Restriction map for
pFN21AB6944
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI TseI Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflIII 566 ACATGT A^C[GA][CT]GT
Alw44I 535 GTGCAC G^TGCAC
ApaI 503 GGGCCC GGGCC^C
ApaLI 535 GTGCAC G^TGCAC
AvaII 144 GGTCC G^G[AT]CC
BalI 492 TGGCCA TGG^CCA
BbsI 124 GTCTTC GAAGAC(2/6)
BfaI 514 CTAG C^TAG
BglI 590 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 167 AGATCT A^GATCT
BpuAI 124 GTCTTC GAAGAC(2/6)
BspHI 561 TCATGA T^CATGA
BspLU11I 566 ACATGT A^CATGT
BsrFI 36 GCCGGT [GA]^CCGG[CT]
BstAPI 624 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 281 CCAAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 167 AGATCT [GA]^GATC[CT]
Cfr10I 36 GCCGGT [GA]^CCGG[CT]
DpnII 168 GATC ^GATC
DrdI 136 GACTCCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 315 CCACGG C^C[GA][CT]GG
EaeI 492 TGGCCA [CT]^GGCC[GA]
EarI 181 CTCTTC CTCTTC(1/4)
FauI 685 CCCGC CCCGC(4/6)
HaeII 62 GGCGCT [GA]GCGC^[CT]
HgaI 639 GCGTC GACGC(5/10)
HinfI 136 GACTC G^A[ACGT]TC
Ksp632I 181 CTCTTC CTCTTC(1/4)
MaeI 514 CTAG C^TAG
MaeII 47 ACGT A^CGT
MboI 168 GATC ^GATC
MflI 167 AGATCT [GA]^GATC[CT]
MluNI 492 TGGCCA TGG^CCA
MscI 492 TGGCCA TGG^CCA
MseI 203 TTAA T^TAA
NdeII 168 GATC ^GATC
PleI 136 GACTC GAGTC(4/5)
RcaI 561 TCATGA T^CATGA
SacI 178 GAGCTC GAGCT^C
SapI 180 GCTCTTC GCTCTTC(1/4)
Sau3AI 168 GATC ^GATC
SgrAI 35 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 392 CCCGGG CCC^GGG
SphI 195 GCATGC GCATG^C
Tru9I 203 TTAA T^TAA
XhoII 167 AGATCT [GA]^GATC[CT]
XmaCI 392 CCCGGG C^CCGGG
XmaI 392 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 175 CGCG CG^CG
419 CGCG
AvaI 392 CCCGGG C^[CT]CG[GA]G
500 CTCGGG
BanI 304 GGCACC G^G[CT][GA]CC
547 GGCACC
BpmI 250 CTGGAG CTGGAG(16/14)
679 CTGGAG
BseRI 188 CTCCTC GAGGAG(10/8)
10 GAGGAG
BsmFI 134 GGGAC GGGAC(10/14)
72 GTCCC
BsoBI 392 CCCGGG C^[CT]CG[GA]G
500 CTCGGG
BspMI 578 ACCTGC ACCTGC(4/8)
333 GCAGGT
BstUI 175 CGCG CG^CG
419 CGCG
CfoI 63 GCGC GCG^C
174 GCGC
DdeI 540 CTCAG C^T[ACGT]AG
586 CTGAG
EcoT14I 140 CCTTGG C^C[AT][AT]GG
663 CCAAGG
HhaI 63 GCGC GCG^C
174 GCGC
HinP1I 63 GCGC G^CGC
174 GCGC
MaeIII 221 GTGAC ^GT[ACGT]AC
337 GTGAC
MslI 33 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
538 CACTCAGATG
MvnI 175 CGCG CG^CG
419 CGCG
StyI 140 CCTTGG C^C[AT][AT]GG
663 CCAAGG
Tsp45I 221 GTGAC ^GT[GC]AC
337 GTGAC
TspRI 160 CAGTG CA[GC]TG[ACGT][ACGT]^
485 CACTG
XcmI 281 CCAAGATTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
356 CCATTGTGCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BcnI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
NspI RsaI Sau96I ScrFI SfaNI