    2785 
   Size:       5338
 
NspI r_CATG'y

Cuts at:   1132    1229    2696    4241    1132 
   Size:         97    1467    1545    2229

  Fragments arranged by size: 

                2229    1545    1467      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5338
 
PciI A'CATG_T

Cuts at:   4237    4237 
   Size:       5338
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5338
 
PmeI GTTT'AAAC

Cuts at:   2642    2642 
   Size:       5338
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3407    3439    4920    4952    3407 
   Size:         32    1481      32    3793

  Fragments arranged by size: 

                3793    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5338
 
PsiI TTA'TAA

Cuts at:   2451    2908    2451 
   Size:        457    4881
 
PspXI vC'TCGA_Gb

Cuts at:   2244    2244 
   Size:       5338
 
PstI C_TGCA'G

Cuts at:    830    2025    2690     830 
   Size:       1195     665    3478

  Fragments arranged by size: 

                3478    1195     665
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5338
 
SacI G_AGCT'C

Cuts at:   2105    2105 
   Size:       5338
 
SacII CC_GC'GG

Cuts at:   2229    2229 
   Size:       5338
 
SalI G'TCGA_C

Cuts at:   1936    2680    1936 
   Size:        744    4594
 
SbfI CC_TGCA'GG

Cuts at:   2690    2690 
   Size:       5338
 
ScaI AGT'ACT

Cuts at:   3586    5298    3586 
   Size:       1712    3626
 
SfcI C'TryA_G

Cuts at:    826    1045    2021    2686    3821    4502    4693     826 
   Size:        219     976     665    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135     976     681     665     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2237    2237 
   Size:       5338
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5338
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5338
 
SmaI CCC'GGG

Cuts at:   1872    2665    1872 
   Size:        793    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2244    3442    4343    4605     820 
   Size:        197     926     301    1198     901     262    1553

  Fragments arranged by size: 

                1553    1198     926     901     301     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3129     493 
   Size:       2636    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5338
 
SphI G_CATG'C

Cuts at:   1132    2696    1132 
   Size:       1564    3774
 
SspI AAT'ATT

Cuts at:      5      52    3262       5 
   Size:         47    3210    2081

  Fragments arranged by size: 

                3210    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5338
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2040    2361    2403    2625    2777     513 
   Size:        552     939      36     321      42     222     152    3074

  Fragments arranged by size: 

                3074     939     552     321     222     152      42      36
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2047    2354    3365    3382    3535    3720    4165 
   Size:       1255     467     307    1011      17     153     185     445

Cuts at:   4165     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1011     467     445     307     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2142    3584    5296 
   Size:        274      80      33      51    1443     165    1442    1712

Cuts at:   5296      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1442     274     165     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5298    5330    5298 
   Size:         32    5306
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2165    1316 
   Size:        849    4489
 
XbaI T'CTAG_A

Cuts at:   2674    2674 
   Size:       5338
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2047    2632    2047 
   Size:        585    4753
 
XhoI C'TCGA_G

Cuts at:   1943    2244    1943 
   Size:        301    5037
 
XmaI C'CCGG_G

Cuts at:   1870    2663    1870 
   Size:        793    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3467    3467 
   Size:       5338
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3146     276 
   Size:         53      83     186     936    1612    2468

  Fragments arranged by size: 

                2468    1612     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI    PspXI     PstI     PvuI     SacI
    SacII     SalI     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI  BstZ17I   Bsu36I   DraIII
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI     PsrI    PvuII    RsrII    SanDI     SapI    SexAI     SfoI
     SrfI     SwaI
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