(Circular) (Six-base) MAPSORT of: pFN21AB6942.seq Check: 6441 from: 1 to: 5392 pFN21AB6942 5392 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2152 2152 Size: 5392 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3202 278 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2735 1937 Size: 798 4594 Acc65I G'GTAC_C Cuts at: 2713 2713 Size: 5392 AclI AA'CG_TT Cuts at: 1563 3519 3892 1563 Size: 1956 373 3063 Fragments arranged by size: 3063 1956 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 3455 4839 5107 1632 Size: 1058 765 1384 268 1917 Fragments arranged by size: 1917 1384 1058 765 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5195 AflIII A'CryG_T Cuts at: 1203 4291 5268 1203 Size: 3088 977 1327 Fragments arranged by size: 3088 1327 977 AgeI A'CCGG_T Cuts at: 4223 4223 Size: 5392 AhdI GACnn_n'nnGTC Cuts at: 4121 4121 Size: 5392 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2517 1184 Size: 1333 4059 AlwNI CAG_nnn'CTG Cuts at: 2504 3135 4707 2504 Size: 631 1572 3189 Fragments arranged by size: 3189 1572 631 ApaI G_GGCC'C Cuts at: 2180 2180 Size: 5392 ApaLI G'TGCA_C Cuts at: 3449 4605 3449 Size: 1156 4236 ApoI r'AATT_y Cuts at: 1493 2701 2927 3079 5188 1493 Size: 1208 226 152 2109 1697 Fragments arranged by size: 2109 1697 1208 226 152 AseI AT'TA_AT Cuts at: 160 1032 3946 160 Size: 872 2914 1606 Fragments arranged by size: 2914 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 AvaI C'yCGr_G Cuts at: 1870 1943 2717 1870 Size: 73 774 4545 Fragments arranged by size: 4545 774 73 BamHI G'GATC_C Cuts at: 2722 3089 2722 Size: 367 5025 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2140 2713 4168 618 Size: 325 217 627 258 95 573 1455 1842 Fragments arranged by size: 1842 1455 627 573 325 258 217 95 BanII G_rGCy'C Cuts at: 1391 2180 2711 1391 Size: 789 531 4072 Fragments arranged by size: 4072 789 531 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3583 3617 1903 Size: 34 1646 34 3678 Fragments arranged by size: 3678 1646 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3284 4494 3284 Size: 1210 4182 BclI T'GATC_A Cuts at: 1548 1548 Size: 5392 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 2087 4003 Size: 107 122 71 631 605 382 33 1916 Cuts at: 4003 136 Size: 1525 Fragments arranged by size: 1916 1525 631 605 382 122 107 71 33 BglII A'GATC_T Cuts at: 5387 5387 Size: 5392 BlpI GC'TnA_GC Cuts at: 2809 2809 Size: 5392 Bme1580I G_kGCm'C Cuts at: 1790 2050 2143 2180 3453 4609 1790 Size: 260 93 37 1273 1156 2573 Fragments arranged by size: 2573 1273 1156 260 93 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5392 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3123 4081 442 Size: 416 53 83 399 24 1706 958 1753 Fragments arranged by size: 1753 1706 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5392 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 4052 1239 Size: 1193 1620 2579 Fragments arranged by size: 2579 1620 1193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3517 4382 4680 3517 Size: 865 298 4229 Fragments arranged by size: 4229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2555 4055 882 Size: 617 1056 1500 2219 Fragments arranged by size: 2219 1500 1056 617 BsaAI yAC'GTr Cuts at: 493 1204 2521 3183 493 Size: 711 1317 662 2702 Fragments arranged by size: 2702 1317 711 662 BsaBI GATnn'nnATC Cuts at: 5386 5386 Size: 5392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2678 3199 3581 4216 Size: 53 83 186 936 1145 521 382 635 Cuts at: 4216 275 Size: 1451 Fragments arranged by size: 1451 1145 936 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2466 3825 4223 4497 4644 5055 2466 Size: 1359 398 274 147 411 2803 Fragments arranged by size: 2803 1359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5167 Fragments arranged by size: 5167 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2342 2429 2546 2573 1186 Size: 892 264 87 117 27 4005 Fragments arranged by size: 4005 892 264 117 87 27 BseYI C'CCAG_C Cuts at: 2091 2592 4595 5091 2091 Size: 501 2003 496 2392 Fragments arranged by size: 2392 2003 501 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4819 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2842 3603 4631 1540 Size: 275 186 78 763 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2661 3453 3538 4609 2661 Size: 792 85 1071 3444 Fragments arranged by size: 3444 1071 792 85 BsmI GAATG_Cn' Cuts at: 2483 2903 2996 2483 Size: 420 93 4879 Fragments arranged by size: 4879 420 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2143 2180 2661 2711 3453 3538 Size: 399 260 93 37 481 50 742 85 Cuts at: 3538 4609 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 481 399 260 93 85 50 37 BspEI T'CCGG_A Cuts at: 5055 5055 Size: 5392 BspHI T'CATG_A Cuts at: 3281 5011 3281 Size: 1730 3662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 2747 844 Size: 1308 595 3489 Fragments arranged by size: 3489 1308 595 BsrBI CCG'CTC Cuts at: 1394 1823 2043 3279 1394 Size: 429 220 1236 3507 Fragments arranged by size: 3507 1236 429 220 BsrDI GCAATG_nn' Cuts at: 65 1248 2271 3887 4061 65 Size: 1183 1023 1616 174 1396 Fragments arranged by size: 1616 1396 1183 1023 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 4036 4223 1700 Size: 110 337 1889 187 2869 Fragments arranged by size: 2869 1889 337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5392 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5392 BssSI C'ACGA_G Cuts at: 2182 3452 4464 2182 Size: 1270 1012 3110 Fragments arranged by size: 3110 1270 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2273 2628 794 Size: 1479 355 3558 Fragments arranged by size: 3558 1479 355 BstBI TT'CG_AA Cuts at: 4196 4196 Size: 5392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5392 BstYI r'GATC_y Cuts at: 1972 2722 3089 3474 3491 4943 5029 5041 5387 Size: 750 367 385 17 1452 86 12 346 Cuts at: 5387 1972 Size: 1977 Fragments arranged by size: 1977 1452 750 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3901 Fragments arranged by size: 3901 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4653 Fragments arranged by size: 4653 683 56 BtsI GCAGTG_nn' Cuts at: 2633 2908 3702 3722 2633 Size: 275 794 20 4303 Fragments arranged by size: 4303 794 275 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5357 DraI TTT'AAA Cuts at: 2696 2696 Size: 5392 DraIII CAC_nnn'GTG Cuts at: 2577 2577 Size: 5392 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2683 4399 809 Size: 507 1367 1716 1802 Fragments arranged by size: 1802 1716 1367 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2839 3728 Size: 54 1425 50 275 18 246 763 889 Cuts at: 3728 5357 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 763 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2076 2839 1537 Size: 275 264 763 4090 Fragments arranged by size: 4090 763 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 3322 1353 Size: 1969 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 3979 4353 4499 387 Size: 1064 138 486 1904 374 146 1280 Fragments arranged by size: 1904 1280 1064 486 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 2690 3455 4052 4839 5107 1239 Size: 393 800 258 765 597 787 268 1524 Fragments arranged by size: 1524 800 787 765 597 393 268 258 EcoO109I rG'GnC_Cy Cuts at: 2068 2177 2407 2068 Size: 109 230 5053 Fragments arranged by size: 5053 230 109 EcoRI G'AATT_C Cuts at: 2701 3079 2701 Size: 378 5014 EcoRV GAT'ATC Cuts at: 2509 2509 Size: 5392 FspI TGC'GCA Cuts at: 3898 3898 Size: 5392 HaeII r_GCGC'y Cuts at: 1678 2431 4539 1678 Size: 753 2108 2531 Fragments arranged by size: 2531 2108 753 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 4033 4065 4107 Size: 32 135 32 776 32 1653 32 42 Cuts at: 4107 4139 1373 Size: 32 2626 Fragments arranged by size: 2626 1653 776 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 2982 669 Size: 988 281 798 246 3079 Fragments arranged by size: 3079 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5392 HpaI GTT'AAC Cuts at: 2982 2982 Size: 5392 KpnI G_GTAC'C Cuts at: 2717 2717 Size: 5392 MfeI C'AATT_G Cuts at: 2991 2991 Size: 5392 MluI A'CGCG_T Cuts at: 5268 5268 Size: 5392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 4506 4690 1305 Size: 711 2490 184 2007 Fragments arranged by size: 2490 2007 711 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3570 Fragments arranged by size: 3570 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3350 3709 3868 518 Size: 648 2184 359 159 2042 Fragments arranged by size: 2184 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2808 3485 4633 4878 1606 Size: 1202 677 1148 245 2120 Fragments arranged by size: 2120 1202 1148 677 245 NaeI GCC'GGC Cuts at: 2149 2149 Size: 5392 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3901 Fragments arranged by size: 3901 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5392 NgoMIV G'CCGG_C Cuts at: 2147 2147 Size: 5392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5392 NotI GC'GGCC_GC Cuts at: 2076 2839 2076 Size: 763 4629 NspI r_CATG'y Cuts at: 1132 1229 2750 4295 1132 Size: 97 1521 1545 2229 Fragments arranged by size: 2229 1545 1521 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5392 PciI A'CATG_T Cuts at: 4291 4291 Size: 5392 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5392 PmeI GTTT'AAAC Cuts at: 2696 2696 Size: 5392 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3461 3493 4974 5006 3461 Size: 32 1481 32 3847 Fragments arranged by size: 3847 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2407 2068 Size: 339 5053 PshAI GACnn'nnGTC Cuts at: 1613 2216 1613 Size: 603 4789 PsiI TTA'TAA Cuts at: 2962 2962 Size: 5392 PspOMI G'GGCC_C Cuts at: 2176 2176 Size: 5392 PstI C_TGCA'G Cuts at: 830 2744 830 Size: 1914 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5392 SalI G'TCGA_C Cuts at: 1936 2734 1936 Size: 798 4594 SbfI CC_TGCA'GG Cuts at: 2744 2744 Size: 5392 ScaI AGT'ACT Cuts at: 3640 5352 3640 Size: 1712 3680 SfcI C'TryA_G Cuts at: 826 1045 2740 3875 4556 4747 826 Size: 219 1695 1135 681 191 1471 Fragments arranged by size: 1695 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5392 SmaI CCC'GGG Cuts at: 1872 2719 1872 Size: 847 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3496 4397 4659 820 Size: 197 926 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3183 493 Size: 2690 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5392 SphI G_CATG'C Cuts at: 1132 2750 1132 Size: 1618 3774 SspI AAT'ATT Cuts at: 5 52 3316 5 Size: 47 3264 2081 Fragments arranged by size: 3264 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5392 StyI C'CwwG_G Cuts at: 513 1065 2004 2553 2831 513 Size: 552 939 549 278 3074 Fragments arranged by size: 3074 939 552 549 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3419 3436 3589 3774 4219 325 Size: 1255 1839 17 153 185 445 1498 Fragments arranged by size: 1839 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3638 5350 96 Size: 274 80 33 51 1443 1661 1712 138 Fragments arranged by size: 1712 1661 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5352 5384 5352 Size: 32 5360 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5392 XbaI T'CTAG_A Cuts at: 2728 2728 Size: 5392 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2407 2574 2407 Size: 167 5225 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5392 XmaI C'CCGG_G Cuts at: 1870 2717 1870 Size: 847 4545 XmnI GAAnn'nnTTC Cuts at: 3521 3521 Size: 5392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3200 276 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI