(Circular) (Six-base) MAPSORT of: pFN21AB6944.seq Check: 9642 from: 1 to: 5398 pFN21AB6944 5398 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2329 2329 Size: 5398 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3208 278 Size: 53 83 186 936 1672 2468 Fragments arranged by size: 2468 1672 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2741 1937 Size: 804 4594 Acc65I G'GTAC_C Cuts at: 2719 2719 Size: 5398 AclI AA'CG_TT Cuts at: 1563 3525 3898 1563 Size: 1962 373 3063 Fragments arranged by size: 3063 1962 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3461 4845 5113 1632 Size: 1829 1384 268 1917 Fragments arranged by size: 1917 1829 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5201 AflIII A'CryG_T Cuts at: 1203 2571 4297 5274 1203 Size: 1368 1726 977 1327 Fragments arranged by size: 1726 1368 1327 977 AgeI A'CCGG_T Cuts at: 4229 4229 Size: 5398 AhdI GACnn_n'nnGTC Cuts at: 4127 4127 Size: 5398 AleI CACnn'nnGTG Cuts at: 1166 2042 1166 Size: 876 4522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5398 AlwNI CAG_nnn'CTG Cuts at: 3141 4713 3141 Size: 1572 3826 ApaI G_GGCC'C Cuts at: 2512 2512 Size: 5398 ApaLI G'TGCA_C Cuts at: 2540 3455 4611 2540 Size: 915 1156 3327 Fragments arranged by size: 3327 1156 915 ApoI r'AATT_y Cuts at: 1493 2707 2933 3085 5194 1493 Size: 1214 226 152 2109 1697 Fragments arranged by size: 2109 1697 1214 226 152 AseI AT'TA_AT Cuts at: 160 1032 3952 160 Size: 872 2920 1606 Fragments arranged by size: 2920 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5398 AvaI C'yCGr_G Cuts at: 1870 1943 2397 2505 2723 1870 Size: 73 454 108 218 4545 Fragments arranged by size: 4545 454 218 108 73 BamHI G'GATC_C Cuts at: 2728 3095 2728 Size: 367 5031 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2309 2552 2719 4174 618 Size: 325 217 627 522 243 167 1455 1842 Fragments arranged by size: 1842 1455 627 522 325 243 217 167 BanII G_rGCy'C Cuts at: 1391 2187 2512 2626 2691 2717 1391 Size: 796 325 114 65 26 4072 Fragments arranged by size: 4072 796 325 114 65 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2122 928 Size: 971 223 4204 Fragments arranged by size: 4204 971 223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3589 3623 1903 Size: 34 1652 34 3678 Fragments arranged by size: 3678 1652 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3290 4500 3290 Size: 1210 4188 BclI T'GATC_A Cuts at: 1548 1548 Size: 5398 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2601 4009 136 Size: 107 122 71 631 605 929 1408 1525 Fragments arranged by size: 1525 1408 929 631 605 122 107 71 BglII A'GATC_T Cuts at: 2172 5393 2172 Size: 3221 2177 BlpI GC'TnA_GC Cuts at: 2815 2815 Size: 5398 Bme1580I G_kGCm'C Cuts at: 1790 2312 2512 2544 3459 4615 1790 Size: 522 200 32 915 1156 2573 Fragments arranged by size: 2573 1156 915 522 200 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5398 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3129 4087 442 Size: 416 53 83 399 24 1712 958 1753 Fragments arranged by size: 1753 1712 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5398 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 5398 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2705 4058 1239 Size: 1037 429 1353 2579 Fragments arranged by size: 2579 1353 1037 429 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3523 4388 4686 3523 Size: 865 298 4235 Fragments arranged by size: 4235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4061 882 Size: 617 2562 2219 Fragments arranged by size: 2562 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3189 493 Size: 711 1985 2702 Fragments arranged by size: 2702 1985 711 BsaBI GATnn'nnATC Cuts at: 5392 5392 Size: 5398 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3205 3587 4222 275 Size: 53 83 186 936 1672 382 635 1451 Fragments arranged by size: 1672 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3831 4229 4503 4650 5061 3831 Size: 398 274 147 411 4168 Fragments arranged by size: 4168 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5173 Fragments arranged by size: 5173 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2184 1186 Size: 844 154 4400 Fragments arranged by size: 4400 844 154 BseYI C'CCAG_C Cuts at: 2023 2410 4601 5097 2023 Size: 387 2191 496 2324 Fragments arranged by size: 2324 2191 496 387 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 2612 1163 Size: 573 330 546 3949 Fragments arranged by size: 3949 573 546 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2848 3609 4637 1540 Size: 275 186 847 761 1028 2301 Fragments arranged by size: 2301 1028 847 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2187 2544 2677 3459 3544 4615 2187 Size: 357 133 782 85 1071 2970 Fragments arranged by size: 2970 1071 782 357 133 85 BsmI GAATG_Cn' Cuts at: 2909 3002 2909 Size: 93 5305 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2312 2512 2544 2626 2677 2691 Size: 399 397 125 200 32 82 51 14 Cuts at: 2691 2717 3459 3544 4615 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 200 125 85 82 51 32 26 14 BspEI T'CCGG_A Cuts at: 5061 5061 Size: 5398 BspHI T'CATG_A Cuts at: 2566 3287 5017 2566 Size: 721 1730 2947 Fragments arranged by size: 2947 1730 721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2329 2592 2753 844 Size: 1485 263 161 3489 Fragments arranged by size: 3489 1485 263 161 BsrBI CCG'CTC Cuts at: 1394 1823 3285 1394 Size: 429 1462 3507 Fragments arranged by size: 3507 1462 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3893 4067 65 Size: 1183 2645 174 1396 Fragments arranged by size: 2645 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 4042 4229 1700 Size: 110 231 2001 187 2869 Fragments arranged by size: 2869 2001 231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5398 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5398 BssSI C'ACGA_G Cuts at: 3458 4470 3458 Size: 1012 4386 BstAPI GCAn_nnn'nTGC Cuts at: 794 2635 794 Size: 1841 3557 BstBI TT'CG_AA Cuts at: 4202 4202 Size: 5398 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5398 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2293 Size: 5398 BstYI r'GATC_y Cuts at: 1972 2172 2728 3095 3480 3497 4949 5035 5047 Size: 200 556 367 385 17 1452 86 12 Cuts at: 5047 5393 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 556 385 367 346 200 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2320 513 Size: 552 1255 3591 Fragments arranged by size: 3591 1255 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4659 Fragments arranged by size: 4659 683 56 BtsI GCAGTG_nn' Cuts at: 2914 3708 3728 2914 Size: 794 20 4584 Fragments arranged by size: 4584 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5363 DraI TTT'AAA Cuts at: 2702 2702 Size: 5398 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2147 4405 809 Size: 507 831 2258 1802 Fragments arranged by size: 2258 1802 831 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2497 2845 3734 Size: 54 1425 50 275 18 667 348 889 Cuts at: 3734 5363 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 667 348 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2845 1537 Size: 275 1033 4090 Fragments arranged by size: 4090 1033 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 3328 1353 Size: 839 1136 3423 Fragments arranged by size: 3423 1136 839 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3985 4359 4505 387 Size: 1064 138 2396 374 146 1280 Fragments arranged by size: 2396 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2185 2185 Size: 5398 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2705 3461 4058 4845 5113 1239 Size: 393 644 429 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 644 597 429 393 268 EcoRI G'AATT_C Cuts at: 2707 3085 2707 Size: 378 5020 FspI TGC'GCA Cuts at: 3904 3904 Size: 5398 HaeII r_GCGC'y Cuts at: 1678 2071 4545 1678 Size: 393 2474 2531 Fragments arranged by size: 2531 2474 393 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4039 4071 4113 4145 1373 Size: 32 135 32 2467 32 42 32 2626 Fragments arranged by size: 2626 2467 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2742 2988 669 Size: 988 281 804 246 3079 Fragments arranged by size: 3079 988 804 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5398 HpaI GTT'AAC Cuts at: 2988 2988 Size: 5398 KpnI G_GTAC'C Cuts at: 2723 2723 Size: 5398 MfeI C'AATT_G Cuts at: 2997 2997 Size: 5398 MluI A'CGCG_T Cuts at: 5274 5274 Size: 5398 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4512 4696 1305 Size: 3207 184 2007 Fragments arranged by size: 3207 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2499 10 Size: 54 1425 343 667 2909 Fragments arranged by size: 2909 1425 667 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2547 3356 3715 3874 518 Size: 648 876 505 809 359 159 2042 Fragments arranged by size: 2042 876 809 648 505 359 159 MspA1I CmG'CkG Cuts at: 1606 2814 3491 4639 4884 1606 Size: 1208 677 1148 245 2120 Fragments arranged by size: 2120 1208 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4846 NdeI CA'TA_TG Cuts at: 387 387 Size: 5398 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5398 NotI GC'GGCC_GC Cuts at: 2845 2845 Size: 5398 NspI r_CATG'y Cuts at: 1132 1229 2204 2243 2575 2756 4301 1132 Size: 97 975 39 332 181 1545 2229 Fragments arranged by size: 2229 1545 975 332 181 97 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5398 PciI A'CATG_T Cuts at: 2571 4297 2571 Size: 1726 3672 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5398 PmeI GTTT'AAAC Cuts at: 2702 2702 Size: 5398 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3467 3499 4980 5012 3467 Size: 32 1481 32 3853 Fragments arranged by size: 3853 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5398 PsiI TTA'TAA Cuts at: 2968 2968 Size: 5398 PspOMI G'GGCC_C Cuts at: 2508 2508 Size: 5398 PstI C_TGCA'G Cuts at: 830 2750 830 Size: 1920 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5398 SacI G_AGCT'C Cuts at: 2187 2187 Size: 5398 SalI G'TCGA_C Cuts at: 1936 2740 1936 Size: 804 4594 SapI GCTCTTCn'nnn_ Cuts at: 2192 2192 Size: 5398 SbfI CC_TGCA'GG Cuts at: 2750 2750 Size: 5398 ScaI AGT'ACT Cuts at: 3646 5358 3646 Size: 1712 3686 SfcI C'TryA_G Cuts at: 826 1045 2746 3881 4562 4753 826 Size: 219 1701 1135 681 191 1471 Fragments arranged by size: 1701 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5398 SgrAI Cr'CCGG_yG Cuts at: 1700 2041 1700 Size: 341 5057 SmaI CCC'GGG Cuts at: 1872 2399 2725 1872 Size: 527 326 4545 Fragments arranged by size: 4545 527 326 SmlI C'TyrA_G Cuts at: 820 1017 1943 3502 4403 4665 820 Size: 197 926 1559 901 262 1553 Fragments arranged by size: 1559 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3189 493 Size: 2696 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5398 SphI G_CATG'C Cuts at: 1132 2204 2756 1132 Size: 1072 552 3774 Fragments arranged by size: 3774 1072 552 SspI AAT'ATT Cuts at: 5 52 3322 5 Size: 47 3270 2081 Fragments arranged by size: 3270 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5398 StyI C'CwwG_G Cuts at: 513 1065 2145 2668 2837 513 Size: 552 1080 523 169 3074 Fragments arranged by size: 3074 1080 552 523 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2293 3425 3442 3595 3780 4225 325 Size: 1255 713 1132 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1132 713 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2242 3644 5356 Size: 274 80 33 51 1443 265 1402 1712 Cuts at: 5356 96 Size: 138 Fragments arranged by size: 1712 1443 1402 274 265 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5358 5390 5358 Size: 32 5366 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5398 XbaI T'CTAG_A Cuts at: 2734 2734 Size: 5398 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2293 2368 2293 Size: 75 5323 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5398 XmaI C'CCGG_G Cuts at: 1870 2397 2723 1870 Size: 527 326 4545 Fragments arranged by size: 4545 527 326 XmnI GAAnn'nnTTC Cuts at: 3527 3527 Size: 5398 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3206 276 Size: 53 83 186 936 1672 2468 Fragments arranged by size: 2468 1672 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI